Restriction map for pF1KB9502
 (Linear) (Six-base) MAPSORT of: KIBB9502.seq  Check: 5487  from: 1  to: 3519



KIBB9502.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:39  ..

AccI GT'mk_AC

Cuts at:      0     381    2269    2876    3519 
   Size:        381    1888     607     643

  Fragments arranged by size: 

                1888     643     607     381
 
Acc65I G'GTAC_C

Cuts at:      0    1288    3507    3519 
   Size:       1288    2219      12

  Fragments arranged by size: 

                2219    1288      12
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     953    1159    1351    1592    1700    1715    2502    2608 
   Size:        953     206     192     241     108      15     787     106

Cuts at:   2608    3519 
   Size:        911

  Fragments arranged by size: 

                 953     911     787     241     206     192     108     106
                  15
 
AflIII A'CryG_T

Cuts at:      0     191    1974    3519 
   Size:        191    1783    1545

  Fragments arranged by size: 

                1783    1545     191
 
AleI CACnn'nnGTG

Cuts at:      0    2932    3519 
   Size:       2932     587
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     111     143    3519 
   Size:        111      32    3376

  Fragments arranged by size: 

                3376     111      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     616    3519 
   Size:        616    2903
 
ApaLI G'TGCA_C

Cuts at:      0    1772    3519 
   Size:       1772    1747
 
ApoI r'AATT_y

Cuts at:      0     703     792     991    1117    1230    1616    1752    2167 
   Size:        703      89     199     126     113     386     136     415

Cuts at:   2167    2179    2255    3519 
   Size:         12      76    1264

  Fragments arranged by size: 

                1264     703     415     386     199     136     126     113
                  89      76      12
 
AvaI C'yCGr_G

Cuts at:      0      51    3519 
   Size:         51    3468
 
BamHI G'GATC_C

Cuts at:      0    2543    3519 
   Size:       2543     976
 
BanI G'GyrC_C

Cuts at:      0     309     671    1221    1288    2934    3507    3519 
   Size:        309     362     550      67    1646     573      12

  Fragments arranged by size: 

                1646     573     550     362     309      67      12
 
BanII G_rGCy'C

Cuts at:      0     328    3519 
   Size:        328    3191
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1147    2036    2078    2129    2648    3220    3519 
   Size:       1147     889      42      51     519     572     299

  Fragments arranged by size: 

                1147     889     572     519     299      51      42
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1705    3431    3519 
   Size:       1705    1726      88

  Fragments arranged by size: 

                1726    1705      88
 
BclI T'GATC_A

Cuts at:      0    2208    3519 
   Size:       2208    1311
 
BglI GCCn_nnn'nGGC

Cuts at:      0      24    3519 
   Size:         24    3495
 
BglII A'GATC_T

Cuts at:      0    1029    1948    2599    3519 
   Size:       1029     919     651     920

  Fragments arranged by size: 

                1029     920     919     651
 
Bme1580I G_kGCm'C

Cuts at:      0    1776    3519 
   Size:       1776    1743
 
BmgBI CAC'GTC

Cuts at:      0     394    3519 
   Size:        394    3125
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     260     703    3519 
   Size:        260     443    2816

  Fragments arranged by size: 

                2816     443     260
 
BmtI G_CTAG'C

Cuts at:      0    1361    1388    3519 
   Size:       1361      27    2131

  Fragments arranged by size: 

                2131    1361      27
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1533    3519 
   Size:       1533    1986
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     621    2635    2952    3519 
   Size:        621    2014     317     567

  Fragments arranged by size: 

                2014     621     567     317
 
Bpu10I CC'TnA_GC

Cuts at:      0     646    3519 
   Size:        646    2873
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     915    1102    3519 
   Size:        915     187    2417

  Fragments arranged by size: 

                2417     915     187
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      75    3519 
   Size:         75    3444
 
BsaAI yAC'GTr

Cuts at:      0    3025    3519 
   Size:       3025     494
 
BsaBI GATnn'nnATC

Cuts at:      0     563    2213    3519 
   Size:        563    1650    1306

  Fragments arranged by size: 

                1650    1306     563
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     469     499    1647    1677    3519 
   Size:        469      30    1148      30    1842

  Fragments arranged by size: 

                1842    1148     469      30      30
 
BseYI C'CCAG_C

Cuts at:      0      45    3519 
   Size:         45    3474
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2491    3166    3519 
   Size:       2491     675     353

  Fragments arranged by size: 

                2491     675     353
 
BsiHKAI G_wGCw'C

Cuts at:      0     365    1485    1776    2162    3519 
   Size:        365    1120     291     386    1357

  Fragments arranged by size: 

                1357    1120     386     365     291
 
BsmI GAATG_Cn'

Cuts at:      0    1539    3519 
   Size:       1539    1980
 
Bsp1286I G_dGCh'C

Cuts at:      0     328     365    1485    1776    2162    3519 
   Size:        328      37    1120     291     386    1357

  Fragments arranged by size: 

                1357    1120     386     328     291      37
 
BspHI T'CATG_A

Cuts at:      0     344     537    3495    3519 
   Size:        344     193    2958      24

  Fragments arranged by size: 

                2958     344     193      24
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1276    1753    3108    3519 
   Size:       1276     477    1355     411

  Fragments arranged by size: 

                1355    1276     477     411
 
BsrGI T'GTAC_A

Cuts at:      0    2770    3519 
   Size:       2770     749
 
BstEII G'GTnAC_C

Cuts at:      0    2791    3519 
   Size:       2791     728
 
BstYI r'GATC_y

Cuts at:      0     940    1029    1948    2543    2599    3519 
   Size:        940      89     919     595      56     920

  Fragments arranged by size: 

                 940     920     919     595      89      56
 
BstZ17I GTA'TAC

Cuts at:      0     382    2270    3519 
   Size:        382    1888    1249

  Fragments arranged by size: 

                1888    1249     382
 
BtgI C'CryG_G

Cuts at:      0    2539    3519 
   Size:       2539     980
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    3118    3519 
   Size:       3118     401
 
BtsI GCAGTG_nn'

Cuts at:      0    1776    3519 
   Size:       1776    1743
 
ClaI AT'CG_AT

Cuts at:      0     504    3519 
   Size:        504    3015
 
DraI TTT'AAA

Cuts at:      0    2744    2835    3519 
   Size:       2744      91     684

  Fragments arranged by size: 

                2744     684      91
 
DraIII CAC_nnn'GTG

Cuts at:      0    1150    3339    3519 
   Size:       1150    2189     180

  Fragments arranged by size: 

                2189    1150     180
 
EaeI y'GGCC_r

Cuts at:      0    1245    2536    3519 
   Size:       1245    1291     983

  Fragments arranged by size: 

                1291    1245     983
 
EarI CTCTTCn'nnn_

Cuts at:      0     951    1212    1490    1844    2150    3519 
   Size:        951     261     278     354     306    1369

  Fragments arranged by size: 

                1369     951     354     306     278     261
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     621     953    1159    1351    1592    1700    1715    2502 
   Size:        621     332     206     192     241     108      15     787

Cuts at:   2502    2608    2635    2952    3519 
   Size:        106      27     317     567

  Fragments arranged by size: 

                 787     621     567     332     317     241     206     192
                 108     106      27      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1733    3519 
   Size:       1733    1786
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1627    3519 
   Size:       1627    1892
 
EcoRI G'AATT_C

Cuts at:      0     792    3519 
   Size:        792    2727
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     962    2392    3519 
   Size:        962    1430    1127

  Fragments arranged by size: 

                1430    1127     962
 
HaeII r_GCGC'y

Cuts at:      0      12    3519 
   Size:         12    3507
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     230     262     469     501    2584    2616    3032    3064 
   Size:        230      32     207      32    2083      32     416      32

Cuts at:   3064    3519 
   Size:        455

  Fragments arranged by size: 

                2083     455     416     230     207      32      32      32
                  32
 
HindIII A'AGCT_T

Cuts at:      0     338    3070    3519 
   Size:        338    2732     449

  Fragments arranged by size: 

                2732     449     338
 
KpnI G_GTAC'C

Cuts at:      0    1292    3511    3519 
   Size:       1292    2219       8

  Fragments arranged by size: 

                2219    1292       8
 
MfeI C'AATT_G

Cuts at:      0    2192    3519 
   Size:       2192    1327
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     176    3519 
   Size:        176    3343
 
MscI TGG'CCA

Cuts at:      0    1247    2538    3519 
   Size:       1247    1291     981

  Fragments arranged by size: 

                1291    1247     981
 
MslI CAynn'nnrTG

Cuts at:      0    2219    2932    3092    3519 
   Size:       2219     713     160     427

  Fragments arranged by size: 

                2219     713     427     160
 
MspA1I CmG'CkG

Cuts at:      0     109    2569    2856    3003    3519 
   Size:        109    2460     287     147     516

  Fragments arranged by size: 

                2460     516     287     147     109
 
NcoI C'CATG_G

Cuts at:      0    2539    3519 
   Size:       2539     980
 
NheI G'CTAG_C

Cuts at:      0    1357    1384    3519 
   Size:       1357      27    2135

  Fragments arranged by size: 

                2135    1357      27
 
NspI r_CATG'y

Cuts at:      0      18     195    1978    3370    3519 
   Size:         18     177    1783    1392     149

  Fragments arranged by size: 

                1783    1392     177     149      18
 
PciI A'CATG_T

Cuts at:      0     191    1974    3519 
   Size:        191    1783    1545

  Fragments arranged by size: 

                1783    1545     191
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1444    3519 
   Size:       1444    2075
 
PfoI T'CCnGG_A

Cuts at:      0    1983    3519 
   Size:       1983    1536
 
PmlI CAC'GTG

Cuts at:      0    3025    3519 
   Size:       3025     494
 
PpuMI rG'GwC_Cy

Cuts at:      0    1627    3519 
   Size:       1627    1892
 
PsiI TTA'TAA

Cuts at:      0    1123    2705    3519 
   Size:       1123    1582     814

  Fragments arranged by size: 

                1582    1123     814
 
PstI C_TGCA'G

Cuts at:      0     108     733    1215    1710    1911    2057    3119    3519 
   Size:        108     625     482     495     201     146    1062     400

  Fragments arranged by size: 

                1062     625     495     482     400     201     146     108
            
 
PvuII CAG'CTG

Cuts at:      0    2569    2856    3003    3519 
   Size:       2569     287     147     516

  Fragments arranged by size: 

                2569     516     287     147
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1212    1490    2150    3519 
   Size:       1212     278     660    1369

  Fragments arranged by size: 

                1369    1212     660     278
 
SexAI A'CCwGG_T

Cuts at:      0    1146    3519 
   Size:       1146    2373
 
SfcI C'TryA_G

Cuts at:      0     104     729    1211    1544    1706    1907    1913    2053 
   Size:        104     625     482     333     162     201       6     140

Cuts at:   2053    2312    3115    3519 
   Size:        259     803     404

  Fragments arranged by size: 

                 803     625     482     404     333     259     201     162
                 140     104       6
 
SmaI CCC'GGG

Cuts at:      0      53    3519 
   Size:         53    3466
 
SmlI C'TyrA_G

Cuts at:      0     930    1081    3519 
   Size:        930     151    2438

  Fragments arranged by size: 

                2438     930     151
 
SphI G_CATG'C

Cuts at:      0      18    3519 
   Size:         18    3501
 
SspI AAT'ATT

Cuts at:      0     424    2231    3519 
   Size:        424    1807    1288

  Fragments arranged by size: 

                1807    1288     424
 
StyI C'CwwG_G

Cuts at:      0     853    1178    1292    1937    2273    2539    3519 
   Size:        853     325     114     645     336     266     980

  Fragments arranged by size: 

                 980     853     645     336     325     266     114
 
TatI w'GTAC_w

Cuts at:      0    2128    2482    2770    3519 
   Size:       2128     354     288     749

  Fragments arranged by size: 

                2128     749     354     288
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2357    2389    2673    2705    3009    3041    3519 
   Size:       2357      32     284      32     304      32     478

  Fragments arranged by size: 

                2357     478     304     284      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    3427    3519 
   Size:       3427      92
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     373    2537    3519 
   Size:        373    2164     982

  Fragments arranged by size: 

                2164     982     373
 
XmaI C'CCGG_G

Cuts at:      0      51    3519 
   Size:         51    3468
 
XmnI GAAnn'nnTTC

Cuts at:      0     796     991    3095    3519 
   Size:        796     195    2104     424

  Fragments arranged by size: 

                2104     796     424     195
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI   AflIII     AleI     AloI    AlwNI    ApaLI
     ApoI     AvaI    BamHI     BanI    BanII     BbsI    BciVI     BclI
     BglI    BglII Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaXI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrGI   BstEII    BstYI
  BstZ17I     BtgI    BtgZI     BtsI     ClaI     DraI   DraIII     EaeI
     EarI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    HaeII    Hin4I
  HindIII     KpnI     MfeI     MmeI     MscI     MslI   MspA1I     NcoI
     NheI     NspI     PciI    PflMI     PfoI     PmlI    PpuMI     PsiI
     PstI    PvuII     SapI    SexAI     SfcI     SmaI     SmlI     SphI
     SspI     StyI     TatI     TstI  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII     AgeI     AhdI     AlfI
     ApaI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI    BbvCI
     BcgI    BfrBI     BlpI    BsaHI    BsaWI    BseRI    BsiEI    BsiWI
    BsmBI    BspEI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI
    BstBI    BstXI   Bsu36I    CspCI     DrdI     EagI     EciI  EcoICRI
    EcoRV     FseI     FspI    FspAI   HincII     HpaI     KasI     MluI
     NaeI     NarI     NdeI   NgoMIV     NotI     NruI     NsiI     PacI
     PasI     PmeI     PpiI    PshAI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SrfI     StuI     SwaI
    TaqII     XbaI     XhoI     ZraI
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