Restriction map for pF1KB7656
 (Linear) (Six-base) MAPSORT of: KIBB7656.seq  Check: 1980  from: 1  to: 1182



KIBB7656.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:24  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     439    1182 
   Size:        439     743
 
AlwNI CAG_nnn'CTG

Cuts at:      0     552    1182 
   Size:        552     630
 
ApoI r'AATT_y

Cuts at:      0     205     285     424    1182 
   Size:        205      80     139     758

  Fragments arranged by size: 

                 758     205     139      80
 
AvaI C'yCGr_G

Cuts at:      0     916    1138    1182 
   Size:        916     222      44

  Fragments arranged by size: 

                 916     222      44
 
BanI G'GyrC_C

Cuts at:      0    1151    1182 
   Size:       1151      31
 
BclI T'GATC_A

Cuts at:      0     464    1182 
   Size:        464     718
 
BglI GCCn_nnn'nGGC

Cuts at:      0     414    1175    1182 
   Size:        414     761       7

  Fragments arranged by size: 

                 761     414       7
 
BglII A'GATC_T

Cuts at:      0     102    1182 
   Size:        102    1080
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     558     993    1182 
   Size:        558     435     189

  Fragments arranged by size: 

                 558     435     189
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     462     787    1182 
   Size:        462     325     395

  Fragments arranged by size: 

                 462     395     325
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127    1182 
   Size:        127    1055
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     608    1182 
   Size:        608     574
 
BsaAI yAC'GTr

Cuts at:      0     153    1182 
   Size:        153    1029
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1062    1182 
   Size:       1062     120
 
BspHI T'CATG_A

Cuts at:      0     956    1025    1182 
   Size:        956      69     157

  Fragments arranged by size: 

                 956     157      69
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     957    1182 
   Size:        957     225
 
BsrDI GCAATG_nn'

Cuts at:      0     313    1182 
   Size:        313     869
 
BsrGI T'GTAC_A

Cuts at:      0     741    1182 
   Size:        741     441
 
BssSI C'ACGA_G

Cuts at:      0     730    1182 
   Size:        730     452
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     315    1182 
   Size:        315     867
 
BstBI TT'CG_AA

Cuts at:      0     255    1182 
   Size:        255     927
 
BstYI r'GATC_y

Cuts at:      0     102    1182 
   Size:        102    1080
 
BtsI GCAGTG_nn'

Cuts at:      0      11     726    1182 
   Size:         11     715     456

  Fragments arranged by size: 

                 715     456      11
 
DraI TTT'AAA

Cuts at:      0      48    1127    1182 
   Size:         48    1079      55

  Fragments arranged by size: 

                1079      55      48
 
DraIII CAC_nnn'GTG

Cuts at:      0     750    1182 
   Size:        750     432
 
EaeI y'GGCC_r

Cuts at:      0    1167    1176    1182 
   Size:       1167       9       6

  Fragments arranged by size: 

                1167       9       6
 
EarI CTCTTCn'nnn_

Cuts at:      0      15    1024    1064    1070    1076    1088    1182 
   Size:         15    1009      40       6       6      12      94

  Fragments arranged by size: 

                1009      94      40      15      12       6       6
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     408    1182 
   Size:        408     774
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     439     462     787    1182 
   Size:        439      23     325     395

  Fragments arranged by size: 

                 439     395     325      23
 
EcoO109I rG'GnC_Cy

Cuts at:      0     725    1182 
   Size:        725     457
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     369    1182 
   Size:        369     813
 
HaeII r_GCGC'y

Cuts at:      0     366    1182 
   Size:        366     816
 
HindIII A'AGCT_T

Cuts at:      0     434    1182 
   Size:        434     748
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      44     109    1182 
   Size:         44      65    1073

  Fragments arranged by size: 

                1073      65      44
 
MscI TGG'CCA

Cuts at:      0    1169    1178    1182 
   Size:       1169       9       4

  Fragments arranged by size: 

                1169       9       4
 
NdeI CA'TA_TG

Cuts at:      0     165    1182 
   Size:        165    1017
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     669    1182 
   Size:        669     513
 
PfoI T'CCnGG_A

Cuts at:      0     428    1037    1182 
   Size:        428     609     145

  Fragments arranged by size: 

                 609     428     145
 
PpuMI rG'GwC_Cy

Cuts at:      0     725    1182 
   Size:        725     457
 
PspXI vC'TCGA_Gb

Cuts at:      0     916    1182 
   Size:        916     266
 
SanDI GG'GwC_CC

Cuts at:      0     725    1182 
   Size:        725     457
 
SapI GCTCTTCn'nnn_

Cuts at:      0      15    1024    1182 
   Size:         15    1009     158

  Fragments arranged by size: 

                1009     158      15
 
ScaI AGT'ACT

Cuts at:      0     568    1182 
   Size:        568     614
 
SfcI C'TryA_G

Cuts at:      0     949    1182 
   Size:        949     233
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1175    1182 
   Size:       1175       7
 
SmlI C'TyrA_G

Cuts at:      0     106     916    1182 
   Size:        106     810     266

  Fragments arranged by size: 

                 810     266     106
 
StuI AGG'CCT

Cuts at:      0     408    1182 
   Size:        408     774
 
StyI C'CwwG_G

Cuts at:      0     502    1182 
   Size:        502     680
 
TatI w'GTAC_w

Cuts at:      0     566     741    1182 
   Size:        566     175     441

  Fragments arranged by size: 

                 566     441     175
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      92     566    1182 
   Size:         92     474     616

  Fragments arranged by size: 

                 616     474      92
 
XhoI C'TCGA_G

Cuts at:      0     916    1182 
   Size:        916     266
 
XmnI GAAnn'nnTTC

Cuts at:      0     289     424    1182 
   Size:        289     135     758

  Fragments arranged by size: 

                 758     289     135
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI     AvaI     BanI     BclI     BglI    BglII
     BmrI     BpmI    BpuEI     BsaI    BsaAI    BseRI    BspHI    BspMI
    BsrDI    BsrGI    BssSI   BstAPI    BstBI    BstYI     BtsI     DraI
   DraIII     EaeI     EarI     EciI  Eco57MI EcoO109I     FalI    HaeII
  HindIII     MmeI     MscI     NdeI    PflMI     PfoI    PpuMI    PspXI
    SanDI     SapI     ScaI     SfcI     SfiI     SmlI     StuI     StyI
     TatI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI    BfrBI     BlpI Bme1580I    BmgBI     BmtI
     BplI   Bpu10I    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BsrBI
    BsrFI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DrdI     EagI  EcoICRI    EcoNI    EcoRI    EcoRV
     FseI     FspI    FspAI    Hin4I   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI     SbfI    SexAI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI    TaqII     TstI  Tth111I     XbaI     XmaI     ZraI
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