Restriction map for pF1KB7841
 (Linear) (Six-base) MAPSORT of: KIBB7841.seq  Check: 1081  from: 1  to: 1512



KIBB7841.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:24  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     974    1174    1512 
   Size:        974     200     338

  Fragments arranged by size: 

                 974     338     200
 
AgeI A'CCGG_T

Cuts at:      0    1396    1512 
   Size:       1396     116
 
AleI CACnn'nnGTG

Cuts at:      0     596    1512 
   Size:        596     916
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1234    1237    1256    1512 
   Size:       1234       3      19     256

  Fragments arranged by size: 

                1234     256      19       3
 
AlwNI CAG_nnn'CTG

Cuts at:      0      87    1512 
   Size:         87    1425
 
ApaLI G'TGCA_C

Cuts at:      0      46    1512 
   Size:         46    1466
 
ApoI r'AATT_y

Cuts at:      0     278     511     574     980    1112    1133    1434    1512 
   Size:        278     233      63     406     132      21     301      78

  Fragments arranged by size: 

                 406     301     278     233     132      78      63      21
            
 
AseI AT'TA_AT

Cuts at:      0    1370    1512 
   Size:       1370     142
 
AvaI C'yCGr_G

Cuts at:      0      69    1512 
   Size:         69    1443
 
BamHI G'GATC_C

Cuts at:      0     620    1512 
   Size:        620     892
 
BanI G'GyrC_C

Cuts at:      0      91    1072    1272    1512 
   Size:         91     981     200     240

  Fragments arranged by size: 

                 981     240     200      91
 
BbeI G_GCGC'C

Cuts at:      0      95    1512 
   Size:         95    1417
 
BclI T'GATC_A

Cuts at:      0    1419    1512 
   Size:       1419      93
 
BglI GCCn_nnn'nGGC

Cuts at:      0      71    1512 
   Size:         71    1441
 
Bme1580I G_kGCm'C

Cuts at:      0      50     614    1275    1512 
   Size:         50     564     661     237

  Fragments arranged by size: 

                 661     564     237      50
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      97    1512 
   Size:         97    1415
 
BmtI G_CTAG'C

Cuts at:      0     686    1512 
   Size:        686     826
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     782    1512 
   Size:        782     730
 
BsaHI Gr'CG_yC

Cuts at:      0      59      92    1512 
   Size:         59      33    1420

  Fragments arranged by size: 

                1420      59      33
 
BsaWI w'CCGG_w

Cuts at:      0    1396    1512 
   Size:       1396     116
 
BseYI C'CCAG_C

Cuts at:      0     525     870    1512 
   Size:        525     345     642

  Fragments arranged by size: 

                 642     525     345
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     737    1142    1512 
   Size:        737     405     370

  Fragments arranged by size: 

                 737     405     370
 
BsiHKAI G_wGCw'C

Cuts at:      0      14      50     182     904    1512 
   Size:         14      36     132     722     608

  Fragments arranged by size: 

                 722     608     132      36      14
 
Bsp1286I G_dGCh'C

Cuts at:      0      14      50     182     614     904    1275    1512 
   Size:         14      36     132     432     290     371     237

  Fragments arranged by size: 

                 432     371     290     237     132      36      14
 
BspHI T'CATG_A

Cuts at:      0     768    1512 
   Size:        768     744
 
BsrDI GCAATG_nn'

Cuts at:      0     124    1021    1512 
   Size:        124     897     491

  Fragments arranged by size: 

                 897     491     124
 
BsrFI r'CCGG_y

Cuts at:      0      94    1396    1512 
   Size:         94    1302     116

  Fragments arranged by size: 

                1302     116      94
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1020    1249    1512 
   Size:       1020     229     263

  Fragments arranged by size: 

                1020     263     229
 
BstYI r'GATC_y

Cuts at:      0     620    1512 
   Size:        620     892
 
BtgI C'CryG_G

Cuts at:      0     583     826    1512 
   Size:        583     243     686

  Fragments arranged by size: 

                 686     583     243
 
BtsI GCAGTG_nn'

Cuts at:      0     558     915    1254    1272    1512 
   Size:        558     357     339      18     240

  Fragments arranged by size: 

                 558     357     339     240      18
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     443     478    1512 
   Size:        443      35    1034

  Fragments arranged by size: 

                1034     443      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     818    1512 
   Size:        818     694
 
EarI CTCTTCn'nnn_

Cuts at:      0     751    1044    1512 
   Size:        751     293     468

  Fragments arranged by size: 

                 751     468     293
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     974    1174    1512 
   Size:        974     200     338

  Fragments arranged by size: 

                 974     338     200
 
EcoO109I rG'GnC_Cy

Cuts at:      0     522    1390    1512 
   Size:        522     868     122

  Fragments arranged by size: 

                 868     522     122
 
EcoRI G'AATT_C

Cuts at:      0    1133    1512 
   Size:       1133     379
 
HaeII r_GCGC'y

Cuts at:      0      95     101     877    1512 
   Size:         95       6     776     635

  Fragments arranged by size: 

                 776     635      95       6
 
HincII GTy'rAC

Cuts at:      0     630    1512 
   Size:        630     882
 
KasI G'GCGC_C

Cuts at:      0      91    1512 
   Size:         91    1421
 
MslI CAynn'nnrTG

Cuts at:      0     596    1014    1512 
   Size:        596     418     498

  Fragments arranged by size: 

                 596     498     418
 
NaeI GCC'GGC

Cuts at:      0      96    1512 
   Size:         96    1416
 
NarI GG'CG_CC

Cuts at:      0      92    1512 
   Size:         92    1420
 
NdeI CA'TA_TG

Cuts at:      0     273    1512 
   Size:        273    1239
 
NgoMIV G'CCGG_C

Cuts at:      0      94    1512 
   Size:         94    1418
 
NheI G'CTAG_C

Cuts at:      0     682    1512 
   Size:        682     830
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     166    1512 
   Size:        166    1346
 
PfoI T'CCnGG_A

Cuts at:      0    1001    1512 
   Size:       1001     511
 
PpuMI rG'GwC_Cy

Cuts at:      0     522    1390    1512 
   Size:        522     868     122

  Fragments arranged by size: 

                 868     522     122
 
SanDI GG'GwC_CC

Cuts at:      0     522    1390    1512 
   Size:        522     868     122

  Fragments arranged by size: 

                 868     522     122
 
SfoI GGC'GCC

Cuts at:      0      93    1512 
   Size:         93    1419
 
SgrAI Cr'CCGG_yG

Cuts at:      0      94    1512 
   Size:         94    1418
 
SmlI C'TyrA_G

Cuts at:      0      13    1512 
   Size:         13    1499
 
StyI C'CwwG_G

Cuts at:      0     261    1512 
   Size:        261    1251
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     197     295     755    1512 
   Size:        197      98     460     757

  Fragments arranged by size: 

                 757     460     197      98
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     291     323    1512 
   Size:        291      32    1189

  Fragments arranged by size: 

                1189     291      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1388    1512 
   Size:       1388     124
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     159    1512 
   Size:        159    1353
 
XmnI GAAnn'nnTTC

Cuts at:      0     980    1512 
   Size:        980     532
 

 Enzymes that do cut:

     AcuI     AgeI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
     AvaI    BamHI     BanI     BbeI     BclI     BglI Bme1580I     BmrI
     BmtI     BplI    BsaHI    BsaWI    BseYI     BsgI  BsiHKAI Bsp1286I
    BspHI    BsrDI    BsrFI   BstAPI    BstYI     BtgI     BtsI    CspCI
   DraIII     EarI  Eco57MI EcoO109I    EcoRI    HaeII   HincII     KasI
     MslI     NaeI     NarI     NdeI   NgoMIV     NheI    PflMI     PfoI
    PpuMI    SanDI     SfoI    SgrAI     SmlI     StyI    TaqII     TstI
  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AhdI     AloI     ApaI     AscI    AsiSI    AvrII     BaeI    BanII
     BbsI    BbvCI     BcgI    BciVI    BfrBI    BglII     BlpI    BmgBI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaXI    BseRI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrGI
   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI
     ClaI     DraI     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
    EcoRV     FalI     FseI     FspI    FspAI    Hin4I  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NcoI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SgfI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TatI     XbaI     XhoI     XmaI
     ZraI
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