Restriction map for pF1KB7841
##################################
# NO CUT ENZYMES (  129 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AflII       AflIII      AhdI        AocI        
Aor51HI     ApaI        AscI        Asp718I     AspEI       
AviII       AvrII       BalI        BanII       BbrPI       
BbsI        BbvCI       BcgI        BciVI       BfrI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaI        BseAI       
BseRI       BsiEI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspLU11I    BspMI       
BsrBI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DrdI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRV       EcoT22I     FauI        FseI        
FspI        HindIII     HpaI        KpnI        KspI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MspA1I      MunI        MvnI        NcoI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PmaCI       PmeI        PmlI        
PshAI       Psp1406I    PstI        PvuI        PvuII       
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfuI        SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AciI          505  GCGG                 CCGC(-3/-1)
AfaI          556  GTAC                 GT^AC
AgeI         1396  ACCGGT               A^CCGGT
Alw44I         46  GTGCAC               G^TGCAC
AlwNI          82  CAGGAACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI          46  GTGCAC               G^TGCAC
AseI         1369  ATTAAT               AT^TAAT
AsnI         1369  ATTAAT               AT^TAAT
Asp700I       976  GAACAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1385  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI           69  CTCGGG               C^[CT]CG[GA]G
BamHI         620  GGATCC               G^GATCC
BbeI           91  GGCGCC               GGCGC^C
BclI         1419  TGATCA               T^GATCA
BfaI          683  CTAG                 C^TAG
BglI           65  GCCTCTCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI        1396  ACCGGT               [AT]^CCGG[AT]
BsmAI         428  GAGAC                GTCTC(1/5)
BsoBI          69  CTCGGG               C^[CT]CG[GA]G
BspHI         768  TCATGA               T^CATGA
BstYI         620  GGATCC               [GA]^GATC[CT]
DraIII        813  CACAGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoRI        1133  GAATTC               G^AATTC
EcoT14I       261  CCAAGG               C^C[AT][AT]GG
FbaI         1419  TGATCA               T^GATCA
HgaI           58  GACGC                GACGC(5/10)
HincII        628  GTTGAC               GT[CT]^[GA]AC
HindII        628  GTTGAC               GT[CT]^[GA]AC
KasI           91  GGCGCC               G^GCGCC
MaeI          683  CTAG                 C^TAG
MaeII         214  ACGT                 A^CGT
MaeIII        216  GTCAC                ^GT[ACGT]AC
MflI          620  GGATCC               [GA]^GATC[CT]
NaeI           94  GCCGGC               GCC^GGC
NarI           91  GGCGCC               GG^CGCC
NdeI          272  CATATG               CA^TATG
NgoMIV         94  GCCGGC               G^CCGGC
NheI          682  GCTAGC               G^CTAGC
PflFI        1385  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         160  CCATTGGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        1396  ACCGGT               A^CCGGT
PleI           34  GAGTC                GAGTC(4/5)
PshBI        1369  ATTAAT               AT^TAAT
RcaI          768  TCATGA               T^CATGA
RsaI          556  GTAC                 GT^AC
SfoI           91  GGCGCC               GGC^GCC
SgrAI          93  CGCCGGCG             C[GA]^CCGG[CT]G
SmlI           13  CTCAAG               C^T[CT][GA]AG
StyI          261  CCAAGG               C^C[AT][AT]GG
Tsp45I        216  GTCAC                ^GT[GC]AC
Tth111I      1385  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        160  CCATTGGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          152  CCAGCCTTCCATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         620  GGATCC               [GA]^GATC[CT]
XmnI          976  GAACAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AcyI           58  GACGCC               G[GA]^CG[CT]C
               91  GGCGCC              
AvaII         522  GGACC                G^G[AT]CC
             1390  GGTCC               
BcnI           38  CCGGG                CC^[GC]GG
             1197  CCGGG               
BsaHI          58  GACGCC               G[GA]^CG[CT]C
               91  GGCGCC              
BsgI          716  GTGCAG               GTGCAG(16/14)
             1121  GTGCAG              
BsiYI         156  CCTTCCATTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              160  CCATTGGATGG         
BslI          156  CCTTCCATTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              160  CCATTGGATGG         
BsrDI         125  CATTGC               GCAATG(2/0)
             1014  GCAATG              
BsrFI          94  GCCGGC               [GA]^CCGG[CT]
             1396  ACCGGT              
BsrI           87  ACTGG                ACTGG(1/-1)
              495  CCAGT               
BstAPI       1014  GCAATGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1243  GCAAGCAGTGC         
Cfr10I         94  GCCGGC               [GA]^CCGG[CT]
             1396  ACCGGT              
DraII         521  GGGACCC              [GA]G^G[ACGT]CC[CT]
             1389  GGGTCCC             
DsaI          583  CCACGG               C^C[GA][CT]GG
              826  CCGTGG              
EarI         1038  CTCTTC               CTCTTC(1/4)
              756  GAAGAG              
EcoO109I      521  GGGACCC              [GA]G^G[ACGT]CC[CT]
             1389  GGGTCCC             
HaeIII          3  GGCC                 GG^CC
              830  GGCC                
Hin1I          58  GACGCC               G[GA]^CG[CT]C
               91  GGCGCC              
Ksp632I      1038  CTCTTC               CTCTTC(1/4)
              756  GAAGAG              
MslI          592  CACGGAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1010  CACAGCAATG          
NciI           38  CCGGG                CC^[GC]GG
             1197  CCGGG               
PpuMI         521  GGGACCC              [GA]G^G[AT]CC[CT]
             1389  GGGTCCC             


#########################################
# 3 OR MORE CUT ENZYMES (   52 enzymes) #
#########################################

AcsI        AluI        AlwI        ApoI        AspHI       
BanI        BbvI        BmyI        BsaJI       BsiHKAI     
BsmFI       Bsp1286I    BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
EcoRII      Fnu4HI      FokI        HaeII       HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MboI        MboII       MnlI        MseI        
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       Sau3AI      Sau96I      ScrFI       SfaNI       
TaqI        TfiI        Tru9I       TseI        Tsp509I     
TspRI       TthHB8I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com