Restriction map for pF1KB3754
 (Linear) (Six-base) MAPSORT of: KIBB3754.seq  Check: 2637  from: 1  to: 2049



KIBB3754.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:56  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     891    2049 
   Size:        891    1158
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1955    2049 
   Size:       1955      94
 
AflII C'TTAA_G

Cuts at:      0    1656    2049 
   Size:       1656     393
 
AflIII A'CryG_T

Cuts at:      0     230     866    2049 
   Size:        230     636    1183

  Fragments arranged by size: 

                1183     636     230
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1208    1262    1856    2049 
   Size:       1208      54     594     193

  Fragments arranged by size: 

                1208     594     193      54
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1361    1393    2049 
   Size:       1361      32     656

  Fragments arranged by size: 

                1361     656      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     587     663    2013    2049 
   Size:        587      76    1350      36

  Fragments arranged by size: 

                1350     587      76      36
 
ApoI r'AATT_y

Cuts at:      0     377    1725    1768    2049 
   Size:        377    1348      43     281

  Fragments arranged by size: 

                1348     377     281      43
 
BanII G_rGCy'C

Cuts at:      0      72    1985    2049 
   Size:         72    1913      64

  Fragments arranged by size: 

                1913      72      64
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      51     702    2049 
   Size:         51     651    1347

  Fragments arranged by size: 

                1347     651      51
 
BbvCI CC'TCA_GC

Cuts at:      0    1181    2049 
   Size:       1181     868
 
BfrBI ATG'CAT

Cuts at:      0     904    2049 
   Size:        904    1145
 
BglII A'GATC_T

Cuts at:      0      75    2049 
   Size:         75    1974
 
BlpI GC'TnA_GC

Cuts at:      0    1250    2049 
   Size:       1250     799
 
BmgBI CAC'GTC

Cuts at:      0     869    2049 
   Size:        869    1180
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1211    2049 
   Size:       1211     838
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     456     834    1408    1969    1993    2049 
   Size:        456     378     574     561      24      56

  Fragments arranged by size: 

                 574     561     456     378      56      24
 
Bpu10I CC'TnA_GC

Cuts at:      0     121     919    1181    2049 
   Size:        121     798     262     868

  Fragments arranged by size: 

                 868     798     262     121
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      56    1111    2049 
   Size:         56    1055     938

  Fragments arranged by size: 

                1055     938      56
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1468    2049 
   Size:       1468     581
 
BsaBI GATnn'nnATC

Cuts at:      0      80    1916    2049 
   Size:         80    1836     133

  Fragments arranged by size: 

                1836     133      80
 
BsaWI w'CCGG_w

Cuts at:      0     360     717    1550    2049 
   Size:        360     357     833     499

  Fragments arranged by size: 

                 833     499     360     357
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1361    1391    2049 
   Size:       1361      30     658

  Fragments arranged by size: 

                1361     658      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     319    1047    1369    1497    2049 
   Size:        319     728     322     128     552

  Fragments arranged by size: 

                 728     552     322     319     128
 
BseYI C'CCAG_C

Cuts at:      0    1513    2049 
   Size:       1513     536
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1948    2049 
   Size:       1948     101
 
BsiHKAI G_wGCw'C

Cuts at:      0     254    1197    1985    2049 
   Size:        254     943     788      64

  Fragments arranged by size: 

                 943     788     254      64
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1175    2049 
   Size:       1175     874
 
Bsp1286I G_dGCh'C

Cuts at:      0      72     254    1197    1985    2049 
   Size:         72     182     943     788      64

  Fragments arranged by size: 

                 943     788     182      72      64
 
BspEI T'CCGG_A

Cuts at:      0     360    2049 
   Size:        360    1689
 
BspHI T'CATG_A

Cuts at:      0    1466    2049 
   Size:       1466     583
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     891    2049 
   Size:        891    1158
 
BsrBI CCG'CTC

Cuts at:      0    1505    2049 
   Size:       1505     544
 
BsrDI GCAATG_nn'

Cuts at:      0     181    2049 
   Size:        181    1868
 
BsrFI r'CCGG_y

Cuts at:      0     826    1103    2049 
   Size:        826     277     946

  Fragments arranged by size: 

                 946     826     277
 
BsrGI T'GTAC_A

Cuts at:      0    1620    2049 
   Size:       1620     429
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1362    2049 
   Size:       1362     687
 
BstYI r'GATC_y

Cuts at:      0      75     583     645    1286    1791    1917    2049 
   Size:         75     508      62     641     505     126     132

  Fragments arranged by size: 

                 641     508     505     132     126      75      62
 
Bsu36I CC'TnA_GG

Cuts at:      0    1059    2049 
   Size:       1059     990
 
BtgI C'CryG_G

Cuts at:      0      32     369     533    2049 
   Size:         32     337     164    1516

  Fragments arranged by size: 

                1516     337     164      32
 
BtsI GCAGTG_nn'

Cuts at:      0     664    2049 
   Size:        664    1385
 
ClaI AT'CG_AT

Cuts at:      0    1217    2049 
   Size:       1217     832
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     968    1003    2049 
   Size:        968      35    1046

  Fragments arranged by size: 

                1046     968      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     156     416     999    2049 
   Size:        156     260     583    1050

  Fragments arranged by size: 

                1050     583     260     156
 
EarI CTCTTCn'nnn_

Cuts at:      0     683    1170    2049 
   Size:        683     487     879

  Fragments arranged by size: 

                 879     683     487
 
EcoICRI GAG'CTC

Cuts at:      0    1983    2049 
   Size:       1983      66
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     456     834    1408    1955    1969    1993    2049 
   Size:        456     378     574     547      14      24      56

  Fragments arranged by size: 

                 574     547     456     378      56      24      14
 
EcoO109I rG'GnC_Cy

Cuts at:      0     550    2049 
   Size:        550    1499
 
EcoRI G'AATT_C

Cuts at:      0    1725    2049 
   Size:       1725     324
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      54      86     413     445     853     885    2049 
   Size:         54      32     327      32     408      32    1164

  Fragments arranged by size: 

                1164     408     327      54      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0     118    1316    2024    2049 
   Size:        118    1198     708      25

  Fragments arranged by size: 

                1198     708     118      25
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     114    1436    2049 
   Size:        114    1322     613

  Fragments arranged by size: 

                1322     613     114
 
MslI CAynn'nnrTG

Cuts at:      0     637     827    2049 
   Size:        637     190    1222

  Fragments arranged by size: 

                1222     637     190
 
MspA1I CmG'CkG

Cuts at:      0      51    1242    1971    2049 
   Size:         51    1191     729      78

  Fragments arranged by size: 

                1191     729      78      51
 
NcoI C'CATG_G

Cuts at:      0      32     369    2049 
   Size:         32     337    1680

  Fragments arranged by size: 

                1680     337      32
 
NdeI CA'TA_TG

Cuts at:      0     163    2049 
   Size:        163    1886
 
NsiI A_TGCA'T

Cuts at:      0     906    2049 
   Size:        906    1143
 
NspI r_CATG'y

Cuts at:      0     116     826    1089    2049 
   Size:        116     710     263     960

  Fragments arranged by size: 

                 960     710     263     116
 
PfoI T'CCnGG_A

Cuts at:      0    1424    2049 
   Size:       1424     625
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1361    1393    2049 
   Size:       1361      32     656

  Fragments arranged by size: 

                1361     656      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     550    2049 
   Size:        550    1499
 
PstI C_TGCA'G

Cuts at:      0    1683    2049 
   Size:       1683     366
 
PvuII CAG'CTG

Cuts at:      0      51    1242    1971    2049 
   Size:         51    1191     729      78

  Fragments arranged by size: 

                1191     729      78      51
 
SacI G_AGCT'C

Cuts at:      0    1985    2049 
   Size:       1985      64
 
SexAI A'CCwGG_T

Cuts at:      0     152    2049 
   Size:        152    1897
 
SfcI C'TryA_G

Cuts at:      0     102    1679    2049 
   Size:        102    1577     370

  Fragments arranged by size: 

                1577     370     102
 
SmlI C'TyrA_G

Cuts at:      0      71    1090    1656    2049 
   Size:         71    1019     566     393

  Fragments arranged by size: 

                1019     566     393      71
 
SphI G_CATG'C

Cuts at:      0     116    1089    2049 
   Size:        116     973     960

  Fragments arranged by size: 

                 973     960     116
 
StyI C'CwwG_G

Cuts at:      0      32     369    2049 
   Size:         32     337    1680

  Fragments arranged by size: 

                1680     337      32
 
TatI w'GTAC_w

Cuts at:      0     863    1620    2049 
   Size:        863     757     429

  Fragments arranged by size: 

                 863     757     429
 
Tth111I GACn'n_nGTC

Cuts at:      0    1960    2049 
   Size:       1960      89
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     480    2049 
   Size:        480    1569
 

 Enzymes that do cut:

     AarI     AcuI    AflII   AflIII     AlfI     AloI    AlwNI     ApoI
    BanII     BbsI    BbvCI    BfrBI    BglII     BlpI    BmgBI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI    BstXI    BstYI   Bsu36I     BtgI
     BtsI     ClaI    CspCI   DraIII     EarI  EcoICRI  Eco57MI EcoO109I
    EcoRI    Hin4I  HindIII     MmeI     MslI   MspA1I     NcoI     NdeI
     NsiI     NspI     PfoI     PpiI    PpuMI     PstI    PvuII     SacI
    SexAI     SfcI     SmlI     SphI     StyI     TatI  Tth111I     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI     AgeI     AhdI     AleI
     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI     BbeI     BcgI    BciVI     BclI     BglI Bme1580I
     BmrI     BmtI    BsaAI    BsaHI    BsiEI    BsiWI     BsmI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I    BtgZI     DraI     DrdI
     EaeI     EagI     EciI    EcoNI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MfeI     MluI
     MscI     NaeI     NarI   NgoMIV     NheI     NotI     NruI     PacI
     PasI     PciI    PflMI     PmeI     PmlI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SapI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SrfI     SspI     StuI     SwaI    TaqII     TstI     XbaI
     XhoI     XmaI     XmnI     ZraI
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