Restriction map for pF1KA0072
##################################
# NO CUT ENZYMES (  127 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AflIII      AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AviII       AvrII       
BalI        BanI        BbeI        BbsI        BbvCI       
BcgI        BclI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BpuAI       BsaBI       BsaHI       
BsaI        BseAI       BsgI        BsiEI       BsiWI       
BsmAI       BsmBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrGI       BssSI       Bst1107I    
BstBI       BstXI       BstZ17I     CelII       ClaI        
CpoI        DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoRI       EcoRV       
EcoT14I     EcoT22I     FbaI        FseI        FspI        
HgaI        Hin1I       HpaI        KasI        KpnI        
KspI        MamI        MluI        MluNI       MroI        
MscI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PleI        PmeI        PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        Tth111I     XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccI          946  GTAGAC               GT^[AC][GT]AC
AlwNI         631  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          249  CCTCAGG              CC^T[ACGT]AGG
Asp700I       226  GAACCGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI        1189  GAGCTC               G[AT]GC[AT]^C
AvaI         1175  CTCGGG               C^[CT]CG[GA]G
BbrPI         235  CACGTG               CAC^GTG
BciVI         193  GTATCC               GTATCC(6/5)
BcnI          242  CCCGG                CC^[GC]GG
BglII         863  AGATCT               A^GATCT
BsaAI         235  CACGTG               [CT]AC^GT[GA]
BseRI          58  GAGGAG               GAGGAG(10/8)
BsiHKAI      1189  GAGCTC               G[AT]GC[AT]^C
BsmFI         270  GGGAC                GGGAC(10/14)
BsmI         1053  GAATGC               GAATGC(1/-1)
BsoBI        1175  CTCGGG               C^[CT]CG[GA]G
BspMI         260  ACCTGC               ACCTGC(4/8)
BsrDI        1260  CATTGC               GCAATG(2/0)
BssHII         68  GCGCGC               G^CGCGC
BstAPI         87  GCAGGCAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        624  GGTAACC              G^GT[ACGT]ACC
BstPI         624  GGTAACC              G^GT[ACGT]ACC
Bsu36I        249  CCTCAGG              CC^T[ACGT]AGG
DraI          597  TTTAAA               TTT^AAA
DraII         920  AGGACCC              [GA]G^G[ACGT]CC[CT]
DraIII         76  CACTCCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         1197  CCGTGG               C^C[GA][CT]GG
EaeI         1256  CGGCCA               [CT]^GGCC[GA]
Eco81I        249  CCTCAGG              CC^T[ACGT]AGG
EcoO109I      920  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoO65I       624  GGTAACC              G^GT[ACGT]ACC
FauI          141  CCCGC                CCCGC(4/6)
HincII        256  GTTGAC               GT[CT]^[GA]AC
HindII        256  GTTGAC               GT[CT]^[GA]AC
HindIII      1349  AAGCTT               A^AGCTT
MaeII         236  ACGT                 A^CGT
MfeI         1403  CAATTG               C^AATTG
MslI          280  CACCCTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I         52  CCGCTG               C[AC]G^C[GT]G
MunI         1403  CAATTG               C^AATTG
NciI          242  CCCGG                CC^[GC]GG
PmaCI         235  CACGTG               CAC^GTG
PmlI          235  CACGTG               CAC^GTG
PpuMI         920  AGGACCC              [GA]G^G[AT]CC[CT]
SacI         1189  GAGCTC               GAGCT^C
SmlI          734  CTCAAG               C^T[CT][GA]AG
SpeI         1374  ACTAGT               A^CTAGT
TaqI          543  TCGA                 T^CGA
TthHB8I       543  TCGA                 T^CGA
XcmI          611  CCACAAGTGGATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          226  GAACCGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccII          67  CGCG                 CG^CG
               69  CGCG                
AcsI          336  AAATTC               [GA]^AATT[CT]
              755  AAATTT              
AfaI          608  GTAC                 GT^AC
             1107  GTAC                
AgeI          228  ACCGGT               A^CCGGT
             1328  ACCGGT              
ApoI          336  AAATTC               [GA]^AATT[CT]
              755  AAATTT              
AvaII         921  GGACC                G^G[AT]CC
             1326  GGACC               
BamHI         967  GGATCC               G^GATCC
             1179  GGATCC              
BanII        1189  GAGCTC               G[GA]GC[CT]^C
             1277  GGGCTC              
BfaI          797  CTAG                 C^TAG
             1375  CTAG                
BmyI         1189  GAGCTC               G[AGT]GC[ACT]^C
             1277  GGGCTC              
BsaWI         228  ACCGGT               [AT]^CCGG[AT]
             1328  ACCGGT              
Bsp1286I     1189  GAGCTC               G[AGT]GC[ACT]^C
             1277  GGGCTC              
BsrBI          97  CCGCTC               CCGCTC(-3/-3)
              142  CCGCTC              
BsrFI         228  ACCGGT               [GA]^CCGG[CT]
             1328  ACCGGT              
BstUI          67  CGCG                 CG^CG
               69  CGCG                
Cfr10I        228  ACCGGT               [GA]^CCGG[CT]
             1328  ACCGGT              
HaeII          18  GGCGCT               [GA]GCGC^[CT]
               48  AGCGCC              
MaeI          797  CTAG                 C^TAG
             1375  CTAG                
MvnI           67  CGCG                 CG^CG
               69  CGCG                
PflMI           9  CCAGGCTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              216  CCAAGCTATGG         
PinAI         228  ACCGGT               A^CCGGT
             1328  ACCGGT              
PstI           85  CTGCAG               CTGCA^G
              262  CTGCAG              
RsaI          608  GTAC                 GT^AC
             1107  GTAC                
SapI          144  GCTCTTC              GCTCTTC(1/4)
             1191  GCTCTTC             
Tsp45I        585  GTCAC                ^GT[GC]AC
              691  GTGAC               
Van91I          9  CCAGGCTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              216  CCAAGCTATGG         


#########################################
# 3 OR MORE CUT ENZYMES (   54 enzymes) #
#########################################

AciI        AluI        AlwI        BbvI        BpmI        
BsaJI       BsiYI       BslI        BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       EarI        EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeIII      MboI        MboII       MflI        
MnlI        MseI        MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        TfiI        Tru9I       
TseI        Tsp509I     TspRI       XhoII       
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