Restriction map for pF1KB3754
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# NO CUT ENZYMES (  119 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcyI        AgeI        AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AvaI        AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbrPI       BcgI        BciVI       BclI        BcnI        
BglI        BlnI        BsaAI       BsaHI       BsiEI       
BsiWI       BsmI        BsoBI       BspLU11I    BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstZ17I     CpoI        DraI        
DrdI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO65I     EcoRV       
FbaI        FseI        FspI        HaeII       Hin1I       
HincII      HindII      HpaI        KasI        KpnI        
KspI        MfeI        MluI        MluNI       MscI        
MunI        MvnI        NaeI        NarI        NciI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SspI        StuI        SwaI        Van91I      XbaI        
XhoI        XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccIII        360  TCCGGA               T^CCGGA
AflII        1656  CTTAAG               C^TTAAG
AocI         1058  CCTCAGG              CC^T[ACGT]AGG
AspI         1957  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI        1180  CCTCAGC              CCTCAGC(-5/-2)
BfrI         1656  CTTAAG               C^TTAAG
BglII          75  AGATCT               A^GATCT
BlpI         1249  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1249  GCTGAGC              GC^T[ACGT]AGC
BsaI         1462  GGTCTC               GGTCTC(1/5)
BseAI         360  TCCGGA               T^CCGGA
BsgI         1927  GTGCAG               GTGCAG(16/14)
BsmBI        1169  CGTCTC               CGTCTC(1/5)
Bsp1407I     1620  TGTACA               T^GTACA
BspDI        1216  ATCGAT               AT^CGAT
BspEI         360  TCCGGA               T^CCGGA
BspHI        1466  TCATGA               T^CATGA
BspMI         882  ACCTGC               ACCTGC(4/8)
BsrBI        1503  CCGCTC               CCGCTC(-3/-3)
BsrDI         182  CATTGC               GCAATG(2/0)
BsrGI        1620  TGTACA               T^GTACA
BstXI        1355  CCACCCTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1058  CCTCAGG              CC^T[ACGT]AGG
CelII        1249  GCTGAGC              GC^T[ACGT]AGC
ClaI         1216  ATCGAT               AT^CGAT
DraII         549  AGGTCCT              [GA]G^G[ACGT]CC[CT]
Eco81I       1058  CCTCAGG              CC^T[ACGT]AGG
EcoO109I      549  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoRI        1725  GAATTC               G^AATTC
EcoT22I       902  ATGCAT               ATGCA^T
MroI          360  TCCGGA               T^CCGGA
NdeI          162  CATATG               CA^TATG
NsiI          902  ATGCAT               ATGCA^T
PflFI        1957  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         549  AGGTCCT              [GA]G^G[AT]CC[CT]
PstI         1679  CTGCAG               CTGCA^G
RcaI         1466  TCATGA               T^CATGA
SacI         1981  GAGCTC               GAGCT^C
SexAI         152  ACCTGGT              A^CC[AT]GGT
SspBI        1620  TGTACA               T^GTACA
Tth111I      1957  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          473  CCAGTGGGGTGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AflIII        230  ACGTGT               A^C[GA][CT]GT
              866  ACACGT              
BanII          68  GGGCTC               G[GA]GC[CT]^C
             1981  GAGCTC              
BbsI           44  GAAGAC               GAAGAC(2/6)
              709  GTCTTC              
BpuAI          44  GAAGAC               GAAGAC(2/6)
              709  GTCTTC              
BsaBI          76  GATCTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1912  GATGCAGATC          
BsrFI         826  GCCGGT               [GA]^CCGG[CT]
             1103  ACCGGC              
BsrI          473  CCAGT                ACTGG(1/-1)
             1452  CCAGT               
CfoI         1389  GCGC                 GCG^C
             1831  GCGC                
Cfr10I        826  GCCGGT               [GA]^CCGG[CT]
             1103  ACCGGC              
EarI          677  CTCTTC               CTCTTC(1/4)
             1164  CTCTTC              
EcoT14I        32  CCATGG               C^C[AT][AT]GG
              369  CCATGG              
HaeIII        367  GGCC                 GG^CC
             1532  GGCC                
HgaI         1578  GACGC                GACGC(5/10)
             1558  GCGTC               
HhaI         1389  GCGC                 GCG^C
             1831  GCGC                
HinP1I       1389  GCGC                 G^CGC
             1831  GCGC                
Ksp632I       677  CTCTTC               CTCTTC(1/4)
             1164  CTCTTC              
MamI           76  GATCTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1912  GATGCAGATC          
MslI          633  CACGCTTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              823  CATGCCGGTG          
NcoI           32  CCATGG               C^CATGG
              369  CCATGG              
NlaIV         210  GGACCC               GG[ACGT]^[ACGT]CC
             1376  GGCTCC              
SfcI          102  CTACAG               C^T[GA][CT]AG
             1679  CTGCAG              
SphI          112  GCATGC               GCATG^C
             1085  GCATGC              
StyI           32  CCATGG               C^C[AT][AT]GG
              369  CCATGG              
TaqI          687  TCGA                 T^CGA
             1217  TCGA                
TfiI         1684  GATTC                G^A[AT]TC
             1762  GATTC               
Tsp45I        610  GTCAC                ^GT[GC]AC
              782  GTCAC               
TthHB8I       687  TCGA                 T^CGA
             1217  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   70 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AspHI       AvaII       BbvI        
BfaI        BmyI        BpmI        BsaJI       BsaWI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DraIII      DsaI        EcoRII      FauI        Fnu4HI      
FokI        HapII       HindIII     HinfI       HpaII       
HphI        ItaI        MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MspA1I      MspI        MvaI        MwoI        NdeII       
NlaIII      NspI        PleI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SmlI        
Tru9I       TseI        Tsp509I     TspRI       XhoII       
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