Restriction map for pF1KB3483
 (Linear) (Six-base) MAPSORT of: KIBB3483.seq  Check: 1311  from: 1  to: 759



KIBB3483.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:56  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     352     759 
   Size:        352     407
 
ApaLI G'TGCA_C

Cuts at:      0     526     759 
   Size:        526     233
 
AvaI C'yCGr_G

Cuts at:      0     114     759 
   Size:        114     645
 
BanI G'GyrC_C

Cuts at:      0     280     759 
   Size:        280     479
 
BglI GCCn_nnn'nGGC

Cuts at:      0     134     377     759 
   Size:        134     243     382

  Fragments arranged by size: 

                 382     243     134
 
Bme1580I G_kGCm'C

Cuts at:      0     530     759 
   Size:        530     229
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     107     642     759 
   Size:        107     535     117

  Fragments arranged by size: 

                 535     117     107
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     680     759 
   Size:        680      79
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     104     152     759 
   Size:        104      48     607

  Fragments arranged by size: 

                 607     104      48
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     592     759 
   Size:        592     167
 
BsaBI GATnn'nnATC

Cuts at:      0     640     759 
   Size:        640     119
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     696     759 
   Size:        696      63
 
BseYI C'CCAG_C

Cuts at:      0     167     191     215     239     263     352     387     759 
   Size:        167      24      24      24      24      89      35     372

  Fragments arranged by size: 

                 372     167      89      35      24      24      24      24
            
 
BsiHKAI G_wGCw'C

Cuts at:      0     530     759 
   Size:        530     229
 
Bsp1286I G_dGCh'C

Cuts at:      0     530     759 
   Size:        530     229
 
BstEII G'GTnAC_C

Cuts at:      0     564     759 
   Size:        564     195
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     235     759 
   Size:        235     524
 
Bsu36I CC'TnA_GG

Cuts at:      0     152     759 
   Size:        152     607
 
BtgI C'CryG_G

Cuts at:      0     228     494     759 
   Size:        228     266     265

  Fragments arranged by size: 

                 266     265     228
 
BtsI GCAGTG_nn'

Cuts at:      0     365     432     759 
   Size:        365      67     327

  Fragments arranged by size: 

                 365     327      67
 
EaeI y'GGCC_r

Cuts at:      0      38     759 
   Size:         38     721
 
EarI CTCTTCn'nnn_

Cuts at:      0     706     759 
   Size:        706      53
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     104     152     759 
   Size:        104      48     607

  Fragments arranged by size: 

                 607     104      48
 
EcoO109I rG'GnC_Cy

Cuts at:      0     369     759 
   Size:        369     390
 
HaeII r_GCGC'y

Cuts at:      0      75     759 
   Size:         75     684
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     646     678     759 
   Size:        646      32      81

  Fragments arranged by size: 

                 646      81      32
 
MscI TGG'CCA

Cuts at:      0      40     759 
   Size:         40     719
 
MslI CAynn'nnrTG

Cuts at:      0     185     209     233     257     759 
   Size:        185      24      24      24     502

  Fragments arranged by size: 

                 502     185      24      24      24
 
MspA1I CmG'CkG

Cuts at:      0     352     759 
   Size:        352     407
 
NcoI C'CATG_G

Cuts at:      0     228     494     759 
   Size:        228     266     265

  Fragments arranged by size: 

                 266     265     228
 
NspI r_CATG'y

Cuts at:      0     387     462     759 
   Size:        387      75     297

  Fragments arranged by size: 

                 387     297      75
 
PstI C_TGCA'G

Cuts at:      0     348     759 
   Size:        348     411
 
PvuII CAG'CTG

Cuts at:      0     352     759 
   Size:        352     407
 
SfcI C'TryA_G

Cuts at:      0     344     486     759 
   Size:        344     142     273

  Fragments arranged by size: 

                 344     273     142
 
SmaI CCC'GGG

Cuts at:      0     116     759 
   Size:        116     643
 
StuI AGG'CCT

Cuts at:      0     670     759 
   Size:        670      89
 
StyI C'CwwG_G

Cuts at:      0       9     228     372     494     578     759 
   Size:          9     219     144     122      84     181

  Fragments arranged by size: 

                 219     181     144     122      84       9
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     298     616     661     759 
   Size:        298     318      45      98

  Fragments arranged by size: 

                 318     298      98      45
 
TatI w'GTAC_w

Cuts at:      0     482     503     759 
   Size:        482      21     256

  Fragments arranged by size: 

                 482     256      21
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     704     736     759 
   Size:        704      32      23

  Fragments arranged by size: 

                 704      32      23
 
Tth111I GACn'n_nGTC

Cuts at:      0     535     759 
   Size:        535     224
 
XmaI C'CCGG_G

Cuts at:      0     114     759 
   Size:        114     645
 

 Enzymes that do cut:

    AlwNI    ApaLI     AvaI     BanI     BglI Bme1580I     BmrI     BplI
     BpmI     BsaI    BsaBI    BseRI    BseYI  BsiHKAI Bsp1286I   BstEII
    BstXI   Bsu36I     BtgI     BtsI     EaeI     EarI  Eco57MI EcoO109I
    HaeII    Hin4I     MscI     MslI   MspA1I     NcoI     NspI     PstI
    PvuII     SfcI     SmaI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
    BmgBI     BmtI   Bpu10I    BpuEI    BsaAI    BsaHI    BsaWI    BsaXI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
    BstYI  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI     XbaI     XcmI     XhoI
     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com