Restriction map for pF1KB9486
##################################
# NO CUT ENZYMES (   72 enzymes) #
##################################

AatII       AclI        AgeI        AhdI        AscI        
AseI        AsnI        AspEI       AviII       AvrII       
BbrPI       BciVI       BglII       BlnI        BlpI        
Bpu1102I    BsaBI       BsiWI       BsrBI       BssHII      
Bst1107I    BstZ17I     CelII       CpoI        DrdI        
EagI        Eam1105I    EclXI       Eco52I      EcoRV       
FspI        HincII      HindII      HindIII     HpaI        
MamI        MfeI        MluI        MunI        NcoI        
NdeI        NheI        NotI        NruI        PacI        
PaeR7I      PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshBI       Psp1406I    RsrII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SgrAI       SmaI        SnaBI       SphI        
Sse8387I    SwaI        Van91I      XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

Acc65I        638  GGTACC               G^GTACC
AccIII       1518  TCCGGA               T^CCGGA
AflII         334  CTTAAG               C^TTAAG
Alw44I       3402  GTGCAC               G^TGCAC
AocI         1465  CCTCAGG              CC^T[ACGT]AGG
Aor51HI      2465  AGCGCT               AGC^GCT
ApaLI        3402  GTGCAC               G^TGCAC
Asp718I       638  GGTACC               G^GTACC
AspI         3432  GACCCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI           83  TGGCCA               TGG^CCA
BamHI        3552  GGATCC               G^GATCC
BbsI         1422  GTCTTC               GAAGAC(2/6)
BfrI          334  CTTAAG               C^TTAAG
BglI           28  GCCTTCCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI        1422  GTCTTC               GAAGAC(2/6)
BseAI        1518  TCCGGA               T^CCGGA
BspDI        2039  ATCGAT               AT^CGAT
BspEI        1518  TCCGGA               T^CCGGA
BspLU11I     1810  ACATGT               A^CATGT
BssSI         892  CTCGTG               CACGAG(-5/-1)
BstAPI       3641  GCAAGTCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        2372  TTCGAA               TT^CGAA
BstEII        586  GGTCACC              G^GT[ACGT]ACC
BstPI         586  GGTCACC              G^GT[ACGT]ACC
Bsu36I       1465  CCTCAGG              CC^T[ACGT]AGG
ClaI         2039  ATCGAT               AT^CGAT
DraI          985  TTTAAA               TTT^AAA
DraIII       1554  CACACAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III     2465  AGCGCT               AGC^GCT
Eco81I       1465  CCTCAGG              CC^T[ACGT]AGG
EcoO65I       586  GGTCACC              G^GT[ACGT]ACC
EcoRI         523  GAATTC               G^AATTC
EcoT22I       701  ATGCAT               ATGCA^T
FseI         3460  GGCCGGCC             GGCCGG^CC
KpnI          638  GGTACC               GGTAC^C
KspI          128  CCGCGG               CCGC^GG
MluNI          83  TGGCCA               TGG^CCA
MroI         1518  TCCGGA               T^CCGGA
MscI           83  TGGCCA               TGG^CCA
NsiI          701  ATGCAT               ATGCA^T
NspV         2372  TTCGAA               TT^CGAA
PflFI        3432  GACCCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PvuI          262  CGATCG               CGAT^CG
SacII         128  CCGCGG               CCGC^GG
SfuI         2372  TTCGAA               TT^CGAA
SpeI         2163  ACTAGT               A^CTAGT
Tth111I      3432  GACCCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccI          205  GTAGAC               GT^[AC][GT]AC
              477  GTCTAC              
AcyI          157  GGCGCC               G[GA]^CG[CT]C
             2317  GGCGCC              
AflIII        481  ACGTGT               A^C[GA][CT]GT
             1810  ACATGT              
Asp700I      2883  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3243  GAAGGGCTTC          
BbeI          157  GGCGCC               GGCGC^C
             2317  GGCGCC              
BcgI         3186  CGAGTGTTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3334  CGACGGGCTTGC        
BsaAI         480  TACGTG               [CT]AC^GT[GA]
              598  CACGTA              
BsaHI         157  GGCGCC               G[GA]^CG[CT]C
             2317  GGCGCC              
BsiEI         122  CGGTCG               CG[GA][CT]^CG
              262  CGATCG              
BsmBI        2658  CGTCTC               CGTCTC(1/5)
             2515  GAGACG              
BsmI          273  GAATGC               GAATGC(1/-1)
             2913  GCATTC              
Bsp1407I      605  TGTACA               T^GTACA
             3359  TGTACA              
BsrFI        3155  GCCGGC               [GA]^CCGG[CT]
             3461  GCCGGC              
BsrGI         605  TGTACA               T^GTACA
             3359  TGTACA              
BstXI         372  CCAGGTACTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2809  CCAGGTGAATGG        
Cfr10I       3155  GCCGGC               [GA]^CCGG[CT]
             3461  GCCGGC              
EcoNI         806  CCTATCTAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3048  CCTCTGCAAGG         
Hin1I         157  GGCGCC               G[GA]^CG[CT]C
             2317  GGCGCC              
KasI          157  GGCGCC               G^GCGCC
             2317  GGCGCC              
NaeI         3155  GCCGGC               GCC^GGC
             3461  GCCGGC              
NarI          157  GGCGCC               GG^CGCC
             2317  GGCGCC              
NgoMIV       3155  GCCGGC               G^CCGGC
             3461  GCCGGC              
NspI         1810  ACATGT               [GA]CATG^[CT]
             2824  ACATGC              
PshAI        2517  GACGCTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             2993  GACCCTGGTC          
SacI          831  GAGCTC               GAGCT^C
             1744  GAGCTC              
SfoI          157  GGCGCC               GGC^GCC
             2317  GGCGCC              
SspBI         605  TGTACA               T^GTACA
             3359  TGTACA              
StuI         2256  AGGCCT               AGG^CCT
             2834  AGGCCT              
TfiI         1332  GATTC                G^A[AT]TC
             1350  GAATC               
XcmI          633  CCAGCGGTACCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2554  CCAGGGATAGCTTGG     
XmnI         2883  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3243  GAAGGGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  108 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApaI        ApoI        AspHI       
AvaI        AvaII       BanI        BanII       BbvCI       
BbvI        BclI        BcnI        BfaI        BmyI        
BpmI        BsaI        BsaJI       BsaWI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspHI       BspMI       
BsrDI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
FbaI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        PstI        PvuII       
RcaI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SspI        StyI        
TaqI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       
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