Restriction map for pF1KB9502
##################################
# NO CUT ENZYMES (   95 enzymes) #
##################################

AatII       AccIII      AclI        AcyI        AflII       
AgeI        AhdI        AocI        Aor51HI     ApaI        
AscI        AseI        AsnI        AspEI       AviII       
AvrII       BbeI        BbvCI       BcgI        BfrI        
BlnI        BlpI        Bpu1102I    BsaHI       BsaWI       
BseAI       BseRI       BsiEI       BsiWI       BsmBI       
BspEI       BsrBI       BsrDI       BsrFI       BssHII      
BssSI       BstAPI      BstBI       BstXI       Bsu36I      
CelII       Cfr10I      CpoI        DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoRV       EcoT22I     FseI        FspI        Hin1I       
HincII      HindII      HpaI        KasI        KspI        
MluI        MroI        NaeI        NarI        NdeI        
NgoMIV      NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacI        
SacII       SalI        SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
Sse8387I    StuI        SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccII          68  CGCG                 CG^CG
Alw44I       1772  GTGCAC               G^TGCAC
AlwNI         611  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI        1772  GTGCAC               G^TGCAC
AspI         3424  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI           51  CCCGGG               C^[CT]CG[GA]G
BamHI        2543  GGATCC               G^GATCC
BanII         324  GAGCCC               G[GA]GC[CT]^C
BbrPI        3023  CACGTG               CAC^GTG
BclI         2208  TGATCA               T^GATCA
BglI           18  GCCCGCTAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI        3023  CACGTG               [CT]AC^GT[GA]
BsaI           81  GAGACC               GGTCTC(1/5)
BsmI         1539  GCATTC               GAATGC(1/-1)
BsoBI          51  CCCGGG               C^[CT]CG[GA]G
Bsp1407I     2770  TGTACA               T^GTACA
BspDI         503  ATCGAT               AT^CGAT
BsrGI        2770  TGTACA               T^GTACA
BstEII       2791  GGTTACC              G^GT[ACGT]ACC
BstPI        2791  GGTTACC              G^GT[ACGT]ACC
BstUI          68  CGCG                 CG^CG
ClaI          503  ATCGAT               AT^CGAT
DraII        1626  GGGACCT              [GA]G^G[ACGT]CC[CT]
DsaI         2539  CCATGG               C^C[GA][CT]GG
EcoNI        1729  CCTTCAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     1626  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoO65I      2791  GGTTACC              G^GT[ACGT]ACC
EcoRI         792  GAATTC               G^AATTC
FbaI         2208  TGATCA               T^GATCA
HaeII           8  GGCGCT               [GA]GCGC^[CT]
HgaI         1089  GCGTC                GACGC(5/10)
MfeI         2192  CAATTG               C^AATTG
MunI         2192  CAATTG               C^AATTG
MvnI           68  CGCG                 CG^CG
NcoI         2539  CCATGG               C^CATGG
PflFI        3424  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1438  CCAAGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        3023  CACGTG               CAC^GTG
PmlI         3023  CACGTG               CAC^GTG
PpuMI        1626  GGGACCT              [GA]G^G[AT]CC[CT]
SexAI        1146  ACCTGGT              A^CC[AT]GGT
SmaI           51  CCCGGG               CCC^GGG
SphI           14  GCATGC               GCATG^C
SspBI        2770  TGTACA               T^GTACA
Tth111I      3424  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1438  CCAAGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI          51  CCCGGG               C^CCGGG
XmaI           51  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

Acc65I       1288  GGTACC               G^GTACC
             3507  GGTACC              
AflIII        191  ACATGT               A^C[GA][CT]GT
             1974  ACATGT              
Asp718I      1288  GGTACC               G^GTACC
             3507  GGTACC              
BalI         1245  TGGCCA               TGG^CCA
             2536  TGGCCA              
BciVI        3437  GGATAC               GTATCC(6/5)
             1694  GTATCC              
BsaBI         559  GATAGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2209  GATCATCATC          
BsgI         2470  GTGCAG               GTGCAG(16/14)
             3145  GTGCAG              
BspLU11I      191  ACATGT               A^CATGT
             1974  ACATGT              
Bst1107I      380  GTATAC               GTA^TAC
             2268  GTATAC              
BstZ17I       380  GTATAC               GTA^TAC
             2268  GTATAC              
DraI         2742  TTTAAA               TTT^AAA
             2833  TTTAAA              
DraIII       1145  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3334  CACTTTGTG           
EaeI         1245  TGGCCA               [CT]^GGCC[GA]
             2536  TGGCCA              
HindIII       338  AAGCTT               A^AGCTT
             3070  AAGCTT              
KpnI         1288  GGTACC               GGTAC^C
             3507  GGTACC              
MamI          559  GATAGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2209  GATCATCATC          
MluNI        1245  TGGCCA               TGG^CCA
             2536  TGGCCA              
MscI         1245  TGGCCA               TGG^CCA
             2536  TGGCCA              
NheI         1357  GCTAGC               G^CTAGC
             1384  GCTAGC              
SmlI          930  CTCAAG               C^T[CT][GA]AG
             1081  CTTGAG              
SspI          422  AATATT               AAT^ATT
             2229  AATATT              
TaqI          504  TCGA                 T^CGA
              567  TCGA                
TthHB8I       504  TCGA                 T^CGA
              567  TCGA                
XcmI          366  CCATGAAGATCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2530  CCAGTTTGGCCATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   91 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        ApoI        Asp700I     AspHI       AvaII       
BanI        BbsI        BbvI        BcnI        BfaI        
BglII       BmyI        BpmI        BpuAI       BsaJI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspHI       BspMI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       EarI        EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PleI        PstI        PvuII       RcaI        
RsaI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       XhoII       
XmnI        
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