Restriction map for pF1KA0600
##################################
# NO CUT ENZYMES (   93 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        AhdI        Alw44I      Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        BamHI       BbrPI       BclI        
BfrI        BglII       BsaAI       BsaBI       BsaWI       
BseAI       BsiEI       BsiWI       BsmI        Bsp1407I    
BspDI       BspEI       BspLU11I    BsrGI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
ClaI        CpoI        DraI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO65I     EcoRV       
EcoT22I     FbaI        FseI        HindIII     HpaI        
KpnI        MamI        MfeI        MluI        MroI        
MunI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        RsrII       SbfI        ScaI        
SexAI       SfuI        SgrAI       SnaBI       Sse8387I    
SspBI       SspI        StuI        SwaI        Tth111I     
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccI         1179  GTCGAC               GT^[AC][GT]AC
AcsI          565  GAATTC               [GA]^AATT[CT]
AocI         1645  CCTGAGG              CC^T[ACGT]AGG
ApoI          565  GAATTC               [GA]^AATT[CT]
AviII        2147  TGCGCA               TGC^GCA
AvrII        1119  CCTAGG               C^CTAGG
BbeI         1929  GGCGCC               GGCGC^C
BcgI         1827  CGAGAGTGGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1873  GGATAC               GTATCC(6/5)
BlnI         1119  CCTAGG               C^CTAGG
BlpI          820  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      820  GCTGAGC              GC^T[ACGT]AGC
BsaI         2980  GAGACC               GGTCTC(1/5)
BspHI        1250  TCATGA               T^CATGA
BssSI        2593  CACGAG               CACGAG(-5/-1)
Bsu36I       1645  CCTGAGG              CC^T[ACGT]AGG
CelII         820  GCTGAGC              GC^T[ACGT]AGC
DraIII       2112  CACCGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         2020  GACCAGCCCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1645  CCTGAGG              CC^T[ACGT]AGG
EcoRI         565  GAATTC               G^AATTC
FspI         2147  TGCGCA               TGC^GCA
HincII       1179  GTCGAC               GT[CT]^[GA]AC
HindII       1179  GTCGAC               GT[CT]^[GA]AC
KasI         1929  GGCGCC               G^GCGCC
NaeI         3075  GCCGGC               GCC^GGC
NarI         1929  GGCGCC               GG^CGCC
NgoMIV       3075  GCCGGC               G^CCGGC
PpuMI        2333  AGGTCCT              [GA]G^G[AT]CC[CT]
PshAI        2648  GACATCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         1250  TCATGA               T^CATGA
SalI         1179  GTCGAC               G^TCGAC
SfiI         3030  GGCCGAGCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         1929  GGCGCC               GGC^GCC
SpeI         1410  ACTAGT               A^CTAGT
Tsp509I       566  AATT                 ^AATT
XcmI         2537  CCATTGGAGACGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccII          62  CGCG                 CG^CG
             1463  CGCG                
AflIII       1091  ACACGT               A^C[GA][CT]GT
             1425  ACGTGT              
AlwI         1599  GATCC                GGATC(4/5)
             2901  GATCC               
ApaI          730  GGGCCC               GGGCC^C
             1846  GGGCCC              
AvaI         1715  CCCGGG               C^[CT]CG[GA]G
             3056  CCCGGG              
BbsI         1750  GAAGAC               GAAGAC(2/6)
             1774  GAAGAC              
BpuAI        1750  GAAGAC               GAAGAC(2/6)
             1774  GAAGAC              
BsgI           73  CTGCAC               GTGCAG(16/14)
             2926  GTGCAG              
BsoBI        1715  CCCGGG               C^[CT]CG[GA]G
             3056  CCCGGG              
BspMI        1917  GCAGGT               ACCTGC(4/8)
             2974  GCAGGT              
BsrBI         684  GAGCGG               CCGCTC(-3/-3)
              823  GAGCGG              
BstUI          62  CGCG                 CG^CG
             1463  CGCG                
BstYI        1598  AGATCC               [GA]^GATC[CT]
             2900  AGATCC              
KspI           61  CCGCGG               CCGC^GG
             1462  CCGCGG              
MflI         1598  AGATCC               [GA]^GATC[CT]
             2900  AGATCC              
MseI          878  TTAA                 T^TAA
             1811  TTAA                
MvnI           62  CGCG                 CG^CG
             1463  CGCG                
PleI           16  GAGTC                GAGTC(4/5)
             1509  GAGTC               
SacI          235  GAGCTC               GAGCT^C
             2200  GAGCTC              
SacII          61  CCGCGG               CCGC^GG
             1462  CCGCGG              
SmaI         1715  CCCGGG               CCC^GGG
             3056  CCCGGG              
SphI         1320  GCATGC               GCATG^C
             1439  GCATGC              
TfiI         2251  GAATC                G^A[AT]TC
             2267  GATTC               
Tru9I         878  TTAA                 T^TAA
             1811  TTAA                
XhoII        1598  AGATCC               [GA]^GATC[CT]
             2900  AGATCC              
XmaCI        1715  CCCGGG               C^CCGGG
             3056  CCCGGG              
XmaI         1715  CCCGGG               C^CCGGG
             3056  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  101 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwNI       
AspHI       AvaII       BalI        BanI        BanII       
BbvCI       BbvI        BcnI        BfaI        BglI        
BmyI        BpmI        BsaHI       BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       Bsp1286I    BsrDI       BsrFI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstXI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MluNI       
MnlI        MscI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       NspI        PflMI       PstI        
PvuII       RsaI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        TseI        Tsp45I      TspRI       TthHB8I     
Van91I      
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