Restriction map for pF1KB3881
 (Linear) (Six-base) MAPSORT of: KIBB3881.seq  Check: 998  from: 1  to: 1107



KIBB3881.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:56  ..

AccI GT'mk_AC

Cuts at:      0     650    1107 
   Size:        650     457
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     325     625     733    1107 
   Size:        325     300     108     374

  Fragments arranged by size: 

                 374     325     300     108
 
AleI CACnn'nnGTG

Cuts at:      0     316     754    1107 
   Size:        316     438     353

  Fragments arranged by size: 

                 438     353     316
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      28     889    1107 
   Size:         28     861     218

  Fragments arranged by size: 

                 861     218      28
 
ApaLI G'TGCA_C

Cuts at:      0     100     457     904    1107 
   Size:        100     357     447     203

  Fragments arranged by size: 

                 447     357     203     100
 
BamHI G'GATC_C

Cuts at:      0     614     641    1107 
   Size:        614      27     466

  Fragments arranged by size: 

                 614     466      27
 
BanII G_rGCy'C

Cuts at:      0     982    1107 
   Size:        982     125
 
BbvCI CC'TCA_GC

Cuts at:      0     233    1107 
   Size:        233     874
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     443     477    1107 
   Size:        443      34     630

  Fragments arranged by size: 

                 630     443      34
 
Bme1580I G_kGCm'C

Cuts at:      0     104     461     908    1107 
   Size:        104     357     447     199

  Fragments arranged by size: 

                 447     357     199     104
 
BmgBI CAC'GTC

Cuts at:      0     891     896     903    1107 
   Size:        891       5       7     204

  Fragments arranged by size: 

                 891     204       7       5
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     581    1107 
   Size:        581     526
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     687    1107 
   Size:        687     420
 
Bpu10I CC'TnA_GC

Cuts at:      0     233    1107 
   Size:        233     874
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     868    1107 
   Size:        868     239
 
BsaAI yAC'GTr

Cuts at:      0      72    1107 
   Size:         72    1035
 
BsaHI Gr'CG_yC

Cuts at:      0    1013    1107 
   Size:       1013      94
 
BsaWI w'CCGG_w

Cuts at:      0     215     596    1107 
   Size:        215     381     511

  Fragments arranged by size: 

                 511     381     215
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     463     493    1107 
   Size:        463      30     614

  Fragments arranged by size: 

                 614     463      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     250     301     637    1107 
   Size:        250      51     336     470

  Fragments arranged by size: 

                 470     336     250      51
 
BsiHKAI G_wGCw'C

Cuts at:      0       7     104     461     908    1107 
   Size:          7      97     357     447     199

  Fragments arranged by size: 

                 447     357     199      97       7
 
Bsp1286I G_dGCh'C

Cuts at:      0       7     104     461     908     982    1107 
   Size:          7      97     357     447      74     125

  Fragments arranged by size: 

                 447     357     125      97      74       7
 
BspEI T'CCGG_A

Cuts at:      0     596    1107 
   Size:        596     511
 
BspHI T'CATG_A

Cuts at:      0     521    1107 
   Size:        521     586
 
BsrBI CCG'CTC

Cuts at:      0     422     612    1107 
   Size:        422     190     495

  Fragments arranged by size: 

                 495     422     190
 
BsrDI GCAATG_nn'

Cuts at:      0     529     828    1107 
   Size:        529     299     279

  Fragments arranged by size: 

                 529     299     279
 
BsrGI T'GTAC_A

Cuts at:      0     206    1107 
   Size:        206     901
 
BssHII G'CGCG_C

Cuts at:      0     722     923    1107 
   Size:        722     201     184

  Fragments arranged by size: 

                 722     201     184
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     498    1071    1095    1107 
   Size:        498     573      24      12

  Fragments arranged by size: 

                 573     498      24      12
 
BstYI r'GATC_y

Cuts at:      0     593     614     641    1107 
   Size:        593      21      27     466

  Fragments arranged by size: 

                 593     466      27      21
 
BstZ17I GTA'TAC

Cuts at:      0     651    1107 
   Size:        651     456
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     546    1107 
   Size:        546     561
 
BtsI GCAGTG_nn'

Cuts at:      0      85     459     927    1107 
   Size:         85     374     468     180

  Fragments arranged by size: 

                 468     374     180      85
 
DraIII CAC_nnn'GTG

Cuts at:      0     276     359    1107 
   Size:        276      83     748

  Fragments arranged by size: 

                 748     276      83
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     580    1107 
   Size:        580     527
 
EaeI y'GGCC_r

Cuts at:      0     502    1107 
   Size:        502     605
 
EarI CTCTTCn'nnn_

Cuts at:      0     196    1107 
   Size:        196     911
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     325     625     733    1107 
   Size:        325     300     108     374

  Fragments arranged by size: 

                 374     325     300     108
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     141    1107 
   Size:        141     966
 
FspI TGC'GCA

Cuts at:      0     850    1107 
   Size:        850     257
 
HincII GTy'rAC

Cuts at:      0     348     546    1107 
   Size:        348     198     561

  Fragments arranged by size: 

                 561     348     198
 
HindIII A'AGCT_T

Cuts at:      0    1027    1107 
   Size:       1027      80
 
MslI CAynn'nnrTG

Cuts at:      0     139     316     754     871    1107 
   Size:        139     177     438     117     236

  Fragments arranged by size: 

                 438     236     177     139     117
 
MspA1I CmG'CkG

Cuts at:      0     774     999    1107 
   Size:        774     225     108

  Fragments arranged by size: 

                 774     225     108
 
NdeI CA'TA_TG

Cuts at:      0     872    1107 
   Size:        872     235
 
NspI r_CATG'y

Cuts at:      0     858    1065    1107 
   Size:        858     207      42

  Fragments arranged by size: 

                 858     207      42
 
PasI CC'CwG_GG

Cuts at:      0     797    1107 
   Size:        797     310
 
PfoI T'CCnGG_A

Cuts at:      0     617    1107 
   Size:        617     490
 
PstI C_TGCA'G

Cuts at:      0    1079    1107 
   Size:       1079      28
 
ScaI AGT'ACT

Cuts at:      0     406    1107 
   Size:        406     701
 
SexAI A'CCwGG_T

Cuts at:      0      95    1107 
   Size:         95    1012
 
SfcI C'TryA_G

Cuts at:      0    1075    1107 
   Size:       1075      32
 
SmlI C'TyrA_G

Cuts at:      0     883    1107 
   Size:        883     224
 
SphI G_CATG'C

Cuts at:      0    1065    1107 
   Size:       1065      42
 
StuI AGG'CCT

Cuts at:      0      11     161    1107 
   Size:         11     150     946

  Fragments arranged by size: 

                 946     150      11
 
StyI C'CwwG_G

Cuts at:      0     764    1107 
   Size:        764     343
 
TatI w'GTAC_w

Cuts at:      0     206     404    1107 
   Size:        206     198     703

  Fragments arranged by size: 

                 703     206     198
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     880     912    1107 
   Size:        880      32     195

  Fragments arranged by size: 

                 880     195      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     343    1107 
   Size:        343     764
 

 Enzymes that do cut:

     AccI     AcuI     AleI     AlfI    ApaLI    BamHI    BanII    BbvCI
     BcgI Bme1580I    BmgBI     BmrI     BplI   Bpu10I    BpuEI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI  BsiHKAI Bsp1286I    BspEI    BspHI
    BsrBI    BsrDI    BsrGI   BssHII   BstAPI    BstYI  BstZ17I    BtgZI
     BtsI   DraIII     DrdI     EaeI     EarI  Eco57MI     FalI     FspI
   HincII  HindIII     MslI   MspA1I     NdeI     NspI     PasI     PfoI
     PstI     ScaI    SexAI     SfcI     SmlI     SphI     StuI     StyI
     TatI     TstI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AloI    AlwNI     ApaI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI     BanI     BbeI     BbsI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI     BmtI     BpmI     BsaI    BsaBI
    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspMI    BsrFI
    BssSI    BstBI   BstEII    BstXI   Bsu36I     BtgI     ClaI    CspCI
     DraI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FseI    FspAI    HaeII    Hin4I     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     NaeI     NarI     NcoI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI
     SspI     SwaI    TaqII     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI
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