Restriction map for pF1KB9522
 (Linear) (Six-base) MAPSORT of: KIBB9522.seq  Check: 5367  from: 1  to: 2061



KIBB9522.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:39  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1453    2061 
   Size:       1453     608
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     589     861    1698    2017    2061 
   Size:        589     272     837     319      44

  Fragments arranged by size: 

                 837     589     319     272      44
 
AflIII A'CryG_T

Cuts at:      0     524    1803    2061 
   Size:        524    1279     258

  Fragments arranged by size: 

                1279     524     258
 
AhdI GACnn_n'nnGTC

Cuts at:      0     675    2061 
   Size:        675    1386
 
AleI CACnn'nnGTG

Cuts at:      0    1756    2061 
   Size:       1756     305
 
AlwNI CAG_nnn'CTG

Cuts at:      0      15     567     633     744    1393    1770    2061 
   Size:         15     552      66     111     649     377     291

  Fragments arranged by size: 

                 649     552     377     291     111      66      15
 
ApaI G_GGCC'C

Cuts at:      0     397    2061 
   Size:        397    1664
 
ApaLI G'TGCA_C

Cuts at:      0    1192    1315    2061 
   Size:       1192     123     746

  Fragments arranged by size: 

                1192     746     123
 
ApoI r'AATT_y

Cuts at:      0     906    2061 
   Size:        906    1155
 
AvaI C'yCGr_G

Cuts at:      0    1071    1295    1465    1976    2034    2061 
   Size:       1071     224     170     511      58      27

  Fragments arranged by size: 

                1071     511     224     170      58      27
 
BamHI G'GATC_C

Cuts at:      0    1670    2061 
   Size:       1670     391
 
BanI G'GyrC_C

Cuts at:      0      64    1507    1879    2061 
   Size:         64    1443     372     182

  Fragments arranged by size: 

                1443     372     182      64
 
BanII G_rGCy'C

Cuts at:      0     397     436     536     983    2061 
   Size:        397      39     100     447    1078

  Fragments arranged by size: 

                1078     447     397     100      39
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      95    1058    1332    2061 
   Size:         95     963     274     729

  Fragments arranged by size: 

                 963     729     274      95
 
BbvCI CC'TCA_GC

Cuts at:      0    1825    2061 
   Size:       1825     236
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     153     187    1344    1378    2061 
   Size:        153      34    1157      34     683

  Fragments arranged by size: 

                1157     683     153      34      34
 
BlpI GC'TnA_GC

Cuts at:      0     556    1366    1537    2061 
   Size:        556     810     171     524

  Fragments arranged by size: 

                 810     556     524     171
 
Bme1580I G_kGCm'C

Cuts at:      0      69     397    1196    1319    2061 
   Size:         69     328     799     123     742

  Fragments arranged by size: 

                 799     742     328     123      69
 
BmrI ACTGGGnnnn_n'

Cuts at:      0       3     449    2061 
   Size:          3     446    1612

  Fragments arranged by size: 

                1612     446       3
 
BmtI G_CTAG'C

Cuts at:      0    1750    2061 
   Size:       1750     311
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     404    1310    2061 
   Size:        404     906     751

  Fragments arranged by size: 

                 906     751     404
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     532     655     703     721    1266    1622    2061 
   Size:        532     123      48      18     545     356     439

  Fragments arranged by size: 

                 545     532     439     356     123      48      18
 
Bpu10I CC'TnA_GC

Cuts at:      0     389    1207    1825    2061 
   Size:        389     818     618     236

  Fragments arranged by size: 

                 818     618     389     236
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     573    1516    2061 
   Size:        573     943     545

  Fragments arranged by size: 

                 943     573     545
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1093    1173    2061 
   Size:       1093      80     888

  Fragments arranged by size: 

                1093     888      80
 
BsaAI yAC'GTr

Cuts at:      0    1224    2061 
   Size:       1224     837
 
BsaHI Gr'CG_yC

Cuts at:      0    1187    1646    2061 
   Size:       1187     459     415

  Fragments arranged by size: 

                1187     459     415
 
BsaWI w'CCGG_w

Cuts at:      0     692    2061 
   Size:        692    1369
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     739     769    1264    1294    2061 
   Size:        739      30     495      30     767

  Fragments arranged by size: 

                 767     739     495      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     346     391     700     781     784     853    1161    1204 
   Size:        346      45     309      81       3      69     308      43

Cuts at:   1204    2061 
   Size:        857

  Fragments arranged by size: 

                 857     346     309     308      81      69      45      43
                   3
 
BseYI C'CCAG_C

Cuts at:      0     292    1090    1550    1818    2061 
   Size:        292     798     460     268     243

  Fragments arranged by size: 

                 798     460     292     268     243
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     730    1411    1783    2061 
   Size:        730     681     372     278

  Fragments arranged by size: 

                 730     681     372     278
 
BsiEI CG_ry'CG

Cuts at:      0     503    2061 
   Size:        503    1558
 
BsiHKAI G_wGCw'C

Cuts at:      0     536     992    1196    1319    2061 
   Size:        536     456     204     123     742

  Fragments arranged by size: 

                 742     536     456     204     123
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1242    2061 
   Size:       1242     819
 
Bsp1286I G_dGCh'C

Cuts at:      0      69     397     436     536     983     992    1196    1319 
   Size:         69     328      39     100     447       9     204     123

Cuts at:   1319    2061 
   Size:        742

  Fragments arranged by size: 

                 742     447     328     204     123     100      69      39
                   9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     167     752    1334    1453    2061 
   Size:        167     585     582     119     608

  Fragments arranged by size: 

                 608     585     582     167     119
 
BsrBI CCG'CTC

Cuts at:      0     505    2061 
   Size:        505    1556
 
BsrDI GCAATG_nn'

Cuts at:      0    1039    1262    2061 
   Size:       1039     223     799

  Fragments arranged by size: 

                1039     799     223
 
BsrFI r'CCGG_y

Cuts at:      0     730    1374    2061 
   Size:        730     644     687

  Fragments arranged by size: 

                 730     687     644
 
BssSI C'ACGA_G

Cuts at:      0    1353    1489    2061 
   Size:       1353     136     572

  Fragments arranged by size: 

                1353     572     136
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1770    2061 
   Size:       1770     291
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1569    1620    1758    2061 
   Size:       1569      51     138     303

  Fragments arranged by size: 

                1569     303     138      51
 
BstYI r'GATC_y

Cuts at:      0    1670    1766    2061 
   Size:       1670      96     295

  Fragments arranged by size: 

                1670     295      96
 
BtgI C'CryG_G

Cuts at:      0      69     704     983    1544    1754    2061 
   Size:         69     635     279     561     210     307

  Fragments arranged by size: 

                 635     561     307     279     210      69
 
BtsI GCAGTG_nn'

Cuts at:      0    1092    1163    1813    2061 
   Size:       1092      71     650     248

  Fragments arranged by size: 

                1092     650     248      71
 
DraIII CAC_nnn'GTG

Cuts at:      0     710    2061 
   Size:        710    1351
 
DrdI GACnn_nn'nnGTC

Cuts at:      0       9    1684    2061 
   Size:          9    1675     377

  Fragments arranged by size: 

                1675     377       9
 
EaeI y'GGCC_r

Cuts at:      0     500    1334    1739    2061 
   Size:        500     834     405     322

  Fragments arranged by size: 

                 834     500     405     322
 
EagI C'GGCC_G

Cuts at:      0     500    2061 
   Size:        500    1561
 
EarI CTCTTCn'nnn_

Cuts at:      0      55     179     823     850    1717    1993    2061 
   Size:         55     124     644      27     867     276      68

  Fragments arranged by size: 

                 867     644     276     124      68      55      27
 
EcoICRI GAG'CTC

Cuts at:      0     534    2061 
   Size:        534    1527
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     532     589     655     703     721     861    1266    1622 
   Size:        532      57      66      48      18     140     405     356

Cuts at:   1622    1698    2017    2061 
   Size:         76     319      44

  Fragments arranged by size: 

                 532     405     356     319     140      76      66      57
                  48      44      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     388     591     895    2061 
   Size:        388     203     304    1166

  Fragments arranged by size: 

                1166     388     304     203
 
EcoO109I rG'GnC_Cy

Cuts at:      0     189     255     393     394     543     596     741    2061 
   Size:        189      66     138       1     149      53     145    1320

  Fragments arranged by size: 

                1320     189     149     145     138      66      53       1
            
 
EcoRI G'AATT_C

Cuts at:      0     906    2061 
   Size:        906    1155
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     342    2061 
   Size:        342    1719
 
FspI TGC'GCA

Cuts at:      0     163    2061 
   Size:        163    1898
 
HaeII r_GCGC'y

Cuts at:      0    1544    2018    2061 
   Size:       1544     474      43

  Fragments arranged by size: 

                1544     474      43
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     288     320     331     363     661     693    2061 
   Size:        288      32      11      32     298      32    1368

  Fragments arranged by size: 

                1368     298     288      32      32      32      11
 
MscI TGG'CCA

Cuts at:      0    1741    2061 
   Size:       1741     320
 
MslI CAynn'nnrTG

Cuts at:      0    1312    1741    1756    1759    2061 
   Size:       1312     429      15       3     302

  Fragments arranged by size: 

                1312     429     302      15       3
 
MspA1I CmG'CkG

Cuts at:      0     111     402     552    2061 
   Size:        111     291     150    1509

  Fragments arranged by size: 

                1509     291     150     111
 
NaeI GCC'GGC

Cuts at:      0     732    2061 
   Size:        732    1329
 
NcoI C'CATG_G

Cuts at:      0    1544    1754    2061 
   Size:       1544     210     307

  Fragments arranged by size: 

                1544     307     210
 
NgoMIV G'CCGG_C

Cuts at:      0     730    2061 
   Size:        730    1331
 
NheI G'CTAG_C

Cuts at:      0    1746    2061 
   Size:       1746     315
 
NspI r_CATG'y

Cuts at:      0     292     528    1807    2061 
   Size:        292     236    1279     254

  Fragments arranged by size: 

                1279     292     254     236
 
PasI CC'CwG_GG

Cuts at:      0     251    1847    2061 
   Size:        251    1596     214

  Fragments arranged by size: 

                1596     251     214
 
PciI A'CATG_T

Cuts at:      0     524    1803    2061 
   Size:        524    1279     258

  Fragments arranged by size: 

                1279     524     258
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     710     921    1550    1667    2061 
   Size:        710     211     629     117     394

  Fragments arranged by size: 

                 710     629     394     211     117
 
PfoI T'CCnGG_A

Cuts at:      0     737     896    1282    1453    1673    2061 
   Size:        737     159     386     171     220     388

  Fragments arranged by size: 

                 737     388     386     220     171     159
 
PpuMI rG'GwC_Cy

Cuts at:      0     543     741    2061 
   Size:        543     198    1320

  Fragments arranged by size: 

                1320     543     198
 
PspOMI G'GGCC_C

Cuts at:      0     393    2061 
   Size:        393    1668
 
PspXI vC'TCGA_Gb

Cuts at:      0    1465    2061 
   Size:       1465     596
 
PstI C_TGCA'G

Cuts at:      0      23     106     219    1973    2061 
   Size:         23      83     113    1754      88

  Fragments arranged by size: 

                1754     113      88      83      23
 
PvuII CAG'CTG

Cuts at:      0     552    2061 
   Size:        552    1509
 
SacI G_AGCT'C

Cuts at:      0     536    2061 
   Size:        536    1525
 
SapI GCTCTTCn'nnn_

Cuts at:      0      55    1993    2061 
   Size:         55    1938      68

  Fragments arranged by size: 

                1938      68      55
 
ScaI AGT'ACT

Cuts at:      0     793    2061 
   Size:        793    1268
 
SfcI C'TryA_G

Cuts at:      0      19     102     215    1009    1969    2061 
   Size:         19      83     113     794     960      92

  Fragments arranged by size: 

                 960     794     113      92      83      19
 
SmaI CCC'GGG

Cuts at:      0    1073    1978    2061 
   Size:       1073     905      83

  Fragments arranged by size: 

                1073     905      83
 
SmlI C'TyrA_G

Cuts at:      0     588    1465    1531    2061 
   Size:        588     877      66     530

  Fragments arranged by size: 

                 877     588     530      66
 
StuI AGG'CCT

Cuts at:      0     586    2061 
   Size:        586    1475
 
StyI C'CwwG_G

Cuts at:      0      60    1544    1754    1836    1929    2061 
   Size:         60    1484     210      82      93     132

  Fragments arranged by size: 

                1484     210     132      93      82      60
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     913    1082    1804    2061 
   Size:        913     169     722     257

  Fragments arranged by size: 

                 913     722     257     169
 
TatI w'GTAC_w

Cuts at:      0     791    2061 
   Size:        791    1270
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     214     246     739     771    2061 
   Size:        214      32     493      32    1290

  Fragments arranged by size: 

                1290     493     214      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     918    2061 
   Size:        918    1143
 
XhoI C'TCGA_G

Cuts at:      0    1465    2061 
   Size:       1465     596
 
XmaI C'CCGG_G

Cuts at:      0    1071    1976    2061 
   Size:       1071     905      85

  Fragments arranged by size: 

                1071     905      85
 
XmnI GAAnn'nnTTC

Cuts at:      0      92    2061 
   Size:         92    1969
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AhdI     AleI    AlwNI     ApaI    ApaLI
     ApoI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI
     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI    BstXI    BstYI     BtgI     BtsI   DraIII     DrdI
     EaeI     EagI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I     MscI     MslI   MspA1I     NaeI
     NcoI   NgoMIV     NheI     NspI     PasI     PciI    PflMI     PfoI
    PpuMI   PspOMI    PspXI     PstI    PvuII     SacI     SapI     ScaI
     SfcI     SmaI     SmlI     StuI     StyI    TaqII     TatI     TstI
     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AlfI
     AloI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI    BciVI
     BclI    BfrBI     BglI    BglII    BmgBI    BsaBI    BsiWI     BsmI
    BspEI    BspHI    BsrGI   BssHII    BstBI   BstEII  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI     EciI    EcoRV     FseI    FspAI
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     NarI     NdeI     NotI     NruI     NsiI     PacI     PmeI     PmlI
     PpiI    PshAI     PsiI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI  Tth111I     XbaI     ZraI
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