Restriction map for pF1KB7643
##################################
# NO CUT ENZYMES (  150 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
AlwI        AlwNI       AocI        Aor51HI     ApaI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AviII       AvrII       BamHI       BanI        
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsiWI       BspDI       
BspEI       BspHI       BspLU11I    BsrFI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DdeI        DpnII       DraI        DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRV       EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HindIII     HpaI        HphI        KasI        
KpnI        KspI        MboI        MflI        MluI        
MroI        MseI        MspA1I      MvnI        NaeI        
NarI        NcoI        NdeI        NdeII       NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PflFI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        Sau3AI      SbfI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspI        StuI        SwaI        
Tru9I       Tth111I     XbaI        XcmI        XhoII       


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AcsI          337  GAATTC               [GA]^AATT[CT]
AflIII        218  ACGTGT               A^C[GA][CT]GT
Alw44I        715  GTGCAC               G^TGCAC
ApaLI         715  GTGCAC               G^TGCAC
ApoI          337  GAATTC               [GA]^AATT[CT]
Asp700I       507  GAAGTACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         715  GTGCAC               G[AT]GC[AT]^C
BalI          816  TGGCCA               TGG^CCA
BanII         416  GAGCCC               G[GA]GC[CT]^C
BfaI          832  CTAG                 C^TAG
BglI          259  GCCTTCTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI           55  CTCCAG               CTGGAG(16/14)
BsaAI         217  TACGTG               [CT]AC^GT[GA]
BsaBI           3  GATGCTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI         411  CGACCG               CG[GA][CT]^CG
BsiHKAI       715  GTGCAC               G[AT]GC[AT]^C
BsmBI         736  GAGACG               CGTCTC(1/5)
BsmI          446  GCATTC               GAATGC(1/-1)
BsrBI          37  GAGCGG               CCGCTC(-3/-3)
BsrDI         586  CATTGC               GCAATG(2/0)
BsrI          885  CCAGT                ACTGG(1/-1)
BstF5I        794  GGATG                GGATG(2/0)
EaeI          816  TGGCCA               [CT]^GGCC[GA]
EcoRI         337  GAATTC               G^AATTC
EcoT14I       864  CCAAGG               C^C[AT][AT]GG
FokI          794  GGATG                GGATG(9/13)
HincII        478  GTCAAC               GT[CT]^[GA]AC
HindII        478  GTCAAC               GT[CT]^[GA]AC
MaeI          832  CTAG                 C^TAG
MaeII         218  ACGT                 A^CGT
MamI            3  GATGCTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          751  CAATTG               C^AATTG
MluNI         816  TGGCCA               TGG^CCA
MscI          816  TGGCCA               TGG^CCA
MunI          751  CAATTG               C^AATTG
PaeR7I        209  CTCGAG               C^TCGAG
PflMI         885  CCAGTATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         706  GGGACCC              [GA]G^G[AT]CC[CT]
SapI          675  GCTCTTC              GCTCTTC(1/4)
ScaI          509  AGTACT               AGT^ACT
SexAI         151  ACCTGGT              A^CC[AT]GGT
SfiI          258  GGCCTTCTTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI          304  CCCGGG               CCC^GGG
SmlI          209  CTCGAG               C^T[CT][GA]AG
StyI          864  CCAAGG               C^C[AT][AT]GG
Tsp45I        119  GTGAC                ^GT[GC]AC
Van91I        885  CCAGTATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          209  CTCGAG               C^TCGAG
XmaCI         304  CCCGGG               C^CCGGG
XmaI          304  CCCGGG               C^CCGGG
XmnI          507  GAAGTACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AluI          537  AGCT                 AG^CT
              616  AGCT                
AvaI          209  CTCGAG               C^[CT]CG[GA]G
              304  CCCGGG              
BsgI          726  CTGCAC               GTGCAG(16/14)
              612  GTGCAG              
BsmAI         212  GAGAC                GTCTC(1/5)
              736  GAGAC               
BsoBI         209  CTCGAG               C^[CT]CG[GA]G
              304  CCCGGG              
Bsp1407I      377  TGTACA               T^GTACA
              530  TGTACA              
BspMI         251  ACCTGC               ACCTGC(4/8)
              369  GCAGGT              
BsrGI         377  TGTACA               T^GTACA
              530  TGTACA              
DraII         668  AGGGCCT              [GA]G^G[ACGT]CC[CT]
              706  GGGACCC             
EarI          676  CTCTTC               CTCTTC(1/4)
              763  GAAGAG              
EcoO109I      668  AGGGCCT              [GA]G^G[ACGT]CC[CT]
              706  GGGACCC             
HapII         285  CCGG                 C^CGG
              305  CCGG                
HpaII         285  CCGG                 C^CGG
              305  CCGG                
Ksp632I       676  CTCTTC               CTCTTC(1/4)
              763  GAAGAG              
MaeIII        119  GTGAC                ^GT[ACGT]AC
              148  GTTAC               
MspI          285  CCGG                 C^CGG
              305  CCGG                
PstI          396  CTGCAG               CTGCA^G
              426  CTGCAG              
SfcI          396  CTGCAG               C^T[GA][CT]AG
              426  CTGCAG              
SspBI         377  TGTACA               T^GTACA
              530  TGTACA              
TaqI          210  TCGA                 T^CGA
              344  TCGA                
TthHB8I       210  TCGA                 T^CGA
              344  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   36 enzymes) #
#########################################

AciI        AfaI        AvaII       BbvI        BcnI        
BmyI        BsaJI       BsiYI       BslI        BsmFI       
Bsp1286I    BssKI       BstNI       Cac8I       Cfr13I      
EcoRII      Fnu4HI      HaeIII      HinfI       ItaI        
MboII       MnlI        MslI        MvaI        MwoI        
NciI        NlaIII      NlaIV       RsaI        Sau96I      
ScrFI       SfaNI       TfiI        TseI        Tsp509I     
TspRI       
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