Restriction map for
pF1KA1779
(Linear) (Six-base) MAPSORT of: KIAA1779.seq Check: 3511 from: 1 to: 3630
KIAA1779.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2391 3630
Size: 2391 1239
Acc65I G'GTAC_C
Cuts at: 0 3387 3630
Size: 3387 243
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 350 962 1684 2128 2693 2797 3630
Size: 176 174 612 722 444 565 104 833
Fragments arranged by size:
833 722 612 565 444 176 174 104
AfeI AGC'GCT
Cuts at: 0 2991 3045 3630
Size: 2991 54 585
Fragments arranged by size:
2991 585 54
AflIII A'CryG_T
Cuts at: 0 2034 2864 3630
Size: 2034 830 766
Fragments arranged by size:
2034 830 766
AgeI A'CCGG_T
Cuts at: 0 2932 3630
Size: 2932 698
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1406 2346 3630
Size: 1406 940 1284
Fragments arranged by size:
1406 1284 940
AlwNI CAG_nnn'CTG
Cuts at: 0 906 1869 2053 3612 3630
Size: 906 963 184 1559 18
Fragments arranged by size:
1559 963 906 184 18
ApaLI G'TGCA_C
Cuts at: 0 433 2936 3630
Size: 433 2503 694
Fragments arranged by size:
2503 694 433
ApoI r'AATT_y
Cuts at: 0 1220 2026 2101 3630
Size: 1220 806 75 1529
Fragments arranged by size:
1529 1220 806 75
AvaI C'yCGr_G
Cuts at: 0 25 85 2364 3630
Size: 25 60 2279 1266
Fragments arranged by size:
2279 1266 60 25
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 383 416 3630
Size: 383 33 3214
Fragments arranged by size:
3214 383 33
BamHI G'GATC_C
Cuts at: 0 638 800 3630
Size: 638 162 2830
Fragments arranged by size:
2830 638 162
BanI G'GyrC_C
Cuts at: 0 1340 2394 2762 3387 3630
Size: 1340 1054 368 625 243
Fragments arranged by size:
1340 1054 625 368 243
BanII G_rGCy'C
Cuts at: 0 85 558 1141 1586 1860 1985 2045 2371
Size: 85 473 583 445 274 125 60 326
Cuts at: 2371 3335 3630
Size: 964 295
Fragments arranged by size:
964 583 473 445 326 295 274 125
85 60
BbsI GAAGACnn'nnnn_
Cuts at: 0 482 659 1745 3428 3626 3630
Size: 482 177 1086 1683 198 4
Fragments arranged by size:
1683 1086 482 198 177 4
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1111 1145 3630
Size: 1111 34 2485
Fragments arranged by size:
2485 1111 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2550 3630
Size: 2550 1080
BlpI GC'TnA_GC
Cuts at: 0 557 1885 3630
Size: 557 1328 1745
Fragments arranged by size:
1745 1328 557
Bme1580I G_kGCm'C
Cuts at: 0 437 1343 2399 2765 2872 2940 3630
Size: 437 906 1056 366 107 68 690
Fragments arranged by size:
1056 906 690 437 366 107 68
BmrI ACTGGGnnnn_n'
Cuts at: 0 799 3456 3630
Size: 799 2657 174
Fragments arranged by size:
2657 799 174
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2708 3630
Size: 2708 922
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1110 1308 2828 3360 3630
Size: 1110 198 1520 532 270
Fragments arranged by size:
1520 1110 532 270 198
Bpu10I CC'TnA_GC
Cuts at: 0 41 925 3362 3494 3630
Size: 41 884 2437 132 136
Fragments arranged by size:
2437 884 136 132 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 503 3515 3630
Size: 364 139 3012 115
Fragments arranged by size:
3012 364 139 115
BsaAI yAC'GTr
Cuts at: 0 1251 2037 2867 3630
Size: 1251 786 830 763
Fragments arranged by size:
1251 830 786 763
BsaHI Gr'CG_yC
Cuts at: 0 487 3630
Size: 487 3143
BsaWI w'CCGG_w
Cuts at: 0 615 686 2484 2932 3630
Size: 615 71 1798 448 698
Fragments arranged by size:
1798 698 615 448 71
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2725 2755 3630
Size: 2725 30 875
Fragments arranged by size:
2725 875 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 361 785 1311 1366 1719 1865 1868 2340
Size: 361 424 526 55 353 146 3 472
Cuts at: 2340 2746 3499 3630
Size: 406 753 131
Fragments arranged by size:
753 526 472 424 406 361 353 146
131 55 3
BseYI C'CCAG_C
Cuts at: 0 725 885 1797 2398 2421 2436 2532 3121
Size: 725 160 912 601 23 15 96 589
Cuts at: 3121 3175 3630
Size: 54 455
Fragments arranged by size:
912 725 601 589 455 160 96 54
23 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 581 919 2465 3630
Size: 581 338 1546 1165
Fragments arranged by size:
1546 1165 581 338
BsiEI CG_ry'CG
Cuts at: 0 2215 2219 3630
Size: 2215 4 1411
Fragments arranged by size:
2215 1411 4
BsiHKAI G_wGCw'C
Cuts at: 0 315 437 558 615 1613 1860 2940 3630
Size: 315 122 121 57 998 247 1080 690
Fragments arranged by size:
1080 998 690 315 247 122 121 57
BsmI GAATG_Cn'
Cuts at: 0 3368 3630
Size: 3368 262
BsmBI CGTCTCn'nnnn_
Cuts at: 0 24 201 1348 3630
Size: 24 177 1147 2282
Fragments arranged by size:
2282 1147 177 24
Bsp1286I G_dGCh'C
Cuts at: 0 85 315 437 558 615 1141 1343 1586
Size: 85 230 122 121 57 526 202 243
Cuts at: 1586 1613 1860 1985 2045 2371 2399 2765 2872
Size: 27 247 125 60 326 28 366 107
Cuts at: 2872 2940 3335 3630
Size: 68 395 295
Fragments arranged by size:
526 395 366 326 295 247 243 230
202 125 122 121 107 85 68 60
57 28 27
BspEI T'CCGG_A
Cuts at: 0 686 3630
Size: 686 2944
BspHI T'CATG_A
Cuts at: 0 3374 3630
Size: 3374 256
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 515 941 1374 2391 3630
Size: 515 426 433 1017 1239
Fragments arranged by size:
1239 1017 515 433 426
BsrBI CCG'CTC
Cuts at: 0 75 3630
Size: 75 3555
BsrDI GCAATG_nn'
Cuts at: 0 2687 3630
Size: 2687 943
BsrFI r'CCGG_y
Cuts at: 0 2218 2287 2932 3630
Size: 2218 69 645 698
Fragments arranged by size:
2218 698 645 69
BsrGI T'GTAC_A
Cuts at: 0 335 711 1574 1708 3630
Size: 335 376 863 134 1922
Fragments arranged by size:
1922 863 376 335 134
BstBI TT'CG_AA
Cuts at: 0 1131 3630
Size: 1131 2499
BstEII G'GTnAC_C
Cuts at: 0 889 2951 3167 3630
Size: 889 2062 216 463
Fragments arranged by size:
2062 889 463 216
BstYI r'GATC_y
Cuts at: 0 638 800 1865 2114 3630
Size: 638 162 1065 249 1516
Fragments arranged by size:
1516 1065 638 249 162
Bsu36I CC'TnA_GG
Cuts at: 0 317 899 3630
Size: 317 582 2731
Fragments arranged by size:
2731 582 317
BtgI C'CryG_G
Cuts at: 0 6 642 1874 2342 2390 2945 3053 3107
Size: 6 636 1232 468 48 555 108 54
Cuts at: 3107 3161 3630
Size: 54 469
Fragments arranged by size:
1232 636 555 469 468 108 54 54
48 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1299 1894 3630
Size: 1299 595 1736
Fragments arranged by size:
1736 1299 595
BtsI GCAGTG_nn'
Cuts at: 0 545 881 2916 3630
Size: 545 336 2035 714
Fragments arranged by size:
2035 714 545 336
ClaI AT'CG_AT
Cuts at: 0 919 3630
Size: 919 2711
DraI TTT'AAA
Cuts at: 0 2031 3630
Size: 2031 1599
DraIII CAC_nnn'GTG
Cuts at: 0 1347 3630
Size: 1347 2283
DrdI GACnn_nn'nnGTC
Cuts at: 0 2590 3630
Size: 2590 1040
EaeI y'GGCC_r
Cuts at: 0 353 2216 3590 3630
Size: 353 1863 1374 40
Fragments arranged by size:
1863 1374 353 40
EagI C'GGCC_G
Cuts at: 0 2216 3630
Size: 2216 1414
EarI CTCTTCn'nnn_
Cuts at: 0 1660 1978 2075 2451 2655 2813 3323 3630
Size: 1660 318 97 376 204 158 510 307
Fragments arranged by size:
1660 510 376 318 307 204 158 97
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 127 1378 3630
Size: 127 1251 2252
Fragments arranged by size:
2252 1251 127
EcoICRI GAG'CTC
Cuts at: 0 556 1858 3630
Size: 556 1302 1772
Fragments arranged by size:
1772 1302 556
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 350 962 1110 1308 1684 2128 2693
Size: 176 174 612 148 198 376 444 565
Cuts at: 2693 2797 2828 3360 3630
Size: 104 31 532 270
Fragments arranged by size:
612 565 532 444 376 270 198 176
174 148 104 31
EcoNI CCTnn'n_nnAGG
Cuts at: 0 898 3630
Size: 898 2732
EcoO109I rG'GnC_Cy
Cuts at: 0 51 903 2406 3630
Size: 51 852 1503 1224
Fragments arranged by size:
1503 1224 852 51
EcoRV GAT'ATC
Cuts at: 0 2660 3630
Size: 2660 970
HaeII r_GCGC'y
Cuts at: 0 2993 3047 3630
Size: 2993 54 583
Fragments arranged by size:
2993 583 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 285 317 450 482 1034 1066 2099 2131
Size: 285 32 133 32 552 32 1033 32
Cuts at: 2131 2956 2988 3513 3545 3630
Size: 825 32 525 32 85
Fragments arranged by size:
1033 825 552 525 285 133 85 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 504 3630
Size: 504 3126
KpnI G_GTAC'C
Cuts at: 0 3391 3630
Size: 3391 239
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2713 3630
Size: 2713 917
MscI TGG'CCA
Cuts at: 0 355 3630
Size: 355 3275
MslI CAynn'nnrTG
Cuts at: 0 1879 3373 3630
Size: 1879 1494 257
Fragments arranged by size:
1879 1494 257
MspA1I CmG'CkG
Cuts at: 0 8 254 595 1265 1289 1337 2050 2344
Size: 8 246 341 670 24 48 713 294
Cuts at: 2344 3580 3626 3630
Size: 1236 46 4
Fragments arranged by size:
1236 713 670 341 294 246 48 46
24 8 4
NaeI GCC'GGC
Cuts at: 0 2220 3630
Size: 2220 1410
NcoI C'CATG_G
Cuts at: 0 2390 3107 3630
Size: 2390 717 523
Fragments arranged by size:
2390 717 523
NgoMIV G'CCGG_C
Cuts at: 0 2218 3630
Size: 2218 1412
NspI r_CATG'y
Cuts at: 0 336 2001 3630
Size: 336 1665 1629
Fragments arranged by size:
1665 1629 336
PasI CC'CwG_GG
Cuts at: 0 54 841 2960 3630
Size: 54 787 2119 670
Fragments arranged by size:
2119 787 670 54
PflMI CCAn_nnn'nTGG
Cuts at: 0 1828 3630
Size: 1828 1802
PmlI CAC'GTG
Cuts at: 0 1251 2037 2867 3630
Size: 1251 786 830 763
Fragments arranged by size:
1251 830 786 763
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2275 2307 3630
Size: 2275 32 1323
Fragments arranged by size:
2275 1323 32
PpuMI rG'GwC_Cy
Cuts at: 0 903 3630
Size: 903 2727
PspXI vC'TCGA_Gb
Cuts at: 0 2364 3630
Size: 2364 1266
PstI C_TGCA'G
Cuts at: 0 101 637 2227 3630
Size: 101 536 1590 1403
Fragments arranged by size:
1590 1403 536 101
PvuII CAG'CTG
Cuts at: 0 595 3580 3626 3630
Size: 595 2985 46 4
Fragments arranged by size:
2985 595 46 4
SacI G_AGCT'C
Cuts at: 0 558 1860 3630
Size: 558 1302 1770
Fragments arranged by size:
1770 1302 558
SacII CC_GC'GG
Cuts at: 0 9 2345 3630
Size: 9 2336 1285
Fragments arranged by size:
2336 1285 9
SapI GCTCTTCn'nnn_
Cuts at: 0 1660 2075 3323 3630
Size: 1660 415 1248 307
Fragments arranged by size:
1660 1248 415 307
SexAI A'CCwGG_T
Cuts at: 0 3383 3630
Size: 3383 247
SfcI C'TryA_G
Cuts at: 0 97 633 2130 2223 3630
Size: 97 536 1497 93 1407
Fragments arranged by size:
1497 1407 536 97 93
SmaI CCC'GGG
Cuts at: 0 27 3630
Size: 27 3603
SmlI C'TyrA_G
Cuts at: 0 343 518 2364 3530 3630
Size: 343 175 1846 1166 100
Fragments arranged by size:
1846 1166 343 175 100
SpeI A'CTAG_T
Cuts at: 0 3241 3630
Size: 3241 389
SspI AAT'ATT
Cuts at: 0 2173 3630
Size: 2173 1457
StuI AGG'CCT
Cuts at: 0 2492 3630
Size: 2492 1138
StyI C'CwwG_G
Cuts at: 0 2390 3107 3630
Size: 2390 717 523
Fragments arranged by size:
2390 717 523
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2086 3630
Size: 2086 1544
TatI w'GTAC_w
Cuts at: 0 335 711 1030 1574 1708 3411 3630
Size: 335 376 319 544 134 1703 219
Fragments arranged by size:
1703 544 376 335 319 219 134
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 416 448 1603 1635 2725 2757 3630
Size: 416 32 1155 32 1090 32 873
Fragments arranged by size:
1155 1090 873 416 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1648 2211 3630
Size: 1648 563 1419
Fragments arranged by size:
1648 1419 563
XbaI T'CTAG_A
Cuts at: 0 228 3630
Size: 228 3402
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3469 3630
Size: 3469 161
XhoI C'TCGA_G
Cuts at: 0 2364 3630
Size: 2364 1266
XmaI C'CCGG_G
Cuts at: 0 25 3630
Size: 25 3605
XmnI GAAnn'nnTTC
Cuts at: 0 773 3198 3311 3630
Size: 773 2425 113 319
Fragments arranged by size:
2425 773 319 113
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AgeI AlfI AlwNI
ApaLI ApoI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV
HaeII Hin4I HincII KpnI MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NspI PasI PflMI PmlI PpiI
PpuMI PspXI PstI PvuII SacI SacII SapI SexAI
SfcI SmaI SmlI SpeI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AflII AhdI AleI AloI ApaI
AscI AseI AsiSI AvrII BbeI BbvCI BclI BfrBI
BglI BglII BmgBI BmtI BsaI BsaBI BsiWI BssHII
BssSI BstAPI BstXI BstZ17I CspCI EcoRI FalI FseI
FspI FspAI HindIII HpaI KasI MfeI MluI NarI
NdeI NheI NotI NruI NsiI PacI PciI PfoI
PmeI PshAI PsiI PspOMI PsrI PvuI RsrII SalI
SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI
SphI SrfI SwaI ZraI