Restriction map for pF1KA0013
 (Linear) (Six-base) MAPSORT of: KIAA0013.seq  Check: 624  from: 1  to: 3069



KIAA0013.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:54  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1177    1686    3069 
   Size:       1177     509    1383

  Fragments arranged by size: 

                1383    1177     509
 
AccI GT'mk_AC

Cuts at:      0    2830    3069 
   Size:       2830     239
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     595    3069 
   Size:        595    2474
 
AflII C'TTAA_G

Cuts at:      0     361    2491    3069 
   Size:        361    2130     578

  Fragments arranged by size: 

                2130     578     361
 
AflIII A'CryG_T

Cuts at:      0    2302    2368    3069 
   Size:       2302      66     701

  Fragments arranged by size: 

                2302     701      66
 
AgeI A'CCGG_T

Cuts at:      0    1095    3069 
   Size:       1095    1974
 
AlwNI CAG_nnn'CTG

Cuts at:      0      15      42     271    2386    3069 
   Size:         15      27     229    2115     683

  Fragments arranged by size: 

                2115     683     229      27      15
 
ApoI r'AATT_y

Cuts at:      0     886    1312    2146    3069 
   Size:        886     426     834     923

  Fragments arranged by size: 

                 923     886     834     426
 
AseI AT'TA_AT

Cuts at:      0    2534    3069 
   Size:       2534     535
 
AvrII C'CTAG_G

Cuts at:      0    2329    3069 
   Size:       2329     740
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1082    1115    2991    3024    3069 
   Size:       1082      33    1876      33      45

  Fragments arranged by size: 

                1876    1082      45      33      33
 
BanII G_rGCy'C

Cuts at:      0     392    3069 
   Size:        392    2677
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     236     475     632     852    3069 
   Size:        236     239     157     220    2217

  Fragments arranged by size: 

                2217     239     236     220     157
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      73     107    3069 
   Size:         73      34    2962

  Fragments arranged by size: 

                2962      73      34
 
BclI T'GATC_A

Cuts at:      0    1178    2424    3069 
   Size:       1178    1246     645

  Fragments arranged by size: 

                1246    1178     645
 
BfrBI ATG'CAT

Cuts at:      0     703    3069 
   Size:        703    2366
 
BglII A'GATC_T

Cuts at:      0    1297    2852    3069 
   Size:       1297    1555     217

  Fragments arranged by size: 

                1555    1297     217
 
BlpI GC'TnA_GC

Cuts at:      0     977    3069 
   Size:        977    2092
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2787    3069 
   Size:       2787     282
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1508    2136    3069 
   Size:       1508     628     933

  Fragments arranged by size: 

                1508     933     628
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     905     999    1651    3069 
   Size:        905      94     652    1418

  Fragments arranged by size: 

                1418     905     652      94
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     775    2967    3069 
   Size:        775    2192     102

  Fragments arranged by size: 

                2192     775     102
 
BsaWI w'CCGG_w

Cuts at:      0    1095    1550    3069 
   Size:       1095     455    1519

  Fragments arranged by size: 

                1519    1095     455
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     851     881    2586    2616    3069 
   Size:        851      30    1705      30     453

  Fragments arranged by size: 

                1705     851     453      30      30
 
BseYI C'CCAG_C

Cuts at:      0     399     938    3069 
   Size:        399     539    2131

  Fragments arranged by size: 

                2131     539     399
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     317    2273    3069 
   Size:        317    1956     796

  Fragments arranged by size: 

                1956     796     317
 
BsiEI CG_ry'CG

Cuts at:      0      93    3069 
   Size:         93    2976
 
BsiHKAI G_wGCw'C

Cuts at:      0     879    2637    3069 
   Size:        879    1758     432

  Fragments arranged by size: 

                1758     879     432
 
BsmI GAATG_Cn'

Cuts at:      0    2820    3069 
   Size:       2820     249
 
Bsp1286I G_dGCh'C

Cuts at:      0     392     879    2637    3069 
   Size:        392     487    1758     432

  Fragments arranged by size: 

                1758     487     432     392
 
BspHI T'CATG_A

Cuts at:      0    2901    3069 
   Size:       2901     168
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1177    1686    3069 
   Size:       1177     509    1383

  Fragments arranged by size: 

                1383    1177     509
 
BsrDI GCAATG_nn'

Cuts at:      0     985    3069 
   Size:        985    2084
 
BsrFI r'CCGG_y

Cuts at:      0    1095    3069 
   Size:       1095    1974
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      42    3069 
   Size:         42    3027
 
BstBI TT'CG_AA

Cuts at:      0    1293    3069 
   Size:       1293    1776
 
BstYI r'GATC_y

Cuts at:      0     268     550    1297    2278    2852    3069 
   Size:        268     282     747     981     574     217

  Fragments arranged by size: 

                 981     747     574     282     268     217
 
Bsu36I CC'TnA_GG

Cuts at:      0    2968    3069 
   Size:       2968     101
 
BtgI C'CryG_G

Cuts at:      0      78     116    3069 
   Size:         78      38    2953

  Fragments arranged by size: 

                2953      78      38
 
BtsI GCAGTG_nn'

Cuts at:      0      91     157    1181    3069 
   Size:         91      66    1024    1888

  Fragments arranged by size: 

                1888    1024      91      66
 
ClaI AT'CG_AT

Cuts at:      0     695    1112    3069 
   Size:        695     417    1957

  Fragments arranged by size: 

                1957     695     417
 
DraI TTT'AAA

Cuts at:      0     891    1409    1712    2456    3069 
   Size:        891     518     303     744     613

  Fragments arranged by size: 

                 891     744     613     518     303
 
EaeI y'GGCC_r

Cuts at:      0    2865    3069 
   Size:       2865     204
 
EarI CTCTTCn'nnn_

Cuts at:      0    1081    2720    3069 
   Size:       1081    1639     349

  Fragments arranged by size: 

                1639    1081     349
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     595    1508    2136    3069 
   Size:        595     913     628     933

  Fragments arranged by size: 

                 933     913     628     595
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1567    2695    2964    2993    3069 
   Size:       1567    1128     269      29      76

  Fragments arranged by size: 

                1567    1128     269      76      29
 
EcoRI G'AATT_C

Cuts at:      0    2146    3069 
   Size:       2146     923
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2898    3069 
   Size:       2898     171
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     688     720    1105    1137    2263    2295    3069 
   Size:        688      32     385      32    1126      32     774

  Fragments arranged by size: 

                1126     774     688     385      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0     198    1607    2446    3069 
   Size:        198    1409     839     623

  Fragments arranged by size: 

                1409     839     623     198
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1518    2525    3069 
   Size:       1518    1007     544

  Fragments arranged by size: 

                1518    1007     544
 
MscI TGG'CCA

Cuts at:      0    2867    3069 
   Size:       2867     202
 
MspA1I CmG'CkG

Cuts at:      0     403    3069 
   Size:        403    2666
 
NsiI A_TGCA'T

Cuts at:      0     705    3069 
   Size:        705    2364
 
NspI r_CATG'y

Cuts at:      0    2306    2372    3069 
   Size:       2306      66     697

  Fragments arranged by size: 

                2306     697      66
 
PciI A'CATG_T

Cuts at:      0    2302    2368    3069 
   Size:       2302      66     701

  Fragments arranged by size: 

                2302     701      66
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     184    3069 
   Size:        184    2885
 
PfoI T'CCnGG_A

Cuts at:      0     734    3069 
   Size:        734    2335
 
PpuMI rG'GwC_Cy

Cuts at:      0    1567    2695    2964    3069 
   Size:       1567    1128     269     105

  Fragments arranged by size: 

                1567    1128     269     105
 
PsiI TTA'TAA

Cuts at:      0    2843    3069 
   Size:       2843     226
 
PstI C_TGCA'G

Cuts at:      0     678    1188    3069 
   Size:        678     510    1881

  Fragments arranged by size: 

                1881     678     510
 
PvuI CG_AT'CG

Cuts at:      0      93    3069 
   Size:         93    2976
 
PvuII CAG'CTG

Cuts at:      0     403    3069 
   Size:        403    2666
 
SfcI C'TryA_G

Cuts at:      0     674    1184    1949    2564    2834    3059    3069 
   Size:        674     510     765     615     270     225      10

  Fragments arranged by size: 

                 765     674     615     510     270     225      10
 
SmlI C'TyrA_G

Cuts at:      0     361     920    1014    1630    2491    3069 
   Size:        361     559      94     616     861     578

  Fragments arranged by size: 

                 861     616     578     559     361      94
 
SpeI A'CTAG_T

Cuts at:      0    1533    3069 
   Size:       1533    1536
 
SspI AAT'ATT

Cuts at:      0     137     593    2736    3069 
   Size:        137     456    2143     333

  Fragments arranged by size: 

                2143     456     333     137
 
StuI AGG'CCT

Cuts at:      0    2334    3069 
   Size:       2334     735
 
StyI C'CwwG_G

Cuts at:      0    1195    2329    2882    3069 
   Size:       1195    1134     553     187

  Fragments arranged by size: 

                1195    1134     553     187
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2816    3069 
   Size:       2816     253
 
TatI w'GTAC_w

Cuts at:      0     681    1155    2299    3069 
   Size:        681     474    1144     770

  Fragments arranged by size: 

                1144     770     681     474
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1782    3069 
   Size:       1782    1287
 
XmnI GAAnn'nnTTC

Cuts at:      0     906    1289    2418    2535    2779    3069 
   Size:        906     383    1129     117     244     290

  Fragments arranged by size: 

                1129     906     383     290     244     117
 

 Enzymes that do cut:

     AarI     AccI     AcuI    AflII   AflIII     AgeI    AlwNI     ApoI
     AseI    AvrII     BaeI    BanII     BbsI     BcgI     BclI    BfrBI
    BglII     BlpI     BplI     BpmI    BpuEI     BsaI    BsaWI    BsaXI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI
    BsrDI    BsrFI   BstAPI    BstBI    BstYI   Bsu36I     BtgI     BtsI
     ClaI     DraI     EaeI     EarI  Eco57MI EcoO109I    EcoRI     FalI
    Hin4I  HindIII     MmeI     MscI   MspA1I     NsiI     NspI     PciI
    PflMI     PfoI    PpuMI     PsiI     PstI     PvuI    PvuII     SfcI
     SmlI     SpeI     SspI     StuI     StyI    TaqII     TatI     XcmI
     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI     AhdI     AleI     AlfI     AloI
     ApaI    ApaLI     AscI    AsiSI     AvaI    BamHI     BanI     BbeI
    BbvCI    BciVI     BglI Bme1580I    BmgBI     BmrI     BmtI   Bpu10I
    BsaAI    BsaBI    BsaHI    BseRI    BsiWI    BsmBI    BspEI    BsrBI
    BsrGI   BssHII    BssSI   BstEII    BstXI  BstZ17I    BtgZI    CspCI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRV     FseI
     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MfeI
     MluI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     PacI     PasI     PmeI     PmlI     PpiI    PshAI
   PspOMI    PspXI     PsrI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SphI     SrfI     SwaI     TstI  Tth111I     XbaI
     XhoI     XmaI     ZraI
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