Restriction map for pF1KA0596
 (Linear) (Six-base) MAPSORT of: KIAA0596.seq  Check: 300  from: 1  to: 4542



KIAA0596.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:54  ..

AccI GT'mk_AC

Cuts at:      0    1190    2864    4542 
   Size:       1190    1674    1678

  Fragments arranged by size: 

                1678    1674    1190
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1507    1564    2408    3065    4370    4542 
   Size:       1507      57     844     657    1305     172

  Fragments arranged by size: 

                1507    1305     844     657     172      57
 
AflIII A'CryG_T

Cuts at:      0     482    4542 
   Size:        482    4060
 
AleI CACnn'nnGTG

Cuts at:      0     706    4542 
   Size:        706    3836
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     356    4542 
   Size:        356    4186
 
AlwNI CAG_nnn'CTG

Cuts at:      0     148    1074    1377    1530    1686    2229    2533    2993 
   Size:        148     926     303     153     156     543     304     460

Cuts at:   2993    3017    3493    3533    4103    4116    4401    4479    4542 
   Size:         24     476      40     570      13     285      78      63

  Fragments arranged by size: 

                 926     570     543     476     460     304     303     285
                 156     153     148      78      63      40      24      13
            
 
ApaI G_GGCC'C

Cuts at:      0    3154    3252    3963    4094    4542 
   Size:       3154      98     711     131     448

  Fragments arranged by size: 

                3154     711     448     131      98
 
ApaLI G'TGCA_C

Cuts at:      0    1513    3595    4542 
   Size:       1513    2082     947

  Fragments arranged by size: 

                2082    1513     947
 
ApoI r'AATT_y

Cuts at:      0     601    2128    4542 
   Size:        601    1527    2414

  Fragments arranged by size: 

                2414    1527     601
 
AvaI C'yCGr_G

Cuts at:      0    1195    2927    3665    4542 
   Size:       1195    1732     738     877

  Fragments arranged by size: 

                1732    1195     877     738
 
AvrII C'CTAG_G

Cuts at:      0    3999    4063    4542 
   Size:       3999      64     479

  Fragments arranged by size: 

                3999     479      64
 
BamHI G'GATC_C

Cuts at:      0    1428    4542 
   Size:       1428    3114
 
BanI G'GyrC_C

Cuts at:      0     898    2189    2309    3384    3421    3606    4048    4542 
   Size:        898    1291     120    1075      37     185     442     494

  Fragments arranged by size: 

                1291    1075     898     494     442     185     120      37
            
 
BanII G_rGCy'C

Cuts at:      0    1291    1877    2200    2968    3068    3154    3252    3540 
   Size:       1291     586     323     768     100      86      98     288

Cuts at:   3540    3697    3882    3963    4094    4277    4542 
   Size:        157     185      81     131     183     265

  Fragments arranged by size: 

                1291     768     586     323     288     265     185     183
                 157     131     100      98      86      81
 
BbeI G_GCGC'C

Cuts at:      0    2193    4542 
   Size:       2193    2349
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1852    3053    4180    4542 
   Size:       1852    1201    1127     362

  Fragments arranged by size: 

                1852    1201    1127     362
 
BbvCI CC'TCA_GC

Cuts at:      0     103     814    1327    3110    4338    4542 
   Size:        103     711     513    1783    1228     204

  Fragments arranged by size: 

                1783    1228     711     513     204     103
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2426    2460    3350    3384    4542 
   Size:       2426      34     890      34    1158

  Fragments arranged by size: 

                2426    1158     890      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1040    2889    4542 
   Size:       1040    1849    1653

  Fragments arranged by size: 

                1849    1653    1040
 
BclI T'GATC_A

Cuts at:      0    1751    4542 
   Size:       1751    2791
 
BfrBI ATG'CAT

Cuts at:      0    1417    4542 
   Size:       1417    3125
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2751    4542 
   Size:       2751    1791
 
BglII A'GATC_T

Cuts at:      0      49    2829    4542 
   Size:         49    2780    1713

  Fragments arranged by size: 

                2780    1713      49
 
Bme1580I G_kGCm'C

Cuts at:      0     335     653    1504    1517    2502    3154    3252    3599 
   Size:        335     318     851      13     985     652      98     347

Cuts at:   3599    3963    4051    4094    4110    4542 
   Size:        364      88      43      16     432

  Fragments arranged by size: 

                 985     851     652     432     364     347     335     318
                  98      88      43      16      13
 
BmgBI CAC'GTC

Cuts at:      0     360     755    4542 
   Size:        360     395    3787

  Fragments arranged by size: 

                3787     395     360
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1226    2493    3247    3582    4542 
   Size:       1226    1267     754     335     960

  Fragments arranged by size: 

                1267    1226     960     754     335
 
BmtI G_CTAG'C

Cuts at:      0     983    1618    4542 
   Size:        983     635    2924

  Fragments arranged by size: 

                2924     983     635
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     118    4509    4542 
   Size:        118    4391      33

  Fragments arranged by size: 

                4391     118      33
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     170     341    1031    1197    1418    1600    1823    2906 
   Size:        170     171     690     166     221     182     223    1083

Cuts at:   2906    3170    3227    3455    3562    4267    4504    4542 
   Size:        264      57     228     107     705     237      38

  Fragments arranged by size: 

                1083     705     690     264     237     228     223     221
                 182     171     170     166     107      57      38
 
Bpu10I CC'TnA_GC

Cuts at:      0     103     666     814     937    1327    2194    3110    3680 
   Size:        103     563     148     123     390     867     916     570

Cuts at:   3680    4216    4338    4542 
   Size:        536     122     204

  Fragments arranged by size: 

                 916     867     570     563     536     390     204     148
                 123     122     103
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2734    3336    3806    4542 
   Size:       2734     602     470     736

  Fragments arranged by size: 

                2734     736     602     470
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    3366    4542 
   Size:       3366    1176
 
BsaAI yAC'GTr

Cuts at:      0    1836    4542 
   Size:       1836    2706
 
BsaBI GATnn'nnATC

Cuts at:      0      54    2209    4542 
   Size:         54    2155    2333

  Fragments arranged by size: 

                2333    2155      54
 
BsaHI Gr'CG_yC

Cuts at:      0    2190    4542 
   Size:       2190    2352
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      91     121     385     415    1687    1717    1796    1826 
   Size:         91      30     264      30    1272      30      79      30

Cuts at:   1826    4542 
   Size:       2716

  Fragments arranged by size: 

                2716    1272     264      91      79      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1242    1415    2061    3078    3151    3339    4357    4542 
   Size:       1242     173     646    1017      73     188    1018     185

  Fragments arranged by size: 

                1242    1018    1017     646     188     185     173      73
            
 
BseYI C'CCAG_C

Cuts at:      0     132     198     657     675    1876    2452    2457    2526 
   Size:        132      66     459      18    1201     576       5      69

Cuts at:   2526    2567    3472    3479    3533    3705    3871    4093    4109 
   Size:         41     905       7      54     172     166     222      16

Cuts at:   4109    4361    4414    4542 
   Size:        252      53     128

  Fragments arranged by size: 

                1201     905     576     459     252     222     172     166
                 132     128      69      66      54      53      41      18
                  16       7       5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     913    1834    2020    2825    2935    3277    4542 
   Size:        913     921     186     805     110     342    1265

  Fragments arranged by size: 

                1265     921     913     805     342     186     110
 
BsiHKAI G_wGCw'C

Cuts at:      0     371     398    1291    1517    2200    2968    3038    3061 
   Size:        371      27     893     226     683     768      70      23

Cuts at:   3061    3441    3599    4277    4542 
   Size:        380     158     678     265

  Fragments arranged by size: 

                 893     768     683     678     380     371     265     226
                 158      70      27      23
 
BsmI GAATG_Cn'

Cuts at:      0     645    1026    3934    4198    4542 
   Size:        645     381    2908     264     344

  Fragments arranged by size: 

                2908     645     381     344     264
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    3520    3728    4542 
   Size:       3520     208     814

  Fragments arranged by size: 

                3520     814     208
 
Bsp1286I G_dGCh'C

Cuts at:      0     335     371     398     653    1291    1504    1517    1877 
   Size:        335      36      27     255     638     213      13     360

Cuts at:   1877    2200    2502    2968    3038    3061    3068    3154    3252 
   Size:        323     302     466      70      23       7      86      98

Cuts at:   3252    3441    3540    3599    3697    3882    3963    4051    4094 
   Size:        189      99      59      98     185      81      88      43

Cuts at:   4094    4110    4277    4542 
   Size:         16     167     265

  Fragments arranged by size: 

                 638     466     360     335     323     302     265     255
                 213     189     185     167      99      98      98      88
                  86      81      70      59      43      36      27      23
                  16      13       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     935    1255    2847    3249    4542 
   Size:        935     320    1592     402    1293

  Fragments arranged by size: 

                1592    1293     935     402     320
 
BsrBI CCG'CTC

Cuts at:      0     665    1448    4542 
   Size:        665     783    3094

  Fragments arranged by size: 

                3094     783     665
 
BsrDI GCAATG_nn'

Cuts at:      0     974    1040    2024    3423    4542 
   Size:        974      66     984    1399    1119

  Fragments arranged by size: 

                1399    1119     984     974      66
 
BsrFI r'CCGG_y

Cuts at:      0    1634    2306    2340    4542 
   Size:       1634     672      34    2202

  Fragments arranged by size: 

                2202    1634     672      34
 
BsrGI T'GTAC_A

Cuts at:      0    1085    4542 
   Size:       1085    3457
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    3017    4542 
   Size:       3017    1525
 
BstEII G'GTnAC_C

Cuts at:      0     114    4542 
   Size:        114    4428
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1134    3549    3762    4188    4542 
   Size:       1134    2415     213     426     354

  Fragments arranged by size: 

                2415    1134     426     354     213
 
BstYI r'GATC_y

Cuts at:      0      49    1428    2018    2599    2610    2829    4542 
   Size:         49    1379     590     581      11     219    1713

  Fragments arranged by size: 

                1713    1379     590     581     219      49      11
 
Bsu36I CC'TnA_GG

Cuts at:      0     759    2013    2073    2756    4542 
   Size:        759    1254      60     683    1786

  Fragments arranged by size: 

                1786    1254     759     683      60
 
BtgI C'CryG_G

Cuts at:      0    2510    2891    3761    4187    4278    4514    4542 
   Size:       2510     381     870     426      91     236      28

  Fragments arranged by size: 

                2510     870     426     381     236      91      28
 
BtsI GCAGTG_nn'

Cuts at:      0     278     551     578     849    1338    1709    1787    1944 
   Size:        278     273      27     271     489     371      78     157

Cuts at:   1944    2404    3655    3907    4258    4304    4427    4542 
   Size:        460    1251     252     351      46     123     115

  Fragments arranged by size: 

                1251     489     460     371     351     278     273     271
                 252     157     123     115      78      46      27
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     824     859    2079    2114    4542 
   Size:        824      35    1220      35    2428

  Fragments arranged by size: 

                2428    1220     824      35      35
 
DraI TTT'AAA

Cuts at:      0    1962    4542 
   Size:       1962    2580
 
DraIII CAC_nnn'GTG

Cuts at:      0    1836    1839    1975    4317    4542 
   Size:       1836       3     136    2342     225

  Fragments arranged by size: 

                2342    1836     225     136       3
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     475     546    4542 
   Size:        475      71    3996

  Fragments arranged by size: 

                3996     475      71
 
EaeI y'GGCC_r

Cuts at:      0     380    2087    2100    2231    2669    3227    3551    3764 
   Size:        380    1707      13     131     438     558     324     213

Cuts at:   3764    4542 
   Size:        778

  Fragments arranged by size: 

                1707     778     558     438     380     324     213     131
                  13
 
EarI CTCTTCn'nnn_

Cuts at:      0    1009    2553    2937    3095    3119    3128    3297    4138 
   Size:       1009    1544     384     158      24       9     169     841

Cuts at:   4138    4389    4542 
   Size:        251     153

  Fragments arranged by size: 

                1544    1009     841     384     251     169     158     153
                  24       9
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     748    1258    4542 
   Size:        748     510    3284

  Fragments arranged by size: 

                3284     748     510
 
EcoICRI GAG'CTC

Cuts at:      0    1289    2198    2966    4275    4542 
   Size:       1289     909     768    1309     267

  Fragments arranged by size: 

                1309    1289     909     768     267
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     170     341    1031    1197    1418    1507    1564    1600 
   Size:        170     171     690     166     221      89      57      36

Cuts at:   1600    1823    2408    2906    3065    3170    3227    3455    3562 
   Size:        223     585     498     159     105      57     228     107

Cuts at:   3562    4267    4370    4504    4542 
   Size:        705     103     134      38

  Fragments arranged by size: 

                 705     690     585     498     228     223     221     171
                 170     166     159     134     107     105     103      89
                  57      57      38      36
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    3647    4219    4542 
   Size:       3647     572     323

  Fragments arranged by size: 

                3647     572     323
 
EcoO109I rG'GnC_Cy

Cuts at:      0      99    1122    1374    2316    2548    2649    2709    3151 
   Size:         99    1023     252     942     232     101      60     442

Cuts at:   3151    3248    3249    3261    3639    3960    4141    4476    4542 
   Size:         97       1      12     378     321     181     335      66

  Fragments arranged by size: 

                1023     942     442     378     335     321     252     232
                 181     101      99      97      66      60      12       1
            
 
EcoRV GAT'ATC

Cuts at:      0    3773    4542 
   Size:       3773     769
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     135    2413    4002    4542 
   Size:        135    2278    1589     540

  Fragments arranged by size: 

                2278    1589     540     135
 
FspI TGC'GCA

Cuts at:      0      70    1793    4542 
   Size:         70    1723    2749

  Fragments arranged by size: 

                2749    1723      70
 
HaeII r_GCGC'y

Cuts at:      0     437    2193    2568    4542 
   Size:        437    1756     375    1974

  Fragments arranged by size: 

                1974    1756     437     375
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      24      56      91     123     163     195    1041    1073 
   Size:         24      32      35      32      40      32     846      32

Cuts at:   1073    1681    1713    2056    2088    2211    2243    2330    2362 
   Size:        608      32     343      32     123      32      87      32

Cuts at:   2362    3070    3102    3500    3532    3663    3695    4366    4398 
   Size:        708      32     398      32     131      32     671      32

Cuts at:   4398    4542 
   Size:        144

  Fragments arranged by size: 

                 846     708     671     608     398     343     144     131
                 123      87      40      35      32      32      32      32
                  32      32      32      32      32      32      32      32
                  24
 
HincII GTy'rAC

Cuts at:      0      17     480    4542 
   Size:         17     463    4062

  Fragments arranged by size: 

                4062     463      17
 
KasI G'GCGC_C

Cuts at:      0    2189    4542 
   Size:       2189    2353
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     542    1406    4333    4542 
   Size:        542     864    2927     209

  Fragments arranged by size: 

                2927     864     542     209
 
MscI TGG'CCA

Cuts at:      0    2089    2102    2671    3229    3553    3766    4542 
   Size:       2089      13     569     558     324     213     776

  Fragments arranged by size: 

                2089     776     569     558     324     213      13
 
MslI CAynn'nnrTG

Cuts at:      0     401     508     706     859    1354    1867    1996    2122 
   Size:        401     107     198     153     495     513     129     126

Cuts at:   2122    2158    2182    2215    4542 
   Size:         36      24      33    2327

  Fragments arranged by size: 

                2327     513     495     401     198     153     129     126
                 107      36      33      24
 
MspA1I CmG'CkG

Cuts at:      0    1757    1880    2036    2174    2253    2622    3017    3109 
   Size:       1757     123     156     138      79     369     395      92

Cuts at:   3109    3533    3709    4237    4263    4280    4542 
   Size:        424     176     528      26      17     262

  Fragments arranged by size: 

                1757     528     424     395     369     262     176     156
                 138     123      92      79      26      17
 
NarI GG'CG_CC

Cuts at:      0    2190    4542 
   Size:       2190    2352
 
NcoI C'CATG_G

Cuts at:      0    2891    3761    4187    4542 
   Size:       2891     870     426     355

  Fragments arranged by size: 

                2891     870     426     355
 
NheI G'CTAG_C

Cuts at:      0     979    1614    4542 
   Size:        979     635    2928

  Fragments arranged by size: 

                2928     979     635
 
NsiI A_TGCA'T

Cuts at:      0    1419    4542 
   Size:       1419    3123
 
NspI r_CATG'y

Cuts at:      0     339    4542 
   Size:        339    4203
 
PasI CC'CwG_GG

Cuts at:      0    4447    4542 
   Size:       4447      95
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     963    1650    2832    3712    4317    4542 
   Size:        963     687    1182     880     605     225

  Fragments arranged by size: 

                1182     963     880     687     605     225
 
PfoI T'CCnGG_A

Cuts at:      0     265    2334    2519    3890    4542 
   Size:        265    2069     185    1371     652

  Fragments arranged by size: 

                2069    1371     652     265     185
 
PmlI CAC'GTG

Cuts at:      0    1836    4542 
   Size:       1836    2706
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    4523    4542 
   Size:       4523      19
 
PpuMI rG'GwC_Cy

Cuts at:      0      99    1122    2548    2649    3261    4542 
   Size:         99    1023    1426     101     612    1281

  Fragments arranged by size: 

                1426    1281    1023     612     101      99
 
PshAI GACnn'nnGTC

Cuts at:      0    1601    4542 
   Size:       1601    2941
 
PspOMI G'GGCC_C

Cuts at:      0    3150    3248    3959    4090    4542 
   Size:       3150      98     711     131     452

  Fragments arranged by size: 

                3150     711     452     131      98
 
PstI C_TGCA'G

Cuts at:      0     392     585    2538    2648    3638    4542 
   Size:        392     193    1953     110     990     904

  Fragments arranged by size: 

                1953     990     904     392     193     110
 
PvuII CAG'CTG

Cuts at:      0    1757    1880    2036    2174    2622    3017    3109    3533 
   Size:       1757     123     156     138     448     395      92     424

Cuts at:   3533    3709    4263    4542 
   Size:        176     554     279

  Fragments arranged by size: 

                1757     554     448     424     395     279     176     156
                 138     123      92
 
SacI G_AGCT'C

Cuts at:      0    1291    2200    2968    4277    4542 
   Size:       1291     909     768    1309     265

  Fragments arranged by size: 

                1309    1291     909     768     265
 
SacII CC_GC'GG

Cuts at:      0    4281    4542 
   Size:       4281     261
 
SanDI GG'GwC_CC

Cuts at:      0    1122    4542 
   Size:       1122    3420
 
SapI GCTCTTCn'nnn_

Cuts at:      0    3095    4542 
   Size:       3095    1447
 
SbfI CC_TGCA'GG

Cuts at:      0    2648    4542 
   Size:       2648    1894
 
ScaI AGT'ACT

Cuts at:      0     310    4542 
   Size:        310    4232
 
SexAI A'CCwGG_T

Cuts at:      0    3884    4542 
   Size:       3884     658
 
SfcI C'TryA_G

Cuts at:      0     388     581    1675    2534    2644    3163    3634    4542 
   Size:        388     193    1094     859     110     519     471     908

  Fragments arranged by size: 

                1094     908     859     519     471     388     193     110
            
 
SfoI GGC'GCC

Cuts at:      0    2191    4542 
   Size:       2191    2351
 
SmaI CCC'GGG

Cuts at:      0    2929    4542 
   Size:       2929    1613
 
SmlI C'TyrA_G

Cuts at:      0    2713    3351    3821    4542 
   Size:       2713     638     470     721

  Fragments arranged by size: 

                2713     721     638     470
 
SspI AAT'ATT

Cuts at:      0    1917    4542 
   Size:       1917    2625
 
StuI AGG'CCT

Cuts at:      0    1219    4542 
   Size:       1219    3323
 
StyI C'CwwG_G

Cuts at:      0     110     119    2891    3761    3999    4063    4137    4187 
   Size:        110       9    2772     870     238      64      74      50

Cuts at:   4187    4542 
   Size:        355

  Fragments arranged by size: 

                2772     870     355     238     110      74      64      50
                   9
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     795    1384    1925    2436    2859    4542 
   Size:        795     589     541     511     423    1683

  Fragments arranged by size: 

                1683     795     589     541     511     423
 
TatI w'GTAC_w

Cuts at:      0     308     440    1085    1145    1667    1886    3312    4015 
   Size:        308     132     645      60     522     219    1426     703

Cuts at:   4015    4542 
   Size:        527

  Fragments arranged by size: 

                1426     703     645     527     522     308     219     132
                  60
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     128     160    1687    1719    1796    1828    2396    2428 
   Size:        128      32    1527      32      77      32     568      32

Cuts at:   2428    4542 
   Size:       2114

  Fragments arranged by size: 

                2114    1527     568     128      77      32      32      32
                  32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     117    2293    2436    3759    4084    4318    4542 
   Size:        117    2176     143    1323     325     234     224

  Fragments arranged by size: 

                2176    1323     325     234     224     143     117
 
XmaI C'CCGG_G

Cuts at:      0    2927    4542 
   Size:       2927    1615
 
XmnI GAAnn'nnTTC

Cuts at:      0    2396    4542 
   Size:       2396    2146
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AleI     AlfI    AlwNI     ApaI    ApaLI
     ApoI     AvaI    AvrII    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI    CspCI
     DraI   DraIII     DrdI     EaeI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRV     FalI     FspI    HaeII    Hin4I   HincII
     KasI     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NheI
     NsiI     NspI     PasI    PflMI     PfoI     PmlI     PpiI    PpuMI
    PshAI   PspOMI     PstI    PvuII     SacI    SacII    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfoI     SmaI     SmlI     SspI
     StuI     StyI    TaqII     TatI     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AloI     AscI     AseI    AsiSI     BaeI     BlpI    BsaWI    BsiEI
    BsiWI    BspEI    BspHI   BssHII    BssSI    BstBI  BstZ17I    BtgZI
     ClaI     EagI    EcoRI     FseI    FspAI  HindIII     HpaI     KpnI
     MfeI     MluI     NaeI     NdeI   NgoMIV     NotI     NruI     PacI
     PciI     PmeI     PsiI    PspXI     PsrI     PvuI    RsrII     SalI
     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SwaI
  Tth111I     XbaI     XhoI     ZraI
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