Restriction map for pF1KB3911
##################################
# NO CUT ENZYMES (  141 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
AlwNI       AocI        Aor51HI     ApaI        AscI        
AseI        AsnI        Asp718I     AspEI       AviII       
AvrII       BanI        BanII       BbeI        BbrPI       
BbvCI       BcgI        BcnI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BsmI        BspDI       
BspEI       BspLU11I    BsrBI       BsrDI       BsrFI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstXI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraII       DraIII      
DrdI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoT22I     FseI        FspI        HapII       
Hin1I       HincII      HindII      HindIII     HpaI        
HpaII       KasI        KpnI        Ksp632I     KspI        
MaeII       MamI        MluI        MroI        MspI        
NaeI        NarI        NciI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        SwaI        XbaI        XcmI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AccII          96  CGCG                 CG^CG
Alw44I         63  GTGCAC               G^TGCAC
ApaLI          63  GTGCAC               G^TGCAC
Asp700I       606  GAAGGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI          63  GTGCAC               G[AT]GC[AT]^C
AspI          574  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII         566  GGTCC                G^G[AT]CC
BalI          951  TGGCCA               TGG^CCA
BamHI         225  GGATCC               G^GATCC
BbsI         1209  GAAGAC               GAAGAC(2/6)
BclI          795  TGATCA               T^GATCA
BmyI           63  GTGCAC               G[AGT]GC[ACT]^C
BpmI          720  CTGGAG               CTGGAG(16/14)
BpuAI        1209  GAAGAC               GAAGAC(2/6)
BseRI          19  GAGGAG               GAGGAG(10/8)
BsiHKAI        63  GTGCAC               G[AT]GC[AT]^C
Bsp1286I       63  GTGCAC               G[AGT]GC[ACT]^C
BspHI         195  TCATGA               T^CATGA
BspMI         345  ACCTGC               ACCTGC(4/8)
BstAPI        969  GCAGTTTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI          96  CGCG                 CG^CG
BstYI         225  GGATCC               [GA]^GATC[CT]
Cac8I          99  GCCAGC               GC[ACGT]^[ACGT]GC
Cfr13I        566  GGTCC                G^G[ACGT]CC
DraI          150  TTTAAA               TTT^AAA
DsaI         1293  CCATGG               C^C[GA][CT]GG
EaeI          951  TGGCCA               [CT]^GGCC[GA]
EcoRI         610  GAATTC               G^AATTC
EcoRV         214  GATATC               GAT^ATC
FauI          696  GCGGG                CCCGC(4/6)
FbaI          795  TGATCA               T^GATCA
HaeII         894  AGCGCC               [GA]GCGC^[CT]
HgaI         1000  GACGC                GACGC(5/10)
MfeI          791  CAATTG               C^AATTG
MflI          225  GGATCC               [GA]^GATC[CT]
MluNI         951  TGGCCA               TGG^CCA
MscI          951  TGGCCA               TGG^CCA
MslI         1070  CACAGAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI          791  CAATTG               C^AATTG
MvnI           96  CGCG                 CG^CG
NcoI         1293  CCATGG               C^CATGG
PaeR7I       1004  CTCGAG               C^TCGAG
PflFI         574  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         557  CCATACAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI          195  TCATGA               T^CATGA
Sau96I        566  GGTCC                G^G[ACGT]CC
TaqI         1005  TCGA                 T^CGA
TfiI          394  GAATC                G^A[AT]TC
Tth111I       574  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I      1005  TCGA                 T^CGA
Van91I        557  CCATACAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         1004  CTCGAG               C^TCGAG
XhoII         225  GGATCC               [GA]^GATC[CT]
XmnI          606  GAAGGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AvaI          704  CTCGGG               C^[CT]CG[GA]G
             1004  CTCGAG              
BciVI         542  GGATAC               GTATCC(6/5)
             1222  GGATAC              
BfaI          163  CTAG                 C^TAG
              979  CTAG                
BsgI          503  CTGCAC               GTGCAG(16/14)
             1229  GTGCAG              
BsiYI         557  CCATACAGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              582  CCTGCATTGGG         
BslI          557  CCATACAGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              582  CCTGCATTGGG         
BsmAI        1007  GAGAC                GTCTC(1/5)
              671  GTCTC               
BsmFI        1080  GGGAC                GGGAC(10/14)
             1151  GGGAC               
BsoBI         704  CTCGGG               C^[CT]CG[GA]G
             1004  CTCGAG              
BssKI         117  CCAGG                ^CC[ACGT]GG
              928  CCTGG               
BstF5I        327  CATCC                GGATG(2/0)
              189  GGATG               
BstNI         117  CCAGG                CC^[AT]GG
              928  CCTGG               
CfoI           97  GCGC                 GCG^C
              895  GCGC                
DdeI          280  CTTAG                C^T[ACGT]AG
             1120  CTCAG               
EcoRII        117  CCAGG                ^CC[AT]GG
              928  CCTGG               
FokI          327  CATCC                GGATG(9/13)
              189  GGATG               
HaeIII        120  GGCC                 GG^CC
              952  GGCC                
HhaI           97  GCGC                 GCG^C
              895  GCGC                
HinP1I         97  GCGC                 G^CGC
              895  GCGC                
MaeI          163  CTAG                 C^TAG
              979  CTAG                
MvaI          117  CCAGG                CC^[AT]GG
              928  CCTGG               
NlaIV         225  GGATCC               GG[ACGT]^[ACGT]CC
              699  GGTTCC              
PleI         1212  GACTC                GAGTC(4/5)
              723  GAGTC               
PvuII         101  CAGCTG               CAG^CTG
              220  CAGCTG              
ScrFI         117  CCAGG                CC^[ACGT]GG
              928  CCTGG               
SfcI          175  CTACAG               C^T[GA][CT]AG
              516  CTATAG              
SmlI          422  CTCAAG               C^T[CT][GA]AG
             1004  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   36 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        BbvI        BsaJI       Bsp1407I    BsrGI       
BsrI        DpnII       EcoT14I     Fnu4HI      HinfI       
HphI        ItaI        MaeIII      MboI        MboII       
MnlI        MseI        MspA1I      MwoI        NdeII       
NlaIII      RsaI        Sau3AI      SfaNI       SspBI       
StyI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com