Restriction map for pF1KA1534
 (Linear) (Six-base) MAPSORT of: KIAA1534.seq  Check: 3766  from: 1  to: 2433



KIAA1534.orf

 With 182 enzymes: SgfI * 

                        February 20, 2009 16:05  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     559    1209    2433 
   Size:        559     650    1224

  Fragments arranged by size: 

                1224     650     559
 
AccI GT'mk_AC

Cuts at:      0      68    2433 
   Size:         68    2365
 
Acc65I G'GTAC_C

Cuts at:      0    1091    2433 
   Size:       1091    1342
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     418     447     940    1969    2433 
   Size:        418      29     493    1029     464

  Fragments arranged by size: 

                1029     493     464     418      29
 
AfeI AGC'GCT

Cuts at:      0     357    2433 
   Size:        357    2076
 
AflIII A'CryG_T

Cuts at:      0    1613    2433 
   Size:       1613     820
 
AleI CACnn'nnGTG

Cuts at:      0     208     823    2433 
   Size:        208     615    1610

  Fragments arranged by size: 

                1610     615     208
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     658     690     988    1020    2433 
   Size:        658      32     298      32    1413

  Fragments arranged by size: 

                1413     658     298      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     441    1689    1937    2247    2289    2433 
   Size:        441    1248     248     310      42     144

  Fragments arranged by size: 

                1248     441     310     248     144      42
 
ApaI G_GGCC'C

Cuts at:      0     185     406     801    1839    2045    2433 
   Size:        185     221     395    1038     206     388

  Fragments arranged by size: 

                1038     395     388     221     206     185
 
ApoI r'AATT_y

Cuts at:      0    1501    2433 
   Size:       1501     932
 
AvaI C'yCGr_G

Cuts at:      0    1551    1662    2433 
   Size:       1551     111     771

  Fragments arranged by size: 

                1551     771     111
 
BamHI G'GATC_C

Cuts at:      0    1979    2131    2433 
   Size:       1979     152     302

  Fragments arranged by size: 

                1979     302     152
 
BanI G'GyrC_C

Cuts at:      0      23     210     547    1091    1704    2297    2340    2433 
   Size:         23     187     337     544     613     593      43      93

  Fragments arranged by size: 

                 613     593     544     337     187      93      43      23
            
 
BanII G_rGCy'C

Cuts at:      0     119     152     185     406     427     801    1839    2045 
   Size:        119      33      33     221      21     374    1038     206

Cuts at:   2045    2159    2206    2320    2368    2433 
   Size:        114      47     114      48      65

  Fragments arranged by size: 

                1038     374     221     206     119     114     114      65
                  48      47      33      33      21
 
BbeI G_GCGC'C

Cuts at:      0      27    2433 
   Size:         27    2406
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     665     766    2433 
   Size:        665     101    1667

  Fragments arranged by size: 

                1667     665     101
 
BbvCI CC'TCA_GC

Cuts at:      0     100     627    1690    2433 
   Size:        100     527    1063     743

  Fragments arranged by size: 

                1063     743     527     100
 
BfrBI ATG'CAT

Cuts at:      0     691    2433 
   Size:        691    1742
 
BglI GCCn_nnn'nGGC

Cuts at:      0     401    1595    2433 
   Size:        401    1194     838

  Fragments arranged by size: 

                1194     838     401
 
BglII A'GATC_T

Cuts at:      0    1547    2433 
   Size:       1547     886
 
BlpI GC'TnA_GC

Cuts at:      0     352    2314    2433 
   Size:        352    1962     119

  Fragments arranged by size: 

                1962     352     119
 
Bme1580I G_kGCm'C

Cuts at:      0     185     406     550     801    1839    2045    2433 
   Size:        185     221     144     251    1038     206     388

  Fragments arranged by size: 

                1038     388     251     221     206     185     144
 
BmgBI CAC'GTC

Cuts at:      0    1254    1565    1614    1755    2433 
   Size:       1254     311      49     141     678

  Fragments arranged by size: 

                1254     678     311     141      49
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     553    1611    1956    2433 
   Size:        553    1058     345     477

  Fragments arranged by size: 

                1058     553     477     345
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1002    2433 
   Size:       1002    1431
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     529     700     997    1020    1462    1732    1750    1834 
   Size:        529     171     297      23     442     270      18      84

Cuts at:   1834    2433 
   Size:        599

  Fragments arranged by size: 

                 599     529     442     297     270     171      84      23
                  18
 
Bpu10I CC'TnA_GC

Cuts at:      0     100     627    1690    2433 
   Size:        100     527    1063     743

  Fragments arranged by size: 

                1063     743     527     100
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     280    1495    2433 
   Size:        280    1215     938

  Fragments arranged by size: 

                1215     938     280
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     733     781    1150    2433 
   Size:        733      48     369    1283

  Fragments arranged by size: 

                1283     733     369      48
 
BsaHI Gr'CG_yC

Cuts at:      0      24     503     865    2433 
   Size:         24     479     362    1568

  Fragments arranged by size: 

                1568     479     362      24
 
BsaWI w'CCGG_w

Cuts at:      0    1262    2433 
   Size:       1262    1171
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     868     898     988    1018    1048    1562    1592    1717 
   Size:        868      30      90      30      30     514      30     125

Cuts at:   1717    1747    2083    2113    2433 
   Size:         30     336      30     320

  Fragments arranged by size: 

                 868     514     336     320     125      90      30      30
                  30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      22      88     739     856     904    1066    1729    1831 
   Size:         22      66     651     117      48     162     663     102

Cuts at:   1831    2182    2433 
   Size:        351     251

  Fragments arranged by size: 

                 663     651     351     251     162     117     102      66
                  48      22
 
BseYI C'CCAG_C

Cuts at:      0     370     445     846     855    1446    1493    2279    2433 
   Size:        370      75     401       9     591      47     786     154

  Fragments arranged by size: 

                 786     591     401     370     154      75      47       9
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2233    2238    2275    2433 
   Size:       2233       5      37     158

  Fragments arranged by size: 

                2233     158      37       5
 
BsiHKAI G_wGCw'C

Cuts at:      0     119    2159    2320    2433 
   Size:        119    2040     161     113

  Fragments arranged by size: 

                2040     161     119     113
 
BsmI GAATG_Cn'

Cuts at:      0     691    2433 
   Size:        691    1742
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     560    2433 
   Size:        560    1873
 
Bsp1286I G_dGCh'C

Cuts at:      0     119     152     185     406     427     550     801    1839 
   Size:        119      33      33     221      21     123     251    1038

Cuts at:   1839    2045    2159    2206    2320    2368    2433 
   Size:        206     114      47     114      48      65

  Fragments arranged by size: 

                1038     251     221     206     123     119     114     114
                  65      48      47      33      33      21
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     559     822    1037    1209    2433 
   Size:        559     263     215     172    1224

  Fragments arranged by size: 

                1224     559     263     215     172
 
BsrBI CCG'CTC

Cuts at:      0    1637    1667    1845    2433 
   Size:       1637      30     178     588

  Fragments arranged by size: 

                1637     588     178      30
 
BsrFI r'CCGG_y

Cuts at:      0     216     317    2433 
   Size:        216     101    2116

  Fragments arranged by size: 

                2116     216     101
 
BssHII G'CGCG_C

Cuts at:      0     333    2433 
   Size:        333    2100
 
BssSI C'ACGA_G

Cuts at:      0    2050    2251    2433 
   Size:       2050     201     182

  Fragments arranged by size: 

                2050     201     182
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1704    2247    2289    2433 
   Size:       1704     543      42     144

  Fragments arranged by size: 

                1704     543     144      42
 
BstEII G'GTnAC_C

Cuts at:      0     276    1458    2433 
   Size:        276    1182     975

  Fragments arranged by size: 

                1182     975     276
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     825    2028    2433 
   Size:        825    1203     405

  Fragments arranged by size: 

                1203     825     405
 
BstYI r'GATC_y

Cuts at:      0    1547    1979    2131    2433 
   Size:       1547     432     152     302

  Fragments arranged by size: 

                1547     432     302     152
 
Bsu36I CC'TnA_GG

Cuts at:      0     722    2433 
   Size:        722    1711
 
BtgI C'CryG_G

Cuts at:      0    2006    2117    2321    2433 
   Size:       2006     111     204     112

  Fragments arranged by size: 

                2006     204     112     111
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     590    1941    2433 
   Size:        590    1351     492

  Fragments arranged by size: 

                1351     590     492
 
BtsI GCAGTG_nn'

Cuts at:      0    1407    1650    2433 
   Size:       1407     243     783

  Fragments arranged by size: 

                1407     783     243
 
DraIII CAC_nnn'GTG

Cuts at:      0    1704    2433 
   Size:       1704     729
 
EaeI y'GGCC_r

Cuts at:      0     931    1596    2103    2306    2433 
   Size:        931     665     507     203     127

  Fragments arranged by size: 

                 931     665     507     203     127
 
EcoICRI GAG'CTC

Cuts at:      0     117    2157    2318    2433 
   Size:        117    2040     161     115

  Fragments arranged by size: 

                2040     161     117     115
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     418     447     529     700     940     997    1020    1462 
   Size:        418      29      82     171     240      57      23     442

Cuts at:   1462    1732    1750    1834    1969    2433 
   Size:        270      18      84     135     464

  Fragments arranged by size: 

                 464     442     418     270     240     171     135      84
                  82      57      29      23      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2251    2433 
   Size:       2251     182
 
EcoO109I rG'GnC_Cy

Cuts at:      0     181     182     402     403     581     797     798    1941 
   Size:        181       1     220       1     178     216       1    1143

Cuts at:   1941    2041    2244    2433 
   Size:        100     203     189

  Fragments arranged by size: 

                1143     220     216     203     189     181     178     100
                   1       1       1
 
EcoRI G'AATT_C

Cuts at:      0    1501    2433 
   Size:       1501     932
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1290    1500    1536    2433 
   Size:       1290     210      36     897

  Fragments arranged by size: 

                1290     897     210      36
 
HaeII r_GCGC'y

Cuts at:      0      27     359    1336    2433 
   Size:         27     332     977    1097

  Fragments arranged by size: 

                1097     977     332      27
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     679     711     868     900    1018    1050    1207    1239 
   Size:        679      32     157      32     118      32     157      32

Cuts at:   1239    1562    1594    1717    1749    2083    2115    2433 
   Size:        323      32     123      32     334      32     318

  Fragments arranged by size: 

                 679     334     323     318     157     157     123     118
                  32      32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0      69    2433 
   Size:         69    2364
 
KasI G'GCGC_C

Cuts at:      0      23    2433 
   Size:         23    2410
 
KpnI G_GTAC'C

Cuts at:      0    1095    2433 
   Size:       1095    1338
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     686    1031    2433 
   Size:        686     345    1402

  Fragments arranged by size: 

                1402     686     345
 
MscI TGG'CCA

Cuts at:      0    1598    2433 
   Size:       1598     835
 
MslI CAynn'nnrTG

Cuts at:      0     208     823    2433 
   Size:        208     615    1610

  Fragments arranged by size: 

                1610     615     208
 
MspA1I CmG'CkG

Cuts at:      0     104     348    1166    1497    1710    1827    1833    1878 
   Size:        104     244     818     331     213     117       6      45

Cuts at:   1878    2073    2129    2409    2433 
   Size:        195      56     280      24

  Fragments arranged by size: 

                 818     331     280     244     213     195     117     104
                  56      45      24       6
 
NarI GG'CG_CC

Cuts at:      0      24    2433 
   Size:         24    2409
 
NsiI A_TGCA'T

Cuts at:      0     693    2433 
   Size:        693    1740
 
PasI CC'CwG_GG

Cuts at:      0     133     170     793    1937    2038    2433 
   Size:        133      37     623    1144     101     395

  Fragments arranged by size: 

                1144     623     395     133     101      37
 
PfoI T'CCnGG_A

Cuts at:      0     836    2433 
   Size:        836    1597
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     988    1020    2433 
   Size:        988      32    1413

  Fragments arranged by size: 

                1413     988      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     581    1941    2433 
   Size:        581    1360     492

  Fragments arranged by size: 

                1360     581     492
 
PspOMI G'GGCC_C

Cuts at:      0     181     402     797    1835    2041    2433 
   Size:        181     221     395    1038     206     392

  Fragments arranged by size: 

                1038     395     392     221     206     181
 
PstI C_TGCA'G

Cuts at:      0    2243    2363    2433 
   Size:       2243     120      70

  Fragments arranged by size: 

                2243     120      70
 
PvuII CAG'CTG

Cuts at:      0     348    1497    1878    2409    2433 
   Size:        348    1149     381     531      24

  Fragments arranged by size: 

                1149     531     381     348      24
 
SacI G_AGCT'C

Cuts at:      0     119    2159    2320    2433 
   Size:        119    2040     161     113

  Fragments arranged by size: 

                2040     161     119     113
 
SalI G'TCGA_C

Cuts at:      0      67    2433 
   Size:         67    2366
 
SanDI GG'GwC_CC

Cuts at:      0    1941    2433 
   Size:       1941     492
 
SfcI C'TryA_G

Cuts at:      0    1062    2239    2359    2433 
   Size:       1062    1177     120      74

  Fragments arranged by size: 

                1177    1062     120      74
 
SfoI GGC'GCC

Cuts at:      0      25    2433 
   Size:         25    2408
 
SmlI C'TyrA_G

Cuts at:      0     295    1510    2433 
   Size:        295    1215     923

  Fragments arranged by size: 

                1215     923     295
 
StuI AGG'CCT

Cuts at:      0      13    2354    2433 
   Size:         13    2341      79

  Fragments arranged by size: 

                2341      79      13
 
StyI C'CwwG_G

Cuts at:      0    1118    2433 
   Size:       1118    1315
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     656    1438    1915    2433 
   Size:        656     782     477     518

  Fragments arranged by size: 

                 782     656     518     477
 
TatI w'GTAC_w

Cuts at:      0     224    2433 
   Size:        224    2209
 
Tth111I GACn'n_nGTC

Cuts at:      0     882    2185    2433 
   Size:        882    1303     248

  Fragments arranged by size: 

                1303     882     248
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     972    1044    2433 
   Size:        972      72    1389

  Fragments arranged by size: 

                1389     972      72
 
XmnI GAAnn'nnTTC

Cuts at:      0    1483    2433 
   Size:       1483     950
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AfeI   AflIII     AleI     AloI
    AlwNI     ApaI     ApoI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspMI    BsrBI    BsrFI   BssHII    BssSI   BstAPI   BstEII    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI   DraIII     EaeI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    HaeII    Hin4I   HincII
     KasI     KpnI     MmeI     MscI     MslI   MspA1I     NarI     NsiI
     PasI     PfoI     PpiI    PpuMI   PspOMI     PstI    PvuII     SacI
     SalI    SanDI     SfcI     SfoI     SmlI     StuI     StyI    TaqII
     TatI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AclI    AflII     AgeI     AhdI     AlfI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI     BcgI    BciVI     BclI     BmtI
    BsaAI    BsaBI    BsiEI    BsiWI    BspEI    BspHI    BsrDI    BsrGI
    BstBI  BstZ17I     ClaI    CspCI     DraI     DrdI     EagI     EarI
     EciI    EcoRV     FseI     FspI    FspAI  HindIII     HpaI     MfeI
     MluI     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NspI     PacI     PciI    PflMI     PmeI     PmlI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII    SacII     SapI     SbfI     ScaI
    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     TstI     XbaI     XhoI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com