Restriction map for
pF1KB3427
(Circular) (Six-base) MAPSORT of: pF1KB3427.seq Check: 6641 from: 1 to: 4388
pF1KB3427 4388 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 561 1426 561
Size: 865 3523
Acc65I G'GTAC_C
Cuts at: 706 1404 706
Size: 698 3690
AclI AA'CG_TT
Cuts at: 354 618 1014 4228 354
Size: 264 396 3214 514
Fragments arranged by size:
3214 514 396 264
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1205 2213 2645 2998 3660 1205
Size: 1008 432 353 662 1933
Fragments arranged by size:
1933 1008 662 432 353
AflIII A'CryG_T
Cuts at: 1740 3540 3821 1740
Size: 1800 281 2307
Fragments arranged by size:
2307 1800 281
AleI CACnn'nnGTG
Cuts at: 431 431
Size: 4388
AlwNI CAG_nnn'CTG
Cuts at: 1075 1693 3131 1075
Size: 618 1438 2332
Fragments arranged by size:
2332 1438 618
ApaLI G'TGCA_C
Cuts at: 382 892 3226 382
Size: 510 2334 1544
Fragments arranged by size:
2334 1544 510
ApoI r'AATT_y
Cuts at: 211 1392 3741 3997 211
Size: 1181 2349 256 602
Fragments arranged by size:
2349 1181 602 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4388
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4388
AvaI C'yCGr_G
Cuts at: 1408 1408
Size: 4388
BamHI G'GATC_C
Cuts at: 1413 1413
Size: 4388
BanI G'GyrC_C
Cuts at: 706 1404 1818 2066 2101 706
Size: 698 414 248 35 2993
Fragments arranged by size:
2993 698 414 248 35
BanII G_rGCy'C
Cuts at: 1402 2432 1402
Size: 1030 3358
BbeI G_GCGC'C
Cuts at: 1822 2070 1822
Size: 248 4140
BbvCI CC'TCA_GC
Cuts at: 341 341
Size: 4388
BciVI GTATCCnnnnn_n'
Cuts at: 1337 2286 3342 4388 1337
Size: 949 1056 1046 1337
Fragments arranged by size:
1337 1056 1046 949
BclI T'GATC_A
Cuts at: 1908 1908
Size: 4388
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4388
BglII A'GATC_T
Cuts at: 1903 3940 1903
Size: 2037 2351
BlpI GC'TnA_GC
Cuts at: 1500 1500
Size: 4388
Bme1580I G_kGCm'C
Cuts at: 386 896 2013 2106 3230 386
Size: 510 1117 93 1124 1544
Fragments arranged by size:
1544 1124 1117 510 93
BmrI ACTGGGnnnn_n'
Cuts at: 1600 1681 2016 4160 1600
Size: 81 335 2144 1828
Fragments arranged by size:
2144 1828 335 81
BmtI G_CTAG'C
Cuts at: 878 878
Size: 4388
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2755 2755
Size: 4388
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 334 640 1304 137
Size: 197 306 664 3221
Fragments arranged by size:
3221 664 306 197
Bpu10I CC'TnA_GC
Cuts at: 341 545 341
Size: 204 4184
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 916 957 1564 2443 3157 3455 916
Size: 41 607 879 714 298 1849
Fragments arranged by size:
1849 879 714 607 298 41
BsaI GGTCTCn'nnnn_
Cuts at: 4092 4092
Size: 4388
BsaAI yAC'GTr
Cuts at: 381 1741 381
Size: 1360 3028
BsaBI GATnn'nnATC
Cuts at: 6 1902 3939 6
Size: 1896 2037 455
Fragments arranged by size:
2037 1896 455
BsaHI Gr'CG_yC
Cuts at: 1819 2067 2769 4265 1819
Size: 248 702 1496 1942
Fragments arranged by size:
1942 1496 702 248
BsaWI w'CCGG_w
Cuts at: 215 1577 2098 2776 3187 3334 3608 215
Size: 1362 521 678 411 147 274 995
Fragments arranged by size:
1362 995 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1333 1333
Size: 4388
BseYI C'CCAG_C
Cuts at: 1813 2571 3236 3644 1813
Size: 758 665 408 2557
Fragments arranged by size:
2557 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 578 1321 578
Size: 743 3645
BsiEI CG_ry'CG
Cuts at: 104 1930 1976 3206 104
Size: 1826 46 1230 1286
Fragments arranged by size:
1826 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 386 896 1402 2180 2370 3230 386
Size: 510 506 778 190 860 1544
Fragments arranged by size:
1544 860 778 510 506 190
BsmI GAATG_Cn'
Cuts at: 1223 1223
Size: 4388
Bsp1286I G_dGCh'C
Cuts at: 386 896 1402 2013 2106 2180 2370 2432 3230
Size: 510 506 611 93 74 190 62 798
Cuts at: 3230 386
Size: 1544
Fragments arranged by size:
1544 798 611 510 506 190 93 74
62
BspEI T'CCGG_A
Cuts at: 215 215
Size: 4388
BspHI T'CATG_A
Cuts at: 2820 2820
Size: 4388
BspMI ACCTGCnnnn'nnnn_
Cuts at: 503 692 1438 1652 1954 2335 503
Size: 189 746 214 302 381 2556
Fragments arranged by size:
2556 746 381 302 214 189
BsrBI CCG'CTC
Cuts at: 50 149 2680 2734 4218 4383 50
Size: 99 2531 54 1484 165 55
Fragments arranged by size:
2531 1484 165 99 55 54
BsrDI GCAATG_nn'
Cuts at: 2300 2300
Size: 4388
BsrFI r'CCGG_y
Cuts at: 2386 2567 2386
Size: 181 4207
BssSI C'ACGA_G
Cuts at: 2659 3367 2659
Size: 708 3680
BstAPI GCAn_nnn'nTGC
Cuts at: 1236 1765 1236
Size: 529 3859
BstBI TT'CG_AA
Cuts at: 2749 2749
Size: 4388
BstYI r'GATC_y
Cuts at: 1 639 651 1121 1413 1880 1903 2238 2484
Size: 638 12 470 292 467 23 335 246
Cuts at: 2484 2790 2802 2888 3940 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 638 470 467 449 335 306 292
246 86 23 12 12
BtgI C'CryG_G
Cuts at: 107 1271 107
Size: 1164 3224
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 994 2338 2519 2682 994
Size: 1344 181 163 2700
Fragments arranged by size:
2700 1344 181 163
BtsI GCAGTG_nn'
Cuts at: 396 1631 396
Size: 1235 3153
DraI TTT'AAA
Cuts at: 1387 1387
Size: 4388
DrdI GACnn_nn'nnGTC
Cuts at: 2094 3438 2094
Size: 1344 3044
EaeI y'GGCC_r
Cuts at: 393 1130 1247 1268 1973 2147 2538 2565 3910
Size: 737 117 21 705 174 391 27 1345
Cuts at: 3910 393
Size: 871
Fragments arranged by size:
1345 871 737 705 391 174 117 27
21
EagI C'GGCC_G
Cuts at: 1973 1973
Size: 4388
EarI CTCTTCn'nnn_
Cuts at: 341 1310 2411 2621 341
Size: 969 1101 210 2108
Fragments arranged by size:
2108 1101 969 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 262 2596 3338 3484 4198 262
Size: 2334 742 146 714 452
Fragments arranged by size:
2334 742 714 452 146
EcoICRI GAG'CTC
Cuts at: 1400 1400
Size: 4388
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 334 640 1205 1304 2213 2645 2998 3660
Size: 197 306 565 99 909 432 353 662
Cuts at: 3660 137
Size: 865
Fragments arranged by size:
909 865 662 565 432 353 306 197
99
EcoO109I rG'GnC_Cy
Cuts at: 1527 1527
Size: 4388
EcoRI G'AATT_C
Cuts at: 1392 1392
Size: 4388
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2407 2407
Size: 4388
FspI TGC'GCA
Cuts at: 2169 2169
Size: 4388
HaeII r_GCGC'y
Cuts at: 1822 2070 3300 4071 1822
Size: 248 1230 771 2139
Fragments arranged by size:
2139 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 289 321 624 656 800 832 2230 2260 2262
Size: 32 303 32 144 32 1398 30 2
Cuts at: 2262 2292 289
Size: 30 2385
Fragments arranged by size:
2385 1398 303 144 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1427 1427
Size: 4388
HindIII A'AGCT_T
Cuts at: 508 508
Size: 4388
KasI G'GCGC_C
Cuts at: 1818 2066 1818
Size: 248 4140
KpnI G_GTAC'C
Cuts at: 710 1408 710
Size: 698 3690
MluI A'CGCG_T
Cuts at: 3821 3821
Size: 4388
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 432 3147 3331 432
Size: 2715 184 1489
Fragments arranged by size:
2715 1489 184
MscI TGG'CCA
Cuts at: 395 1249 1270 2149 395
Size: 854 21 879 2634
Fragments arranged by size:
2634 879 854 21
MslI CAynn'nnrTG
Cuts at: 431 1264 1270 2377 2504 431
Size: 833 6 1107 127 2315
Fragments arranged by size:
2315 1107 833 127 6
MspA1I CmG'CkG
Cuts at: 231 340 600 1499 1813 2173 2957 3202 231
Size: 109 260 899 314 360 784 245 1417
Fragments arranged by size:
1417 899 784 360 314 260 245 109
NaeI GCC'GGC
Cuts at: 2569 2569
Size: 4388
NarI GG'CG_CC
Cuts at: 1819 2067 1819
Size: 248 4140
NcoI C'CATG_G
Cuts at: 107 1271 107
Size: 1164 3224
NdeI CA'TA_TG
Cuts at: 1344 1344
Size: 4388
NgoMIV G'CCGG_C
Cuts at: 2567 2567
Size: 4388
NheI G'CTAG_C
Cuts at: 874 874
Size: 4388
NspI r_CATG'y
Cuts at: 1441 1941 3544 1441
Size: 500 1603 2285
Fragments arranged by size:
2285 1603 500
PciI A'CATG_T
Cuts at: 3540 3540
Size: 4388
PfoI T'CCnGG_A
Cuts at: 293 293
Size: 4388
PmeI GTTT'AAAC
Cuts at: 1387 1387
Size: 4388
PmlI CAC'GTG
Cuts at: 381 381
Size: 4388
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2834 2866 2834
Size: 32 4356
PshAI GACnn'nnGTC
Cuts at: 1048 1048
Size: 4388
PstI C_TGCA'G
Cuts at: 417 737 1024 1175 1435 2120 417
Size: 320 287 151 260 685 2685
Fragments arranged by size:
2685 685 320 287 260 151
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4388
PvuII CAG'CTG
Cuts at: 231 340 600 1813 2173 231
Size: 109 260 1213 360 2446
Fragments arranged by size:
2446 1213 360 260 109
RsrII CG'GwC_CG
Cuts at: 2583 2583
Size: 4388
SacI G_AGCT'C
Cuts at: 1402 1402
Size: 4388
SalI G'TCGA_C
Cuts at: 1425 1425
Size: 4388
SapI GCTCTTCn'nnn_
Cuts at: 2411 2621 2411
Size: 210 4178
SbfI CC_TGCA'GG
Cuts at: 1435 1435
Size: 4388
ScaI AGT'ACT
Cuts at: 860 3905 860
Size: 3045 1343
SfcI C'TryA_G
Cuts at: 33 413 733 1020 1063 1171 1431 2116 3084
Size: 380 320 287 43 108 260 685 968
Cuts at: 3084 3275 33
Size: 191 1146
Fragments arranged by size:
1146 968 685 380 320 287 260 191
108 43
SfoI GGC'GCC
Cuts at: 1820 2068 1820
Size: 248 4140
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4388
SmaI CCC'GGG
Cuts at: 1410 1410
Size: 4388
SmlI C'TyrA_G
Cuts at: 931 972 1543 2458 3172 3434 931
Size: 41 571 915 714 262 1885
Fragments arranged by size:
1885 915 714 571 262 41
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4388
SphI G_CATG'C
Cuts at: 1441 1941 1441
Size: 500 3888
StyI C'CwwG_G
Cuts at: 107 429 1271 1522 1876 107
Size: 322 842 251 354 2619
Fragments arranged by size:
2619 842 354 322 251
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1971 2104 2563 2772 1971
Size: 133 459 209 3587
Fragments arranged by size:
3587 459 209 133
TatI w'GTAC_w
Cuts at: 858 3903 858
Size: 3045 1343
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1181 1213 3905 3937 1181
Size: 32 2692 32 1632
Fragments arranged by size:
2692 1632 32 32
Tth111I GACn'n_nGTC
Cuts at: 2185 2185
Size: 4388
XbaI T'CTAG_A
Cuts at: 1419 1419
Size: 4388
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1883 1883
Size: 4388
XmaI C'CCGG_G
Cuts at: 1408 1408
Size: 4388
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NspI PciI PfoI PmeI PmlI
PpiI PshAI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
ApaI AscI AvrII BaeI BbsI BcgI BfrBI BmgBI
BsaXI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NotI NruI NsiI PacI PasI PflMI
PpuMI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI