Restriction map for pF1KA1041
 (Linear) (Six-base) MAPSORT of: KIAA1041.seq  Check: 2436  from: 1  to: 1866



KIAA1041.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:55  ..

AccI GT'mk_AC

Cuts at:      0    1433    1866 
   Size:       1433     433
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1667    1866 
   Size:       1667     199
 
AgeI A'CCGG_T

Cuts at:      0     756    1866 
   Size:        756    1110
 
ApoI r'AATT_y

Cuts at:      0     155     308     369    1647    1866 
   Size:        155     153      61    1278     219

  Fragments arranged by size: 

                1278     219     155     153      61
 
AseI AT'TA_AT

Cuts at:      0     272    1866 
   Size:        272    1594
 
BanI G'GyrC_C

Cuts at:      0    1314    1603    1866 
   Size:       1314     289     263

  Fragments arranged by size: 

                1314     289     263
 
BbvCI CC'TCA_GC

Cuts at:      0     800    1866 
   Size:        800    1066
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1347    1866 
   Size:       1347     519
 
BclI T'GATC_A

Cuts at:      0     648    1866 
   Size:        648    1218
 
BfrBI ATG'CAT

Cuts at:      0    1611    1866 
   Size:       1611     255
 
BglI GCCn_nnn'nGGC

Cuts at:      0     495    1866 
   Size:        495    1371
 
BglII A'GATC_T

Cuts at:      0     870    1479    1866 
   Size:        870     609     387

  Fragments arranged by size: 

                 870     609     387
 
Bme1580I G_kGCm'C

Cuts at:      0     513    1866 
   Size:        513    1353
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     462    1866 
   Size:        462    1404
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79    1213    1718    1808    1866 
   Size:         79    1134     505      90      58

  Fragments arranged by size: 

                1134     505      90      79      58
 
Bpu10I CC'TnA_GC

Cuts at:      0     800    1866 
   Size:        800    1066
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1468    1866 
   Size:       1468     398
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1107    1866 
   Size:       1107     759
 
BsaBI GATnn'nnATC

Cuts at:      0     943    1866 
   Size:        943     923
 
BsaWI w'CCGG_w

Cuts at:      0     756    1866 
   Size:        756    1110
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     788    1866 
   Size:        788    1078
 
BseYI C'CCAG_C

Cuts at:      0     962    1000    1197    1244    1866 
   Size:        962      38     197      47     622

  Fragments arranged by size: 

                 962     622     197      47      38
 
BsiEI CG_ry'CG

Cuts at:      0    1159    1866 
   Size:       1159     707
 
BsiHKAI G_wGCw'C

Cuts at:      0    1018    1866 
   Size:       1018     848
 
BsmI GAATG_Cn'

Cuts at:      0     174    1866 
   Size:        174    1692
 
Bsp1286I G_dGCh'C

Cuts at:      0     513    1018    1866 
   Size:        513     505     848

  Fragments arranged by size: 

                 848     513     505
 
BsrFI r'CCGG_y

Cuts at:      0     756    1866 
   Size:        756    1110
 
BsrGI T'GTAC_A

Cuts at:      0    1652    1866 
   Size:       1652     214
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1785    1866 
   Size:       1785      81
 
BstEII G'GTnAC_C

Cuts at:      0      86    1866 
   Size:         86    1780
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1636    1866 
   Size:       1636     230
 
BstYI r'GATC_y

Cuts at:      0     870    1479    1866 
   Size:        870     609     387

  Fragments arranged by size: 

                 870     609     387
 
BtsI GCAGTG_nn'

Cuts at:      0     363    1068    1419    1866 
   Size:        363     705     351     447

  Fragments arranged by size: 

                 705     447     363     351
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      20    1866 
   Size:         20    1846
 
EaeI y'GGCC_r

Cuts at:      0     496    1866 
   Size:        496    1370
 
EarI CTCTTCn'nnn_

Cuts at:      0     499    1866 
   Size:        499    1367
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79    1213    1667    1718    1808    1866 
   Size:         79    1134     454      51      90      58

  Fragments arranged by size: 

                1134     454      90      79      58      51
 
EcoO109I rG'GnC_Cy

Cuts at:      0     446    1866 
   Size:        446    1420
 
EcoRI G'AATT_C

Cuts at:      0     369    1866 
   Size:        369    1497
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      98     130     753     785    1866 
   Size:         98      32     623      32    1081

  Fragments arranged by size: 

                1081     623      98      32      32
 
HincII GTy'rAC

Cuts at:      0     928    1304    1866 
   Size:        928     376     562

  Fragments arranged by size: 

                 928     562     376
 
HpaI GTT'AAC

Cuts at:      0    1304    1866 
   Size:       1304     562
 
MfeI C'AATT_G

Cuts at:      0    1859    1866 
   Size:       1859       7
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     237     343     622     714    1073    1639    1866 
   Size:        237     106     279      92     359     566     227

  Fragments arranged by size: 

                 566     359     279     237     227     106      92
 
MslI CAynn'nnrTG

Cuts at:      0     224    1061    1866 
   Size:        224     837     805

  Fragments arranged by size: 

                 837     805     224
 
NsiI A_TGCA'T

Cuts at:      0    1613    1866 
   Size:       1613     253
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     708    1699    1866 
   Size:        708     991     167

  Fragments arranged by size: 

                 991     708     167
 
PfoI T'CCnGG_A

Cuts at:      0    1734    1835    1866 
   Size:       1734     101      31

  Fragments arranged by size: 

                1734     101      31
 
PpuMI rG'GwC_Cy

Cuts at:      0     446    1866 
   Size:        446    1420
 
PsiI TTA'TAA

Cuts at:      0     899    1866 
   Size:        899     967
 
SexAI A'CCwGG_T

Cuts at:      0    1502    1866 
   Size:       1502     364
 
SfcI C'TryA_G

Cuts at:      0     518    1866 
   Size:        518    1348
 
SgrAI Cr'CCGG_yG

Cuts at:      0     756    1866 
   Size:        756    1110
 
SmlI C'TyrA_G

Cuts at:      0    1483    1866 
   Size:       1483     383
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     297    1037    1142    1211    1223    1866 
   Size:        297     740     105      69      12     643

  Fragments arranged by size: 

                 740     643     297     105      69      12
 
TatI w'GTAC_w

Cuts at:      0     815    1006    1652    1866 
   Size:        815     191     646     214

  Fragments arranged by size: 

                 815     646     214     191
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1159    1866 
   Size:       1159     707
 

 Enzymes that do cut:

     AccI     AcuI     AgeI     ApoI     AseI     BanI    BbvCI    BciVI
     BclI    BfrBI     BglI    BglII Bme1580I     BmrI     BpmI   Bpu10I
    BpuEI     BsaI    BsaBI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BsrFI    BsrGI   BstAPI   BstEII    BstXI    BstYI
     BtsI     DrdI     EaeI     EarI  Eco57MI EcoO109I    EcoRI    Hin4I
   HincII     HpaI     MfeI     MmeI     MslI     NsiI    PflMI     PfoI
    PpuMI     PsiI    SexAI     SfcI    SgrAI     SmlI    TaqII     TatI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI     BcgI
     BlpI    BmgBI     BmtI     BplI    BsaAI    BsaHI    BsaXI     BsgI
    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI   BssHII
    BssSI    BstBI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
     DraI   DraIII     EagI     EciI  EcoICRI    EcoNI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII  HindIII     KasI     KpnI     MluI
     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NspI     PacI     PasI     PciI     PmeI     PmlI
     PpiI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
     SfiI     SfoI     SgfI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI
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