Restriction map for pF1KA0605
 (Linear) (Six-base) MAPSORT of: KIAA0605.seq  Check: 310  from: 1  to: 2853



KIAA0605.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:54  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1744    1918    2434    2853 
   Size:       1744     174     516     419

  Fragments arranged by size: 

                1744     516     419     174
 
AatII G_ACGT'C

Cuts at:      0    2633    2853 
   Size:       2633     220
 
Acc65I G'GTAC_C

Cuts at:      0     287    2853 
   Size:        287    2566
 
AclI AA'CG_TT

Cuts at:      0    1598    2853 
   Size:       1598    1255
 
AfeI AGC'GCT

Cuts at:      0    1047    2853 
   Size:       1047    1806
 
AflIII A'CryG_T

Cuts at:      0     368    1691    2282    2542    2853 
   Size:        368    1323     591     260     311

  Fragments arranged by size: 

                1323     591     368     311     260
 
AgeI A'CCGG_T

Cuts at:      0    1697    2853 
   Size:       1697    1156
 
AhdI GACnn_n'nnGTC

Cuts at:      0      72    2853 
   Size:         72    2781
 
AleI CACnn'nnGTG

Cuts at:      0     643    2363    2853 
   Size:        643    1720     490

  Fragments arranged by size: 

                1720     643     490
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1627    2853 
   Size:       1627    1226
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1884    1916    2853 
   Size:       1884      32     937

  Fragments arranged by size: 

                1884     937      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     266     927    1062    1386    1399    1808    2582    2853 
   Size:        266     661     135     324      13     409     774     271

  Fragments arranged by size: 

                 774     661     409     324     271     266     135      13
            
 
ApaI G_GGCC'C

Cuts at:      0     253     914    1189    1253    2060    2203    2853 
   Size:        253     661     275      64     807     143     650

  Fragments arranged by size: 

                 807     661     650     275     253     143      64
 
ApaLI G'TGCA_C

Cuts at:      0    2088    2592    2853 
   Size:       2088     504     261

  Fragments arranged by size: 

                2088     504     261
 
AvaI C'yCGr_G

Cuts at:      0     246     253     716    1164    1828    2020    2503    2530 
   Size:        246       7     463     448     664     192     483      27

Cuts at:   2530    2853 
   Size:        323

  Fragments arranged by size: 

                 664     483     463     448     323     246     192      27
                   7
 
BanI G'GyrC_C

Cuts at:      0     124     287     712    1453    2761    2853 
   Size:        124     163     425     741    1308      92

  Fragments arranged by size: 

                1308     741     425     163     124      92
 
BanII G_rGCy'C

Cuts at:      0     253     560     914    1189    1253    1468    1724    2060 
   Size:        253     307     354     275      64     215     256     336

Cuts at:   2060    2203    2516    2732    2853 
   Size:        143     313     216     121

  Fragments arranged by size: 

                 354     336     313     307     275     256     253     216
                 215     143     121      64
 
BbeI G_GCGC'C

Cuts at:      0    1457    2853 
   Size:       1457    1396
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     518     552    1546    1580    2074    2108    2529    2563 
   Size:        518      34     994      34     494      34     421      34

Cuts at:   2563    2853 
   Size:        290

  Fragments arranged by size: 

                 994     518     494     421     290      34      34      34
                  34
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1166    2853 
   Size:       1166    1687
 
BlpI GC'TnA_GC

Cuts at:      0    1549    2853 
   Size:       1549    1304
 
Bme1580I G_kGCm'C

Cuts at:      0     127     253     277     717     914    1189    1253    2060 
   Size:        127     126      24     440     197     275      64     807

Cuts at:   2060    2092    2203    2596    2775    2804    2853 
   Size:         32     111     393     179      29      49

  Fragments arranged by size: 

                 807     440     393     275     197     179     127     126
                 111      64      49      32      29      24
 
BmgBI CAC'GTC

Cuts at:      0     278     756    2853 
   Size:        278     478    2097

  Fragments arranged by size: 

                2097     478     278
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2055    2244    2319    2415    2853 
   Size:       2055     189      75      96     438

  Fragments arranged by size: 

                2055     438     189      96      75
 
BmtI G_CTAG'C

Cuts at:      0     763    2853 
   Size:        763    2090
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1374    2853 
   Size:       1374    1479
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     136     414    1564    1911    2853 
   Size:        136     278    1150     347     942

  Fragments arranged by size: 

                1150     942     347     278     136
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     874    1198    1268    2332    2853 
   Size:        874     324      70    1064     521

  Fragments arranged by size: 

                1064     874     521     324      70
 
BsaAI yAC'GTr

Cuts at:      0     369     645    2283    2543    2853 
   Size:        369     276    1638     260     310

  Fragments arranged by size: 

                1638     369     310     276     260
 
BsaHI Gr'CG_yC

Cuts at:      0     131    1454    1787    1814    2630    2853 
   Size:        131    1323     333      27     816     223

  Fragments arranged by size: 

                1323     816     333     223     131      27
 
BsaWI w'CCGG_w

Cuts at:      0     883    1697    2853 
   Size:        883     814    1156

  Fragments arranged by size: 

                1156     883     814
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1087    1117    1278    1308    1327    1357    1561    1591 
   Size:       1087      30     161      30      19      30     204      30

Cuts at:   1591    1884    1914    2527    2557    2853 
   Size:        293      30     613      30     296

  Fragments arranged by size: 

                1087     613     296     293     204     161      30      30
                  30      30      30      30      19
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     246     342     355    1113    1286    1513    1566    2548 
   Size:        246      96      13     758     173     227      53     982

Cuts at:   2548    2853 
   Size:        305

  Fragments arranged by size: 

                 982     758     305     246     227     173      96      53
                  13
 
BseYI C'CCAG_C

Cuts at:      0      26      61    1016    1134    1182    1189    1386    1852 
   Size:         26      35     955     118      48       7     197     466

Cuts at:   1852    2853 
   Size:       1001

  Fragments arranged by size: 

                1001     955     466     197     118      48      35      26
                   7
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     252     325     433     621    1722    2178    2265    2853 
   Size:        252      73     108     188    1101     456      87     588

  Fragments arranged by size: 

                1101     588     456     252     188     108      87      73
            
 
BsiEI CG_ry'CG

Cuts at:      0    1625    2853 
   Size:       1625    1228
 
BsiHKAI G_wGCw'C

Cuts at:      0    1468    2092    2459    2542    2596    2853 
   Size:       1468     624     367      83      54     257

  Fragments arranged by size: 

                1468     624     367     257      83      54
 
BsiWI C'GTAC_G

Cuts at:      0    1760    2853 
   Size:       1760    1093
 
BsmI GAATG_Cn'

Cuts at:      0     675    2853 
   Size:        675    2178
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     357    1873    2232    2621    2853 
   Size:        357    1516     359     389     232

  Fragments arranged by size: 

                1516     389     359     357     232
 
Bsp1286I G_dGCh'C

Cuts at:      0     127     253     277     560     717     914    1189    1253 
   Size:        127     126      24     283     157     197     275      64

Cuts at:   1253    1468    1724    2060    2092    2203    2459    2516    2542 
   Size:        215     256     336      32     111     256      57      26

Cuts at:   2542    2596    2732    2775    2804    2853 
   Size:         54     136      43      29      49

  Fragments arranged by size: 

                 336     283     275     256     256     215     197     157
                 136     127     126     111      64      57      54      49
                  43      32      29      26      24
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     274     455     533    1532    1744    1918    2044    2434 
   Size:        274     181      78     999     212     174     126     390

Cuts at:   2434    2611    2853 
   Size:        177     242

  Fragments arranged by size: 

                 999     390     274     242     212     181     177     174
                 126      78
 
BsrBI CCG'CTC

Cuts at:      0     213    1131    2412    2831    2853 
   Size:        213     918    1281     419      22

  Fragments arranged by size: 

                1281     918     419     213      22
 
BsrDI GCAATG_nn'

Cuts at:      0      22    2853 
   Size:         22    2831
 
BsrFI r'CCGG_y

Cuts at:      0     857    1148    1228    1697    2213    2853 
   Size:        857     291      80     469     516     640

  Fragments arranged by size: 

                 857     640     516     469     291      80
 
BsrGI T'GTAC_A

Cuts at:      0     371    1982    2853 
   Size:        371    1611     871

  Fragments arranged by size: 

                1611     871     371
 
BssHII G'CGCG_C

Cuts at:      0    1663    2853 
   Size:       1663    1190
 
BssSI C'ACGA_G

Cuts at:      0    1003    1717    1840    2853 
   Size:       1003     714     123    1013

  Fragments arranged by size: 

                1013    1003     714     123
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     222     509    2036    2822    2853 
   Size:        222     287    1527     786      31

  Fragments arranged by size: 

                1527     786     287     222      31
 
BstEII G'GTnAC_C

Cuts at:      0     693    2118    2853 
   Size:        693    1425     735

  Fragments arranged by size: 

                1425     735     693
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     114    2349    2365    2853 
   Size:        114    2235      16     488

  Fragments arranged by size: 

                2235     488     114      16
 
BtgI C'CryG_G

Cuts at:      0      86     134     461     866    1685    2661    2723    2853 
   Size:         86      48     327     405     819     976      62     130

  Fragments arranged by size: 

                 976     819     405     327     130      86      62      48
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     819    2853 
   Size:        819    2034
 
BtsI GCAGTG_nn'

Cuts at:      0     216     352    1734    2090    2118    2224    2253    2502 
   Size:        216     136    1382     356      28     106      29     249

Cuts at:   2502    2853 
   Size:        351

  Fragments arranged by size: 

                1382     356     351     249     216     136     106      29
                  28
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1282    2853 
   Size:       1282    1571
 
EaeI y'GGCC_r

Cuts at:      0     468    1113    1158    1472    1622    2351    2471    2777 
   Size:        468     645      45     314     150     729     120     306

Cuts at:   2777    2853 
   Size:         76

  Fragments arranged by size: 

                 729     645     468     314     306     150     120      76
                  45
 
EagI C'GGCC_G

Cuts at:      0    1622    2853 
   Size:       1622    1231
 
EarI CTCTTCn'nnn_

Cuts at:      0    1316    2853 
   Size:       1316    1537
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     305     396    2853 
   Size:        305      91    2457

  Fragments arranged by size: 

                2457     305      91
 
EcoICRI GAG'CTC

Cuts at:      0    1466    2853 
   Size:       1466    1387
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     136     414    1564    1911    2853 
   Size:        136     278    1150     347     942

  Fragments arranged by size: 

                1150     942     347     278     136
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1996    2853 
   Size:       1996     857
 
EcoO109I rG'GnC_Cy

Cuts at:      0     250     263     910     924    1059    1186    1238    1249 
   Size:        250      13     647      14     135     127      52      11

Cuts at:   1249    1250    1535    1584    2057    2555    2840    2853 
   Size:          1     285      49     473     498     285      13

  Fragments arranged by size: 

                 647     498     473     285     285     250     135     127
                  52      49      14      13      13      11       1
 
HaeII r_GCGC'y

Cuts at:      0    1049    1457    2853 
   Size:       1049     408    1396

  Fragments arranged by size: 

                1396    1049     408
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      58      90     490     522    2331    2363    2853 
   Size:         58      32     400      32    1809      32     490

  Fragments arranged by size: 

                1809     490     400      58      32      32      32
 
HincII GTy'rAC

Cuts at:      0      77    1329    2853 
   Size:         77    1252    1524

  Fragments arranged by size: 

                1524    1252      77
 
KasI G'GCGC_C

Cuts at:      0    1453    2853 
   Size:       1453    1400
 
KpnI G_GTAC'C

Cuts at:      0     291    2853 
   Size:        291    2562
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2054    2255    2853 
   Size:       2054     201     598

  Fragments arranged by size: 

                2054     598     201
 
MscI TGG'CCA

Cuts at:      0    2353    2473    2779    2853 
   Size:       2353     120     306      74

  Fragments arranged by size: 

                2353     306     120      74
 
MslI CAynn'nnrTG

Cuts at:      0     643    2363    2785    2853 
   Size:        643    1720     422      68

  Fragments arranged by size: 

                1720     643     422      68
 
MspA1I CmG'CkG

Cuts at:      0     474     635     751    1383    1687    1889    2135    2744 
   Size:        474     161     116     632     304     202     246     609

Cuts at:   2744    2853 
   Size:        109

  Fragments arranged by size: 

                 632     609     474     304     246     202     161     116
                 109
 
NaeI GCC'GGC

Cuts at:      0    1230    2853 
   Size:       1230    1623
 
NarI GG'CG_CC

Cuts at:      0    1454    2853 
   Size:       1454    1399
 
NcoI C'CATG_G

Cuts at:      0     866    2853 
   Size:        866    1987
 
NgoMIV G'CCGG_C

Cuts at:      0    1228    2853 
   Size:       1228    1625
 
NheI G'CTAG_C

Cuts at:      0     759    2853 
   Size:        759    2094
 
NspI r_CATG'y

Cuts at:      0    1695    2853 
   Size:       1695    1158
 
PasI CC'CwG_GG

Cuts at:      0    1256    2853 
   Size:       1256    1597
 
PciI A'CATG_T

Cuts at:      0    1691    2853 
   Size:       1691    1162
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     597    1062    1386    2394    2582    2853 
   Size:        597     465     324    1008     188     271

  Fragments arranged by size: 

                1008     597     465     324     271     188
 
PmlI CAC'GTG

Cuts at:      0     369     645    2283    2543    2853 
   Size:        369     276    1638     260     310

  Fragments arranged by size: 

                1638     369     310     276     260
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1884    1916    2853 
   Size:       1884      32     937

  Fragments arranged by size: 

                1884     937      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     263    1535    2853 
   Size:        263    1272    1318

  Fragments arranged by size: 

                1318    1272     263
 
PspOMI G'GGCC_C

Cuts at:      0     249     910    1185    1249    2056    2199    2853 
   Size:        249     661     275      64     807     143     654

  Fragments arranged by size: 

                 807     661     654     275     249     143      64
 
PstI C_TGCA'G

Cuts at:      0     227     301     995    1729    2714    2839    2853 
   Size:        227      74     694     734     985     125      14

  Fragments arranged by size: 

                 985     734     694     227     125      74      14
 
PvuII CAG'CTG

Cuts at:      0     635    1383    1889    2744    2853 
   Size:        635     748     506     855     109

  Fragments arranged by size: 

                 855     748     635     506     109
 
SacI G_AGCT'C

Cuts at:      0    1468    2853 
   Size:       1468    1385
 
SacII CC_GC'GG

Cuts at:      0    1688    2853 
   Size:       1688    1165
 
SbfI CC_TGCA'GG

Cuts at:      0    2839    2853 
   Size:       2839      14
 
SfcI C'TryA_G

Cuts at:      0     223     297     991    1725    2177    2710    2811    2835 
   Size:        223      74     694     734     452     533     101      24

Cuts at:   2835    2853 
   Size:         18

  Fragments arranged by size: 

                 734     694     533     452     223     101      74      24
                  18
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1166    2853 
   Size:       1166    1687
 
SfoI GGC'GCC

Cuts at:      0    1455    2853 
   Size:       1455    1398
 
SmaI CCC'GGG

Cuts at:      0     248     255    1166    2853 
   Size:        248       7     911    1687

  Fragments arranged by size: 

                1687     911     248       7
 
StuI AGG'CCT

Cuts at:      0    1120    2175    2701    2853 
   Size:       1120    1055     526     152

  Fragments arranged by size: 

                1120    1055     526     152
 
StyI C'CwwG_G

Cuts at:      0     678     866    1459    1658    2853 
   Size:        678     188     593     199    1195

  Fragments arranged by size: 

                1195     678     593     199     188
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     604     655    1348    2665    2853 
   Size:        604      51     693    1317     188

  Fragments arranged by size: 

                1317     693     604     188      51
 
TatI w'GTAC_w

Cuts at:      0     371     893     980    1982    2285    2853 
   Size:        371     522      87    1002     303     568

  Fragments arranged by size: 

                1002     568     522     371     303      87
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1087    1119    1278    1310    1327    1359    2527    2559 
   Size:       1087      32     159      32      17      32    1168      32

Cuts at:   2559    2853 
   Size:        294

  Fragments arranged by size: 

                1168    1087     294     159      32      32      32      32
                  17
 
Tth111I GACn'n_nGTC

Cuts at:      0     418    2656    2853 
   Size:        418    2238     197

  Fragments arranged by size: 

                2238     418     197
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     594     706    2772    2853 
   Size:        594     112    2066      81

  Fragments arranged by size: 

                2066     594     112      81
 
XmaI C'CCGG_G

Cuts at:      0     246     253    1164    2853 
   Size:        246       7     911    1689

  Fragments arranged by size: 

                1689     911     246       7
 
XmnI GAAnn'nnTTC

Cuts at:      0    1411    2853 
   Size:       1411    1442
 
ZraI GAC'GTC

Cuts at:      0    2631    2853 
   Size:       2631     222
 

 Enzymes that do cut:

     AarI    AatII   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AvaI     BanI
    BanII     BbeI     BcgI     BglI     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI   BstEII    BstXI     BtgI    BtgZI     BtsI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII
    Hin4I   HincII     KasI     KpnI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NheI     NspI     PasI     PciI
    PflMI     PmlI     PpiI    PpuMI   PspOMI     PstI    PvuII     SacI
    SacII     SbfI     SfcI     SfiI     SfoI     SmaI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AccI     AcuI    AflII     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbsI    BbvCI    BciVI     BclI    BfrBI    BglII
   Bpu10I    BpuEI    BsaBI    BspEI    BspHI    BstBI    BstYI  BstZ17I
   Bsu36I     ClaI    CspCI     DraI   DraIII    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI  HindIII     HpaI     MfeI     MluI     NdeI
     NotI     NruI     NsiI     PacI     PfoI     PmeI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII     SalI    SanDI     SapI     ScaI
    SexAI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     XbaI     XhoI
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