Restriction map for pF1KA1761
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# NO CUT ENZYMES (   99 enzymes) #
##################################

Acc65I      AclI        AflII       AgeI        AhdI        
Aor51HI     AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AviII       AvrII       BalI        
BbeI        BbrPI       BbvCI       BclI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsiEI       BsiWI       BspDI       
BspHI       BspLU11I    BssHII      BssSI       Bst1107I    
BstBI       BstZ17I     CelII       ClaI        DraI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoRV       EcoT22I     FbaI        FseI        
FspI        HgaI        HincII      HindII      HindIII     
HpaI        KasI        KpnI        MamI        MfeI        
MluI        MluNI       MscI        MunI        NarI        
NcoI        NdeI        NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        PvuII       RcaI        SalI        SbfI        
ScaI        SfiI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        SwaI        XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AatII        1639  GACGTC               GACGT^C
AccI         1780  GTCTAC               GT^[AC][GT]AC
AccIII        314  TCCGGA               T^CCGGA
AcyI         1639  GACGTC               G[GA]^CG[CT]C
AflIII       1424  ACACGT               A^C[GA][CT]GT
Alw44I        885  GTGCAC               G^TGCAC
ApaLI         885  GTGCAC               G^TGCAC
AspI         2202  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanI          102  GGCACC               G^G[CT][GA]CC
BcgI          238  GCAGAGCTCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         351  GGATAC               GTATCC(6/5)
BsaHI        1639  GACGTC               G[GA]^CG[CT]C
BsaI          682  GAGACC               GGTCTC(1/5)
BseAI         314  TCCGGA               T^CCGGA
BsmBI        1180  GAGACG               CGTCTC(1/5)
Bsp1407I      767  TGTACA               T^GTACA
BspEI         314  TCCGGA               T^CCGGA
BspMI        1960  GCAGGT               ACCTGC(4/8)
BsrDI        2261  GCAATG               GCAATG(2/0)
BsrGI         767  TGTACA               T^GTACA
BstAPI       1923  GCATGAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        807  GGTGACC              G^GT[ACGT]ACC
BstPI         807  GGTGACC              G^GT[ACGT]ACC
CpoI           18  CGGTCCG              CG^G[AT]CCG
DrdI          643  GACCTCAGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I       807  GGTGACC              G^GT[ACGT]ACC
EcoRI         277  GAATTC               G^AATTC
HaeII        2342  GGCGCT               [GA]GCGC^[CT]
Hin1I        1639  GACGTC               G[GA]^CG[CT]C
MroI          314  TCCGGA               T^CCGGA
PflFI        2202  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         650  GGGTCCT              [GA]G^G[AT]CC[CT]
PshAI        1162  GACAACAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII          18  CGGTCCG              CG^G[AT]CCG
SacI          241  GAGCTC               GAGCT^C
SapI         2497  GAAGAGC              GCTCTTC(1/4)
SexAI        1808  ACCTGGT              A^CC[AT]GGT
SmaI           97  CCCGGG               CCC^GGG
SspBI         767  TGTACA               T^GTACA
Tth111I      2202  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          377  CCATGAGGCTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI          97  CCCGGG               C^CCGGG
XmaI           97  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AlwNI        1010  CAGCATCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1804  CAGCACCTG           
BamHI         317  GGATCC               G^GATCC
             1110  GGATCC              
BsaWI         314  TCCGGA               [AT]^CCGG[AT]
              365  ACCGGA              
BsgI          474  GTGCAG               GTGCAG(16/14)
              493  GTGCAG              
BsmFI        2285  GGGAC                GGGAC(10/14)
             1510  GTCCC               
BsmI         1826  GCATTC               GAATGC(1/-1)
             2046  GCATTC              
BsrBI        1470  CCGCTC               CCGCTC(-3/-3)
             1105  GAGCGG              
BsrFI           6  GCCGGC               [GA]^CCGG[CT]
               75  GCCGGC              
BstXI         620  CCATCTCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2162  CCAAATACTTGG        
Cfr10I          6  GCCGGC               [GA]^CCGG[CT]
               75  GCCGGC              
DraII         650  GGGTCCT              [GA]G^G[ACGT]CC[CT]
             2099  GGGCCCT             
DraIII       1807  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1912  CACTGCGTG           
EcoO109I      650  GGGTCCT              [GA]G^G[ACGT]CC[CT]
             2099  GGGCCCT             
KspI          106  CCGCGG               CCGC^GG
              116  CCGCGG              
MseI         1724  TTAA                 T^TAA
             1766  TTAA                
NaeI            6  GCCGGC               GCC^GGC
               75  GCCGGC              
NgoMIV          6  GCCGGC               G^CCGGC
               75  GCCGGC              
PaeR7I        434  CTCGAG               C^TCGAG
             1133  CTCGAG              
SacII         106  CCGCGG               CCGC^GG
              116  CCGCGG              
TfiI          288  GAATC                G^A[AT]TC
              981  GATTC               
Tru9I        1724  TTAA                 T^TAA
             1766  TTAA                
XhoI          434  CTCGAG               C^TCGAG
             1133  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   94 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AocI        ApaI        ApoI        AspHI       
AvaI        AvaII       BanII       BbsI        BbvI        
BcnI        BfaI        BmyI        BpmI        BpuAI       
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsoBI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstUI       BstYI       Bsu36I      
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DsaI        EarI        Eco81I      EcoNI       EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PflMI       PleI        PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XhoII       
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