Restriction map for pF1KB7890
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# NO CUT ENZYMES (  122 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AcyI        AflII       AflIII      AgeI        Alw44I      
AocI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspI        AviII       AvrII       BanI        
BbeI        BbvCI       BciVI       BfrI        BlnI        
BlpI        Bpu1102I    BsaBI       BsaHI       BsaWI       
BseAI       BsgI        BsiEI       BsiWI       BsmBI       
Bsp1407I    BspDI       BspEI       BspLU11I    BspMI       
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DrdI        EagI        EclXI       
Eco52I      Eco81I      EcoO65I     EcoT14I     FauI        
FseI        FspI        HgaI        Hin1I       HincII      
HindII      HpaI        KasI        KpnI        KspI        
MamI        MluI        MroI        MspA1I      MvnI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NspI        PacI        
PaeR7I      PflFI       PinAI       PmeI        PshBI       
PstI        PvuI        PvuII       RsrII       SacI        
SacII       SalI        SapI        SbfI        SfiI        
SfoI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       StuI        StyI        
SwaI        Tth111I     XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   65 enzymes) #
###################################

AclI         1688  AACGTT               AA^CGTT
AhdI          565  GACTATCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         712  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI      1560  AGCGCT               AGC^GCT
ApaI         1213  GGGCCC               GGGCC^C
Asp700I       703  GAAAGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         565  GACTATCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI        1695  GAGCAC               G[AT]GC[AT]^C
AvaI          123  CTCGGG               C^[CT]CG[GA]G
BalI         1346  TGGCCA               TGG^CCA
BamHI        1071  GGATCC               G^GATCC
BanII        1213  GGGCCC               G[GA]GC[CT]^C
BbrPI        1350  CACGTG               CAC^GTG
BcgI          750  GCAGAAAACTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          509  TGATCA               T^GATCA
BcnI          285  CCGGG                CC^[GC]GG
BfaI         1469  CTAG                 C^TAG
BglI         1518  GCCACCAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         309  AGATCT               A^GATCT
BseRI          46  GAGGAG               GAGGAG(10/8)
BsiHKAI      1695  GAGCAC               G[AT]GC[AT]^C
BsmI         1413  GCATTC               GAATGC(1/-1)
BsoBI         123  CTCGGG               C^[CT]CG[GA]G
BspHI         162  TCATGA               T^CATGA
Cac8I         812  GCTGGC               GC[ACGT]^[ACGT]GC
CfoI         1561  GCGC                 GCG^C
DraI          916  TTTAAA               TTT^AAA
DraIII        788  CACAGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          113  CCACGG               C^C[GA][CT]GG
EaeI         1346  TGGCCA               [CT]^GGCC[GA]
Eam1105I      565  GACTATCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1560  AGCGCT               AGC^GCT
EcoNI          39  CCTCTACGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         707  GAATTC               G^AATTC
EcoRV        1813  GATATC               GAT^ATC
EcoT22I      1750  ATGCAT               ATGCA^T
FbaI          509  TGATCA               T^GATCA
HaeII        1560  AGCGCT               [GA]GCGC^[CT]
HapII         285  CCGG                 C^CGG
HhaI         1561  GCGC                 GCG^C
HinP1I       1561  GCGC                 G^CGC
HindIII       413  AAGCTT               A^AGCTT
HpaII         285  CCGG                 C^CGG
MaeI         1469  CTAG                 C^TAG
MfeI          436  CAATTG               C^AATTG
MluNI        1346  TGGCCA               TGG^CCA
MscI         1346  TGGCCA               TGG^CCA
MslI         1188  CACTCCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          285  CCGG                 C^CGG
MunI          436  CAATTG               C^AATTG
NciI          285  CCGGG                CC^[GC]GG
NsiI         1750  ATGCAT               ATGCA^T
PflMI        1062  CCATCCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        1350  CACGTG               CAC^GTG
PmlI         1350  CACGTG               CAC^GTG
PshAI        1130  GACTTCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     1688  AACGTT               AA^CGTT
RcaI          162  TCATGA               T^CATGA
ScaI         1540  AGTACT               AGT^ACT
SexAI        1944  ACCTGGT              A^CC[AT]GGT
SfcI         1218  CTACAG               C^T[GA][CT]AG
SmlI         1544  CTCAAG               C^T[CT][GA]AG
SspI         1623  AATATT               AAT^ATT
Van91I       1062  CCATCCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI          703  GAAAGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

BsaAI        1350  CACGTG               [CT]AC^GT[GA]
             1824  CACGTA              
BsrDI        1445  CATTGC               GCAATG(2/0)
             1843  GCAATG              
BstBI         145  TTCGAA               TT^CGAA
             1835  TTCGAA              
EarI          142  CTCTTC               CTCTTC(1/4)
              248  GAAGAG              
HphI         1810  GGTGA                GGTGA(8/7)
              101  TCACC               
Ksp632I       142  CTCTTC               CTCTTC(1/4)
              248  GAAGAG              
NspV          145  TTCGAA               TT^CGAA
             1835  TTCGAA              
PpuMI        1420  AGGACCC              [GA]G^G[AT]CC[CT]
             1731  GGGTCCC             
SfuI          145  TTCGAA               TT^CGAA
             1835  TTCGAA              


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AvaII       BbsI        BbvI        BmyI        
BpmI        BpuAI       BsaI        BsaJI       BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstYI       Cfr13I      
DdeI        DpnII       DraII       EcoO109I    EcoRII      
Fnu4HI      FokI        HaeIII      HinfI       ItaI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       PleI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII       
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