Restriction map for pF1KA1370
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# NO CUT ENZYMES (  120 enzymes) #
##################################

Acc65I      AccII       AccIII      AclI        AflII       
AgeI        Alw44I      AocI        Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp718I     AspI        
AvaI        AviII       BalI        BamHI       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BfrI        BglI        BsaBI       BsaWI       BseAI       
BsiEI       BsmI        BsoBI       BspDI       BspEI       
BspMI       BsrFI       BssHII      BssSI       Bst1107I    
BstBI       BstUI       BstXI       BstZ17I     Bsu36I      
CfoI        Cfr10I      ClaI        CpoI        DraII       
DraIII      DrdI        EaeI        EagI        EclXI       
Eco47III    Eco52I      Eco81I      EcoO109I    EcoRV       
FauI        FbaI        FseI        FspI        HaeII       
HhaI        HinP1I      HincII      HindII      HpaI        
KasI        KpnI        KspI        MamI        MluI        
MluNI       MroI        MscI        MvnI        NaeI        
NarI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SpeI        SphI        Sse8387I    SwaI        
Tth111I     XcmI        XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   38 enzymes) #
###################################

AatII        1178  GACGTC               GACGT^C
AcyI         1178  GACGTC               G[GA]^CG[CT]C
AhdI         2302  GACTGTTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI          310  GGGCCC               GGGCC^C
AspEI        2302  GACTGTTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII         818  GGACC                G^G[AT]CC
AvrII        2634  CCTAGG               C^CTAGG
BanI          825  GGCACC               G^G[CT][GA]CC
BanII         310  GGGCCC               G[GA]GC[CT]^C
BglII        3101  AGATCT               A^GATCT
BlnI         2634  CCTAGG               C^CTAGG
BlpI         1468  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1468  GCTCAGC              GC^T[ACGT]AGC
BsaAI        1788  TACGTA               [CT]AC^GT[GA]
BsaHI        1178  GACGTC               G[GA]^CG[CT]C
BsaI           60  GGTCTC               GGTCTC(1/5)
BsgI          678  GTGCAG               GTGCAG(16/14)
BsiWI        3120  CGTACG               C^GTACG
BsmBI        3116  GAGACG               CGTCTC(1/5)
Bsp1407I     1954  TGTACA               T^GTACA
BspHI        2580  TCATGA               T^CATGA
BsrGI        1954  TGTACA               T^GTACA
BstEII        409  GGTGACC              G^GT[ACGT]ACC
BstPI         409  GGTGACC              G^GT[ACGT]ACC
CelII        1468  GCTCAGC              GC^T[ACGT]AGC
Eam1105I     2302  GACTGTTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I       409  GGTGACC              G^GT[ACGT]ACC
EcoRI         716  GAATTC               G^AATTC
Hin1I        1178  GACGTC               G[GA]^CG[CT]C
MfeI          578  CAATTG               C^AATTG
MunI          578  CAATTG               C^AATTG
PflMI         904  CCAGGCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          875  CTGCAG               CTGCA^G
RcaI         2580  TCATGA               T^CATGA
SnaBI        1788  TACGTA               TAC^GTA
SspBI        1954  TGTACA               T^GTACA
StuI         2128  AGGCCT               AGG^CCT
Van91I        904  CCAGGCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccI          547  GTAGAC               GT^[AC][GT]AC
             2310  GTCTAC              
AlwNI         793  CAGCGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
              905  CAGGCACTG           
AspHI        1480  GAGCAC               G[AT]GC[AT]^C
             2606  GTGCTC              
BcnI         1227  CCGGG                CC^[GC]GG
             1511  CCCGG               
BseRI        1352  CTCCTC               GAGGAG(10/8)
               49  GAGGAG              
BsiHKAI      1480  GAGCAC               G[AT]GC[AT]^C
             2606  GTGCTC              
BsmFI         931  GGGAC                GGGAC(10/14)
             2826  GTCCC               
BstNI         904  CCAGG                CC^[AT]GG
             1437  CCAGG               
Cac8I         192  GCTGGC               GC[ACGT]^[ACGT]GC
             2943  GCCAGC              
DraI         1005  TTTAAA               TTT^AAA
             1702  TTTAAA              
DsaI          821  CCATGG               C^C[GA][CT]GG
              927  CCATGG              
EcoRII        904  CCAGG                ^CC[AT]GG
             1437  CCAGG               
EcoT22I       359  ATGCAT               ATGCA^T
             1528  ATGCAT              
HapII        1227  CCGG                 C^CGG
             1512  CCGG                
HgaI          432  GACGC                GACGC(5/10)
              795  GCGTC               
HpaII        1227  CCGG                 C^CGG
             1512  CCGG                
MspA1I        878  CAGCTG               C[AC]G^C[GT]G
             1064  CAGCTG              
MspI         1227  CCGG                 C^CGG
             1512  CCGG                
MvaI          904  CCAGG                CC^[AT]GG
             1437  CCAGG               
NciI         1227  CCGGG                CC^[GC]GG
             1511  CCCGG               
NcoI          821  CCATGG               C^CATGG
              927  CCATGG              
NsiI          359  ATGCAT               ATGCA^T
             1528  ATGCAT              
PvuII         878  CAGCTG               CAG^CTG
             1064  CAGCTG              
SmlI          938  CTCAAG               C^T[CT][GA]AG
             1193  CTCAAG              
XbaI         2533  TCTAGA               T^CTAGA
             3136  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   75 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        ApoI        Asp700I     BbsI        BbvI        
BfaI        BmyI        BpmI        BpuAI       BsaJI       
BsiYI       BslI        BsmAI       Bsp1286I    BspLU11I    
BsrBI       BsrDI       BsrI        BssKI       BstAPI      
BstF5I      BstYI       Cfr13I      DdeI        DpnII       
EarI        EcoNI       EcoT14I     Fnu4HI      FokI        
HaeIII      HindIII     HinfI       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MwoI        NdeI        NdeII       NlaIII      NlaIV       
NspI        PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SspI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       XmnI        
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