Restriction map for pF1KA1475
 (Linear) (Six-base) MAPSORT of: KIAA1475.seq  Check: 4198  from: 1  to: 2919



KIAA1475.orf

 With 182 enzymes: SgfI * 

                        September 18, 2009 16:59  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     341    1044    1911    2184    2572    2919 
   Size:        341     703     867     273     388     347

  Fragments arranged by size: 

                 867     703     388     347     341     273
 
AccI GT'mk_AC

Cuts at:      0    2147    2919 
   Size:       2147     772
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1117    2290    2919 
   Size:       1117    1173     629

  Fragments arranged by size: 

                1173    1117     629
 
AfeI AGC'GCT

Cuts at:      0    2890    2919 
   Size:       2890      29
 
AflII C'TTAA_G

Cuts at:      0    2425    2919 
   Size:       2425     494
 
AleI CACnn'nnGTG

Cuts at:      0     612    2919 
   Size:        612    2307
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     630    2919 
   Size:        630    2289
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      19      51    2919 
   Size:         19      32    2868

  Fragments arranged by size: 

                2868      32      19
 
AlwNI CAG_nnn'CTG

Cuts at:      0     610    2454    2517    2919 
   Size:        610    1844      63     402

  Fragments arranged by size: 

                1844     610     402      63
 
ApaI G_GGCC'C

Cuts at:      0     640    2780    2919 
   Size:        640    2140     139

  Fragments arranged by size: 

                2140     640     139
 
ApaLI G'TGCA_C

Cuts at:      0    1038    2919 
   Size:       1038    1881
 
ApoI r'AATT_y

Cuts at:      0    1084    2919 
   Size:       1084    1835
 
AvaI C'yCGr_G

Cuts at:      0      87     842    1184    1280    2738    2919 
   Size:         87     755     342      96    1458     181

  Fragments arranged by size: 

                1458     755     342     181      96      87
 
BanI G'GyrC_C

Cuts at:      0     899    2919 
   Size:        899    2020
 
BanII G_rGCy'C

Cuts at:      0     260     521     640     740    1493    1515    2546    2734 
   Size:        260     261     119     100     753      22    1031     188

Cuts at:   2734    2780    2919 
   Size:         46     139

  Fragments arranged by size: 

                1031     753     261     260     188     139     119     100
                  46      22
 
BbeI G_GCGC'C

Cuts at:      0     903    2919 
   Size:        903    2016
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1149    2919 
   Size:       1149    1770
 
BbvCI CC'TCA_GC

Cuts at:      0     716    1579    2919 
   Size:        716     863    1340

  Fragments arranged by size: 

                1340     863     716
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     213    2919 
   Size:        213    2706
 
BclI T'GATC_A

Cuts at:      0    1682    2919 
   Size:       1682    1237
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1285    1618    2531    2749    2919 
   Size:       1285     333     913     218     170

  Fragments arranged by size: 

                1285     913     333     218     170
 
BlpI GC'TnA_GC

Cuts at:      0     916    2919 
   Size:        916    2003
 
Bme1580I G_kGCm'C

Cuts at:      0     125     457     640    1042    2490    2780    2919 
   Size:        125     332     183     402    1448     290     139

  Fragments arranged by size: 

                1448     402     332     290     183     139     125
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     460     987    2919 
   Size:        460     527    1932

  Fragments arranged by size: 

                1932     527     460
 
BmtI G_CTAG'C

Cuts at:      0    2336    2919 
   Size:       2336     583
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     171    1099    1339    1402    2068    2704    2919 
   Size:        130      41     928     240      63     666     636     215

  Fragments arranged by size: 

                 928     666     636     240     215     130      63      41
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     346     716    1498    1579    2919 
   Size:        346     370     782      81    1340

  Fragments arranged by size: 

                1340     782     370     346      81
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     643    1357    2074    2332    2779    2919 
   Size:        643     714     717     258     447     140

  Fragments arranged by size: 

                 717     714     643     447     258     140
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2498    2919 
   Size:       2498     421
 
BsaAI yAC'GTr

Cuts at:      0     267     369     582    1170    2286    2919 
   Size:        267     102     213     588    1116     633

  Fragments arranged by size: 

                1116     633     588     267     213     102
 
BsaHI Gr'CG_yC

Cuts at:      0       4     900    2919 
   Size:          4     896    2019

  Fragments arranged by size: 

                2019     896       4
 
BsaWI w'CCGG_w

Cuts at:      0     555    2919 
   Size:        555    2364
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1021    1051    2919 
   Size:       1021      30    1868

  Fragments arranged by size: 

                1868    1021      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     937    1107    1375    1411    1753    2185    2251    2701 
   Size:        937     170     268      36     342     432      66     450

Cuts at:   2701    2911    2914    2919 
   Size:        210       3       5

  Fragments arranged by size: 

                 937     450     432     342     268     210     170      66
                  36       5       3
 
BseYI C'CCAG_C

Cuts at:      0     104     273     784    1775    1965    2056    2489    2703 
   Size:        104     169     511     991     190      91     433     214

Cuts at:   2703    2721    2779    2885    2919 
   Size:         18      58     106      34

  Fragments arranged by size: 

                 991     511     433     214     190     169     106     104
                  91      58      34      18
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2243    2311    2919 
   Size:       2243      68     608

  Fragments arranged by size: 

                2243     608      68
 
BsiEI CG_ry'CG

Cuts at:      0      47    2026    2868    2872    2919 
   Size:         47    1979     842       4      47

  Fragments arranged by size: 

                1979     842      47      47       4
 
BsiHKAI G_wGCw'C

Cuts at:      0     521     619    1042    1493    1757    2919 
   Size:        521      98     423     451     264    1162

  Fragments arranged by size: 

                1162     521     451     423     264      98
 
BsmI GAATG_Cn'

Cuts at:      0     105    1567    2919 
   Size:        105    1462    1352

  Fragments arranged by size: 

                1462    1352     105
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     189    2919 
   Size:        189    2730
 
Bsp1286I G_dGCh'C

Cuts at:      0     125     260     457     521     619     640     740    1042 
   Size:        125     135     197      64      98      21     100     302

Cuts at:   1042    1493    1515    1757    2490    2546    2734    2780    2919 
   Size:        451      22     242     733      56     188      46     139

  Fragments arranged by size: 

                 733     451     302     242     197     188     139     135
                 125     100      98      64      56      46      22      21
            
 
BspEI T'CCGG_A

Cuts at:      0     555    2919 
   Size:        555    2364
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     341    1044    1911    2006    2184    2237    2516    2572 
   Size:        341     703     867      95     178      53     279      56

Cuts at:   2572    2919 
   Size:        347

  Fragments arranged by size: 

                 867     703     347     341     279     178      95      56
                  53
 
BsrBI CCG'CTC

Cuts at:      0      41     633    1149    1251    1701    2858    2919 
   Size:         41     592     516     102     450    1157      61

  Fragments arranged by size: 

                1157     592     516     450     102      61      41
 
BsrFI r'CCGG_y

Cuts at:      0    1502    1856    2021    2867    2919 
   Size:       1502     354     165     846      52

  Fragments arranged by size: 

                1502     846     354     165      52
 
BsrGI T'GTAC_A

Cuts at:      0    2307    2919 
   Size:       2307     612
 
BssSI C'ACGA_G

Cuts at:      0    1726    2919 
   Size:       1726    1193
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1059    2289    2517    2646    2919 
   Size:       1059    1230     228     129     273

  Fragments arranged by size: 

                1230    1059     273     228     129
 
BstBI TT'CG_AA

Cuts at:      0    1569    2919 
   Size:       1569    1350
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     267     792    2919 
   Size:        267     525    2127

  Fragments arranged by size: 

                2127     525     267
 
BstYI r'GATC_y

Cuts at:      0     558    2919 
   Size:        558    2361
 
Bsu36I CC'TnA_GG

Cuts at:      0    2234    2919 
   Size:       2234     685
 
BtgI C'CryG_G

Cuts at:      0    2016    2177    2318    2768    2919 
   Size:       2016     161     141     450     151

  Fragments arranged by size: 

                2016     450     161     151     141
 
BtsI GCAGTG_nn'

Cuts at:      0     223    1040    1465    1685    2224    2919 
   Size:        223     817     425     220     539     695

  Fragments arranged by size: 

                 817     695     539     425     223     220
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2116    2151    2919 
   Size:       2116      35     768

  Fragments arranged by size: 

                2116     768      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1107    1668    2076    2289    2919 
   Size:       1107     561     408     213     630

  Fragments arranged by size: 

                1107     630     561     408     213
 
EaeI y'GGCC_r

Cuts at:      0      44     394    1229    1430    1748    2019    2023    2121 
   Size:         44     350     835     201     318     271       4      98

Cuts at:   2121    2210    2610    2819    2869    2919 
   Size:         89     400     209      50      50

  Fragments arranged by size: 

                 835     400     350     318     271     209     201      98
                  89      50      50      44       4
 
EagI C'GGCC_G

Cuts at:      0      44    2023    2869    2919 
   Size:         44    1979     846      50

  Fragments arranged by size: 

                1979     846      50      44
 
EarI CTCTTCn'nnn_

Cuts at:      0     688    1786    2291    2465    2605    2919 
   Size:        688    1098     505     174     140     314

  Fragments arranged by size: 

                1098     688     505     314     174     140
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     559    2919 
   Size:        559    2360
 
EcoICRI GAG'CTC

Cuts at:      0     519    1491    2919 
   Size:        519     972    1428

  Fragments arranged by size: 

                1428     972     519
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     171    1099    1117    1339    1402    2068    2290 
   Size:        130      41     928      18     222      63     666     222

Cuts at:   2290    2704    2919 
   Size:        414     215

  Fragments arranged by size: 

                 928     666     414     222     222     215     130      63
                  41      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     214    2233    2638    2919 
   Size:        214    2019     405     281

  Fragments arranged by size: 

                2019     405     281     214
 
EcoO109I rG'GnC_Cy

Cuts at:      0     360     472     636     637     955    1533    1743    2154 
   Size:        360     112     164       1     318     578     210     411

Cuts at:   2154    2776    2919 
   Size:        622     143

  Fragments arranged by size: 

                 622     578     411     360     318     210     164     143
                 112       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     556    1581    2919 
   Size:        556    1025    1338

  Fragments arranged by size: 

                1338    1025     556
 
FseI GG_CCGG'CC

Cuts at:      0    2025    2919 
   Size:       2025     894
 
FspI TGC'GCA

Cuts at:      0    2254    2919 
   Size:       2254     665
 
HaeII r_GCGC'y

Cuts at:      0     681     903    2892    2919 
   Size:        681     222    1989      27

  Fragments arranged by size: 

                1989     681     222      27
 
KasI G'GCGC_C

Cuts at:      0     899    2919 
   Size:        899    2020
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     418    2919 
   Size:        418    2501
 
MscI TGG'CCA

Cuts at:      0    1231    1432    2123    2212    2612    2919 
   Size:       1231     201     691      89     400     307

  Fragments arranged by size: 

                1231     691     400     307     201      89
 
MslI CAynn'nnrTG

Cuts at:      0     612    2919 
   Size:        612    2307
 
MspA1I CmG'CkG

Cuts at:      0     108     198     420    1095    1131    2460    2824    2919 
   Size:        108      90     222     675      36    1329     364      95

  Fragments arranged by size: 

                1329     675     364     222     108      95      90      36
            
 
NaeI GCC'GGC

Cuts at:      0    1504    1858    2023    2919 
   Size:       1504     354     165     896

  Fragments arranged by size: 

                1504     896     354     165
 
NarI GG'CG_CC

Cuts at:      0     900    2919 
   Size:        900    2019
 
NcoI C'CATG_G

Cuts at:      0    2318    2919 
   Size:       2318     601
 
NdeI CA'TA_TG

Cuts at:      0    2615    2919 
   Size:       2615     304
 
NgoMIV G'CCGG_C

Cuts at:      0    1502    1856    2021    2919 
   Size:       1502     354     165     898

  Fragments arranged by size: 

                1502     898     354     165
 
NheI G'CTAG_C

Cuts at:      0    2332    2919 
   Size:       2332     587
 
PasI CC'CwG_GG

Cuts at:      0     723    2919 
   Size:        723    2196
 
PfoI T'CCnGG_A

Cuts at:      0      11    1076    1379    2447    2919 
   Size:         11    1065     303    1068     472

  Fragments arranged by size: 

                1068    1065     472     303      11
 
PmlI CAC'GTG

Cuts at:      0     267    1170    2286    2919 
   Size:        267     903    1116     633

  Fragments arranged by size: 

                1116     903     633     267
 
PpuMI rG'GwC_Cy

Cuts at:      0     360    2154    2919 
   Size:        360    1794     765

  Fragments arranged by size: 

                1794     765     360
 
PspOMI G'GGCC_C

Cuts at:      0     636    2776    2919 
   Size:        636    2140     143

  Fragments arranged by size: 

                2140     636     143
 
PstI C_TGCA'G

Cuts at:      0    1421    1520    2195    2419    2459    2513    2642    2696 
   Size:       1421      99     675     224      40      54     129      54

Cuts at:   2696    2919 
   Size:        223

  Fragments arranged by size: 

                1421     675     224     223     129      99      54      54
                  40
 
PvuII CAG'CTG

Cuts at:      0     108     198     420    1131    2919 
   Size:        108      90     222     711    1788

  Fragments arranged by size: 

                1788     711     222     108      90
 
SacI G_AGCT'C

Cuts at:      0     521    1493    2919 
   Size:        521     972    1426

  Fragments arranged by size: 

                1426     972     521
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1786    2919 
   Size:       1786    1133
 
SbfI CC_TGCA'GG

Cuts at:      0    2195    2513    2919 
   Size:       2195     318     406

  Fragments arranged by size: 

                2195     406     318
 
SfcI C'TryA_G

Cuts at:      0     798    1417    1516    1896    2191    2415    2455    2477 
   Size:        798     619      99     380     295     224      40      22

Cuts at:   2477    2509    2638    2692    2919 
   Size:         32     129      54     227

  Fragments arranged by size: 

                 798     619     380     295     227     224     129      99
                  54      40      32      22
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1285    2749    2919 
   Size:       1285    1464     170

  Fragments arranged by size: 

                1464    1285     170
 
SfoI GGC'GCC

Cuts at:      0     901    2919 
   Size:        901    2018
 
SmaI CCC'GGG

Cuts at:      0     844    1282    2740    2919 
   Size:        844     438    1458     179

  Fragments arranged by size: 

                1458     844     438     179
 
SmlI C'TyrA_G

Cuts at:      0     622    1372    2089    2347    2425    2794    2919 
   Size:        622     750     717     258      78     369     125

  Fragments arranged by size: 

                 750     717     622     369     258     125      78
 
SrfI GCCC'GGGC

Cuts at:      0    2740    2919 
   Size:       2740     179
 
StuI AGG'CCT

Cuts at:      0    1732    2431    2919 
   Size:       1732     699     488

  Fragments arranged by size: 

                1732     699     488
 
StyI C'CwwG_G

Cuts at:      0     495    1556    2318    2726    2744    2919 
   Size:        495    1061     762     408      18     175

  Fragments arranged by size: 

                1061     762     495     408     175      18
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     776    1812    2919 
   Size:        776    1036    1107

  Fragments arranged by size: 

                1107    1036     776
 
TatI w'GTAC_w

Cuts at:      0    1670    2307    2834    2919 
   Size:       1670     637     527      85

  Fragments arranged by size: 

                1670     637     527      85
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     755     787    1021    1053    2919 
   Size:        755      32     234      32    1866

  Fragments arranged by size: 

                1866     755     234      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1270    2919 
   Size:       1270    1649
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     404    1941    2325    2919 
   Size:        404    1537     384     594

  Fragments arranged by size: 

                1537     594     404     384
 
XmaI C'CCGG_G

Cuts at:      0     842    1280    2738    2919 
   Size:        842     438    1458     181

  Fragments arranged by size: 

                1458     842     438     181
 
XmnI GAAnn'nnTTC

Cuts at:      0    1219    1565    2919 
   Size:       1219     346    1354

  Fragments arranged by size: 

                1354    1219     346
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI    AflII     AleI     AlfI     AloI
    AlwNI     ApaI    ApaLI     ApoI     AvaI     BanI    BanII     BbeI
     BbsI    BbvCI    BciVI     BclI     BglI     BlpI Bme1580I     BmrI
     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspMI    BsrBI    BsrFI    BsrGI    BssSI   BstAPI
    BstBI    BstXI    BstYI   Bsu36I     BtgI     BtsI    CspCI   DraIII
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
     FalI     FseI     FspI    HaeII     KasI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     PasI
     PfoI     PmlI    PpuMI   PspOMI     PstI    PvuII     SacI     SapI
     SbfI     SfcI     SfiI     SfoI     SmaI     SmlI     SrfI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI   AflIII     AgeI     AhdI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BcgI    BfrBI    BglII    BmgBI
     BplI    BsaBI    BsiWI    BspHI    BsrDI   BssHII   BstEII  BstZ17I
    BtgZI     ClaI     DraI     DrdI    EcoRI    EcoRV    FspAI    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     NotI     NruI
     NsiI     NspI     PacI     PciI    PflMI     PmeI     PpiI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     ScaI    SexAI     SgfI    SgrAI    SnaBI     SpeI     SphI     SspI
     SwaI     XbaI     XhoI     ZraI
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