Restriction map for pF1KB7874
 (Linear) (Six-base) MAPSORT of: KIBB7874.seq  Check: 1031  from: 1  to: 1911



KIBB7874.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:24  ..

Acc65I G'GTAC_C

Cuts at:      0     337     415    1911 
   Size:        337      78    1496

  Fragments arranged by size: 

                1496     337      78
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     911    1911 
   Size:        911    1000
 
AflIII A'CryG_T

Cuts at:      0      72     267    1911 
   Size:         72     195    1644

  Fragments arranged by size: 

                1644     195      72
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1661    1700    1911 
   Size:       1661      39     211

  Fragments arranged by size: 

                1661     211      39
 
AlwNI CAG_nnn'CTG

Cuts at:      0     601    1171    1911 
   Size:        601     570     740

  Fragments arranged by size: 

                 740     601     570
 
ApaLI G'TGCA_C

Cuts at:      0    1033    1911 
   Size:       1033     878
 
BanI G'GyrC_C

Cuts at:      0     337     415    1739    1810    1911 
   Size:        337      78    1324      71     101

  Fragments arranged by size: 

                1324     337     101      78      71
 
BanII G_rGCy'C

Cuts at:      0    1331    1911 
   Size:       1331     580
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1273    1911 
   Size:       1273     638
 
BbvCI CC'TCA_GC

Cuts at:      0    1626    1661    1911 
   Size:       1626      35     250

  Fragments arranged by size: 

                1626     250      35
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1127    1911 
   Size:       1127     784
 
BglII A'GATC_T

Cuts at:      0     933    1280    1911 
   Size:        933     347     631

  Fragments arranged by size: 

                 933     631     347
 
Bme1580I G_kGCm'C

Cuts at:      0    1037    1742    1911 
   Size:       1037     705     169

  Fragments arranged by size: 

                1037     705     169
 
BmgBI CAC'GTC

Cuts at:      0     858    1911 
   Size:        858    1053
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     518    1911 
   Size:        518    1393
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      71    1911 
   Size:         71    1840
 
Bpu10I CC'TnA_GC

Cuts at:      0    1626    1661    1911 
   Size:       1626      35     250

  Fragments arranged by size: 

                1626     250      35
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     742    1517    1682    1911 
   Size:        742     775     165     229

  Fragments arranged by size: 

                 775     742     229     165
 
BsaAI yAC'GTr

Cuts at:      0    1409    1911 
   Size:       1409     502
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     346     376    1911 
   Size:        346      30    1535

  Fragments arranged by size: 

                1535     346      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      68    1351    1354    1614    1911 
   Size:         68    1283       3     260     297

  Fragments arranged by size: 

                1283     297     260      68       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     487    1056    1911 
   Size:        487     569     855

  Fragments arranged by size: 

                 855     569     487
 
BsiEI CG_ry'CG

Cuts at:      0    1002    1911 
   Size:       1002     909
 
BsiHKAI G_wGCw'C

Cuts at:      0    1037    1911 
   Size:       1037     874
 
BsmI GAATG_Cn'

Cuts at:      0    1711    1911 
   Size:       1711     200
 
Bsp1286I G_dGCh'C

Cuts at:      0    1037    1331    1742    1911 
   Size:       1037     294     411     169

  Fragments arranged by size: 

                1037     411     294     169
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     487     586    1911 
   Size:        487      99    1325

  Fragments arranged by size: 

                1325     487      99
 
BsrGI T'GTAC_A

Cuts at:      0    1162    1208    1911 
   Size:       1162      46     703

  Fragments arranged by size: 

                1162     703      46
 
BstBI TT'CG_AA

Cuts at:      0     989    1911 
   Size:        989     922
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1569    1911 
   Size:       1569     342
 
BstYI r'GATC_y

Cuts at:      0     933    1280    1911 
   Size:        933     347     631

  Fragments arranged by size: 

                 933     631     347
 
BtgI C'CryG_G

Cuts at:      0     104    1911 
   Size:        104    1807
 
BtsI GCAGTG_nn'

Cuts at:      0     446    1911 
   Size:        446    1465
 
EaeI y'GGCC_r

Cuts at:      0     222    1292    1911 
   Size:        222    1070     619

  Fragments arranged by size: 

                1070     619     222
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      71     911    1911 
   Size:         71     840    1000

  Fragments arranged by size: 

                1000     840      71
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      53    1911 
   Size:         53    1858
 
EcoO109I rG'GnC_Cy

Cuts at:      0     971    1397    1911 
   Size:        971     426     514

  Fragments arranged by size: 

                 971     514     426
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1002    1405    1911 
   Size:       1002     403     506

  Fragments arranged by size: 

                1002     506     403
 
HincII GTy'rAC

Cuts at:      0    1483    1524    1911 
   Size:       1483      41     387

  Fragments arranged by size: 

                1483     387      41
 
HindIII A'AGCT_T

Cuts at:      0     144    1911 
   Size:        144    1767
 
KpnI G_GTAC'C

Cuts at:      0     341     419    1911 
   Size:        341      78    1492

  Fragments arranged by size: 

                1492     341      78
 
MfeI C'AATT_G

Cuts at:      0     521    1688    1911 
   Size:        521    1167     223

  Fragments arranged by size: 

                1167     521     223
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     218    1883    1905    1911 
   Size:        218    1665      22       6

  Fragments arranged by size: 

                1665     218      22       6
 
MscI TGG'CCA

Cuts at:      0     224    1294    1911 
   Size:        224    1070     617

  Fragments arranged by size: 

                1070     617     224
 
MslI CAynn'nnrTG

Cuts at:      0     708     888    1567    1911 
   Size:        708     180     679     344

  Fragments arranged by size: 

                 708     679     344     180
 
MspA1I CmG'CkG

Cuts at:      0     405    1911 
   Size:        405    1506
 
NdeI CA'TA_TG

Cuts at:      0    1155    1911 
   Size:       1155     756
 
NspI r_CATG'y

Cuts at:      0      76     271     661     811    1522    1545    1911 
   Size:         76     195     390     150     711      23     366

  Fragments arranged by size: 

                 711     390     366     195     150      76      23
 
PciI A'CATG_T

Cuts at:      0      72     267    1911 
   Size:         72     195    1644

  Fragments arranged by size: 

                1644     195      72
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1087    1911 
   Size:       1087     824
 
PfoI T'CCnGG_A

Cuts at:      0      48    1911 
   Size:         48    1863
 
PpuMI rG'GwC_Cy

Cuts at:      0    1397    1911 
   Size:       1397     514
 
PsiI TTA'TAA

Cuts at:      0    1118    1911 
   Size:       1118     793
 
PvuII CAG'CTG

Cuts at:      0     405    1911 
   Size:        405    1506
 
SanDI GG'GwC_CC

Cuts at:      0    1397    1911 
   Size:       1397     514
 
SfcI C'TryA_G

Cuts at:      0     937    1911 
   Size:        937     974
 
SmlI C'TyrA_G

Cuts at:      0     757    1532    1697    1911 
   Size:        757     775     165     214

  Fragments arranged by size: 

                 775     757     214     165
 
SnaBI TAC'GTA

Cuts at:      0    1409    1911 
   Size:       1409     502
 
SphI G_CATG'C

Cuts at:      0     811    1911 
   Size:        811    1100
 
SspI AAT'ATT

Cuts at:      0    1198    1911 
   Size:       1198     713
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     992    1163    1828    1911 
   Size:        992     171     665      83

  Fragments arranged by size: 

                 992     665     171      83
 
TatI w'GTAC_w

Cuts at:      0     321     907    1162    1208    1911 
   Size:        321     586     255      46     703

  Fragments arranged by size: 

                 703     586     321     255      46
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     346     378    1219    1251    1911 
   Size:        346      32     841      32     660

  Fragments arranged by size: 

                 841     660     346      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1478    1911 
   Size:       1478     433
 
XbaI T'CTAG_A

Cuts at:      0     303    1873    1911 
   Size:        303    1570      38

  Fragments arranged by size: 

                1570     303      38
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     543    1911 
   Size:        543    1368
 
XmnI GAAnn'nnTTC

Cuts at:      0    1385    1911 
   Size:       1385     526
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII     AlfI    AlwNI    ApaLI     BanI    BanII
     BbsI    BbvCI    BciVI    BglII Bme1580I    BmgBI     BmrI     BpmI
   Bpu10I    BpuEI    BsaAI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspMI    BsrGI    BstBI    BstXI    BstYI     BtgI
     BtsI     EaeI  Eco57MI    EcoNI EcoO109I     FalI   HincII  HindIII
     KpnI     MfeI     MmeI     MscI     MslI   MspA1I     NdeI     NspI
     PciI    PflMI     PfoI    PpuMI     PsiI    PvuII    SanDI     SfcI
     SmlI    SnaBI     SphI     SspI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AloI     ApaI     ApoI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BbeI     BcgI     BclI    BfrBI     BglI
     BlpI     BmtI     BplI     BsaI    BsaBI    BsaHI    BsaWI    BseYI
    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI   BssHII
    BssSI   BstAPI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I     HpaI     KasI
     MluI     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PmeI     PmlI     PpiI    PshAI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SpeI     SrfI     StuI     StyI     SwaI     XhoI     XmaI
     ZraI
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