Restriction map for pF1KA1041
##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AhdI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanII       BbeI        BbrPI       BbsI        
BcgI        BcnI        BfrI        BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaHI       BseAI       
BsgI        BsiWI       BsmBI       BsoBI       BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BssHII      BssSI       Bst1107I    BstBI       
BstUI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DraIII      DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoRV       EcoT14I     FauI        FseI        
FspI        HaeII       HgaI        Hin1I       HindIII     
KasI        KpnI        KspI        MluI        MluNI       
MroI        MscI        MspA1I      MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NspI        NspV        
PacI        PaeR7I      PflFI       PleI        PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SfiI        SfoI        SfuI        SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
StyI        SwaI        Tth111I     XbaI        XhoI        
XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   56 enzymes) #
###################################

AccI         1432  GTAGAC               GT^[AC][GT]AC
AgeI          756  ACCGGT               A^CCGGT
AseI          271  ATTAAT               AT^TAAT
AsnI          271  ATTAAT               AT^TAAT
AspHI        1014  GAGCAC               G[AT]GC[AT]^C
BbvCI         799  CCTCAGC              CCTCAGC(-5/-2)
BciVI        1336  GTATCC               GTATCC(6/5)
BclI          648  TGATCA               T^GATCA
BfaI         1664  CTAG                 C^TAG
BglI          489  GCCTACTCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI         939  GATGAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1101  GGTCTC               GGTCTC(1/5)
BsaWI         756  ACCGGT               [AT]^CCGG[AT]
BseRI         797  CTCCTC               GAGGAG(10/8)
BsiEI        1156  CGACCG               CG[GA][CT]^CG
BsiHKAI      1014  GAGCAC               G[AT]GC[AT]^C
BsmI          168  GAATGC               GAATGC(1/-1)
Bsp1407I     1652  TGTACA               T^GTACA
BsrFI         756  ACCGGT               [GA]^CCGG[CT]
BsrGI        1652  TGTACA               T^GTACA
BstAPI       1779  GCACATGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII         86  GGTTACC              G^GT[ACGT]ACC
BstPI          86  GGTTACC              G^GT[ACGT]ACC
BstXI        1629  CCAACACATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI          484  GCGC                 GCG^C
Cfr10I        756  ACCGGT               [GA]^CCGG[CT]
DraII         445  GGGTCCT              [GA]G^G[ACGT]CC[CT]
DrdI           14  GACAGGCTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          496  CGGCCA               [CT]^GGCC[GA]
EarI          504  GAAGAG               CTCTTC(1/4)
EcoO109I      445  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I        86  GGTTACC              G^GT[ACGT]ACC
EcoRI         369  GAATTC               G^AATTC
EcoT22I      1609  ATGCAT               ATGCA^T
FbaI          648  TGATCA               T^GATCA
HapII         757  CCGG                 C^CGG
HhaI          484  GCGC                 GCG^C
HinP1I        484  GCGC                 G^CGC
HpaI         1302  GTTAAC               GTT^AAC
HpaII         757  CCGG                 C^CGG
Ksp632I       504  GAAGAG               CTCTTC(1/4)
MaeI         1664  CTAG                 C^TAG
MamI          939  GATGAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1859  CAATTG               C^AATTG
MspI          757  CCGG                 C^CGG
MunI         1859  CAATTG               C^AATTG
NsiI         1609  ATGCAT               ATGCA^T
PinAI         756  ACCGGT               A^CCGGT
PpuMI         445  GGGTCCT              [GA]G^G[AT]CC[CT]
PshBI         271  ATTAAT               AT^TAAT
SexAI        1502  ACCAGGT              A^CC[AT]GGT
SfcI          518  CTGTAG               C^T[GA][CT]AG
SgrAI         755  CACCGGTG             C[GA]^CCGG[CT]G
SmlI         1483  CTCAAG               C^T[CT][GA]AG
SspBI        1652  TGTACA               T^GTACA
XcmI         1152  CCATCGACCGCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AvaII         198  GGACC                G^G[AT]CC
              446  GGTCC               
BanI         1314  GGCACC               G^G[CT][GA]CC
             1603  GGTGCC              
BglII         870  AGATCT               A^GATCT
             1479  AGATCT              
BmyI          509  GGGCAC               G[AGT]GC[ACT]^C
             1014  GAGCAC              
BsaJI         435  CCCTGG               C^C[ACGT][ACGT]GG
             1698  CCCTGG              
BsmFI         969  GTCCC                GGGAC(10/14)
             1233  GTCCC               
Bsp1286I      509  GGGCAC               G[AGT]GC[ACT]^C
             1014  GAGCAC              
BstYI         870  AGATCT               [GA]^GATC[CT]
             1479  AGATCT              
HincII        926  GTCAAC               GT[CT]^[GA]AC
             1302  GTTAAC              
HindII        926  GTCAAC               GT[CT]^[GA]AC
             1302  GTTAAC              
MaeII          73  ACGT                 A^CGT
             1300  ACGT                
MflI          870  AGATCT               [GA]^GATC[CT]
             1479  AGATCT              
MslI          220  CACAAAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1057  CATGGCAGTG          
PflMI         702  CCAGAGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1693  CCACCCCCTGG         
Van91I        702  CCAGAGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1693  CCACCCCCTGG         
XhoII         870  AGATCT               [GA]^GATC[CT]
             1479  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
BbvI        BpmI        BsiYI       BslI        BsmAI       
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DpnII       EcoRII      Fnu4HI      
FokI        HaeIII      HinfI       HphI        ItaI        
MaeIII      MboI        MboII       MnlI        MseI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     
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