Restriction map for pF1KB9522
##################################
# NO CUT ENZYMES (  101 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AflII       AgeI        AocI        Aor51HI     
AscI        AseI        AsnI        Asp718I     AspI        
AvrII       BbeI        BbrPI       BciVI       BclI        
BfrI        BglI        BglII       BlnI        BsaBI       
BseAI       BsiWI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BsrGI       BssHII      Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstZ17I     
Bsu36I      ClaI        CpoI        DraI        Eco47III    
Eco81I      EcoO65I     EcoRV       EcoT22I     FbaI        
FseI        HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MseI        MunI        MvnI        
NarI        NdeI        NotI        NruI        NsiI        
NspV        PacI        PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SbfI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        Tru9I       Tth111I     
XbaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AcsI          906  GAATTC               [GA]^AATT[CT]
AhdI          670  GACATGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI          393  GGGCCC               GGGCC^C
ApoI          906  GAATTC               [GA]^AATT[CT]
Asp700I        88  GAAGACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         670  GACATGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         161  TGCGCA               TGC^GCA
BalI         1739  TGGCCA               TGG^CCA
BamHI        1670  GGATCC               G^GATCC
BbvCI        1824  CCTCAGC              CCTCAGC(-5/-2)
BsaAI        1222  TACGTG               [CT]AC^GT[GA]
BsaWI         692  ACCGGA               [AT]^CCGG[AT]
BsiEI         500  CGGCCG               CG[GA][CT]^CG
BsmBI        1236  CGTCTC               CGTCTC(1/5)
BsrBI         503  CCGCTC               CCGCTC(-3/-3)
BstAPI       1764  GCAGATCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII        705  CACGGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          500  CGGCCG               C^GGCCG
Eam1105I      670  GACATGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI         500  CGGCCG               C^GGCCG
Eco52I        500  CGGCCG               C^GGCCG
EcoRI         906  GAATTC               G^AATTC
FspI          161  TGCGCA               TGC^GCA
MaeII        1223  ACGT                 A^CGT
MluNI        1739  TGGCCA               TGG^CCA
MscI         1739  TGGCCA               TGG^CCA
NaeI          730  GCCGGC               GCC^GGC
NgoMIV        730  GCCGGC               G^CCGGC
NheI         1746  GCTAGC               G^CTAGC
PaeR7I       1465  CTCGAG               C^TCGAG
PvuII         550  CAGCTG               CAG^CTG
SacI          532  GAGCTC               GAGCT^C
ScaI          791  AGTACT               AGT^ACT
StuI          584  AGGCCT               AGG^CCT
TfiI          659  GATTC                G^A[AT]TC
Tsp509I       907  AATT                 ^AATT
XcmI          911  CCAGCCAAGTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1465  CTCGAG               C^TCGAG
XmnI           88  GAAGACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AcyI         1186  GACGCC               G[GA]^CG[CT]C
             1645  GGCGTC              
AflIII        524  ACATGT               A^C[GA][CT]GT
             1803  ACATGT              
Alw44I       1192  GTGCAC               G^TGCAC
             1315  GTGCAC              
ApaLI        1192  GTGCAC               G^TGCAC
             1315  GTGCAC              
BcgI         1355  CGAGCTTCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              164  GCATCTCCATCG        
BfaI          350  CTAG                 C^TAG
             1747  CTAG                
BsaHI        1186  GACGCC               G[GA]^CG[CT]C
             1645  GGCGTC              
BsaI         1099  GAGACC               GGTCTC(1/5)
             1167  GGTCTC              
BspLU11I      524  ACATGT               A^CATGT
             1803  ACATGT              
BsrDI        1263  CATTGC               GCAATG(2/0)
             1032  GCAATG              
BsrFI         730  GCCGGC               [GA]^CCGG[CT]
             1374  GCCGGT              
BssSI        1353  CACGAG               CACGAG(-5/-1)
             1489  CTCGTG              
BstYI        1670  GGATCC               [GA]^GATC[CT]
             1766  AGATCC              
Cfr10I        730  GCCGGC               [GA]^CCGG[CT]
             1374  GCCGGT              
DrdI            3  GACTCCCCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1678  GACTCCCTGGTC        
FauI          399  CCCGC                CCCGC(4/6)
              610  CCCGC               
HaeII        1540  AGCGCC               [GA]GCGC^[CT]
             2014  AGCGCC              
Hin1I        1186  GACGCC               G[GA]^CG[CT]C
             1645  GGCGTC              
MaeI          350  CTAG                 C^TAG
             1747  CTAG                
MflI         1670  GGATCC               [GA]^GATC[CT]
             1766  AGATCC              
NcoI         1544  CCATGG               C^CATGG
             1754  CCATGG              
PpuMI         542  GGGACCT              [GA]G^G[AT]CC[CT]
              740  AGGACCT             
SapI         1998  GAAGAGC              GCTCTTC(1/4)
               48  GCTCTTC             
SmaI         1071  CCCGGG               CCC^GGG
             1976  CCCGGG              
XhoII        1670  GGATCC               [GA]^GATC[CT]
             1766  AGATCC              
XmaCI        1071  CCCGGG               C^CCGGG
             1976  CCCGGG              
XmaI         1071  CCCGGG               C^CCGGG
             1976  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   91 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AspHI       AvaI        AvaII       BanI        BanII       
BbsI        BbvI        BcnI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrI        
BssKI       BstF5I      BstNI       BstXI       Cac8I       
CelII       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoNI       
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeIII      MboI        MboII       MnlI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        TseI        Tsp45I      TspRI       TthHB8I     
Van91I      
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