Restriction map for pF1KB3845
##################################
# NO CUT ENZYMES (  140 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AvaI        AvrII       
BamHI       BbeI        BbrPI       BciVI       BclI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BseAI       BsgI        BsiHKAI     BsiWI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspLU11I    BsrBI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DraIII      Eam1105I    Eco47III    
Eco81I      EcoO65I     EcoRV       EcoT22I     FauI        
FbaI        FseI        HaeII       Hin1I       HindIII     
HinfI       HpaI        KasI        KpnI        KspI        
MaeI        MaeII       MamI        MfeI        MluI        
MroI        MseI        MunI        MvnI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PleI        PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        SwaI        TfiI        Tru9I       
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AgeI          172  ACCGGT               A^CCGGT
AspI          904  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         152  TGCGCA               TGC^GCA
BalI          201  TGGCCA               TGG^CCA
BbvCI         585  CCTCAGC              CCTCAGC(-5/-2)
BcgI          663  GCAAAGGGTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI          282  CCGGG                CC^[GC]GG
BsiEI         834  CGGCCG               CG[GA][CT]^CG
BspHI         575  TCATGA               T^CATGA
BspMI         110  ACCTGC               ACCTGC(4/8)
BsrDI         615  CATTGC               GCAATG(2/0)
BsrFI         172  ACCGGT               [GA]^CCGG[CT]
BsrI          213  ACTGG                ACTGG(1/-1)
BssSI         506  CTCGTG               CACGAG(-5/-1)
CfoI          153  GCGC                 GCG^C
Cfr10I        172  ACCGGT               [GA]^CCGG[CT]
DrdI          502  GACACTCGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          847  CCATGG               C^C[GA][CT]GG
EagI          834  CGGCCG               C^GGCCG
EarI          241  CTCTTC               CTCTTC(1/4)
EclXI         834  CGGCCG               C^GGCCG
Eco52I        834  CGGCCG               C^GGCCG
EcoT14I       847  CCATGG               C^C[AT][AT]GG
FspI          152  TGCGCA               TGC^GCA
HgaI           31  GACGC                GACGC(5/10)
HhaI          153  GCGC                 GCG^C
HinP1I        153  GCGC                 G^CGC
HphI         1011  GGTGA                GGTGA(8/7)
Ksp632I       241  CTCTTC               CTCTTC(1/4)
MluNI         201  TGGCCA               TGG^CCA
MscI          201  TGGCCA               TGG^CCA
MslI          858  CATCGAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI          282  CCGGG                CC^[GC]GG
NcoI          847  CCATGG               C^CATGG
PflFI         904  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         379  CCAGGCTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         172  ACCGGT               A^CCGGT
PvuII         273  CAGCTG               CAG^CTG
RcaI          575  TCATGA               T^CATGA
SapI          240  GCTCTTC              GCTCTTC(1/4)
SphI          773  GCATGC               GCATG^C
StyI          847  CCATGG               C^C[AT][AT]GG
Tth111I       904  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        379  CCAGGCTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI           38  CCATGTCAGAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

BanI           51  GGTGCC               G^G[CT][GA]CC
               78  GGTGCC              
BbsI          159  GAAGAC               GAAGAC(2/6)
              168  GAAGAC              
BbvI           34  GCAGC                GCAGC(8/12)
              518  GCTGC               
BpuAI         159  GAAGAC               GAAGAC(2/6)
              168  GAAGAC              
BsaI           19  GAGACC               GGTCTC(1/5)
              389  GAGACC              
BsaWI         172  ACCGGT               [AT]^CCGG[AT]
             1008  TCCGGT              
BstYI         293  AGATCC               [GA]^GATC[CT]
              564  GGATCT              
Cac8I          72  GCCAGC               GC[ACGT]^[ACGT]GC
              773  GCATGC              
EaeI          201  TGGCCA               [CT]^GGCC[GA]
              834  CGGCCG              
EcoNI         459  CCTGAAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              585  CCTCAGCAAGG         
EcoRI         655  GAATTC               G^AATTC
              700  GAATTC              
HincII        232  GTCAAC               GT[CT]^[GA]AC
              472  GTCAAC              
HindII        232  GTCAAC               GT[CT]^[GA]AC
              472  GTCAAC              
MflI          293  AGATCC               [GA]^GATC[CT]
              564  GGATCT              
MspA1I         74  CAGCGG               C[AC]G^C[GT]G
              273  CAGCTG              
NspI          327  GCATGT               [GA]CATG^[CT]
              773  GCATGC              
SmlI          568  CTTGAG               C^T[CT][GA]AG
              934  CTCAAG              
TseI           34  GCAGC                G^C[AT]GC
              518  GCTGC               
Tsp45I        797  GTGAC                ^GT[GC]AC
              943  GTGAC               
XhoII         293  AGATCC               [GA]^GATC[CT]
              564  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   53 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AvaII       BanII       BmyI        BpmI        
BsaJI       BseRI       BsiYI       BslI        BsmAI       
Bsp1286I    BssKI       BstF5I      BstNI       BstXI       
Cfr13I      DdeI        DpnII       DraII       EcoO109I    
EcoRII      Fnu4HI      FokI        HaeIII      HapII       
HpaII       ItaI        MaeIII      MboI        MboII       
MnlI        MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       PpuMI       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       StuI        TaqI        
Tsp509I     TspRI       TthHB8I     
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