Restriction map for pF1KA1761
 (Linear) (Six-base) MAPSORT of: KIAA1761.seq  Check: 3070  from: 1  to: 2511



KIAA1761.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:55  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1951    2511 
   Size:       1951     560
 
AatII G_ACGT'C

Cuts at:      0    1643    2511 
   Size:       1643     868
 
AccI GT'mk_AC

Cuts at:      0    1781    2511 
   Size:       1781     730
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     726    1004    1594    2161    2511 
   Size:        726     278     590     567     350

  Fragments arranged by size: 

                 726     590     567     350     278
 
AflIII A'CryG_T

Cuts at:      0    1424    2511 
   Size:       1424    1087
 
AleI CACnn'nnGTG

Cuts at:      0    1366    1666    2511 
   Size:       1366     300     845

  Fragments arranged by size: 

                1366     845     300
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1529    2511 
   Size:       1529     982
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1015    1809    2511 
   Size:       1015     794     702

  Fragments arranged by size: 

                1015     794     702
 
ApaI G_GGCC'C

Cuts at:      0      28    2103    2358    2511 
   Size:         28    2075     255     153

  Fragments arranged by size: 

                2075     255     153      28
 
ApaLI G'TGCA_C

Cuts at:      0     885    2511 
   Size:        885    1626
 
ApoI r'AATT_y

Cuts at:      0     277     814    1327    2511 
   Size:        277     537     513    1184

  Fragments arranged by size: 

                1184     537     513     277
 
AvaI C'yCGr_G

Cuts at:      0      97     434    1133    2511 
   Size:         97     337     699    1378

  Fragments arranged by size: 

                1378     699     337      97
 
BamHI G'GATC_C

Cuts at:      0     317    1110    2511 
   Size:        317     793    1401

  Fragments arranged by size: 

                1401     793     317
 
BanI G'GyrC_C

Cuts at:      0     102    2511 
   Size:        102    2409
 
BanII G_rGCy'C

Cuts at:      0      28     245     760    1265    1275    2103    2358    2511 
   Size:         28     217     515     505      10     828     255     153

  Fragments arranged by size: 

                 828     515     505     255     217     153      28      10
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     880    2206    2466    2511 
   Size:        880    1326     260      45

  Fragments arranged by size: 

                1326     880     260      45
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     227     261    2511 
   Size:        227      34    2250

  Fragments arranged by size: 

                2250     227      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     345    2511 
   Size:        345    2166
 
Bme1580I G_kGCm'C

Cuts at:      0      28     105     541     889    2103    2310    2358    2511 
   Size:         28      77     436     348    1214     207      48     153

  Fragments arranged by size: 

                1214     436     348     207     153      77      48      28
            
 
BmgBI CAC'GTC

Cuts at:      0    1427    2511 
   Size:       1427    1084
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2259    2511 
   Size:       2259     252
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     460     495     774    2493    2511 
   Size:        460      35     279    1719      18

  Fragments arranged by size: 

                1719     460     279      35      18
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     223     550    1192    2004    2511 
   Size:        223     327     642     812     507

  Fragments arranged by size: 

                 812     642     507     327     223
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     409     440    1330    1741    2134    2511 
   Size:        409      31     890     411     393     377

  Fragments arranged by size: 

                 890     411     409     393     377      31
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     676    2511 
   Size:        676    1835
 
BsaHI Gr'CG_yC

Cuts at:      0    1640    2511 
   Size:       1640     871
 
BsaWI w'CCGG_w

Cuts at:      0     314     365    2511 
   Size:        314      51    2146

  Fragments arranged by size: 

                2146     314      51
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     196     226    1456    1486    2511 
   Size:        196      30    1230      30    1025

  Fragments arranged by size: 

                1230    1025     196      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     319     514     919    1162    1471    1666    2314    2511 
   Size:        319     195     405     243     309     195     648     197

  Fragments arranged by size: 

                 648     405     319     309     243     197     195     195
            
 
BseYI C'CCAG_C

Cuts at:      0      53     753    1543    1686    1802    2511 
   Size:         53     700     790     143     116     709

  Fragments arranged by size: 

                 790     709     700     143     116      53
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     495     514    2511 
   Size:        495      19    1997

  Fragments arranged by size: 

                1997     495      19
 
BsiHKAI G_wGCw'C

Cuts at:      0     245     889    1064    2511 
   Size:        245     644     175    1447

  Fragments arranged by size: 

                1447     644     245     175
 
BsmI GAATG_Cn'

Cuts at:      0    1826    2046    2511 
   Size:       1826     220     465

  Fragments arranged by size: 

                1826     465     220
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1174    2511 
   Size:       1174    1337
 
Bsp1286I G_dGCh'C

Cuts at:      0      28     105     245     541     760     889    1064    1265 
   Size:         28      77     140     296     219     129     175     201

Cuts at:   1265    1275    2103    2310    2358    2511 
   Size:         10     828     207      48     153

  Fragments arranged by size: 

                 828     296     219     207     201     175     153     140
                 129      77      48      28      10
 
BspEI T'CCGG_A

Cuts at:      0     314    2511 
   Size:        314    2197
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1951    2511 
   Size:       1951     560
 
BsrBI CCG'CTC

Cuts at:      0    1107    1472    2511 
   Size:       1107     365    1039

  Fragments arranged by size: 

                1107    1039     365
 
BsrDI GCAATG_nn'

Cuts at:      0    2268    2511 
   Size:       2268     243
 
BsrFI r'CCGG_y

Cuts at:      0       6      75    2511 
   Size:          6      69    2436

  Fragments arranged by size: 

                2436      69       6
 
BsrGI T'GTAC_A

Cuts at:      0     767    2511 
   Size:        767    1744
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1929    2511 
   Size:       1929     582
 
BstEII G'GTnAC_C

Cuts at:      0     807    2511 
   Size:        807    1704
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     627    2169    2511 
   Size:        627    1542     342

  Fragments arranged by size: 

                1542     627     342
 
BstYI r'GATC_y

Cuts at:      0     317    1110    1577    2018    2511 
   Size:        317     793     467     441     493

  Fragments arranged by size: 

                 793     493     467     441     317
 
Bsu36I CC'TnA_GG

Cuts at:      0     646     962    1649    2429    2511 
   Size:        646     316     687     780      82

  Fragments arranged by size: 

                 780     687     646     316      82
 
BtgI C'CryG_G

Cuts at:      0     106     116    1267    2511 
   Size:        106      10    1151    1244

  Fragments arranged by size: 

                1244    1151     106      10
 
BtsI GCAGTG_nn'

Cuts at:      0     404     606     828    1356    1874    1911    2185    2511 
   Size:        404     202     222     528     518      37     274     326

  Fragments arranged by size: 

                 528     518     404     326     274     222     202      37
            
 
DraIII CAC_nnn'GTG

Cuts at:      0    1812    1917    2511 
   Size:       1812     105     594

  Fragments arranged by size: 

                1812     594     105
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     649    2511 
   Size:        649    1862
 
EarI CTCTTCn'nnn_

Cuts at:      0     412    1133    1139    2492    2511 
   Size:        412     721       6    1353      19

  Fragments arranged by size: 

                1353     721     412      19       6
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      59    1960    2511 
   Size:         59    1901     551

  Fragments arranged by size: 

                1901     551      59
 
EcoICRI GAG'CTC

Cuts at:      0     243    2511 
   Size:        243    2268
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     223     550     726    1004    1192    1594    2004    2161 
   Size:        223     327     176     278     188     402     410     157

Cuts at:   2161    2511 
   Size:        350

  Fragments arranged by size: 

                 410     402     350     327     278     223     188     176
                 157
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     529    1481    2428    2511 
   Size:        529     952     947      83

  Fragments arranged by size: 

                 952     947     529      83
 
EcoO109I rG'GnC_Cy

Cuts at:      0     651    2100    2511 
   Size:        651    1449     411

  Fragments arranged by size: 

                1449     651     411
 
EcoRI G'AATT_C

Cuts at:      0     277    2511 
   Size:        277    2234
 
HaeII r_GCGC'y

Cuts at:      0    2346    2511 
   Size:       2346     165
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     481     513     675     707    2511 
   Size:        481      32     162      32    1804

  Fragments arranged by size: 

                1804     481     162      32      32
 
MslI CAynn'nnrTG

Cuts at:      0     976    1300    1366    1666    2511 
   Size:        976     324      66     300     845

  Fragments arranged by size: 

                 976     845     324     300      66
 
MspA1I CmG'CkG

Cuts at:      0     108     118     765    2511 
   Size:        108      10     647    1746

  Fragments arranged by size: 

                1746     647     108      10
 
NaeI GCC'GGC

Cuts at:      0       8      77    2511 
   Size:          8      69    2434

  Fragments arranged by size: 

                2434      69       8
 
NgoMIV G'CCGG_C

Cuts at:      0       6      75    2511 
   Size:          6      69    2436

  Fragments arranged by size: 

                2436      69       6
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1809    2418    2492    2511 
   Size:       1809     609      74      19

  Fragments arranged by size: 

                1809     609      74      19
 
PfoI T'CCnGG_A

Cuts at:      0     386     499     527    1898    2511 
   Size:        386     113      28    1371     613

  Fragments arranged by size: 

                1371     613     386     113      28
 
PpuMI rG'GwC_Cy

Cuts at:      0     651    2511 
   Size:        651    1860
 
PshAI GACnn'nnGTC

Cuts at:      0    1166    2511 
   Size:       1166    1345
 
PspOMI G'GGCC_C

Cuts at:      0      24    2099    2354    2511 
   Size:         24    2075     255     157

  Fragments arranged by size: 

                2075     255     157      24
 
PspXI vC'TCGA_Gb

Cuts at:      0     434    2511 
   Size:        434    2077
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1765    1797    2511 
   Size:       1765      32     714

  Fragments arranged by size: 

                1765     714      32
 
PstI C_TGCA'G

Cuts at:      0     240     598     914    1518    1593    2399    2510    2511 
   Size:        240     358     316     604      75     806     111       1

  Fragments arranged by size: 

                 806     604     358     316     240     111      75       1
            
 
RsrII CG'GwC_CG

Cuts at:      0      19    2511 
   Size:         19    2492
 
SacI G_AGCT'C

Cuts at:      0     245    2511 
   Size:        245    2266
 
SacII CC_GC'GG

Cuts at:      0     109     119    2511 
   Size:        109      10    2392

  Fragments arranged by size: 

                2392     109      10
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2492    2511 
   Size:       2492      19
 
SexAI A'CCwGG_T

Cuts at:      0    1808    2511 
   Size:       1808     703
 
SfcI C'TryA_G

Cuts at:      0     236     594     910    1222    1514    1589    2395    2506 
   Size:        236     358     316     312     292      75     806     111

Cuts at:   2506    2511 
   Size:          5

  Fragments arranged by size: 

                 806     358     316     312     292     236     111      75
                   5
 
SmaI CCC'GGG

Cuts at:      0      99    2511 
   Size:         99    2412
 
SmlI C'TyrA_G

Cuts at:      0     424     434     455    1133    1309    1756    2149    2511 
   Size:        424      10      21     678     176     447     393     362

  Fragments arranged by size: 

                 678     447     424     393     362     176      21      10
            
 
StyI C'CwwG_G

Cuts at:      0    1277    1530    2281    2511 
   Size:       1277     253     751     230

  Fragments arranged by size: 

                1277     751     253     230
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1244    1611    2511 
   Size:       1244     367     900

  Fragments arranged by size: 

                1244     900     367
 
TatI w'GTAC_w

Cuts at:      0     767    1421    2511 
   Size:        767     654    1090

  Fragments arranged by size: 

                1090     767     654
 
Tth111I GACn'n_nGTC

Cuts at:      0    2205    2511 
   Size:       2205     306
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     384    2511 
   Size:        384    2127
 
XhoI C'TCGA_G

Cuts at:      0     434    1133    2511 
   Size:        434     699    1378

  Fragments arranged by size: 

                1378     699     434
 
XmaI C'CCGG_G

Cuts at:      0      97    2511 
   Size:         97    2414
 
ZraI GAC'GTC

Cuts at:      0    1641    2511 
   Size:       1641     870
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI   AflIII     AleI     AlfI    AlwNI
     ApaI    ApaLI     ApoI     AvaI    BamHI     BanI    BanII     BbsI
     BcgI    BciVI Bme1580I    BmgBI     BmrI     BplI     BpmI    BpuEI
     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI
   DraIII     DrdI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    HaeII    Hin4I     MslI   MspA1I     NaeI   NgoMIV    PflMI
     PfoI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI    RsrII
     SacI    SacII     SapI    SexAI     SfcI     SmaI     SmlI     StyI
    TaqII     TatI  Tth111I     XcmI     XhoI     XmaI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AloI     AscI
     AseI    AsiSI    AvrII     BaeI     BbeI    BbvCI     BclI    BfrBI
     BglI    BglII     BlpI     BmtI   Bpu10I    BsaAI    BsaBI    BsiEI
    BsiWI    BspHI   BssHII    BssSI    BstBI  BstZ17I    BtgZI     ClaI
    CspCI     DraI     EaeI     EagI    EcoRV     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     NarI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PmeI     PmlI     PpiI
     PsiI     PvuI    PvuII     SalI    SanDI     SbfI     ScaI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TstI     XbaI     XmnI
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