Restriction map for
pF1KA0596
(Circular) (Six-base) MAPSORT of: pF1KA0596.seq Check: 4171 from: 1 to: 7655
pF1KA0596 7655 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 1298 2972 4693 1298
Size: 1674 1721 4260
Fragments arranged by size:
4260 1721 1674
Acc65I G'GTAC_C
Cuts at: 4671 4671
Size: 7655
AclI AA'CG_TT
Cuts at: 7495 7495
Size: 7655
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1615 1672 2516 3173 4478 5480 5912 6265 6927
Size: 57 844 657 1305 1002 432 353 662
Cuts at: 6927 1615
Size: 2343
Fragments arranged by size:
2343 1305 1002 844 662 657 432 353
57
AflIII A'CryG_T
Cuts at: 590 5007 6807 7088 590
Size: 4417 1800 281 1157
Fragments arranged by size:
4417 1800 1157 281
AleI CACnn'nnGTG
Cuts at: 814 814
Size: 7655
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 464 464
Size: 7655
AlwNI CAG_nnn'CTG
Cuts at: 256 1182 1485 1638 1794 2337 2641 3101 3125
Size: 926 303 153 156 543 304 460 24
Cuts at: 3125 3601 3641 4211 4224 4509 4587 4960 6398
Size: 476 40 570 13 285 78 373 1438
Cuts at: 6398 256
Size: 1513
Fragments arranged by size:
1513 1438 926 570 543 476 460 373
304 303 285 156 153 78 40 24
13
ApaI G_GGCC'C
Cuts at: 3262 3360 4071 4202 3262
Size: 98 711 131 6715
Fragments arranged by size:
6715 711 131 98
ApaLI G'TGCA_C
Cuts at: 1621 3703 6493 1621
Size: 2082 2790 2783
Fragments arranged by size:
2790 2783 2082
ApoI r'AATT_y
Cuts at: 709 2236 4659 7008 7264 709
Size: 1527 2423 2349 256 1100
Fragments arranged by size:
2423 2349 1527 1100 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7655
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7655
AvaI C'yCGr_G
Cuts at: 1303 3035 3773 4675 1303
Size: 1732 738 902 4283
Fragments arranged by size:
4283 1732 902 738
AvrII C'CTAG_G
Cuts at: 4107 4171 4107
Size: 64 7591
BamHI G'GATC_C
Cuts at: 1536 4680 1536
Size: 3144 4511
BanI G'GyrC_C
Cuts at: 1006 2297 2417 3492 3529 3714 4156 4671 5085
Size: 1291 120 1075 37 185 442 515 414
Cuts at: 5085 5333 5368 1006
Size: 248 35 3293
Fragments arranged by size:
3293 1291 1075 515 442 414 248 185
120 37 35
BanII G_rGCy'C
Cuts at: 1399 1985 2308 3076 3176 3262 3360 3648 3805
Size: 586 323 768 100 86 98 288 157
Cuts at: 3805 3990 4071 4202 4385 4669 5699 1399
Size: 185 81 131 183 284 1030 3355
Fragments arranged by size:
3355 1030 768 586 323 288 284 185
183 157 131 100 98 86 81
BbeI G_GCGC'C
Cuts at: 2301 5089 5337 2301
Size: 2788 248 4619
Fragments arranged by size:
4619 2788 248
BbsI GAAGACnn'nnnn_
Cuts at: 1960 3161 4288 1960
Size: 1201 1127 5327
Fragments arranged by size:
5327 1201 1127
BbvCI CC'TCA_GC
Cuts at: 211 922 1435 3218 4446 211
Size: 711 513 1783 1228 3420
Fragments arranged by size:
3420 1783 1228 711 513
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2534 2568 3458 3492 2534
Size: 34 890 34 6697
Fragments arranged by size:
6697 890 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1148 2997 5553 6609 7655 1148
Size: 1849 2556 1056 1046 1148
Fragments arranged by size:
2556 1849 1148 1056 1046
BclI T'GATC_A
Cuts at: 1859 5175 1859
Size: 3316 4339
BfrBI ATG'CAT
Cuts at: 1525 1525
Size: 7655
BglI GCCn_nnn'nGGC
Cuts at: 2859 2859
Size: 7655
BglII A'GATC_T
Cuts at: 157 2937 5170 7207 157
Size: 2780 2233 2037 605
Fragments arranged by size:
2780 2233 2037 605
BlpI GC'TnA_GC
Cuts at: 4767 4767
Size: 7655
Bme1580I G_kGCm'C
Cuts at: 443 761 1612 1625 2610 3262 3360 3707 4071
Size: 318 851 13 985 652 98 347 364
Cuts at: 4071 4159 4202 4218 5280 5373 6497 443
Size: 88 43 16 1062 93 1124 1601
Fragments arranged by size:
1601 1124 1062 985 851 652 364 347
318 98 93 88 43 16 13
BmgBI CAC'GTC
Cuts at: 468 863 468
Size: 395 7260
BmrI ACTGGGnnnn_n'
Cuts at: 1334 2601 3355 3690 4867 4948 5283 7427 1334
Size: 1267 754 335 1177 81 335 2144 1562
Fragments arranged by size:
2144 1562 1267 1177 754 335 335 81
BmtI G_CTAG'C
Cuts at: 1091 1726 1091
Size: 635 7020
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 226 4617 6022 226
Size: 4391 1405 1859
Fragments arranged by size:
4391 1859 1405
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 278 449 1139 1305 1526 1708 1931 3014 3278
Size: 171 690 166 221 182 223 1083 264
Cuts at: 3278 3335 3563 3670 4375 4612 278
Size: 57 228 107 705 237 3321
Fragments arranged by size:
3321 1083 705 690 264 237 228 223
221 182 171 166 107 57
Bpu10I CC'TnA_GC
Cuts at: 211 774 922 1045 1435 2302 3218 3788 4324
Size: 563 148 123 390 867 916 570 536
Cuts at: 4324 4446 211
Size: 122 3420
Fragments arranged by size:
3420 916 867 570 563 536 390 148
123 122
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2842 3444 3914 4831 5710 6424 6722 2842
Size: 602 470 917 879 714 298 3775
Fragments arranged by size:
3775 917 879 714 602 470 298
BsaI GGTCTCn'nnnn_
Cuts at: 3474 7359 3474
Size: 3885 3770
BsaAI yAC'GTr
Cuts at: 1944 5008 1944
Size: 3064 4591
BsaBI GATnn'nnATC
Cuts at: 6 162 2317 5169 7206 6
Size: 156 2155 2852 2037 455
Fragments arranged by size:
2852 2155 2037 455 156
BsaHI Gr'CG_yC
Cuts at: 2298 5086 5334 6036 7532 2298
Size: 2788 248 702 1496 2421
Fragments arranged by size:
2788 2421 1496 702 248
BsaWI w'CCGG_w
Cuts at: 4844 5365 6043 6454 6601 6875 4844
Size: 521 678 411 147 274 5624
Fragments arranged by size:
5624 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 199 229 493 523 1795 1825 1904 1934 199
Size: 30 264 30 1272 30 79 30 5920
Fragments arranged by size:
5920 1272 264 79 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1350 1523 2169 3186 3259 3447 4465 1350
Size: 173 646 1017 73 188 1018 4540
Fragments arranged by size:
4540 1018 1017 646 188 173 73
BseYI C'CCAG_C
Cuts at: 240 306 765 783 1984 2560 2565 2634 2675
Size: 66 459 18 1201 576 5 69 41
Cuts at: 2675 3580 3587 3641 3813 3979 4201 4217 4469
Size: 905 7 54 172 166 222 16 252
Cuts at: 4469 4522 5080 5838 6503 6911 240
Size: 53 558 758 665 408 984
Fragments arranged by size:
1201 984 905 758 665 576 558 459
408 252 222 172 166 69 66 54
53 41 18 16 7 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1021 1942 2128 2933 3043 3385 1021
Size: 921 186 805 110 342 5291
Fragments arranged by size:
5291 921 805 342 186 110
BsiEI CG_ry'CG
Cuts at: 104 5197 5243 6473 104
Size: 5093 46 1230 1286
Fragments arranged by size:
5093 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 479 506 1399 1625 2308 3076 3146 3169 3549
Size: 27 893 226 683 768 70 23 380
Cuts at: 3549 3707 4385 4669 5447 5637 6497 479
Size: 158 678 284 778 190 860 1637
Fragments arranged by size:
1637 893 860 778 768 683 678 380
284 226 190 158 70 27 23
BsmI GAATG_Cn'
Cuts at: 753 1134 4042 4306 753
Size: 381 2908 264 4102
Fragments arranged by size:
4102 2908 381 264
BsmBI CGTCTCn'nnnn_
Cuts at: 3628 3836 3628
Size: 208 7447
Bsp1286I G_dGCh'C
Cuts at: 443 479 506 761 1399 1612 1625 1985 2308
Size: 36 27 255 638 213 13 360 323
Cuts at: 2308 2610 3076 3146 3169 3176 3262 3360 3549
Size: 302 466 70 23 7 86 98 189
Cuts at: 3549 3648 3707 3805 3990 4071 4159 4202 4218
Size: 99 59 98 185 81 88 43 16
Cuts at: 4218 4385 4669 5280 5373 5447 5637 5699 6497
Size: 167 284 611 93 74 190 62 798
Cuts at: 6497 443
Size: 1601
Fragments arranged by size:
1601 798 638 611 466 360 323 302
284 255 213 190 189 185 167 99
98 98 93 88 86 81 74 70
62 59 43 36 27 23 16 13
7
BspHI T'CATG_A
Cuts at: 6087 6087
Size: 7655
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1043 1363 2955 3357 4705 4919 5221 5602 1043
Size: 320 1592 402 1348 214 302 381 3096
Fragments arranged by size:
3096 1592 1348 402 381 320 302 214
BsrBI CCG'CTC
Cuts at: 50 773 1556 5947 6001 7485 7650 50
Size: 723 783 4391 54 1484 165 55
Fragments arranged by size:
4391 1484 783 723 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1082 1148 2132 3531 5567 1082
Size: 66 984 1399 2036 3170
Fragments arranged by size:
3170 2036 1399 984 66
BsrFI r'CCGG_y
Cuts at: 1742 2414 2448 5653 5834 1742
Size: 672 34 3205 181 3563
Fragments arranged by size:
3563 3205 672 181 34
BsrGI T'GTAC_A
Cuts at: 1193 1193
Size: 7655
BssSI C'ACGA_G
Cuts at: 5926 6634 5926
Size: 708 6947
BstAPI GCAn_nnn'nTGC
Cuts at: 3125 5032 3125
Size: 1907 5748
BstBI TT'CG_AA
Cuts at: 6016 6016
Size: 7655
BstEII G'GTnAC_C
Cuts at: 222 222
Size: 7655
BstXI CCAn_nnnn'nTGG
Cuts at: 114 1242 3657 3870 4296 114
Size: 1128 2415 213 426 3473
Fragments arranged by size:
3473 2415 1128 426 213
BstYI r'GATC_y
Cuts at: 1 157 1536 2126 2707 2718 2937 4680 5147
Size: 156 1379 590 581 11 219 1743 467
Cuts at: 5147 5170 5505 5751 6057 6069 6155 7207 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1743 1379 1052 590 581 467 449 335
306 246 219 156 86 23 12 11
Bsu36I CC'TnA_GG
Cuts at: 867 2121 2181 2864 867
Size: 1254 60 683 5658
Fragments arranged by size:
5658 1254 683 60
BtgI C'CryG_G
Cuts at: 107 2618 2999 3869 4295 4386 4622 107
Size: 2511 381 870 426 91 236 3140
Fragments arranged by size:
3140 2511 870 426 381 236 91
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5605 5786 5949 5605
Size: 181 163 7311
Fragments arranged by size:
7311 181 163
BtsI GCAGTG_nn'
Cuts at: 386 659 686 957 1446 1817 1895 2052 2512
Size: 273 27 271 489 371 78 157 460
Cuts at: 2512 3763 4015 4366 4412 4535 4898 386
Size: 1251 252 351 46 123 363 3143
Fragments arranged by size:
3143 1251 489 460 371 363 351 273
271 252 157 123 78 46 27
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 932 967 2187 2222 932
Size: 35 1220 35 6365
Fragments arranged by size:
6365 1220 35 35
DraI TTT'AAA
Cuts at: 2070 4654 2070
Size: 2584 5071
DraIII CAC_nnn'GTG
Cuts at: 1944 1947 2083 4425 1944
Size: 3 136 2342 5174
Fragments arranged by size:
5174 2342 136 3
DrdI GACnn_nn'nnGTC
Cuts at: 583 654 5361 6705 583
Size: 71 4707 1344 1533
Fragments arranged by size:
4707 1533 1344 71
EaeI y'GGCC_r
Cuts at: 488 2195 2208 2339 2777 3335 3659 3872 5240
Size: 1707 13 131 438 558 324 213 1368
Cuts at: 5240 5414 5805 5832 7177 488
Size: 174 391 27 1345 966
Fragments arranged by size:
1707 1368 1345 966 558 438 391 324
213 174 131 27 13
EagI C'GGCC_G
Cuts at: 5240 5240
Size: 7655
EarI CTCTTCn'nnn_
Cuts at: 1117 2661 3045 3203 3227 3236 3405 4246 4497
Size: 1544 384 158 24 9 169 841 251
Cuts at: 4497 5678 5888 1117
Size: 1181 210 2884
Fragments arranged by size:
2884 1544 1181 841 384 251 210 169
158 24 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 856 1366 5863 6605 6751 7465 856
Size: 510 4497 742 146 714 1046
Fragments arranged by size:
4497 1046 742 714 510 146
EcoICRI GAG'CTC
Cuts at: 1397 2306 3074 4383 4667 1397
Size: 909 768 1309 284 4385
Fragments arranged by size:
4385 1309 909 768 284
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 278 449 1139 1305 1526 1615 1672 1708 1931
Size: 171 690 166 221 89 57 36 223
Cuts at: 1931 2516 3014 3173 3278 3335 3563 3670 4375
Size: 585 498 159 105 57 228 107 705
Cuts at: 4375 4478 4612 5480 5912 6265 6927 278
Size: 103 134 868 432 353 662 1006
Fragments arranged by size:
1006 868 705 690 662 585 498 432
353 228 223 221 171 166 159 134
107 105 103 89 57 57 36
EcoNI CCTnn'n_nnAGG
Cuts at: 3755 4327 3755
Size: 572 7083
EcoO109I rG'GnC_Cy
Cuts at: 207 1230 1482 2424 2656 2757 2817 3259 3356
Size: 1023 252 942 232 101 60 442 97
Cuts at: 3356 3357 3369 3747 4068 4249 4584 4794 207
Size: 1 12 378 321 181 335 210 3068
Fragments arranged by size:
3068 1023 942 442 378 335 321 252
232 210 181 101 97 60 12 1
EcoRI G'AATT_C
Cuts at: 4659 4659
Size: 7655
EcoRV GAT'ATC
Cuts at: 3881 3881
Size: 7655
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 243 2521 4110 5674 243
Size: 2278 1589 1564 2224
Fragments arranged by size:
2278 2224 1589 1564
FspI TGC'GCA
Cuts at: 178 1901 5436 178
Size: 1723 3535 2397
Fragments arranged by size:
3535 2397 1723
HaeII r_GCGC'y
Cuts at: 545 2301 2676 5089 5337 6567 7338 545
Size: 1756 375 2413 248 1230 771 862
Fragments arranged by size:
2413 1756 1230 862 771 375 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 132 164 199 231 271 303 1149 1181 1789
Size: 32 35 32 40 32 846 32 608
Cuts at: 1789 1821 2164 2196 2319 2351 2438 2470 3178
Size: 32 343 32 123 32 87 32 708
Cuts at: 3178 3210 3608 3640 3771 3803 4474 4506 5497
Size: 32 398 32 131 32 671 32 991
Cuts at: 5497 5527 5529 5559 132
Size: 30 2 30 2228
Fragments arranged by size:
2228 991 846 708 671 608 398 343
131 123 87 40 35 32 32 32
32 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 125 588 4694 125
Size: 463 4106 3086
Fragments arranged by size:
4106 3086 463
KasI G'GCGC_C
Cuts at: 2297 5085 5333 2297
Size: 2788 248 4619
Fragments arranged by size:
4619 2788 248
KpnI G_GTAC'C
Cuts at: 4675 4675
Size: 7655
MluI A'CGCG_T
Cuts at: 7088 7088
Size: 7655
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 650 1514 4441 6414 6598 650
Size: 864 2927 1973 184 1707
Fragments arranged by size:
2927 1973 1707 864 184
MscI TGG'CCA
Cuts at: 2197 2210 2779 3337 3661 3874 5416 2197
Size: 13 569 558 324 213 1542 4436
Fragments arranged by size:
4436 1542 569 558 324 213 13
MslI CAynn'nnrTG
Cuts at: 112 509 616 814 967 1462 1975 2104 2230
Size: 397 107 198 153 495 513 129 126
Cuts at: 2230 2266 2290 2323 5644 5771 112
Size: 36 24 33 3321 127 1996
Fragments arranged by size:
3321 1996 513 495 397 198 153 129
127 126 107 36 33 24
MspA1I CmG'CkG
Cuts at: 1865 1988 2144 2282 2361 2730 3125 3217 3641
Size: 123 156 138 79 369 395 92 424
Cuts at: 3641 3817 4345 4371 4388 4766 5080 5440 6224
Size: 176 528 26 17 378 314 360 784
Cuts at: 6224 6469 1865
Size: 245 3051
Fragments arranged by size:
3051 784 528 424 395 378 369 360
314 245 176 156 138 123 92 79
26 17
NaeI GCC'GGC
Cuts at: 5836 5836
Size: 7655
NarI GG'CG_CC
Cuts at: 2298 5086 5334 2298
Size: 2788 248 4619
Fragments arranged by size:
4619 2788 248
NcoI C'CATG_G
Cuts at: 107 2999 3869 4295 107
Size: 2892 870 426 3467
Fragments arranged by size:
3467 2892 870 426
NgoMIV G'CCGG_C
Cuts at: 5834 5834
Size: 7655
NheI G'CTAG_C
Cuts at: 1087 1722 1087
Size: 635 7020
NsiI A_TGCA'T
Cuts at: 1527 1527
Size: 7655
NspI r_CATG'y
Cuts at: 447 4708 5208 6811 447
Size: 4261 500 1603 1291
Fragments arranged by size:
4261 1603 1291 500
PasI CC'CwG_GG
Cuts at: 4555 4555
Size: 7655
PciI A'CATG_T
Cuts at: 6807 6807
Size: 7655
PflMI CCAn_nnn'nTGG
Cuts at: 1071 1758 2940 3820 4425 1071
Size: 687 1182 880 605 4301
Fragments arranged by size:
4301 1182 880 687 605
PfoI T'CCnGG_A
Cuts at: 373 2442 2627 3998 373
Size: 2069 185 1371 4030
Fragments arranged by size:
4030 2069 1371 185
PmeI GTTT'AAAC
Cuts at: 4654 4654
Size: 7655
PmlI CAC'GTG
Cuts at: 1944 1944
Size: 7655
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4631 4663 6101 6133 4631
Size: 32 1438 32 6153
Fragments arranged by size:
6153 1438 32 32
PpuMI rG'GwC_Cy
Cuts at: 207 1230 2656 2757 3369 207
Size: 1023 1426 101 612 4493
Fragments arranged by size:
4493 1426 1023 612 101
PshAI GACnn'nnGTC
Cuts at: 1709 1709
Size: 7655
PspOMI G'GGCC_C
Cuts at: 3258 3356 4067 4198 3258
Size: 98 711 131 6715
Fragments arranged by size:
6715 711 131 98
PstI C_TGCA'G
Cuts at: 500 693 2646 2756 3746 4702 5387 500
Size: 193 1953 110 990 956 685 2768
Fragments arranged by size:
2768 1953 990 956 685 193 110
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7655
PvuII CAG'CTG
Cuts at: 1865 1988 2144 2282 2730 3125 3217 3641 3817
Size: 123 156 138 448 395 92 424 176
Cuts at: 3817 4371 5080 5440 1865
Size: 554 709 360 4080
Fragments arranged by size:
4080 709 554 448 424 395 360 176
156 138 123 92
RsrII CG'GwC_CG
Cuts at: 5850 5850
Size: 7655
SacI G_AGCT'C
Cuts at: 1399 2308 3076 4385 4669 1399
Size: 909 768 1309 284 4385
Fragments arranged by size:
4385 1309 909 768 284
SacII CC_GC'GG
Cuts at: 4389 4389
Size: 7655
SalI G'TCGA_C
Cuts at: 4692 4692
Size: 7655
SanDI GG'GwC_CC
Cuts at: 1230 1230
Size: 7655
SapI GCTCTTCn'nnn_
Cuts at: 3203 5678 5888 3203
Size: 2475 210 4970
Fragments arranged by size:
4970 2475 210
SbfI CC_TGCA'GG
Cuts at: 2756 4702 2756
Size: 1946 5709
ScaI AGT'ACT
Cuts at: 418 7172 418
Size: 6754 901
SexAI A'CCwGG_T
Cuts at: 3992 3992
Size: 7655
SfcI C'TryA_G
Cuts at: 33 496 689 1783 2642 2752 3271 3742 4698
Size: 463 193 1094 859 110 519 471 956
Cuts at: 4698 5383 6351 6542 33
Size: 685 968 191 1146
Fragments arranged by size:
1146 1094 968 956 859 685 519 471
463 193 191 110
SfoI GGC'GCC
Cuts at: 2299 5087 5335 2299
Size: 2788 248 4619
Fragments arranged by size:
4619 2788 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7655
SmaI CCC'GGG
Cuts at: 3037 4677 3037
Size: 1640 6015
SmlI C'TyrA_G
Cuts at: 2821 3459 3929 4810 5725 6439 6701 2821
Size: 638 470 881 915 714 262 3775
Fragments arranged by size:
3775 915 881 714 638 470 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7655
SphI G_CATG'C
Cuts at: 4708 5208 4708
Size: 500 7155
SspI AAT'ATT
Cuts at: 2025 2025
Size: 7655
StuI AGG'CCT
Cuts at: 1327 1327
Size: 7655
StyI C'CwwG_G
Cuts at: 107 218 227 2999 3869 4107 4171 4245 4295
Size: 111 9 2772 870 238 64 74 50
Cuts at: 4295 4789 5143 107
Size: 494 354 2619
Fragments arranged by size:
2772 2619 870 494 354 238 111 74
64 50 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 903 1492 2033 2544 2967 5238 5371 5830 6039
Size: 589 541 511 423 2271 133 459 209
Cuts at: 6039 903
Size: 2519
Fragments arranged by size:
2519 2271 589 541 511 459 423 209
133
TatI w'GTAC_w
Cuts at: 416 548 1193 1253 1775 1994 3420 4123 7170
Size: 132 645 60 522 219 1426 703 3047
Cuts at: 7170 416
Size: 901
Fragments arranged by size:
3047 1426 901 703 645 522 219 132
60
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 236 268 1795 1827 1904 1936 2504 2536 7172
Size: 32 1527 32 77 32 568 32 4636
Cuts at: 7172 7204 236
Size: 32 687
Fragments arranged by size:
4636 1527 687 568 77 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 5452 5452
Size: 7655
XbaI T'CTAG_A
Cuts at: 4686 4686
Size: 7655
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 225 2401 2544 3867 4192 4426 5150 225
Size: 2176 143 1323 325 234 724 2730
Fragments arranged by size:
2730 2176 1323 724 325 234 143
XmaI C'CCGG_G
Cuts at: 3035 4675 3035
Size: 1640 6015
XmnI GAAnn'nnTTC
Cuts at: 2504 2504
Size: 7655
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AloI AscI
BaeI BsiWI BspEI BssHII BstZ17I ClaI FseI FspAI
HindIII HpaI MfeI NdeI NotI NruI PacI PsiI
PspXI PsrI SfiI SgrAI SnaBI SrfI SwaI XhoI
ZraI