Restriction map for pF1KA1475
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# NO CUT ENZYMES (   75 enzymes) #
##################################

AatII       Acc65I      AclI        AflIII      AgeI        
AhdI        AscI        AseI        AsnI        Asp718I     
AspEI       AvrII       BamHI       BcgI        BglII       
BlnI        BsaBI       BsiWI       BspDI       BspHI       
BspLU11I    BsrDI       BssHII      Bst1107I    BstEII      
BstPI       BstZ17I     ClaI        CpoI        DraI        
DrdI        Eam1105I    EcoO65I     EcoRI       EcoRV       
EcoT22I     HincII      HindII      HindIII     HpaI        
KpnI        KspI        MamI        MfeI        MluI        
MunI        NotI        NruI        NsiI        NspI        
PacI        PaeR7I      PflMI       PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        ScaI        SexAI       
SgrAI       SnaBI       SpeI        SphI        SspI        
SwaI        TfiI        Van91I      XbaI        XhoI        


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# ONE CUT ENZYMES (   58 enzymes) #
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AccI         2146  GTCTAC               GT^[AC][GT]AC
AccII        2131  CGCG                 CG^CG
AccIII        555  TCCGGA               T^CCGGA
AcsI         1084  AAATTT               [GA]^AATT[CT]
AflII        2425  CTTAAG               C^TTAAG
Alw44I       1038  GTGCAC               G^TGCAC
AocI         2233  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      2888  AGCGCT               AGC^GCT
ApaLI        1038  GTGCAC               G^TGCAC
ApoI         1084  AAATTT               [GA]^AATT[CT]
AspI         1267  GACACGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        2252  TGCGCA               TGC^GCA
BanI          899  GGCGCC               G^G[CT][GA]CC
BbeI          899  GGCGCC               GGCGC^C
BbsI         1156  GTCTTC               GAAGAC(2/6)
BciVI         219  GGATAC               GTATCC(6/5)
BclI         1682  TGATCA               T^GATCA
BfrI         2425  CTTAAG               C^TTAAG
BlpI          915  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      915  GCTGAGC              GC^T[ACGT]AGC
BpuAI        1156  GTCTTC               GAAGAC(2/6)
BsaI         2504  GAGACC               GGTCTC(1/5)
BsaWI         555  TCCGGA               [AT]^CCGG[AT]
BseAI         555  TCCGGA               T^CCGGA
BsmBI         183  CGTCTC               CGTCTC(1/5)
Bsp1407I     2307  TGTACA               T^GTACA
BspEI         555  TCCGGA               T^CCGGA
BsrGI        2307  TGTACA               T^GTACA
BssSI        1726  CACGAG               CACGAG(-5/-1)
BstBI        1568  TTCGAA               TT^CGAA
BstUI        2131  CGCG                 CG^CG
BstYI         558  GGATCT               [GA]^GATC[CT]
Bsu36I       2233  CCTGAGG              CC^T[ACGT]AGG
CelII         915  GCTGAGC              GC^T[ACGT]AGC
Eco47III     2888  AGCGCT               AGC^GCT
Eco81I       2233  CCTGAGG              CC^T[ACGT]AGG
FbaI         1682  TGATCA               T^GATCA
FseI         2020  GGCCGGCC             GGCCGG^CC
FspI         2252  TGCGCA               TGC^GCA
KasI          899  GGCGCC               G^GCGCC
MflI          558  GGATCT               [GA]^GATC[CT]
MroI          555  TCCGGA               T^CCGGA
MseI         2426  TTAA                 T^TAA
MslI          608  CACCTGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI         2131  CGCG                 CG^CG
NarI          899  GGCGCC               GG^CGCC
NcoI         2318  CCATGG               C^CATGG
NdeI         2614  CATATG               CA^TATG
NheI         2332  GCTAGC               G^CTAGC
NspV         1568  TTCGAA               TT^CGAA
PflFI        1267  GACACGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SapI         1779  GCTCTTC              GCTCTTC(1/4)
SfoI          899  GGCGCC               GGC^GCC
SfuI         1568  TTCGAA               TT^CGAA
SspBI        2307  TGTACA               T^GTACA
Tru9I        2426  TTAA                 T^TAA
Tth111I      1267  GACACGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoII         558  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AcyI            3  GGCGTC               G[GA]^CG[CT]C
              899  GGCGCC              
ApaI          636  GGGCCC               GGGCC^C
             2776  GGGCCC              
Asp700I      1215  GAACCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1561  GAATGCTTTC          
BbvCI        1578  CCTCAGC              CCTCAGC(-5/-2)
              715  GCTGAGG             
BsaHI           3  GGCGTC               G[GA]^CG[CT]C
              899  GGCGCC              
BsgI         2222  GTGCAG               GTGCAG(16/14)
             2290  GTGCAG              
BsmI           99  GAATGC               GAATGC(1/-1)
             1561  GAATGC              
BstXI         260  CCAACCACGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              785  CCAGCAACCTGG        
Hin1I           3  GGCGTC               G[GA]^CG[CT]C
              899  GGCGCC              
PpuMI         359  AGGTCCT              [GA]G^G[AT]CC[CT]
             2153  AGGTCCT             
SacI          517  GAGCTC               GAGCT^C
             1489  GAGCTC              
SbfI         2190  CCTGCAGG             CCTGCA^GG
             2508  CCTGCAGG            
SfiI         1278  GGCCCGGGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             2742  GGCCAAGGAGGCC       
Sse8387I     2190  CCTGCAGG             CCTGCA^GG
             2508  CCTGCAGG            
StuI         1730  AGGCCT               AGG^CCT
             2429  AGGCCT              
Tsp509I      1085  AATT                 ^AATT
             2249  AATT                
XmnI         1215  GAACCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1561  GAATGCTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  108 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AspHI       AvaI        AvaII       BalI        BanII       
BbrPI       BbvI        BcnI        BfaI        BglI        
BmyI        BpmI        BsaAI       BsaJI       BseRI       
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrFI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DraIII      DsaI        
EaeI        EagI        EarI        EclXI       Eco52I      
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MluNI       MnlI        
MscI        MspA1I      MspI        MvaI        MwoI        
NaeI        NciI        NdeII       NgoMIV      NlaIII      
NlaIV       PleI        PmaCI       PmlI        PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmaI        SmlI        StyI        
TaqI        TseI        Tsp45I      TspRI       TthHB8I     
XcmI        XmaCI       XmaI        
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