Restriction map for pF1KA1668
##################################
# NO CUT ENZYMES (   84 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        ApoI        
AscI        AseI        AsnI        AspEI       AvrII       
BcgI        BfrI        BglII       BlnI        BsaBI       
BseAI       BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspLU11I    BsrDI       BsrGI       
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
ClaI        CpoI        DraI        Eam1105I    EcoO65I     
EcoRI       EcoRV       EcoT22I     FseI        HincII      
HindII      HindIII     HpaI        MamI        MfeI        
MluI        MroI        MunI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflMI       PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SalI        
SapI        ScaI        SfiI        SfuI        SgrAI       
SnaBI       SphI        SspBI       SspI        SwaI        
Tsp509I     Van91I      XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

Acc65I       1636  GGTACC               G^GTACC
AcyI         1795  GGCGCC               G[GA]^CG[CT]C
Alw44I        511  GTGCAC               G^TGCAC
ApaLI         511  GTGCAC               G^TGCAC
Asp700I      2466  GAAAGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1636  GGTACC               G^GTACC
AviII         490  TGCGCA               TGC^GCA
BbeI         1795  GGCGCC               GGCGC^C
BbrPI         486  CACGTG               CAC^GTG
BciVI        2064  GGATAC               GTATCC(6/5)
BclI         2456  TGATCA               T^GATCA
BglI         1965  GCCAGTGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         1524  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1524  GCTCAGC              GC^T[ACGT]AGC
BsaAI         486  CACGTG               [CT]AC^GT[GA]
BsaHI        1795  GGCGCC               G[GA]^CG[CT]C
BsaI          255  GGTCTC               GGTCTC(1/5)
BspHI         287  TCATGA               T^CATGA
BssSI        2188  CACGAG               CACGAG(-5/-1)
CelII        1524  GCTCAGC              GC^T[ACGT]AGC
DraIII        514  CACTTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          291  GACCTATGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          829  CGGCCG               C^GGCCG
EclXI         829  CGGCCG               C^GGCCG
Eco52I        829  CGGCCG               C^GGCCG
FbaI         2456  TGATCA               T^GATCA
FspI          490  TGCGCA               TGC^GCA
Hin1I        1795  GGCGCC               G[GA]^CG[CT]C
KasI         1795  GGCGCC               G^GCGCC
KpnI         1636  GGTACC               GGTAC^C
MaeIII       2365  GTGAC                ^GT[ACGT]AC
MseI         1904  TTAA                 T^TAA
NaeI         2078  GCCGGC               GCC^GGC
NarI         1795  GGCGCC               GG^CGCC
NgoMIV       2078  GCCGGC               G^CCGGC
PmaCI         486  CACGTG               CAC^GTG
PmlI          486  CACGTG               CAC^GTG
RcaI          287  TCATGA               T^CATGA
SbfI          784  CCTGCAGG             CCTGCA^GG
SexAI         986  ACCTGGT              A^CC[AT]GGT
SfoI         1795  GGCGCC               GGC^GCC
SmlI         1724  CTCAAG               C^T[CT][GA]AG
SpeI         1695  ACTAGT               A^CTAGT
Sse8387I      784  CCTGCAGG             CCTGCA^GG
Tru9I        1904  TTAA                 T^TAA
Tsp45I       2365  GTGAC                ^GT[GC]AC
XmnI         2466  GAAAGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

Aor51HI       524  AGCGCT               AGC^GCT
             1521  AGCGCT              
BamHI         224  GGATCC               G^GATCC
             1957  GGATCC              
BbvCI         279  CCTCAGC              CCTCAGC(-5/-2)
              793  GCTGAGG             
BsiEI         676  CGGTCG               CG[GA][CT]^CG
              829  CGGCCG              
BsrBI         230  CCGCTC               CCGCTC(-3/-3)
              963  CCGCTC              
BssHII         17  GCGCGC               G^CGCGC
             1440  GCGCGC              
EarI         1306  GAAGAG               CTCTTC(1/4)
             2428  GAAGAG              
Eco47III      524  AGCGCT               AGC^GCT
             1521  AGCGCT              
EcoNI        2136  CCTGAATGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2403  CCTGGATGAGG         
Ksp632I      1306  GAAGAG               CTCTTC(1/4)
             2428  GAAGAG              
KspI           10  CCGCGG               CCGC^GG
               57  CCGCGG              
MaeII         487  ACGT                 A^CGT
             2553  ACGT                
NcoI         1108  CCATGG               C^CATGG
             2052  CCATGG              
NspI          507  GCATGT               [GA]CATG^[CT]
             2159  ACATGC              
PpuMI        1100  AGGACCC              [GA]G^G[AT]CC[CT]
             1767  AGGTCCC             
SacII          10  CCGCGG               CCGC^GG
               57  CCGCGG              
StuI          688  AGGCCT               AGG^CCT
              920  AGGCCT              
TaqI          179  TCGA                 T^CGA
             2273  TCGA                
TfiI          154  GATTC                G^A[AT]TC
             1896  GAATC               
TthHB8I       179  TCGA                 T^CGA
             2273  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (  107 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       AocI        ApaI        AspHI       AspI        
AvaI        AvaII       BalI        BanI        BanII       
BbsI        BbvI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaJI       BsaWI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrFI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstUI       BstXI       BstYI       Bsu36I      Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        Eco81I      EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeI        MboI        MboII       MflI        MluNI       
MnlI        MscI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PflFI       PleI        PstI        
PvuII       RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmaI        StyI        
TseI        TspRI       Tth111I     XcmI        XhoII       
XmaCI       XmaI        
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