Restriction map for pF1KA1343
##################################
# NO CUT ENZYMES (  133 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AflII       AflIII      AlwNI       Aor51HI     ApaI        
AscI        Asp718I     AspI        AvaI        AviII       
AvrII       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BclI        BfrI        BglI        
BglII       BlnI        BpuAI       BsaAI       BsiEI       
BsiWI       BsmBI       BsoBI       Bsp1407I    BspDI       
BspHI       BspLU11I    BspMI       BsrBI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
CfoI        ClaI        CpoI        DraII       DraIII      
DrdI        EagI        EclXI       Eco47III    Eco52I      
EcoNI       EcoO109I    EcoO65I     EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HhaI        HinP1I      HindIII     HpaI        
KasI        KpnI        KspI        MluI        MslI        
MspA1I      MvnI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       StuI        StyI        
SwaI        TaqI        Tth111I     TthHB8I     XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AccIII        255  TCCGGA               T^CCGGA
AciI          906  CCGC                 CCGC(-3/-1)
AcyI          995  GGCGTC               G[GA]^CG[CT]C
AfaI           60  GTAC                 GT^AC
AgeI         1407  ACCGGT               A^CCGGT
AhdI         1167  GACACTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        791  GTGCAC               G^TGCAC
ApaLI         791  GTGCAC               G^TGCAC
AseI          856  ATTAAT               AT^TAAT
AsnI          856  ATTAAT               AT^TAAT
Asp700I       972  GAAGAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1167  GACACTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI          963  TGGCCA               TGG^CCA
BamHI          29  GGATCC               G^GATCC
BcgI         1308  GCAGCAGGCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         110  GTATCC               GTATCC(6/5)
BlpI          775  GCTAAGC              GC^T[ACGT]AGC
BpmI         1303  CTCCAG               CTGGAG(16/14)
Bpu1102I      775  GCTAAGC              GC^T[ACGT]AGC
BsaBI         514  GATTATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         995  GGCGTC               G[GA]^CG[CT]C
BsaI           95  GGTCTC               GGTCTC(1/5)
BseAI         255  TCCGGA               T^CCGGA
BsgI         1480  CTGCAC               GTGCAG(16/14)
BsmI          771  GAATGC               GAATGC(1/-1)
BspEI         255  TCCGGA               T^CCGGA
BsrDI         138  CATTGC               GCAATG(2/0)
BstYI          29  GGATCC               [GA]^GATC[CT]
CelII         775  GCTAAGC              GC^T[ACGT]AGC
DraI         1341  TTTAAA               TTT^AAA
DsaI         1418  CCACGG               C^C[GA][CT]GG
Eam1105I     1167  GACACTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HgaI          996  GCGTC                GACGC(5/10)
Hin1I         995  GGCGTC               G[GA]^CG[CT]C
HincII       1430  GTCAAC               GT[CT]^[GA]AC
HindII       1430  GTCAAC               GT[CT]^[GA]AC
MaeII         759  ACGT                 A^CGT
MaeIII        461  GTGAC                ^GT[ACGT]AC
MamI          514  GATTATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI           29  GGATCC               [GA]^GATC[CT]
MluNI         963  TGGCCA               TGG^CCA
MroI          255  TCCGGA               T^CCGGA
MscI          963  TGGCCA               TGG^CCA
PflMI         493  CCAATGGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        1407  ACCGGT               A^CCGGT
PshBI         856  ATTAAT               AT^TAAT
RsaI           60  GTAC                 GT^AC
SspI          374  AATATT               AAT^ATT
Tsp45I        461  GTGAC                ^GT[GC]AC
Van91I        493  CCAATGGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1050  CCAGGCTTCTAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII          29  GGATCC               [GA]^GATC[CT]
XmnI          972  GAAGAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AocI          652  CCTAAGG              CC^T[ACGT]AGG
              723  CCTGAGG             
AspHI         791  GTGCAC               G[AT]GC[AT]^C
             1122  GAGCAC              
AvaII         869  GGACC                G^G[AT]CC
             1174  GGTCC               
BcnI         1275  CCCGG                CC^[GC]GG
             1331  CCCGG               
BmyI          791  GTGCAC               G[AGT]GC[ACT]^C
             1122  GAGCAC              
BsaWI         255  TCCGGA               [AT]^CCGG[AT]
             1407  ACCGGT              
BsiHKAI       791  GTGCAC               G[AT]GC[AT]^C
             1122  GAGCAC              
BsmAI        1087  GAGAC                GTCTC(1/5)
               96  GTCTC               
BsmFI         222  GTCCC                GGGAC(10/14)
              701  GTCCC               
Bsp1286I      791  GTGCAC               G[AGT]GC[ACT]^C
             1122  GAGCAC              
BsrFI        1286  ACCGGC               [GA]^CCGG[CT]
             1407  ACCGGT              
BsrI          732  ACTGG                ACTGG(1/-1)
             1170  ACTGG               
Bsu36I        652  CCTAAGG              CC^T[ACGT]AGG
              723  CCTGAGG             
Cfr10I       1286  ACCGGC               [GA]^CCGG[CT]
             1407  ACCGGT              
Cfr13I        869  GGACC                G^G[ACGT]CC
             1174  GGTCC               
EaeI          963  TGGCCA               [CT]^GGCC[GA]
             1333  CGGCCA              
Eco81I        652  CCTAAGG              CC^T[ACGT]AGG
              723  CCTGAGG             
EcoRI         826  GAATTC               G^AATTC
             1454  GAATTC              
HaeIII        964  GGCC                 GG^CC
             1334  GGCC                
MfeI          143  CAATTG               C^AATTG
              356  CAATTG              
MunI          143  CAATTG               C^AATTG
              356  CAATTG              
MwoI         1216  GCCCCTATTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             1270  GCTGCCCCGGC         
NciI         1275  CCCGG                CC^[GC]GG
             1331  CCCGG               
NspI          188  GCATGT               [GA]CATG^[CT]
              659  GCATGT              
PleI            5  GAGTC                GAGTC(4/5)
              841  GAGTC               
SapI         1119  GAAGAGC              GCTCTTC(1/4)
             1279  GCTCTTC             
Sau96I        869  GGACC                G^G[ACGT]CC
             1174  GGTCC               
SfcI          696  CTGTAG               C^T[GA][CT]AG
              990  CTACAG              
TfiI          330  GATTC                G^A[AT]TC
             1465  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   43 enzymes) #
#########################################

AcsI        AluI        AlwI        ApoI        BbvI        
BfaI        BsaJI       BseRI       BsiYI       BslI        
BssKI       BstF5I      BstNI       Cac8I       DdeI        
DpnII       EarI        EcoRII      Fnu4HI      FokI        
HapII       HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MboI        MboII       MnlI        
MseI        MspI        MvaI        NdeII       NlaIII      
NlaIV       Sau3AI      ScrFI       SfaNI       Tru9I       
TseI        Tsp509I     TspRI       
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