Restriction map for pF1KB3406
##################################
# NO CUT ENZYMES (  156 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BamHI       BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BglI        BlnI        
BmyI        BpuAI       BsaBI       BsaHI       BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstZ17I     Bsu36I      Cac8I       CfoI        
Cfr10I      ClaI        CpoI        DraII       DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HpaI        HpaII       KasI        
KpnI        KspI        MamI        MfeI        MluI        
MroI        MspI        MunI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SacI        
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SphI        Sse8387I    SspBI       SspI        StuI        
Tth111I     XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccII          24  CGCG                 CG^CG
AfaI          802  GTAC                 GT^AC
AflII          91  CTTAAG               C^TTAAG
AlwNI         340  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AseI          565  ATTAAT               AT^TAAT
AsnI          565  ATTAAT               AT^TAAT
BalI          176  TGGCCA               TGG^CCA
BfaI          871  CTAG                 C^TAG
BfrI           91  CTTAAG               C^TTAAG
BglII         851  AGATCT               A^GATCT
BlpI          106  GCTAAGC              GC^T[ACGT]AGC
BpmI          939  CTCCAG               CTGGAG(16/14)
Bpu1102I      106  GCTAAGC              GC^T[ACGT]AGC
BsaAI         662  TACGTA               [CT]AC^GT[GA]
BsaI         1018  GGTCTC               GGTCTC(1/5)
BsmAI        1019  GTCTC                GTCTC(1/5)
BsrDI         405  CATTGC               GCAATG(2/0)
BstUI          24  CGCG                 CG^CG
CelII         106  GCTAAGC              GC^T[ACGT]AGC
DraI          997  TTTAAA               TTT^AAA
EaeI          176  TGGCCA               [CT]^GGCC[GA]
EarI          615  GAAGAG               CTCTTC(1/4)
EcoRI         283  GAATTC               G^AATTC
EcoRV         775  GATATC               GAT^ATC
EcoT14I       374  CCAAGG               C^C[AT][AT]GG
HincII        231  GTCAAC               GT[CT]^[GA]AC
HindII        231  GTCAAC               GT[CT]^[GA]AC
HindIII       109  AAGCTT               A^AGCTT
Ksp632I       615  GAAGAG               CTCTTC(1/4)
MaeI          871  CTAG                 C^TAG
MluNI         176  TGGCCA               TGG^CCA
MscI          176  TGGCCA               TGG^CCA
MvnI           24  CGCG                 CG^CG
NlaIII        966  CATG                 CATG^
PshBI         565  ATTAAT               AT^TAAT
PvuII         677  CAGCTG               CAG^CTG
RsaI          802  GTAC                 GT^AC
SapI          615  GAAGAGC              GCTCTTC(1/4)
SfcI          674  CTACAG               C^T[GA][CT]AG
SmlI           91  CTTAAG               C^T[CT][GA]AG
SnaBI         662  TACGTA               TAC^GTA
SpeI          870  ACTAGT               A^CTAGT
StyI          374  CCAAGG               C^C[AT][AT]GG
SwaI          996  ATTTAAAT             ATTT^AAAT
Tsp45I        655  GTGAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AciI          822  CCGC                 CCGC(-3/-1)
               25  GCGG                
AlwI          336  GATCC                GGATC(4/5)
              348  GGATC               
AvaII         154  GGTCC                G^G[AT]CC
              944  GGACC               
BsaJI         354  CCCAGG               C^C[ACGT][ACGT]GG
              374  CCAAGG              
BsmFI         465  GGGAC                GGGAC(10/14)
              857  GGGAC               
BsmI          506  GCATTC               GAATGC(1/-1)
              620  GCATTC              
BsrI          900  CCAGT                ACTGG(1/-1)
              922  CCAGT               
HphI          187  GGTGA                GGTGA(8/7)
              819  TCACC               
MaeII         663  ACGT                 A^CGT
             1063  ACGT                
MslI          405  CATTGCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              690  CATTGATGTG          
MspA1I        677  CAGCTG               C[AC]G^C[GT]G
              822  CCGCTG              
PleI           51  GACTC                GAGTC(4/5)
              657  GACTC               
TaqI          117  TCGA                 T^CGA
              195  TCGA                
TfiI          639  GATTC                G^A[AT]TC
              972  GATTC               
TthHB8I       117  TCGA                 T^CGA
              195  TCGA                
XcmI          761  CCATTGAGGAATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              941  CCAGGACCACAGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   41 enzymes) #
#########################################

AcsI        AluI        ApoI        BanI        BbvI        
BsiYI       BslI        BssKI       BstF5I      BstNI       
BstYI       Cfr13I      DdeI        DpnII       EcoRII      
Fnu4HI      FokI        HaeIII      HinfI       ItaI        
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MvaI        MwoI        NdeII       NlaIV       
PflMI       Sau3AI      Sau96I      ScrFI       SfaNI       
Tru9I       TseI        Tsp509I     TspRI       Van91I      
XhoII       
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