Restriction map for pF1KA2011
 (Linear) (Six-base) MAPSORT of: KIAA2011.seq  Check: 5072  from: 1  to: 3072



KIAA2011.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:56  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     999    1060    2697    3072 
   Size:        999      61    1637     375

  Fragments arranged by size: 

                1637     999     375      61
 
AccI GT'mk_AC

Cuts at:      0    2270    3072 
   Size:       2270     802
 
Acc65I G'GTAC_C

Cuts at:      0    1405    3072 
   Size:       1405    1667
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1133    1695    1740    1858    1943    2752    2825    2908 
   Size:       1133     562      45     118      85     809      73      83

Cuts at:   2908    3072 
   Size:        164

  Fragments arranged by size: 

                1133     809     562     164     118      85      83      73
                  45
 
AfeI AGC'GCT

Cuts at:      0    2679    2870    3072 
   Size:       2679     191     202

  Fragments arranged by size: 

                2679     202     191
 
AflIII A'CryG_T

Cuts at:      0     183    3072 
   Size:        183    2889
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1638    3072 
   Size:       1638    1434
 
AlwNI CAG_nnn'CTG

Cuts at:      0     979    1680    2311    2635    3072 
   Size:        979     701     631     324     437

  Fragments arranged by size: 

                 979     701     631     437     324
 
ApaI G_GGCC'C

Cuts at:      0     288     595     970    1122    2286    3072 
   Size:        288     307     375     152    1164     786

  Fragments arranged by size: 

                1164     786     375     307     288     152
 
ApaLI G'TGCA_C

Cuts at:      0    2299    3072 
   Size:       2299     773
 
ApoI r'AATT_y

Cuts at:      0     721    3072 
   Size:        721    2351
 
AvaI C'yCGr_G

Cuts at:      0     164     280     674    2251    2330    2553    3011    3072 
   Size:        164     116     394    1577      79     223     458      61

  Fragments arranged by size: 

                1577     458     394     223     164     116      79      61
            
 
AvrII C'CTAG_G

Cuts at:      0    2074    3072 
   Size:       2074     998
 
BamHI G'GATC_C

Cuts at:      0    2363    3072 
   Size:       2363     709
 
BanI G'GyrC_C

Cuts at:      0      10      90     249     870    1249    1405    1733    1945 
   Size:         10      80     159     621     379     156     328     212

Cuts at:   1945    3072 
   Size:       1127

  Fragments arranged by size: 

                1127     621     379     328     212     159     156      80
                  10
 
BanII G_rGCy'C

Cuts at:      0     103     288     295     595     805     817     970    1122 
   Size:        103     185       7     300     210      12     153     152

Cuts at:   1122    1180    1312    1496    2286    2438    3018    3072 
   Size:         58     132     184     790     152     580      54

  Fragments arranged by size: 

                 790     580     300     210     185     184     153     152
                 152     132     103      58      54      12       7
 
BbeI G_GCGC'C

Cuts at:      0    1949    3072 
   Size:       1949    1123
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1788    2329    2499    2529    3072 
   Size:       1788     541     170      30     543

  Fragments arranged by size: 

                1788     543     541     170      30
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     651    3072 
   Size:        651    2421
 
BglI GCCn_nnn'nGGC

Cuts at:      0     937     974    1477    1726    3072 
   Size:        937      37     503     249    1346

  Fragments arranged by size: 

                1346     937     503     249      37
 
BlpI GC'TnA_GC

Cuts at:      0     761     917    3017    3072 
   Size:        761     156    2100      55

  Fragments arranged by size: 

                2100     761     156      55
 
Bme1580I G_kGCm'C

Cuts at:      0      95     252     288     595     875     970    1122    1160 
   Size:         95     157      36     307     280      95     152      38

Cuts at:   1160    2286    2303    3072 
   Size:       1126      17     769

  Fragments arranged by size: 

                1126     769     307     280     157     152      95      95
                  38      36      17
 
BmgBI CAC'GTC

Cuts at:      0     184    2505    3072 
   Size:        184    2321     567

  Fragments arranged by size: 

                2321     567     184
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     187     275    3072 
   Size:        187      88    2797

  Fragments arranged by size: 

                2797     187      88
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     951    2174    3072 
   Size:        951    1223     898

  Fragments arranged by size: 

                1223     951     898
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     341    1249    1297    1684    2065    2211    2581    2854 
   Size:        341     908      48     387     381     146     370     273

Cuts at:   2854    2958    2985    3072 
   Size:        104      27      87

  Fragments arranged by size: 

                 908     387     381     370     341     273     146     104
                  87      48      27
 
Bpu10I CC'TnA_GC

Cuts at:      0     599     753    1564    3072 
   Size:        599     154     811    1508

  Fragments arranged by size: 

                1508     811     599     154
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     387     789    1129    1768    2071    2353    2422    3072 
   Size:        387     402     340     639     303     282      69     650

  Fragments arranged by size: 

                 650     639     402     387     340     303     282      69
            
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1855    3072 
   Size:       1855    1217
 
BsaHI Gr'CG_yC

Cuts at:      0    1946    3072 
   Size:       1946    1126
 
BsaWI w'CCGG_w

Cuts at:      0     437    1395    3072 
   Size:        437     958    1677

  Fragments arranged by size: 

                1677     958     437
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     389     500     558     675     940    1462    1465    1468 
   Size:        389     111      58     117     265     522       3       3

Cuts at:   1468    2155    2158    2413    2716    2917    3072 
   Size:        687       3     255     303     201     155

  Fragments arranged by size: 

                 687     522     389     303     265     255     201     155
                 117     111      58       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     105     377     414     585     748     955    1027    1530 
   Size:        105     272      37     171     163     207      72     503

Cuts at:   1530    1771    1898    2628    2674    2771    2775    3072 
   Size:        241     127     730      46      97       4     297

  Fragments arranged by size: 

                 730     503     297     272     241     207     171     163
                 127     105      97      72      46      37       4
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1331    3072 
   Size:       1331    1741
 
BsiHKAI G_wGCw'C

Cuts at:      0     295    2303    2438    2762    2947    3072 
   Size:        295    2008     135     324     185     125

  Fragments arranged by size: 

                2008     324     295     185     135     125
 
BsmI GAATG_Cn'

Cuts at:      0    2448    3072 
   Size:       2448     624
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2145    2422    3072 
   Size:       2145     277     650

  Fragments arranged by size: 

                2145     650     277
 
Bsp1286I G_dGCh'C

Cuts at:      0      95     103     252     288     295     595     805     817 
   Size:         95       8     149      36       7     300     210      12

Cuts at:    817     875     970    1122    1160    1180    1312    1496    2286 
   Size:         58      95     152      38      20     132     184     790

Cuts at:   2286    2303    2438    2762    2947    3018    3072 
   Size:         17     135     324     185      71      54

  Fragments arranged by size: 

                 790     324     300     210     185     184     152     149
                 135     132      95      95      71      58      54      38
                  36      20      17      12       8       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     142     360     636     999    1060    2029    2396    2522 
   Size:        142     218     276     363      61     969     367     126

Cuts at:   2522    2697    3072 
   Size:        175     375

  Fragments arranged by size: 

                 969     375     367     363     276     218     175     142
                 126      61
 
BsrBI CCG'CTC

Cuts at:      0     921    1611    3072 
   Size:        921     690    1461

  Fragments arranged by size: 

                1461     921     690
 
BsrDI GCAATG_nn'

Cuts at:      0    1050    3072 
   Size:       1050    2022
 
BsrGI T'GTAC_A

Cuts at:      0     199     519    2096    3072 
   Size:        199     320    1577     976

  Fragments arranged by size: 

                1577     976     320     199
 
BssSI C'ACGA_G

Cuts at:      0    2474    2719    3072 
   Size:       2474     245     353

  Fragments arranged by size: 

                2474     353     245
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2021    3072 
   Size:       2021    1051
 
BstBI TT'CG_AA

Cuts at:      0    1217    3072 
   Size:       1217    1855
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      71     585    3072 
   Size:         71     514    2487

  Fragments arranged by size: 

                2487     514      71
 
BstYI r'GATC_y

Cuts at:      0     381    1986    2363    3072 
   Size:        381    1605     377     709

  Fragments arranged by size: 

                1605     709     381     377
 
Bsu36I CC'TnA_GG

Cuts at:      0     877    3072 
   Size:        877    2195
 
BtgI C'CryG_G

Cuts at:      0     997    1992    2358    2738    3072 
   Size:        997     995     366     380     334

  Fragments arranged by size: 

                 997     995     380     366     334
 
BtsI GCAGTG_nn'

Cuts at:      0     977    1044    2131    2220    2226    2904    2921    3060 
   Size:        977      67    1087      89       6     678      17     139

Cuts at:   3060    3072 
   Size:         12

  Fragments arranged by size: 

                1087     977     678     139      89      67      17      12
                   6
 
ClaI AT'CG_AT

Cuts at:      0     902    3072 
   Size:        902    2170
 
DraIII CAC_nnn'GTG

Cuts at:      0     229    3072 
   Size:        229    2843
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2533    3072 
   Size:       2533     539
 
EaeI y'GGCC_r

Cuts at:      0     608     938    1123    1682    1995    2171    2468    3040 
   Size:        608     330     185     559     313     176     297     572

Cuts at:   3040    3072 
   Size:         32

  Fragments arranged by size: 

                 608     572     559     330     313     297     185     176
                  32
 
EarI CTCTTCn'nnn_

Cuts at:      0     261     622    2344    2548    2801    3072 
   Size:        261     361    1722     204     253     271

  Fragments arranged by size: 

                1722     361     271     261     253     204
 
EcoICRI GAG'CTC

Cuts at:      0     293    2436    3072 
   Size:        293    2143     636

  Fragments arranged by size: 

                2143     636     293
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     341    1133    1249    1297    1684    1695    1740    1858 
   Size:        341     792     116      48     387      11      45     118

Cuts at:   1858    1943    2065    2211    2581    2752    2825    2854    2908 
   Size:         85     122     146     370     171      73      29      54

Cuts at:   2908    2958    2985    3072 
   Size:         50      27      87

  Fragments arranged by size: 

                 792     387     370     341     171     146     122     118
                 116      87      85      73      54      50      48      45
                  29      27      11
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     356     649    2392    2545    2695    3041    3072 
   Size:        356     293    1743     153     150     346      31

  Fragments arranged by size: 

                1743     356     346     293     153     150      31
 
EcoO109I rG'GnC_Cy

Cuts at:      0     160     284     591     592     625     777     966    1118 
   Size:        160     124     307       1      33     152     189     152

Cuts at:   1118    2049    2282    2283    2418    2498    2550    3072 
   Size:        931     233       1     135      80      52     522

  Fragments arranged by size: 

                 931     522     307     233     189     160     152     152
                 135     124      80      52      33       1       1
 
EcoRI G'AATT_C

Cuts at:      0     721    3072 
   Size:        721    2351
 
FspI TGC'GCA

Cuts at:      0    1673    2518    3072 
   Size:       1673     845     554

  Fragments arranged by size: 

                1673     845     554
 
HaeII r_GCGC'y

Cuts at:      0     606    1680    1949    2681    2872    3072 
   Size:        606    1074     269     732     191     200

  Fragments arranged by size: 

                1074     732     606     269     200     191
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     366     398     649     681    1573    1605    1624    1656 
   Size:        366      32     251      32     892      32      19      32

Cuts at:   1656    3072 
   Size:       1416

  Fragments arranged by size: 

                1416     892     366     251      32      32      32      32
                  19
 
KasI G'GCGC_C

Cuts at:      0    1945    3072 
   Size:       1945    1127
 
KpnI G_GTAC'C

Cuts at:      0    1409    3072 
   Size:       1409    1663
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     352     458     528    1604    3072 
   Size:        352     106      70    1076    1468

  Fragments arranged by size: 

                1468    1076     352     106      70
 
MscI TGG'CCA

Cuts at:      0     610    1684    2470    3072 
   Size:        610    1074     786     602

  Fragments arranged by size: 

                1074     786     610     602
 
MslI CAynn'nnrTG

Cuts at:      0      57    1804    3072 
   Size:         57    1747    1268

  Fragments arranged by size: 

                1747    1268      57
 
MspA1I CmG'CkG

Cuts at:      0      71    1572    2207    2524    2632    2775    2814    3072 
   Size:         71    1501     635     317     108     143      39     258

  Fragments arranged by size: 

                1501     635     317     258     143     108      71      39
            
 
NarI GG'CG_CC

Cuts at:      0    1946    3072 
   Size:       1946    1126
 
NcoI C'CATG_G

Cuts at:      0    2738    3072 
   Size:       2738     334
 
NspI r_CATG'y

Cuts at:      0     117    3072 
   Size:        117    2955
 
PasI CC'CwG_GG

Cuts at:      0       6     245    1483    1620    3072 
   Size:          6     239    1238     137    1452

  Fragments arranged by size: 

                1452    1238     239     137       6
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     343     421    1015    2466    3072 
   Size:        343      78     594    1451     606

  Fragments arranged by size: 

                1451     606     594     343      78
 
PfoI T'CCnGG_A

Cuts at:      0    1226    2237    2575    3072 
   Size:       1226    1011     338     497

  Fragments arranged by size: 

                1226    1011     497     338
 
PpuMI rG'GwC_Cy

Cuts at:      0     160     625    3072 
   Size:        160     465    2447

  Fragments arranged by size: 

                2447     465     160
 
PspOMI G'GGCC_C

Cuts at:      0     284     591     966    1118    2282    3072 
   Size:        284     307     375     152    1164     790

  Fragments arranged by size: 

                1164     790     375     307     284     152
 
PspXI vC'TCGA_Gb

Cuts at:      0     164    3072 
   Size:        164    2908
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1771    1803    3072 
   Size:       1771      32    1269

  Fragments arranged by size: 

                1771    1269      32
 
PstI C_TGCA'G

Cuts at:      0    2126    2320    2393    2990    3072 
   Size:       2126     194      73     597      82

  Fragments arranged by size: 

                2126     597     194      82      73
 
PvuII CAG'CTG

Cuts at:      0    1572    2524    2632    2775    3072 
   Size:       1572     952     108     143     297

  Fragments arranged by size: 

                1572     952     297     143     108
 
SacI G_AGCT'C

Cuts at:      0     295    2438    3072 
   Size:        295    2143     634

  Fragments arranged by size: 

                2143     634     295
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2344    2801    3072 
   Size:       2344     457     271

  Fragments arranged by size: 

                2344     457     271
 
ScaI AGT'ACT

Cuts at:      0     847    3072 
   Size:        847    2225
 
SexAI A'CCwGG_T

Cuts at:      0     661    3072 
   Size:        661    2411
 
SfcI C'TryA_G

Cuts at:      0    2122    2316    2389    2487    2853    2986    3072 
   Size:       2122     194      73      98     366     133      86

  Fragments arranged by size: 

                2122     366     194     133      98      86      73
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     937    1726    3072 
   Size:        937     789    1346

  Fragments arranged by size: 

                1346     937     789
 
SfoI GGC'GCC

Cuts at:      0    1947    3072 
   Size:       1947    1125
 
SmaI CCC'GGG

Cuts at:      0     282     676    3013    3072 
   Size:        282     394    2337      59

  Fragments arranged by size: 

                2337     394     282      59
 
SmlI C'TyrA_G

Cuts at:      0     164     366     804    1144    1783    2086    2368    2437 
   Size:        164     202     438     340     639     303     282      69

Cuts at:   2437    3072 
   Size:        635

  Fragments arranged by size: 

                 639     635     438     340     303     282     202     164
                  69
 
StuI AGG'CCT

Cuts at:      0    1108    1282    2092    2827    3072 
   Size:       1108     174     810     735     245

  Fragments arranged by size: 

                1108     810     735     245     174
 
StyI C'CwwG_G

Cuts at:      0     420     737    1283    1429    1524    1545    2074    2186 
   Size:        420     317     546     146      95      21     529     112

Cuts at:   2186    2738    3072 
   Size:        552     334

  Fragments arranged by size: 

                 552     546     529     420     334     317     146     112
                  95      21
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     276     376    1846    3072 
   Size:        276     100    1470    1226

  Fragments arranged by size: 

                1470    1226     276     100
 
TatI w'GTAC_w

Cuts at:      0     199     519     845    2096    2402    3072 
   Size:        199     320     326    1251     306     670

  Fragments arranged by size: 

                1251     670     326     320     306     199
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1473    2235    3072 
   Size:       1473     762     837

  Fragments arranged by size: 

                1473     837     762
 
XhoI C'TCGA_G

Cuts at:      0     164    3072 
   Size:        164    2908
 
XmaI C'CCGG_G

Cuts at:      0     280     674    3011    3072 
   Size:        280     394    2337      61

  Fragments arranged by size: 

                2337     394     280      61
 
XmnI GAAnn'nnTTC

Cuts at:      0    2353    3072 
   Size:       2353     719
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AfeI   AflIII     AhdI    AlwNI
     ApaI    ApaLI     ApoI     AvaI    AvrII    BamHI     BanI    BanII
     BbeI     BbsI    BciVI     BglI     BlpI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI    BsrBI
    BsrDI    BsrGI    BssSI   BstAPI    BstBI    BstXI    BstYI   Bsu36I
     BtgI     BtsI     ClaI   DraIII     DrdI     EaeI     EarI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FspI    HaeII    Hin4I     KasI
     KpnI     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NspI
     PasI    PflMI     PfoI    PpuMI   PspOMI    PspXI     PsrI     PstI
    PvuII     SacI     SapI     ScaI    SexAI     SfcI     SfiI     SfoI
     SmaI     SmlI     StuI     StyI    TaqII     TatI     XcmI     XhoI
     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AclI    AflII     AgeI     AleI     AlfI     AloI     AscI
     AseI    AsiSI     BaeI    BbvCI     BcgI     BclI    BfrBI    BglII
     BmtI    BsaAI    BsaBI    BsaXI    BsiEI    BsiWI    BspEI    BspHI
    BsrFI   BssHII   BstEII  BstZ17I    BtgZI    CspCI     DraI     EagI
     EciI    EcoRV     FalI     FseI    FspAI   HincII  HindIII     HpaI
     MfeI     MluI     NaeI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI
     PvuI    RsrII    SacII     SalI    SanDI     SbfI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TstI  Tth111I
     XbaI     ZraI
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