Restriction map for pF1KB3483
##################################
# NO CUT ENZYMES (  148 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Aor51HI     ApaI        ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AviII       AvrII       BamHI       BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaHI       
BsaWI       BseAI       BsgI        BsiEI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstYI       BstZ17I     CelII       
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoRI       EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MaeI        MfeI        MflI        
MluI        MroI        MunI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Tsp509I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

AccII         622  CGCG                 CG^CG
AluI          351  AGCT                 AG^CT
Alw44I        526  GTGCAC               G^TGCAC
AlwNI         347  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          151  CCTCAGG              CC^T[ACGT]AGG
ApaLI         526  GTGCAC               G^TGCAC
AspHI         526  GTGCAC               G[AT]GC[AT]^C
AspI          532  GACTGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          114  CCCGGG               C^[CT]CG[GA]G
BalI           38  TGGCCA               TGG^CCA
BanI          280  GGCACC               G^G[CT][GA]CC
BmyI          526  GTGCAC               G[AGT]GC[ACT]^C
BsaBI         636  GATGTGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          598  GAGACC               GGTCTC(1/5)
BseRI         705  CTCCTC               GAGGAG(10/8)
BsiHKAI       526  GTGCAC               G[AT]GC[AT]^C
BsmAI         598  GAGAC                GTCTC(1/5)
BsmFI         243  GGGAC                GGGAC(10/14)
BsoBI         114  CCCGGG               C^[CT]CG[GA]G
Bsp1286I      526  GTGCAC               G[AGT]GC[ACT]^C
BstEII        564  GGTCACC              G^GT[ACGT]ACC
BstPI         564  GGTCACC              G^GT[ACGT]ACC
BstUI         622  CGCG                 CG^CG
BstXI         228  CCATGGTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        151  CCTCAGG              CC^T[ACGT]AGG
Cac8I         404  GCAGGC               GC[ACGT]^[ACGT]GC
DraII         368  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EaeI           38  TGGCCA               [CT]^GGCC[GA]
EarI          700  CTCTTC               CTCTTC(1/4)
Eco81I        151  CCTCAGG              CC^T[ACGT]AGG
EcoO109I      368  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       564  GGTCACC              G^GT[ACGT]ACC
HaeII          71  AGCGCC               [GA]GCGC^[CT]
HapII         115  CCGG                 C^CGG
HgaI          536  GCGTC                GACGC(5/10)
HinfI         661  GAATC                G^A[ACGT]TC
HpaII         115  CCGG                 C^CGG
Ksp632I       700  CTCTTC               CTCTTC(1/4)
MaeII         605  ACGT                 A^CGT
MamI          636  GATGTGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI          38  TGGCCA               TGG^CCA
MscI           38  TGGCCA               TGG^CCA
MseI          608  TTAA                 T^TAA
MspA1I        350  CAGCTG               C[AC]G^C[GT]G
MspI          115  CCGG                 C^CGG
MvnI          622  CGCG                 CG^CG
PflFI         532  GACTGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PstI          344  CTGCAG               CTGCA^G
PvuII         350  CAGCTG               CAG^CTG
SfaNI          21  GATGC                GCATC(5/9)
SmaI          114  CCCGGG               CCC^GGG
StuI          668  AGGCCT               AGG^CCT
TaqI          688  TCGA                 T^CGA
TfiI          661  GAATC                G^A[AT]TC
Tru9I         608  TTAA                 T^TAA
Tth111I       532  GACTGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       688  TCGA                 T^CGA
XmaCI         114  CCCGGG               C^CCGGG
XmaI          114  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AlwI          720  GATCC                GGATC(4/5)
              685  GGATC               
AvaII         438  GGACC                G^G[AT]CC
              696  GGTCC               
BcnI          114  CCCGG                CC^[GC]GG
              115  CCGGG               
BglI          128  GCCCTGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              371  GCCTTGGCGGC         
BpmI           83  CTGGAG               CTGGAG(16/14)
              131  CTGGAG              
BsrI           97  ACTGG                ACTGG(1/-1)
              648  CCAGT               
CfoI           72  GCGC                 GCG^C
              621  GCGC                
DdeI          152  CTCAG                C^T[ACGT]AG
              665  CTCAG               
DsaI          228  CCATGG               C^C[GA][CT]GG
              494  CCATGG              
HhaI           72  GCGC                 GCG^C
              621  GCGC                
HinP1I         72  GCGC                 G^CGC
              621  GCGC                
MboII         701  TCTTC                GAAGA(8/7)
              743  TCTTC               
NciI          114  CCCGG                CC^[GC]GG
              115  CCGGG               
NcoI          228  CCATGG               C^CATGG
              494  CCATGG              
NlaIV         280  GGCACC               GG[ACGT]^[ACGT]CC
              368  GGGGCC              
NspI          383  ACATGC               [GA]CATG^[CT]
              458  ACATGC              
SfcI          344  CTGCAG               C^T[GA][CT]AG
              486  CTACAG              


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AciI        AfaI        BbvI        BsaJI       BsiYI       
BslI        BssKI       BstF5I      BstNI       Cfr13I      
DpnII       EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HphI        ItaI        MaeIII      MboI        
MnlI        MslI        MvaI        MwoI        NdeII       
NlaIII      RsaI        Sau3AI      Sau96I      ScrFI       
StyI        TseI        Tsp45I      TspRI       
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