Restriction map for pF1KA1668
 (Linear) (Six-base) MAPSORT of: KIAA1668.seq  Check: 4707  from: 1  to: 2589



KIAA1668.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:55  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1191    1558    1929    1986    2589 
   Size:       1191     367     371      57     603

  Fragments arranged by size: 

                1191     603     371     367      57
 
Acc65I G'GTAC_C

Cuts at:      0    1636    2589 
   Size:       1636     953
 
AfeI AGC'GCT

Cuts at:      0     526    1523    2589 
   Size:        526     997    1066

  Fragments arranged by size: 

                1066     997     526
 
AleI CACnn'nnGTG

Cuts at:      0     400     634    1120    2589 
   Size:        400     234     486    1469

  Fragments arranged by size: 

                1469     486     400     234
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1028    1060    2589 
   Size:       1028      32    1529

  Fragments arranged by size: 

                1529    1028      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     793     876    1618    2250    2589 
   Size:        793      83     742     632     339

  Fragments arranged by size: 

                 793     742     632     339      83
 
ApaI G_GGCC'C

Cuts at:      0     667     698    2337    2589 
   Size:        667      31    1639     252

  Fragments arranged by size: 

                1639     667     252      31
 
ApaLI G'TGCA_C

Cuts at:      0     511    2589 
   Size:        511    2078
 
AvaI C'yCGr_G

Cuts at:      0     666     851    1173    2336    2589 
   Size:        666     185     322    1163     253

  Fragments arranged by size: 

                1163     666     322     253     185
 
BamHI G'GATC_C

Cuts at:      0     224    1957    2589 
   Size:        224    1733     632

  Fragments arranged by size: 

                1733     632     224
 
BanI G'GyrC_C

Cuts at:      0      32     128     463     628    1216    1636    1795    1839 
   Size:         32      96     335     165     588     420     159      44

Cuts at:   1839    2288    2589 
   Size:        449     301

  Fragments arranged by size: 

                 588     449     420     335     301     165     159      96
                  44      32
 
BanII G_rGCy'C

Cuts at:      0     437     667     698    1243    1609    1837    2225    2285 
   Size:        437     230      31     545     366     228     388      60

Cuts at:   2285    2337    2589 
   Size:         52     252

  Fragments arranged by size: 

                 545     437     388     366     252     230     228      60
                  52      31
 
BbeI G_GCGC'C

Cuts at:      0    1799    2589 
   Size:       1799     790
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     168    2226    2441    2589 
   Size:        168    2058     215     148

  Fragments arranged by size: 

                2058     215     168     148
 
BbvCI CC'TCA_GC

Cuts at:      0     280     794    2589 
   Size:        280     514    1795

  Fragments arranged by size: 

                1795     514     280
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2058    2589 
   Size:       2058     531
 
BclI T'GATC_A

Cuts at:      0    2456    2589 
   Size:       2456     133
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1971    2589 
   Size:       1971     618
 
BlpI GC'TnA_GC

Cuts at:      0    1525    2589 
   Size:       1525    1064
 
Bme1580I G_kGCm'C

Cuts at:      0     466     515     631     667     698    1601    2337    2589 
   Size:        466      49     116      36      31     903     736     252

  Fragments arranged by size: 

                 903     736     466     252     116      49      36      31
            
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     297    2589 
   Size:        297    2292
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     453     456    1518    2589 
   Size:        453       3    1062    1071

  Fragments arranged by size: 

                1071    1062     453       3
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     383     567     759     951    1276    1298    1603    2113 
   Size:        383     184     192     192     325      22     305     510

Cuts at:   2113    2134    2272    2589 
   Size:         21     138     317

  Fragments arranged by size: 

                 510     383     325     317     305     192     192     184
                 138      22      21
 
Bpu10I CC'TnA_GC

Cuts at:      0      79     280     422     794    1764    2589 
   Size:         79     201     142     372     970     825

  Fragments arranged by size: 

                 970     825     372     201     142      79
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1709    2589 
   Size:       1709     880
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     261    2589 
   Size:        261    2328
 
BsaAI yAC'GTr

Cuts at:      0     488    2589 
   Size:        488    2101
 
BsaHI Gr'CG_yC

Cuts at:      0    1796    2589 
   Size:       1796     793
 
BsaWI w'CCGG_w

Cuts at:      0     566    1907    2285    2589 
   Size:        566    1341     378     304

  Fragments arranged by size: 

                1341     566     378     304
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     241     271    1451    1481    1525    1555    2589 
   Size:        241      30    1180      30      44      30    1034

  Fragments arranged by size: 

                1180    1034     241      44      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     429     445     637    1237    1297    1300    1303    1306 
   Size:        429      16     192     600      60       3       3       3

Cuts at:   1306    1599    1662    1674    2056    2131    2284    2338    2589 
   Size:        293      63      12     382      75     153      54     251

  Fragments arranged by size: 

                 600     429     382     293     251     192     153      75
                  63      60      54      16      12       3       3       3
            
 
BseYI C'CCAG_C

Cuts at:      0       4     219     478     660     769     939    1232    1324 
   Size:          4     215     259     182     109     170     293      92

Cuts at:   1324    1447    1772    1932    2589 
   Size:        123     325     160     657

  Fragments arranged by size: 

                 657     325     293     259     215     182     170     160
                 123     109      92       4
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     541     662    1015    1144    1304    1363    1592 
   Size:        106     435     121     353     129     160      59     229

Cuts at:   1592    2589 
   Size:        997

  Fragments arranged by size: 

                 997     435     353     229     160     129     121     106
                  59
 
BsiEI CG_ry'CG

Cuts at:      0     679     832    2589 
   Size:        679     153    1757

  Fragments arranged by size: 

                1757     679     153
 
BsiHKAI G_wGCw'C

Cuts at:      0     437     515     583     783     934    1609    2099    2225 
   Size:        437      78      68     200     151     675     490     126

Cuts at:   2225    2285    2589 
   Size:         60     304

  Fragments arranged by size: 

                 675     490     437     304     200     151     126      78
                  68      60
 
Bsp1286I G_dGCh'C

Cuts at:      0     437     466     515     583     631     667     698     783 
   Size:        437      29      49      68      48      36      31      85

Cuts at:    783     934    1243    1601    1609    1837    2099    2225    2285 
   Size:        151     309     358       8     228     262     126      60

Cuts at:   2285    2337    2589 
   Size:         52     252

  Fragments arranged by size: 

                 437     358     309     262     252     228     151     126
                  85      68      60      52      49      48      36      31
                  29       8
 
BspHI T'CATG_A

Cuts at:      0     287    2589 
   Size:        287    2302
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     149    1191    1558    1863    1929    1986    2589 
   Size:        149    1042     367     305      66      57     603

  Fragments arranged by size: 

                1042     603     367     305     149      66      57
 
BsrBI CCG'CTC

Cuts at:      0     232     965    2589 
   Size:        232     733    1624

  Fragments arranged by size: 

                1624     733     232
 
BsrFI r'CCGG_y

Cuts at:      0     125     131     466    2078    2414    2589 
   Size:        125       6     335    1612     336     175

  Fragments arranged by size: 

                1612     336     335     175     125       6
 
BssHII G'CGCG_C

Cuts at:      0      17    1440    2589 
   Size:         17    1423    1149

  Fragments arranged by size: 

                1423    1149      17
 
BssSI C'ACGA_G

Cuts at:      0    2188    2589 
   Size:       2188     401
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     488     510     519     742    1038    2589 
   Size:        488      22       9     223     296    1551

  Fragments arranged by size: 

                1551     488     296     223      22       9
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     252     402    1633    2589 
   Size:        252     150    1231     956

  Fragments arranged by size: 

                1231     956     252     150
 
BstYI r'GATC_y

Cuts at:      0      68     224     836    1957    2589 
   Size:         68     156     612    1121     632

  Fragments arranged by size: 

                1121     632     612     156      68
 
Bsu36I CC'TnA_GG

Cuts at:      0     441     620     821    2039    2492    2589 
   Size:        441     179     201    1218     453      97

  Fragments arranged by size: 

                1218     453     441     201     179      97
 
BtgI C'CryG_G

Cuts at:      0      10      57    1108    2052    2100    2589 
   Size:         10      47    1051     944      48     489

  Fragments arranged by size: 

                1051     944     489      48      47      10
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     538    1499    2589 
   Size:        538     961    1090

  Fragments arranged by size: 

                1090     961     538
 
BtsI GCAGTG_nn'

Cuts at:      0     558    1797    2589 
   Size:        558    1239     792

  Fragments arranged by size: 

                1239     792     558
 
DraIII CAC_nnn'GTG

Cuts at:      0     519    2589 
   Size:        519    2070
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     297    2589 
   Size:        297    2292
 
EaeI y'GGCC_r

Cuts at:      0     330     468     829     878    1247    1331    1505    2589 
   Size:        330     138     361      49     369      84     174    1084

  Fragments arranged by size: 

                1084     369     361     330     174     138      84      49
            
 
EagI C'GGCC_G

Cuts at:      0     829    2589 
   Size:        829    1760
 
EarI CTCTTCn'nnn_

Cuts at:      0    1301    2423    2589 
   Size:       1301    1122     166

  Fragments arranged by size: 

                1301    1122     166
 
EcoICRI GAG'CTC

Cuts at:      0     435    1607    2223    2283    2589 
   Size:        435    1172     616      60     306

  Fragments arranged by size: 

                1172     616     435     306      60
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     383     567     759     951    1276    1298    1603    2113 
   Size:        383     184     192     192     325      22     305     510

Cuts at:   2113    2134    2272    2589 
   Size:         21     138     317

  Fragments arranged by size: 

                 510     383     325     317     305     192     192     184
                 138      22      21
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2140    2407    2589 
   Size:       2140     267     182

  Fragments arranged by size: 

                2140     267     182
 
EcoO109I rG'GnC_Cy

Cuts at:      0     360     695    1101    1768    2589 
   Size:        360     335     406     667     821

  Fragments arranged by size: 

                 821     667     406     360     335
 
FspI TGC'GCA

Cuts at:      0     492    2589 
   Size:        492    2097
 
HaeII r_GCGC'y

Cuts at:      0     528    1431    1525    1556    1799    2079    2262    2589 
   Size:        528     903      94      31     243     280     183     327

  Fragments arranged by size: 

                 903     528     327     280     243     183      94      31
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     216     241     248     273    1525    1557    2589 
   Size:        216      25       7      25    1252      32    1032

  Fragments arranged by size: 

                1252    1032     216      32      25      25       7
 
KasI G'GCGC_C

Cuts at:      0    1795    2589 
   Size:       1795     794
 
KpnI G_GTAC'C

Cuts at:      0    1640    2589 
   Size:       1640     949
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1803    2426    2589 
   Size:       1803     623     163

  Fragments arranged by size: 

                1803     623     163
 
MscI TGG'CCA

Cuts at:      0     332     880    1333    2589 
   Size:        332     548     453    1256

  Fragments arranged by size: 

                1256     548     453     332
 
MslI CAynn'nnrTG

Cuts at:      0     400     634    1120    2164    2527    2589 
   Size:        400     234     486    1044     363      62

  Fragments arranged by size: 

                1044     486     400     363     234      62
 
MspA1I CmG'CkG

Cuts at:      0      12      59    1318    1594    1869    2589 
   Size:         12      47    1259     276     275     720

  Fragments arranged by size: 

                1259     720     276     275      47      12
 
NaeI GCC'GGC

Cuts at:      0    2080    2589 
   Size:       2080     509
 
NarI GG'CG_CC

Cuts at:      0    1796    2589 
   Size:       1796     793
 
NcoI C'CATG_G

Cuts at:      0    1108    2052    2589 
   Size:       1108     944     537

  Fragments arranged by size: 

                1108     944     537
 
NgoMIV G'CCGG_C

Cuts at:      0    2078    2589 
   Size:       2078     511
 
NspI r_CATG'y

Cuts at:      0     511    2163    2589 
   Size:        511    1652     426

  Fragments arranged by size: 

                1652     511     426
 
PasI CC'CwG_GG

Cuts at:      0     598    1347    2589 
   Size:        598     749    1242

  Fragments arranged by size: 

                1242     749     598
 
PfoI T'CCnGG_A

Cuts at:      0      92     236     756    2589 
   Size:         92     144     520    1833

  Fragments arranged by size: 

                1833     520     144      92
 
PmlI CAC'GTG

Cuts at:      0     488    2589 
   Size:        488    2101
 
PpuMI rG'GwC_Cy

Cuts at:      0    1101    1768    2589 
   Size:       1101     667     821

  Fragments arranged by size: 

                1101     821     667
 
PspOMI G'GGCC_C

Cuts at:      0     663     694    2333    2589 
   Size:        663      31    1639     256

  Fragments arranged by size: 

                1639     663     256      31
 
PstI C_TGCA'G

Cuts at:      0     325     789     977    1569    1792    1874    1926    2589 
   Size:        325     464     188     592     223      82      52     663

  Fragments arranged by size: 

                 663     592     464     325     223     188      82      52
            
 
PvuII CAG'CTG

Cuts at:      0    1318    1594    1869    2589 
   Size:       1318     276     275     720

  Fragments arranged by size: 

                1318     720     276     275
 
SacI G_AGCT'C

Cuts at:      0     437    1609    2225    2285    2589 
   Size:        437    1172     616      60     304

  Fragments arranged by size: 

                1172     616     437     304      60
 
SacII CC_GC'GG

Cuts at:      0      13      60    2589 
   Size:         13      47    2529

  Fragments arranged by size: 

                2529      47      13
 
SbfI CC_TGCA'GG

Cuts at:      0     789    2589 
   Size:        789    1800
 
SexAI A'CCwGG_T

Cuts at:      0     986    2589 
   Size:        986    1603
 
SfcI C'TryA_G

Cuts at:      0     321     386     785     868     973    1565    1788    1870 
   Size:        321      65     399      83     105     592     223      82

Cuts at:   1870    1922    2589 
   Size:         52     667

  Fragments arranged by size: 

                 667     592     399     321     223     105      83      82
                  65      52
 
SfoI GGC'GCC

Cuts at:      0    1797    2589 
   Size:       1797     792
 
SmaI CCC'GGG

Cuts at:      0     668     853    1175    2338    2589 
   Size:        668     185     322    1163     251

  Fragments arranged by size: 

                1163     668     322     251     185
 
SmlI C'TyrA_G

Cuts at:      0    1724    2589 
   Size:       1724     865
 
SpeI A'CTAG_T

Cuts at:      0    1695    2589 
   Size:       1695     894
 
StuI AGG'CCT

Cuts at:      0     690     922    2589 
   Size:        690     232    1667

  Fragments arranged by size: 

                1667     690     232
 
StyI C'CwwG_G

Cuts at:      0     164     746     809    1108    1952    2052    2589 
   Size:        164     582      63     299     844     100     537

  Fragments arranged by size: 

                 844     582     537     299     164     100      63
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     366     408    1338    1383    1461    2589 
   Size:        366      42     930      45      78    1128

  Fragments arranged by size: 

                1128     930     366      78      45      42
 
TatI w'GTAC_w

Cuts at:      0    2030    2589 
   Size:       2030     559
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1345    1377    1451    1483    2589 
   Size:       1345      32      74      32    1106

  Fragments arranged by size: 

                1345    1106      74      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     172     253     675    2589 
   Size:        172      81     422    1914

  Fragments arranged by size: 

                1914     422     172      81
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1344    1674    2312    2589 
   Size:       1344     330     638     277

  Fragments arranged by size: 

                1344     638     330     277
 
XmaI C'CCGG_G

Cuts at:      0     666     851    1173    2336    2589 
   Size:        666     185     322    1163     253

  Fragments arranged by size: 

                1163     666     322     253     185
 
XmnI GAAnn'nnTTC

Cuts at:      0    2470    2589 
   Size:       2470     119
 

 Enzymes that do cut:

     AarI   Acc65I     AfeI     AleI     AloI    AlwNI     ApaI    ApaLI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI    BciVI
     BclI     BglI     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrBI    BsrFI
   BssHII    BssSI   BstAPI    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI   DraIII     DrdI     EaeI     EagI     EarI  EcoICRI  Eco57MI
    EcoNI EcoO109I     FspI    HaeII    Hin4I     KasI     KpnI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PasI     PfoI     PmlI    PpuMI   PspOMI     PstI    PvuII     SacI
    SacII     SbfI    SexAI     SfcI     SfoI     SmaI     SmlI     SpeI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AcuI    AflII   AflIII     AgeI     AhdI
     AlfI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BcgI
    BfrBI    BglII    BmgBI     BmtI    BsaBI    BsiWI     BsmI    BsmBI
    BspEI    BsrDI    BsrGI    BstBI   BstEII  BstZ17I     ClaI    CspCI
     DraI     EciI    EcoRI    EcoRV     FalI     FseI    FspAI   HincII
  HindIII     HpaI     MfeI     MluI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PciI    PflMI     PmeI     PpiI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII     SalI    SanDI     SapI     ScaI
     SfiI     SgfI    SgrAI    SnaBI     SphI     SrfI     SspI     SwaI
     XbaI     XhoI     ZraI
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