Restriction map for pF1KB3111
##################################
# NO CUT ENZYMES (  134 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AflIII      AlwNI       Aor51HI     ApaI        
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspI        AvaI        AviII       AvrII       
BalI        BamHI       BbrPI       BbsI        BbvCI       
BciVI       BfrI        BglI        BlnI        BlpI        
Bpu1102I    BpuAI       BsaBI       BsaI        BseAI       
BsiEI       BsiWI       BsoBI       Bsp1407I    BspDI       
BspEI       BspLU11I    BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstXI       BstZ17I     
CelII       ClaI        CpoI        DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
EclXI       Eco47III    Eco52I      EcoNI       EcoO109I    
EcoRI       EcoRV       EcoT14I     FseI        FspI        
HincII      HindII      HindIII     HpaI        KpnI        
KspI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MunI        NaeI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfuI        SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StyI        
SwaI        TaqI        Tth111I     TthHB8I     Van91I      
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccI          281  GTAGAC               GT^[AC][GT]AC
AccII         118  CGCG                 CG^CG
AciI          429  GCGG                 CCGC(-3/-1)
AgeI            7  ACCGGT               A^CCGGT
AhdI          284  GACCCCCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        131  GTGCAC               G^TGCAC
AocI          297  CCTGAGG              CC^T[ACGT]AGG
ApaLI         131  GTGCAC               G^TGCAC
AspEI         284  GACCCCCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         131  GTGCAC               G[AT]GC[AT]^C
BanI          907  GGCGCC               G^G[CT][GA]CC
BanII         383  GGGCTC               G[GA]GC[CT]^C
BbeI          907  GGCGCC               GGCGC^C
BcgI           77  GCAGTATCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          221  TGATCA               T^GATCA
BcnI          288  CCCGG                CC^[GC]GG
BglII         482  AGATCT               A^GATCT
BsaAI         134  CACGTA               [CT]AC^GT[GA]
BseRI         121  GAGGAG               GAGGAG(10/8)
BsgI          724  CTGCAC               GTGCAG(16/14)
BsiHKAI       131  GTGCAC               G[AT]GC[AT]^C
BsmBI        1060  GAGACG               CGTCTC(1/5)
BsmI          449  GCATTC               GAATGC(1/-1)
BspHI         855  TCATGA               T^CATGA
BsrBI         427  GAGCGG               CCGCTC(-3/-3)
BsrDI         736  GCAATG               GCAATG(2/0)
BstUI         118  CGCG                 CG^CG
Bsu36I        297  CCTGAGG              CC^T[ACGT]AGG
Eam1105I      284  GACCCCCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          238  GAAGAG               CTCTTC(1/4)
Eco81I        297  CCTGAGG              CC^T[ACGT]AGG
FauI          429  GCGGG                CCCGC(4/6)
FbaI          221  TGATCA               T^GATCA
HaeII         907  GGCGCC               [GA]GCGC^[CT]
KasI          907  GGCGCC               G^GCGCC
Ksp632I       238  GAAGAG               CTCTTC(1/4)
MaeII         135  ACGT                 A^CGT
MslI            6  CACCGGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        272  CAGCTG               C[AC]G^C[GT]G
MvnI          118  CGCG                 CG^CG
NarI          907  GGCGCC               GG^CGCC
NciI          288  CCCGG                CC^[GC]GG
PinAI           7  ACCGGT               A^CCGGT
PleI          502  GAGTC                GAGTC(4/5)
PvuII         272  CAGCTG               CAG^CTG
RcaI          855  TCATGA               T^CATGA
SfoI          907  GGCGCC               GGC^GCC
SgrAI           6  CACCGGTG             C[GA]^CCGG[CT]G
StuI          671  AGGCCT               AGG^CCT
XbaI          666  TCTAGA               T^CTAGA
XcmI         1013  CCAGCATTAGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AcyI          899  GGCGTC               G[GA]^CG[CT]C
              907  GGCGCC              
BfaI          317  CTAG                 C^TAG
              667  CTAG                
BmyI          131  GTGCAC               G[AGT]GC[ACT]^C
              383  GGGCTC              
BpmI          803  CTCCAG               CTGGAG(16/14)
              652  CTGGAG              
BsaHI         899  GGCGTC               G[GA]^CG[CT]C
              907  GGCGCC              
BsaJI         287  CCCCGG               C^C[ACGT][ACGT]GG
             1055  CCTCGG              
BsmAI        1060  GAGAC                GTCTC(1/5)
              747  GTCTC               
Bsp1286I      131  GTGCAC               G[AGT]GC[ACT]^C
              383  GGGCTC              
BspMI         568  GCAGGT               ACCTGC(4/8)
              987  GCAGGT              
BsrFI           7  ACCGGT               [GA]^CCGG[CT]
              533  ACCGGC              
BstEII        291  GGTCACC              G^GT[ACGT]ACC
              990  GGTTACC             
BstPI         291  GGTCACC              G^GT[ACGT]ACC
              990  GGTTACC             
Cfr10I          7  ACCGGT               [GA]^CCGG[CT]
              533  ACCGGC              
EcoO65I       291  GGTCACC              G^GT[ACGT]ACC
              990  GGTTACC             
EcoT22I        58  ATGCAT               ATGCA^T
              833  ATGCAT              
HgaI          762  GACGC                GACGC(5/10)
              900  GCGTC               
Hin1I         899  GGCGTC               G[GA]^CG[CT]C
              907  GGCGCC              
MaeI          317  CTAG                 C^TAG
              667  CTAG                
MseI           28  TTAA                 T^TAA
              107  TTAA                
NsiI           58  ATGCAT               ATGCA^T
              833  ATGCAT              
Tru9I          28  TTAA                 T^TAA
              107  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   52 enzymes) #
#########################################

AfaI        AluI        AlwI        AvaII       BbvI        
BsaWI       BsiYI       BslI        BsmFI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeIII      MboI        MboII       MflI        MnlI        
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       TfiI        TseI        Tsp45I      Tsp509I     
TspRI       XhoII       
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