Restriction map for pF1KA1343
 (Linear) (Six-base) MAPSORT of: KIAA1343.seq  Check: 2742  from: 1  to: 1485



KIAA1343.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:55  ..

AgeI A'CCGG_T

Cuts at:      0    1407    1485 
   Size:       1407      78
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1172    1485 
   Size:       1172     313
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     548     580    1485 
   Size:        548      32     905

  Fragments arranged by size: 

                 905     548      32
 
ApaLI G'TGCA_C

Cuts at:      0     791    1485 
   Size:        791     694
 
ApoI r'AATT_y

Cuts at:      0      37     826    1454    1485 
   Size:         37     789     628      31

  Fragments arranged by size: 

                 789     628      37      31
 
AseI AT'TA_AT

Cuts at:      0     857    1485 
   Size:        857     628
 
BamHI G'GATC_C

Cuts at:      0      29    1485 
   Size:         29    1456
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1297    1331    1485 
   Size:       1297      34     154

  Fragments arranged by size: 

                1297     154      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     121    1485 
   Size:        121    1364
 
BlpI GC'TnA_GC

Cuts at:      0     776    1485 
   Size:        776     709
 
Bme1580I G_kGCm'C

Cuts at:      0     795    1485 
   Size:        795     690
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1180    1485 
   Size:       1180     305
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     765    1485 
   Size:        765     720
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1288    1485 
   Size:       1288     197
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     101    1485 
   Size:        101    1384
 
BsaBI GATnn'nnATC

Cuts at:      0     518    1485 
   Size:        518     967
 
BsaHI Gr'CG_yC

Cuts at:      0     996    1485 
   Size:        996     489
 
BsaWI w'CCGG_w

Cuts at:      0     255    1407    1485 
   Size:        255    1152      78

  Fragments arranged by size: 

                1152     255      78
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     892    1027    1099    1151    1153    1286    1485 
   Size:        892     135      72      52       2     133     199

  Fragments arranged by size: 

                 892     199     135     133      72      52       2
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1465    1485 
   Size:       1465      20
 
BsiHKAI G_wGCw'C

Cuts at:      0     795    1126    1485 
   Size:        795     331     359

  Fragments arranged by size: 

                 795     359     331
 
BsmI GAATG_Cn'

Cuts at:      0     777    1485 
   Size:        777     708
 
Bsp1286I G_dGCh'C

Cuts at:      0     795    1126    1485 
   Size:        795     331     359

  Fragments arranged by size: 

                 795     359     331
 
BspEI T'CCGG_A

Cuts at:      0     255    1485 
   Size:        255    1230
 
BsrDI GCAATG_nn'

Cuts at:      0     137    1485 
   Size:        137    1348
 
BsrFI r'CCGG_y

Cuts at:      0    1286    1407    1485 
   Size:       1286     121      78

  Fragments arranged by size: 

                1286     121      78
 
BstYI r'GATC_y

Cuts at:      0      29    1485 
   Size:         29    1456
 
Bsu36I CC'TnA_GG

Cuts at:      0     653     724    1485 
   Size:        653      71     761

  Fragments arranged by size: 

                 761     653      71
 
BtgI C'CryG_G

Cuts at:      0    1418    1485 
   Size:       1418      67
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     601    1485 
   Size:        601     884
 
BtsI GCAGTG_nn'

Cuts at:      0     899    1261    1477    1485 
   Size:        899     362     216       8

  Fragments arranged by size: 

                 899     362     216       8
 
DraI TTT'AAA

Cuts at:      0    1343    1485 
   Size:       1343     142
 
EaeI y'GGCC_r

Cuts at:      0     963    1333    1485 
   Size:        963     370     152

  Fragments arranged by size: 

                 963     370     152
 
EarI CTCTTCn'nnn_

Cuts at:      0     588    1114    1130    1286    1485 
   Size:        588     526      16     156     199

  Fragments arranged by size: 

                 588     526     199     156      16
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1288    1485 
   Size:       1288     197
 
EcoRI G'AATT_C

Cuts at:      0     826    1454    1485 
   Size:        826     628      31

  Fragments arranged by size: 

                 826     628      31
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     996    1485 
   Size:        996     489
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1158    1190    1456    1485 
   Size:       1158      32     266      29

  Fragments arranged by size: 

                1158     266      32      29
 
HincII GTy'rAC

Cuts at:      0    1432    1485 
   Size:       1432      53
 
MfeI C'AATT_G

Cuts at:      0     143     356    1485 
   Size:        143     213    1129

  Fragments arranged by size: 

                1129     213     143
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     847    1228    1282    1485 
   Size:        847     381      54     203

  Fragments arranged by size: 

                 847     381     203      54
 
MscI TGG'CCA

Cuts at:      0     965    1485 
   Size:        965     520
 
NspI r_CATG'y

Cuts at:      0     192     663    1485 
   Size:        192     471     822

  Fragments arranged by size: 

                 822     471     192
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     499    1485 
   Size:        499     986
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1114    1286    1485 
   Size:       1114     172     199

  Fragments arranged by size: 

                1114     199     172
 
SfcI C'TryA_G

Cuts at:      0     696     990    1485 
   Size:        696     294     495

  Fragments arranged by size: 

                 696     495     294
 
SspI AAT'ATT

Cuts at:      0     376    1485 
   Size:        376    1109
 
TatI w'GTAC_w

Cuts at:      0      59    1485 
   Size:         59    1426
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     237     269     495     527    1485 
   Size:        237      32     226      32     958

  Fragments arranged by size: 

                 958     237     226      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1057    1485 
   Size:       1057     428
 
XmnI GAAnn'nnTTC

Cuts at:      0     976    1485 
   Size:        976     509
 

 Enzymes that do cut:

     AgeI     AhdI     AloI    ApaLI     ApoI     AseI    BamHI     BcgI
    BciVI     BlpI Bme1580I     BmrI     BplI     BpmI     BsaI    BsaBI
    BsaHI    BsaWI    BseRI     BsgI  BsiHKAI     BsmI Bsp1286I    BspEI
    BsrDI    BsrFI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI
     EaeI     EarI  Eco57MI    EcoRI     FalI    Hin4I   HincII     MfeI
     MmeI     MscI     NspI    PflMI     SapI     SfcI     SspI     TatI
     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AleI     AlfI    AlwNI     ApaI     AscI    AsiSI     AvaI
    AvrII     BaeI     BanI    BanII     BbeI     BbsI    BbvCI     BclI
    BfrBI     BglI    BglII    BmgBI     BmtI   Bpu10I    BpuEI    BsaAI
    BsaXI    BseYI    BsiEI    BsiWI    BsmBI    BspHI    BspMI    BsrBI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
     ClaI    CspCI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRV     FseI     FspI    FspAI    HaeII  HindIII     HpaI
     KasI     KpnI     MluI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     StuI     StyI     SwaI    TaqII  Tth111I     XbaI     XhoI     XmaI
     ZraI
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