Restriction map for pF1KB7643
 (Linear) (Six-base) MAPSORT of: KIBB7643.seq  Check: 3479  from: 1  to: 897



KIBB7643.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:24  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     360     897 
   Size:        360     537
 
AflIII A'CryG_T

Cuts at:      0     218     897 
   Size:        218     679
 
AleI CACnn'nnGTG

Cuts at:      0     428     637     694     897 
   Size:        428     209      57     203

  Fragments arranged by size: 

                 428     209     203      57
 
ApaLI G'TGCA_C

Cuts at:      0     715     897 
   Size:        715     182
 
ApoI r'AATT_y

Cuts at:      0     337     897 
   Size:        337     560
 
AvaI C'yCGr_G

Cuts at:      0     209     304     897 
   Size:        209      95     593

  Fragments arranged by size: 

                 593     209      95
 
BanII G_rGCy'C

Cuts at:      0     420     897 
   Size:        420     477
 
BglI GCCn_nnn'nGGC

Cuts at:      0     265     897 
   Size:        265     632
 
Bme1580I G_kGCm'C

Cuts at:      0     317     388     719     897 
   Size:        317      71     331     178

  Fragments arranged by size: 

                 331     317     178      71
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     897 
   Size:         40     857
 
BsaAI yAC'GTr

Cuts at:      0     219     897 
   Size:        219     678
 
BsaBI GATnn'nnATC

Cuts at:      0       7     897 
   Size:          7     890
 
BseYI C'CCAG_C

Cuts at:      0     836     897 
   Size:        836      61
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     633     711     897 
   Size:        633      78     186

  Fragments arranged by size: 

                 633     186      78
 
BsiEI CG_ry'CG

Cuts at:      0     414     897 
   Size:        414     483
 
BsiHKAI G_wGCw'C

Cuts at:      0     719     897 
   Size:        719     178
 
BsmI GAATG_Cn'

Cuts at:      0     446     897 
   Size:        446     451
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     730     897 
   Size:        730     167
 
Bsp1286I G_dGCh'C

Cuts at:      0     317     388     420     719     897 
   Size:        317      71      32     299     178

  Fragments arranged by size: 

                 317     299     178      71      32
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     260     360     897 
   Size:        260     100     537

  Fragments arranged by size: 

                 537     260     100
 
BsrBI CCG'CTC

Cuts at:      0      39     897 
   Size:         39     858
 
BsrDI GCAATG_nn'

Cuts at:      0     585     897 
   Size:        585     312
 
BsrGI T'GTAC_A

Cuts at:      0     377     530     897 
   Size:        377     153     367

  Fragments arranged by size: 

                 377     367     153
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     117     897 
   Size:        117     780
 
BtsI GCAGTG_nn'

Cuts at:      0     279     317     423     435     897 
   Size:        279      38     106      12     462

  Fragments arranged by size: 

                 462     279     106      38      12
 
EaeI y'GGCC_r

Cuts at:      0     816     897 
   Size:        816      81
 
EarI CTCTTCn'nnn_

Cuts at:      0     682     758     897 
   Size:        682      76     139

  Fragments arranged by size: 

                 682     139      76
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     897 
   Size:         40     857
 
EcoO109I rG'GnC_Cy

Cuts at:      0     669     707     897 
   Size:        669      38     190

  Fragments arranged by size: 

                 669     190      38
 
EcoRI G'AATT_C

Cuts at:      0     337     897 
   Size:        337     560
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     192     224     897 
   Size:        192      32     673

  Fragments arranged by size: 

                 673     192      32
 
HincII GTy'rAC

Cuts at:      0     480     897 
   Size:        480     417
 
MfeI C'AATT_G

Cuts at:      0     751     897 
   Size:        751     146
 
MscI TGG'CCA

Cuts at:      0     818     897 
   Size:        818      79
 
MslI CAynn'nnrTG

Cuts at:      0     428     565     637     694     897 
   Size:        428     137      72      57     203

  Fragments arranged by size: 

                 428     203     137      72      57
 
PasI CC'CwG_GG

Cuts at:      0     473     897 
   Size:        473     424
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     891     897 
   Size:        891       6
 
PpuMI rG'GwC_Cy

Cuts at:      0     707     897 
   Size:        707     190
 
PstI C_TGCA'G

Cuts at:      0     400     430     897 
   Size:        400      30     467

  Fragments arranged by size: 

                 467     400      30
 
SanDI GG'GwC_CC

Cuts at:      0     707     897 
   Size:        707     190
 
SapI GCTCTTCn'nnn_

Cuts at:      0     682     897 
   Size:        682     215
 
ScaI AGT'ACT

Cuts at:      0     511     897 
   Size:        511     386
 
SexAI A'CCwGG_T

Cuts at:      0     151     897 
   Size:        151     746
 
SfcI C'TryA_G

Cuts at:      0     396     426     897 
   Size:        396      30     471

  Fragments arranged by size: 

                 471     396      30
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     265     897 
   Size:        265     632
 
SmaI CCC'GGG

Cuts at:      0     306     897 
   Size:        306     591
 
SmlI C'TyrA_G

Cuts at:      0     209     897 
   Size:        209     688
 
StyI C'CwwG_G

Cuts at:      0     864     897 
   Size:        864      33
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      31     428     645     897 
   Size:         31     397     217     252

  Fragments arranged by size: 

                 397     252     217      31
 
TatI w'GTAC_w

Cuts at:      0     377     509     530     897 
   Size:        377     132      21     367

  Fragments arranged by size: 

                 377     367     132      21
 
XhoI C'TCGA_G

Cuts at:      0     209     897 
   Size:        209     688
 
XmaI C'CCGG_G

Cuts at:      0     304     897 
   Size:        304     593
 
XmnI GAAnn'nnTTC

Cuts at:      0     511     897 
   Size:        511     386
 

 Enzymes that do cut:

     AarI   AflIII     AleI    ApaLI     ApoI     AvaI    BanII     BglI
 Bme1580I     BpmI    BsaAI    BsaBI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrGI    BtgZI
     BtsI     EaeI     EarI  Eco57MI EcoO109I    EcoRI    Hin4I   HincII
     MfeI     MscI     MslI     PasI    PflMI    PpuMI     PstI    SanDI
     SapI     ScaI    SexAI     SfcI     SfiI     SmaI     SmlI     StyI
    TaqII     TatI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BsaXI    BseRI
    BsiWI    BspEI    BspHI    BsrFI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI
     KpnI     MluI     MmeI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PciI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI     SbfI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TstI  Tth111I     XbaI     XcmI     ZraI
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