Restriction map for
pF1KB9493
(Circular) (Six-base) MAPSORT of: pF1KB9493.seq Check: 3715 from: 1 to: 3704
pF1KB9493 3704 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 742 742
Size: 3704
Acc65I G'GTAC_C
Cuts at: 720 720
Size: 3704
AclI AA'CG_TT
Cuts at: 3544 3544
Size: 3704
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 329 1529 1961 2314 2976 196
Size: 133 1200 432 353 662 924
Fragments arranged by size:
1200 924 662 432 353 133
AflIII A'CryG_T
Cuts at: 273 1056 2856 3137 273
Size: 783 1800 281 840
Fragments arranged by size:
1800 840 783 281
AlwNI CAG_nnn'CTG
Cuts at: 1009 2447 1009
Size: 1438 2266
ApaLI G'TGCA_C
Cuts at: 2542 2542
Size: 3704
ApoI r'AATT_y
Cuts at: 241 708 3057 3313 241
Size: 467 2349 256 632
Fragments arranged by size:
2349 632 467 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3704
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3704
AvaI C'yCGr_G
Cuts at: 380 724 380
Size: 344 3360
BamHI G'GATC_C
Cuts at: 729 729
Size: 3704
BanI G'GyrC_C
Cuts at: 720 1134 1382 1417 720
Size: 414 248 35 3007
Fragments arranged by size:
3007 414 248 35
BanII G_rGCy'C
Cuts at: 718 1748 718
Size: 1030 2674
BbeI G_GCGC'C
Cuts at: 1138 1386 1138
Size: 248 3456
BciVI GTATCCnnnnn_n'
Cuts at: 1602 2658 3704 1602
Size: 1056 1046 1602
Fragments arranged by size:
1602 1056 1046
BclI T'GATC_A
Cuts at: 1224 1224
Size: 3704
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3704
BglII A'GATC_T
Cuts at: 1219 3256 1219
Size: 2037 1667
BlpI GC'TnA_GC
Cuts at: 816 816
Size: 3704
Bme1580I G_kGCm'C
Cuts at: 1329 1422 2546 1329
Size: 93 1124 2487
Fragments arranged by size:
2487 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 916 997 1332 3476 916
Size: 81 335 2144 1144
Fragments arranged by size:
2144 1144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 541 2071 541
Size: 1530 2174
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 157
Size: 3704
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 880 1759 2473 2771 880
Size: 879 714 298 1813
Fragments arranged by size:
1813 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3408 3408
Size: 3704
BsaAI yAC'GTr
Cuts at: 1057 1057
Size: 3704
BsaBI GATnn'nnATC
Cuts at: 6 1218 3255 6
Size: 1212 2037 455
Fragments arranged by size:
2037 1212 455
BsaHI Gr'CG_yC
Cuts at: 1135 1383 2085 3581 1135
Size: 248 702 1496 1258
Fragments arranged by size:
1496 1258 702 248
BsaWI w'CCGG_w
Cuts at: 893 1414 2092 2503 2650 2924 893
Size: 521 678 411 147 274 1673
Fragments arranged by size:
1673 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 533 563 533
Size: 30 3674
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 517 517
Size: 3704
BseYI C'CCAG_C
Cuts at: 1129 1887 2552 2960 1129
Size: 758 665 408 1873
Fragments arranged by size:
1873 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1246 1292 2522 104
Size: 1142 46 1230 1286
Fragments arranged by size:
1286 1230 1142 46
BsiHKAI G_wGCw'C
Cuts at: 718 1496 1686 2546 718
Size: 778 190 860 1876
Fragments arranged by size:
1876 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 718 1329 1422 1496 1686 1748 2546 718
Size: 611 93 74 190 62 798 1876
Fragments arranged by size:
1876 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 564 2136 564
Size: 1572 2132
BspMI ACCTGCnnnn'nnnn_
Cuts at: 754 968 1270 1651 754
Size: 214 302 381 2807
Fragments arranged by size:
2807 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1996 2050 3534 3699 50
Size: 1946 54 1484 165 55
Fragments arranged by size:
1946 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 255 1616 255
Size: 1361 2343
BsrFI r'CCGG_y
Cuts at: 1702 1883 1702
Size: 181 3523
BssSI C'ACGA_G
Cuts at: 1975 2683 1975
Size: 708 2996
BstAPI GCAn_nnn'nTGC
Cuts at: 355 1081 355
Size: 726 2978
BstBI TT'CG_AA
Cuts at: 2065 2065
Size: 3704
BstYI r'GATC_y
Cuts at: 1 729 1196 1219 1554 1800 2106 2118 2204
Size: 728 467 23 335 246 306 12 86
Cuts at: 2204 3256 1
Size: 1052 449
Fragments arranged by size:
1052 728 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3704
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1654 1835 1998 1654
Size: 181 163 3360
Fragments arranged by size:
3360 181 163
BtsI GCAGTG_nn'
Cuts at: 947 947
Size: 3704
DraI TTT'AAA
Cuts at: 703 703
Size: 3704
DrdI GACnn_nn'nnGTC
Cuts at: 1410 2754 1410
Size: 1344 2360
EaeI y'GGCC_r
Cuts at: 1289 1463 1854 1881 3226 1289
Size: 174 391 27 1345 1767
Fragments arranged by size:
1767 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1289 1289
Size: 3704
EarI CTCTTCn'nnn_
Cuts at: 401 1727 1937 401
Size: 1326 210 2168
Fragments arranged by size:
2168 1326 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1912 2654 2800 3514 1912
Size: 742 146 714 2102
Fragments arranged by size:
2102 742 714 146
EcoICRI GAG'CTC
Cuts at: 716 716
Size: 3704
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 196 329 1529 1961 2314 2976 157
Size: 39 133 1200 432 353 662 885
Fragments arranged by size:
1200 885 662 432 353 133 39
EcoO109I rG'GnC_Cy
Cuts at: 411 504 843 411
Size: 93 339 3272
Fragments arranged by size:
3272 339 93
EcoRI G'AATT_C
Cuts at: 241 708 241
Size: 467 3237
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 430 1723 430
Size: 1293 2411
FspI TGC'GCA
Cuts at: 1485 1485
Size: 3704
HaeII r_GCGC'y
Cuts at: 118 1138 1386 2616 3387 118
Size: 1020 248 1230 771 435
Fragments arranged by size:
1230 1020 771 435 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1546 1576 1578 1608 1546
Size: 30 2 30 3642
Fragments arranged by size:
3642 30 30 2
HincII GTy'rAC
Cuts at: 743 743
Size: 3704
KasI G'GCGC_C
Cuts at: 1134 1382 1134
Size: 248 3456
KpnI G_GTAC'C
Cuts at: 724 724
Size: 3704
MfeI C'AATT_G
Cuts at: 158 158
Size: 3704
MluI A'CGCG_T
Cuts at: 3137 3137
Size: 3704
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2463 2647 2463
Size: 184 3520
MscI TGG'CCA
Cuts at: 1465 1465
Size: 3704
MslI CAynn'nnrTG
Cuts at: 278 1693 1820 278
Size: 1415 127 2162
Fragments arranged by size:
2162 1415 127
MspA1I CmG'CkG
Cuts at: 815 1129 1489 2273 2518 815
Size: 314 360 784 245 2001
Fragments arranged by size:
2001 784 360 314 245
NaeI GCC'GGC
Cuts at: 1885 1885
Size: 3704
NarI GG'CG_CC
Cuts at: 1135 1383 1135
Size: 248 3456
NcoI C'CATG_G
Cuts at: 107 107
Size: 3704
NgoMIV G'CCGG_C
Cuts at: 1883 1883
Size: 3704
NspI r_CATG'y
Cuts at: 277 757 1257 2860 277
Size: 480 500 1603 1121
Fragments arranged by size:
1603 1121 500 480
PciI A'CATG_T
Cuts at: 273 2856 273
Size: 2583 1121
PmeI GTTT'AAAC
Cuts at: 703 703
Size: 3704
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2150 2182 2150
Size: 32 3672
PspXI vC'TCGA_Gb
Cuts at: 380 380
Size: 3704
PstI C_TGCA'G
Cuts at: 751 1436 751
Size: 685 3019
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3704
PvuII CAG'CTG
Cuts at: 1129 1489 1129
Size: 360 3344
RsrII CG'GwC_CG
Cuts at: 1899 1899
Size: 3704
SacI G_AGCT'C
Cuts at: 718 718
Size: 3704
SalI G'TCGA_C
Cuts at: 741 741
Size: 3704
SapI GCTCTTCn'nnn_
Cuts at: 1727 1937 1727
Size: 210 3494
SbfI CC_TGCA'GG
Cuts at: 751 751
Size: 3704
ScaI AGT'ACT
Cuts at: 3221 3221
Size: 3704
SfcI C'TryA_G
Cuts at: 33 575 747 1432 2400 2591 33
Size: 542 172 685 968 191 1146
Fragments arranged by size:
1146 968 685 542 191 172
SfoI GGC'GCC
Cuts at: 1136 1384 1136
Size: 248 3456
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3704
SmaI CCC'GGG
Cuts at: 726 726
Size: 3704
SmlI C'TyrA_G
Cuts at: 380 859 1774 2488 2750 380
Size: 479 915 714 262 1334
Fragments arranged by size:
1334 915 714 479 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3704
SphI G_CATG'C
Cuts at: 757 1257 757
Size: 500 3204
SspI AAT'ATT
Cuts at: 426 426
Size: 3704
StyI C'CwwG_G
Cuts at: 107 414 838 1192 107
Size: 307 424 354 2619
Fragments arranged by size:
2619 424 354 307
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 645 1287 1420 1879 2088 645
Size: 642 133 459 209 2261
Fragments arranged by size:
2261 642 459 209 133
TatI w'GTAC_w
Cuts at: 3219 3219
Size: 3704
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3221 3253 3221
Size: 32 3672
Tth111I GACn'n_nGTC
Cuts at: 1501 1501
Size: 3704
XbaI T'CTAG_A
Cuts at: 735 735
Size: 3704
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1199 1199
Size: 3704
XhoI C'TCGA_G
Cuts at: 380 380
Size: 3704
XmaI C'CCGG_G
Cuts at: 724 724
Size: 3704
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI
BfrBI BmgBI BmtI Bpu10I BsgI BsiWI BsmI BsmBI
BspEI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XmnI ZraI