Restriction map for pF1KB3422
##################################
# NO CUT ENZYMES (  189 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfrI        BglI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsiEI       BsiWI       BsmAI       
BsmBI       BsmFI       BsmI        Bsp1407I    BspDI       
BspEI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BsrI        BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      ClaI        
CpoI        DdeI        DpnII       DraI        DraII       
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HphI        KasI        KpnI        KspI        
MamI        MboI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MunI        MvnI        
NaeI        NarI        NcoI        NdeI        NdeII       
NgoMIV      NheI        NlaIV       NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RsrII       SacII       SalI        
Sau3AI      Sau96I      SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TaqI        TfiI        
Tsp509I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AfaI          211  GTAC                 GT^AC
Asp700I       253  GAAACTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI            7  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI           94  CCCGAG               C^[CT]CG[GA]G
BcnI           72  CCGGG                CC^[GC]GG
BseRI         346  GAGGAG               GAGGAG(10/8)
BsgI          239  CTGCAC               GTGCAG(16/14)
BsoBI          94  CCCGAG               C^[CT]CG[GA]G
BspHI          80  TCATGA               T^CATGA
BstAPI        232  GCAAACACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I         69  CATCC                GGATG(2/0)
BstNI         114  CCAGG                CC^[AT]GG
DsaI          143  CCACGG               C^C[GA][CT]GG
EaeI          134  CGGCCA               [CT]^GGCC[GA]
EarI           84  GAAGAG               CTCTTC(1/4)
EcoRII        114  CCAGG                ^CC[AT]GG
EcoT14I       137  CCAAGG               C^C[AT][AT]GG
FokI           69  CATCC                GGATG(9/13)
HapII          72  CCGG                 C^CGG
HinfI         388  GACTC                G^A[ACGT]TC
HpaII          72  CCGG                 C^CGG
Ksp632I        84  GAAGAG               CTCTTC(1/4)
MaeII           8  ACGT                 A^CGT
MaeIII        371  GTGAC                ^GT[ACGT]AC
MseI          320  TTAA                 T^TAA
MslI          187  CACGGCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I         40  CAGCGG               C[AC]G^C[GT]G
MspI           72  CCGG                 C^CGG
MvaI          114  CCAGG                CC^[AT]GG
NciI           72  CCGGG                CC^[GC]GG
PflFI           7  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          388  GACTC                GAGTC(4/5)
RcaI           80  TCATGA               T^CATGA
RsaI          211  GTAC                 GT^AC
SapI           84  GAAGAGC              GCTCTTC(1/4)
ScaI          210  AGTACT               AGT^ACT
SfcI          180  CTACAG               C^T[GA][CT]AG
StyI          137  CCAAGG               C^C[AT][AT]GG
Tru9I         320  TTAA                 T^TAA
Tsp45I        371  GTGAC                ^GT[GC]AC
Tth111I         7  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmnI          253  GAAACTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

BanII          87  GAGCTC               G[GA]GC[CT]^C
              148  GAGCTC              
BbvI           39  GCAGC                GCAGC(8/12)
               54  GCTGC               
BfaI          166  CTAG                 C^TAG
              299  CTAG                
BsiYI          65  CCCGCATCCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               66  CCGCATCCGGG         
BslI           65  CCCGCATCCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               66  CCGCATCCGGG         
BssKI          72  CCGGG                ^CC[ACGT]GG
              114  CCAGG               
FauI           65  CCCGC                CCCGC(4/6)
               32  GCGGG               
HaeIII        135  GGCC                 GG^CC
              141  GGCC                
MaeI          166  CTAG                 C^TAG
              299  CTAG                
MwoI           45  GCTCATCTCGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              232  GCAAACACTGC         
NlaIII         81  CATG                 CATG^
              376  CATG                
SacI           87  GAGCTC               GAGCT^C
              148  GAGCTC              
ScrFI          72  CCGGG                CC^[ACGT]GG
              114  CCAGG               
SfaNI          68  GCATC                GCATC(5/9)
              107  GCATC               
TseI           39  GCAGC                G^C[AT]GC
               54  GCTGC               


#########################################
# 3 OR MORE CUT ENZYMES (   12 enzymes) #
#########################################

AciI        AluI        AspHI       BmyI        BsaJI       
BsiHKAI     Bsp1286I    Fnu4HI      ItaI        MboII       
MnlI        TspRI       
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