Restriction map for pF1KB3881
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# NO CUT ENZYMES (  138 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AflII       
AflIII      AgeI        AhdI        AlwNI       AocI        
Aor51HI     ApaI        ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AvaI        
AvrII       BalI        BanI        BbeI        BbrPI       
BbsI        BciVI       BclI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaBI       BsaI        BsgI        
BsiEI       BsiWI       BsmBI       BsmI        BsoBI       
BspDI       BspLU11I    BspMI       BsrFI       BssSI       
BstBI       BstEII      BstPI       BstXI       Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DraII       DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        HaeII       HpaI        KasI        KpnI        
KspI        MaeI        MamI        MfeI        MluI        
MluNI       MscI        MseI        MunI        NaeI        
NarI        NcoI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
PvuII       RsrII       SacI        SacII       SalI        
SapI        SbfI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SspI        SwaI        TaqI        Tru9I       Tsp509I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccI          649  GTATAC               GT^[AC][GT]AC
AccIII        596  TCCGGA               T^CCGGA
AcyI         1012  GACGCC               G[GA]^CG[CT]C
AspI          340  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         848  TGCGCA               TGC^GCA
BanII         978  GAGCCC               G[GA]GC[CT]^C
BbvCI         232  GCTGAGG              CCTCAGC(-5/-2)
BcgI          454  CGAGTGCACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI          70  TACGTG               [CT]AC^GT[GA]
BsaHI        1012  GACGCC               G[GA]^CG[CT]C
BseAI         596  TCCGGA               T^CCGGA
BsmAI        1018  GAGAC                GTCTC(1/5)
Bsp1407I      206  TGTACA               T^GTACA
BspEI         596  TCCGGA               T^CCGGA
BspHI         521  TCATGA               T^CATGA
BsrGI         206  TGTACA               T^GTACA
Bst1107I      649  GTATAC               GTA^TAC
BstZ17I       649  GTATAC               GTA^TAC
DrdI          574  GACTGTCTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          502  CGGCCA               [CT]^GGCC[GA]
EarI          190  CTCTTC               CTCTTC(1/4)
EcoT14I       764  CCAAGG               C^C[AT][AT]GG
FauI          774  GCGGG                CCCGC(4/6)
FspI          848  TGCGCA               TGC^GCA
Hin1I        1012  GACGCC               G[GA]^CG[CT]C
HindIII      1027  AAGCTT               A^AGCTT
Ksp632I       190  CTCTTC               CTCTTC(1/4)
MroI          596  TCCGGA               T^CCGGA
NdeI          871  CATATG               CA^TATG
PflFI         340  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PstI         1075  CTGCAG               CTGCA^G
RcaI          521  TCATGA               T^CATGA
ScaI          404  AGTACT               AGT^ACT
SexAI          95  ACCAGGT              A^CC[AT]GGT
SfcI         1075  CTGCAG               C^T[GA][CT]AG
SmlI          883  CTCAAG               C^T[CT][GA]AG
SphI         1061  GCATGC               GCATG^C
SspBI         206  TGTACA               T^GTACA
StyI          764  CCAAGG               C^C[AT][AT]GG
Tth111I       340  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

BamHI         614  GGATCC               G^GATCC
              641  GGATCC              
BsaWI         215  ACCGGA               [AT]^CCGG[AT]
              596  TCCGGA              
BsmFI         338  GGGAC                GGGAC(10/14)
              583  GTCCC               
BsrBI         420  GAGCGG               CCGCTC(-3/-3)
              610  GAGCGG              
BsrDI         530  CATTGC               GCAATG(2/0)
              829  CATTGC              
BssHII        722  GCGCGC               G^CGCGC
              923  GCGCGC              
DraIII        271  CACACGGTG            CAC[ACGT][ACGT][ACGT]^GTG
              354  CACCGAGTG           
HgaI          340  GACGC                GACGC(5/10)
             1012  GACGC               
HincII        346  GTCAAC               GT[CT]^[GA]AC
              544  GTCAAC              
HindII        346  GTCAAC               GT[CT]^[GA]AC
              544  GTCAAC              
MaeIII        668  GTGAC                ^GT[ACGT]AC
              892  GTGAC               
MboII         117  GAAGA                GAAGA(8/7)
              191  TCTTC               
MspA1I        772  CAGCGG               C[AC]G^C[GT]G
              997  CCGCTG              
NspI          854  ACATGC               [GA]CATG^[CT]
             1061  GCATGC              
StuI            9  AGGCCT               AGG^CCT
              159  AGGCCT              
TfiI          261  GATTC                G^A[AT]TC
              332  GATTC               
Tsp45I        668  GTGAC                ^GT[GC]AC
              892  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   63 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        Alw44I      
AlwI        ApaLI       AspHI       AvaII       BbvI        
BcnI        BmyI        BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        Bsp1286I    BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
EcoRII      Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeII       MboI        MflI        MnlI        
MslI        MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
TseI        TspRI       XhoII       
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