Restriction map for pF1KB9515
##################################
# NO CUT ENZYMES (  122 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AgeI        
AhdI        Alw44I      AocI        ApaI        ApaLI       
AscI        Asp700I     AspEI       AspI        AvaI        
AviII       AvrII       BamHI       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaI        BsaWI       BseAI       
BsgI        BsiWI       BsmBI       BsmI        BsoBI       
BspDI       BspEI       BspLU11I    BsrBI       BssHII      
BstBI       BstEII      BstPI       BstXI       Bsu36I      
CelII       ClaI        CpoI        DraII       DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoT22I     FauI        FbaI        FseI        FspI        
HindIII     HpaI        KasI        KspI        MaeII       
MamI        MroI        MslI        NaeI        NarI        
NcoI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       Psp1406I    PvuI        RsrII       SacI        
SacII       SapI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        Sse8387I    StuI        SwaI        
Tth111I     XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

Acc65I        860  GGTACC               G^GTACC
AccII         517  CGCG                 CG^CG
AcyI         1447  GACGCC               G[GA]^CG[CT]C
AflIII        516  ACGCGT               A^C[GA][CT]GT
Aor51HI      1336  AGCGCT               AGC^GCT
AseI         1405  ATTAAT               AT^TAAT
AsnI         1405  ATTAAT               AT^TAAT
Asp718I       860  GGTACC               G^GTACC
AspHI        1234  GTGCTC               G[AT]GC[AT]^C
BalI         1217  TGGCCA               TGG^CCA
BanI          860  GGTACC               G^G[CT][GA]CC
BbsI          726  GAAGAC               GAAGAC(2/6)
BpuAI         726  GAAGAC               GAAGAC(2/6)
BsaHI        1447  GACGCC               G[GA]^CG[CT]C
BseRI        1010  CTCCTC               GAGGAG(10/8)
BsiEI          38  CGACCG               CG[GA][CT]^CG
BsiHKAI      1234  GTGCTC               G[AT]GC[AT]^C
Bsp1407I      869  TGTACA               T^GTACA
BspHI         450  TCATGA               T^CATGA
BspMI         663  ACCTGC               ACCTGC(4/8)
BsrDI        1505  GCAATG               GCAATG(2/0)
BsrFI          80  ACCGGC               [GA]^CCGG[CT]
BsrGI         869  TGTACA               T^GTACA
BssSI         122  CTCGTG               CACGAG(-5/-1)
Bst1107I      940  GTATAC               GTA^TAC
BstUI         517  CGCG                 CG^CG
BstZ17I       940  GTATAC               GTA^TAC
Cac8I        1289  GCATGC               GC[ACGT]^[ACGT]GC
Cfr10I         80  ACCGGC               [GA]^CCGG[CT]
DraI          484  TTTAAA               TTT^AAA
EaeI         1217  TGGCCA               [CT]^GGCC[GA]
Eco47III     1336  AGCGCT               AGC^GCT
EcoRI         799  GAATTC               G^AATTC
HaeII        1336  AGCGCT               [GA]GCGC^[CT]
Hin1I        1447  GACGCC               G[GA]^CG[CT]C
KpnI          860  GGTACC               GGTAC^C
MfeI           17  CAATTG               C^AATTG
MluI          516  ACGCGT               A^CGCGT
MluNI        1217  TGGCCA               TGG^CCA
MscI         1217  TGGCCA               TGG^CCA
MunI           17  CAATTG               C^AATTG
MvnI          517  CGCG                 CG^CG
NdeI          668  CATATG               CA^TATG
PflMI        1063  CCATTTGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          729  GACTC                GAGTC(4/5)
PshBI        1405  ATTAAT               AT^TAAT
PvuII        1163  CAGCTG               CAG^CTG
RcaI          450  TCATGA               T^CATGA
SalI           33  GTCGAC               G^TCGAC
ScaI         1305  AGTACT               AGT^ACT
SphI         1289  GCATGC               GCATG^C
SspBI         869  TGTACA               T^GTACA
SspI         1525  AATATT               AAT^ATT
TfiI          650  GATTC                G^A[AT]TC
Van91I       1063  CCATTTGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AlwNI         251  CAGTCACTG            CAG[ACGT][ACGT][ACGT]^CTG
              580  CAGTTCCTG           
AvaII         673  GGACC                G^G[AT]CC
             1055  GGTCC               
BanII           7  GAGCCC               G[GA]GC[CT]^C
              836  GAGCCC              
BcnI          786  CCCGG                CC^[GC]GG
             1172  CCCGG               
BfaI         1129  CTAG                 C^TAG
             1373  CTAG                
BpmI          608  CTCCAG               CTGGAG(16/14)
             1313  CTGGAG              
BsmAI        1486  GAGAC                GTCTC(1/5)
              606  GTCTC               
BstAPI       1159  GCAACAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1323  GCAAACCCTGC         
BstNI         558  CCTGG                CC^[AT]GG
              705  CCTGG               
BstYI         316  GGATCT               [GA]^GATC[CT]
             1196  AGATCC              
EcoRII        558  CCTGG                ^CC[AT]GG
              705  CCTGG               
EcoT14I        11  CCAAGG               C^C[AT][AT]GG
             1388  CCAAGG              
HaeIII        402  GGCC                 GG^CC
             1218  GGCC                
HgaI         1263  GACGC                GACGC(5/10)
             1447  GACGC               
HincII         33  GTCGAC               GT[CT]^[GA]AC
              358  GTTGAC              
HindII         33  GTCGAC               GT[CT]^[GA]AC
              358  GTTGAC              
HinfI         650  GATTC                G^A[ACGT]TC
              729  GACTC               
MaeI         1129  CTAG                 C^TAG
             1373  CTAG                
MflI          316  GGATCT               [GA]^GATC[CT]
             1196  AGATCC              
MspA1I       1163  CAGCTG               C[AC]G^C[GT]G
             1299  CAGCGG              
MvaI          558  CCTGG                CC^[AT]GG
              705  CCTGG               
NciI          786  CCCGG                CC^[GC]GG
             1172  CCCGG               
NlaIV         860  GGTACC               GG[ACGT]^[ACGT]CC
             1055  GGTCCC              
NspI          866  GCATGT               [GA]CATG^[CT]
             1289  GCATGC              
PstI          956  CTGCAG               CTGCA^G
             1166  CTGCAG              
StyI           11  CCAAGG               C^C[AT][AT]GG
             1388  CCAAGG              
XhoII         316  GGATCT               [GA]^GATC[CT]
             1196  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   54 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        ApoI        BbvI        BmyI        BsaJI       
BsiYI       BslI        BsmFI       Bsp1286I    BsrI        
BssKI       BstF5I      CfoI        Cfr13I      DdeI        
DpnII       EarI        EcoRV       Fnu4HI      FokI        
HapII       HhaI        HinP1I      HpaII       HphI        
ItaI        Ksp632I     MaeIII      MboI        MboII       
MnlI        MseI        MspI        MwoI        NdeII       
NlaIII      RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        TaqI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     
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