Restriction map for pF1KB7656
##################################
# NO CUT ENZYMES (  143 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AviII       AvrII       
BamHI       BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BcnI        BfrI        
BlnI        BlpI        BmyI        Bpu1102I    BpuAI       
BsaBI       BsaHI       BsaWI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmI        
Bsp1286I    BspDI       BspEI       BspLU11I    BsrBI       
BsrFI       BssHII      Bst1107I    BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        FseI        FspI        HapII       
Hin1I       HincII      HindII      HpaI        HpaII       
KasI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MslI        MspA1I      MspI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RsrII       
SacI        SacII       SalI        SbfI        SexAI       
SfoI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspI        SwaI        Tth111I     
XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AlwI          111  GGATC                GGATC(4/5)
AlwNI         547  CAGCCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaII         725  GGACC                G^G[AT]CC
BanI         1151  GGCACC               G^G[CT][GA]CC
BclI          464  TGATCA               T^GATCA
BglII         102  AGATCT               A^GATCT
BsaAI         151  TACGTG               [CT]AC^GT[GA]
BsaI          602  GGTCTC               GGTCTC(1/5)
BseRI        1047  GAGGAG               GAGGAG(10/8)
BsmFI         724  GGGAC                GGGAC(10/14)
Bsp1407I      741  TGTACA               T^GTACA
BspMI         966  GCAGGT               ACCTGC(4/8)
BsrDI         314  CATTGC               GCAATG(2/0)
BsrGI         741  TGTACA               T^GTACA
BssSI         730  CTCGTG               CACGAG(-5/-1)
BstAPI        309  GCAACCATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         254  TTCGAA               TT^CGAA
BstYI         102  AGATCT               [GA]^GATC[CT]
CfoI          363  GCGC                 GCG^C
Cfr13I        725  GGACC                G^G[ACGT]CC
DraII         724  GGGACCC              [GA]G^G[ACGT]CC[CT]
DraIII        745  CACGATGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I      724  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoT14I       502  CCTTGG               C^C[AT][AT]GG
FbaI          464  TGATCA               T^GATCA
HaeII         362  AGCGCC               [GA]GCGC^[CT]
HgaI          801  GACGC                GACGC(5/10)
HhaI          363  GCGC                 GCG^C
HinP1I        363  GCGC                 G^CGC
HindIII       434  AAGCTT               A^AGCTT
MflI          102  AGATCT               [GA]^GATC[CT]
NdeI          164  CATATG               CA^TATG
NspV          254  TTCGAA               TT^CGAA
PaeR7I        916  CTCGAG               C^TCGAG
PflMI         663  CCACCACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         724  GGGACCC              [GA]G^G[AT]CC[CT]
Sau96I        725  GGACC                G^G[ACGT]CC
ScaI          566  AGTACT               AGT^ACT
SfcI          949  CTACAG               C^T[GA][CT]AG
SfiI         1168  GGCCAGCATGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI          254  TTCGAA               TT^CGAA
SspBI         741  TGTACA               T^GTACA
StuI          406  AGGCCT               AGG^CCT
StyI          502  CCTTGG               C^C[AT][AT]GG
Tsp45I        154  GTGAC                ^GT[GC]AC
Van91I        663  CCACCACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          916  CTCGAG               C^TCGAG
XhoII         102  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AciI          855  CCGC                 CCGC(-3/-1)
              393  GCGG                
Asp700I       285  GAATTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              420  GAAGAAATTC          
AvaI          916  CTCGAG               C^[CT]CG[GA]G
             1138  CTCGGG              
BalI         1167  TGGCCA               TGG^CCA
             1176  TGGCCA              
BfaI          223  CTAG                 C^TAG
              649  CTAG                
BglI          408  GCCTTCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1169  GCCAGCATGGC         
BpmI          441  CTGGAG               CTGGAG(16/14)
              766  CTGGAG              
BsaJI         502  CCTTGG               C^C[ACGT][ACGT]GG
             1137  CCTCGG              
BsmAI         247  GAGAC                GTCTC(1/5)
              603  GTCTC               
BsoBI         916  CTCGAG               C^[CT]CG[GA]G
             1138  CTCGGG              
BspHI         956  TCATGA               T^CATGA
             1025  TCATGA              
DraI           46  TTTAAA               TTT^AAA
             1125  TTTAAA              
EaeI         1167  TGGCCA               [CT]^GGCC[GA]
             1176  TGGCCA              
MaeI          223  CTAG                 C^TAG
              649  CTAG                
MaeII          43  ACGT                 A^CGT
              152  ACGT                
MluNI        1167  TGGCCA               TGG^CCA
             1176  TGGCCA              
MscI         1167  TGGCCA               TGG^CCA
             1176  TGGCCA              
RcaI          956  TCATGA               T^CATGA
             1025  TCATGA              
SapI           20  GAAGAGC              GCTCTTC(1/4)
             1029  GAAGAGC             
SfaNI        1005  GATGC                GCATC(5/9)
             1034  GCATC               
SmlI          106  CTTGAG               C^T[CT][GA]AG
              916  CTCGAG              
TaqI          255  TCGA                 T^CGA
              917  TCGA                
TfiI           71  GATTC                G^A[AT]TC
              396  GAATC               
TthHB8I       255  TCGA                 T^CGA
              917  TCGA                
XcmI           85  CCAACAATAAAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              559  CCATCCCAGTACTGG     
XmnI          285  GAATTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              420  GAAGAAATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   41 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        BbvI        
BsiYI       BslI        BsrI        BssKI       BstF5I      
BstNI       Cac8I       DdeI        DpnII       EarI        
EcoRII      Fnu4HI      FokI        HaeIII      HinfI       
HphI        ItaI        Ksp632I     MaeIII      MboI        
MboII       MnlI        MseI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       PleI        RsaI        
Sau3AI      ScrFI       Tru9I       TseI        Tsp509I     
TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com