Restriction map for pF1KB3635
##################################
# NO CUT ENZYMES (  184 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      CelII       Cfr10I      
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HaeII       Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        Ksp632I     KspI        MaeII       MaeIII      
MamI        MfeI        MluI        MluNI       MscI        
MslI        MspA1I      MunI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tsp45I      Tsp509I     Tth111I     XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AccII         101  CGCG                 CG^CG
AccIII        185  TCCGGA               T^CCGGA
AlwNI         299  CAGATACTG            CAG[ACGT][ACGT][ACGT]^CTG
BamHI         323  GGATCC               G^GATCC
BfaI          158  CTAG                 C^TAG
BsaWI         185  TCCGGA               [AT]^CCGG[AT]
BseAI         185  TCCGGA               T^CCGGA
BseRI         478  GAGGAG               GAGGAG(10/8)
BsgI          213  CTGCAC               GTGCAG(16/14)
BspDI         337  ATCGAT               AT^CGAT
BspEI         185  TCCGGA               T^CCGGA
BsrI          304  ACTGG                ACTGG(1/-1)
BstUI         101  CGCG                 CG^CG
BstXI         110  CCAAGGTGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I         102  GCGAGC               GC[ACGT]^[ACGT]GC
ClaI          337  ATCGAT               AT^CGAT
DdeI          297  CTCAG                C^T[ACGT]AG
HapII         186  CCGG                 C^CGG
HgaI           99  GACGC                GACGC(5/10)
HinfI         467  GAATC                G^A[ACGT]TC
HpaII         186  CCGG                 C^CGG
HphI           16  GGTGA                GGTGA(8/7)
MaeI          158  CTAG                 C^TAG
MboII         238  GAAGA                GAAGA(8/7)
MroI          185  TCCGGA               T^CCGGA
MseI          280  TTAA                 T^TAA
MspI          186  CCGG                 C^CGG
MvnI          101  CGCG                 CG^CG
TfiI          467  GAATC                G^A[AT]TC
Tru9I         280  TTAA                 T^TAA


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AluI          125  AGCT                 AG^CT
              156  AGCT                
BsaJI         110  CCAAGG               C^C[ACGT][ACGT]GG
              230  CCAAGG              
BsmAI          89  GAGAC                GTCTC(1/5)
               62  GTCTC               
BstYI         172  AGATCC               [GA]^GATC[CT]
              323  GGATCC              
Cfr13I        227  GGGCC                G^G[ACGT]CC
              455  GGGCC               
EcoT14I       110  CCAAGG               C^C[AT][AT]GG
              230  CCAAGG              
FauI           36  GCGGG                CCCGC(4/6)
              267  GCGGG               
MflI          172  AGATCC               [GA]^GATC[CT]
              323  GGATCC              
NlaIII         33  CATG                 CATG^
              486  CATG                
PflMI         361  CCAAGCATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              485  CCATGCGCTGG         
Sau96I        227  GGGCC                G^G[ACGT]CC
              455  GGGCC               
StyI          110  CCAAGG               C^C[AT][AT]GG
              230  CCAAGG              
TaqI          222  TCGA                 T^CGA
              338  TCGA                
TspRI         165  CACTG                CA[GC]TG[ACGT][ACGT]^
              211  CACTG               
TthHB8I       222  TCGA                 T^CGA
              338  TCGA                
Van91I        361  CCAAGCATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              485  CCATGCGCTGG         
XhoII         172  AGATCC               [GA]^GATC[CT]
              323  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   27 enzymes) #
#########################################

AciI        AlwI        BbvI        BsiYI       BslI        
BssKI       BstF5I      BstNI       CfoI        DpnII       
EcoRII      Fnu4HI      FokI        HaeIII      HhaI        
HinP1I      ItaI        MboI        MnlI        MvaI        
MwoI        NdeII       NlaIV       Sau3AI      ScrFI       
SfaNI       TseI        
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