Restriction map for pF1KB9486
 (Linear) (Six-base) MAPSORT of: KIBB9486.seq  Check: 2685  from: 1  to: 3663



KIBB9486.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:39  ..

AccI GT'mk_AC

Cuts at:      0     206     478    3663 
   Size:        206     272    3185

  Fragments arranged by size: 

                3185     272     206
 
Acc65I G'GTAC_C

Cuts at:      0     638    3663 
   Size:        638    3025
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     380    1388    3663 
   Size:        380    1008    2275

  Fragments arranged by size: 

                2275    1008     380
 
AfeI AGC'GCT

Cuts at:      0    2467    3663 
   Size:       2467    1196
 
AflII C'TTAA_G

Cuts at:      0     334    3663 
   Size:        334    3329
 
AflIII A'CryG_T

Cuts at:      0     481    1810    3663 
   Size:        481    1329    1853

  Fragments arranged by size: 

                1853    1329     481
 
AleI CACnn'nnGTG

Cuts at:      0    1632    1655    3663 
   Size:       1632      23    2008

  Fragments arranged by size: 

                2008    1632      23
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    3174    3206    3663 
   Size:       3174      32     457

  Fragments arranged by size: 

                3174     457      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     321     552    1092    2343    2761    3538    3663 
   Size:        321     231     540    1251     418     777     125

  Fragments arranged by size: 

                1251     777     540     418     321     231     125
 
ApaI G_GGCC'C

Cuts at:      0      52    1754    2507    3663 
   Size:         52    1702     753    1156

  Fragments arranged by size: 

                1702    1156     753      52
 
ApaLI G'TGCA_C

Cuts at:      0    3402    3663 
   Size:       3402     261
 
ApoI r'AATT_y

Cuts at:      0     523    1450    2074    2531    3663 
   Size:        523     927     624     457    1132

  Fragments arranged by size: 

                1132     927     624     523     457
 
AvaI C'yCGr_G

Cuts at:      0     412    1747    3079    3663 
   Size:        412    1335    1332     584

  Fragments arranged by size: 

                1335    1332     584     412
 
BamHI G'GATC_C

Cuts at:      0    3552    3663 
   Size:       3552     111
 
BanI G'GyrC_C

Cuts at:      0     157     638    1634    2317    3663 
   Size:        157     481     996     683    1346

  Fragments arranged by size: 

                1346     996     683     481     157
 
BanII G_rGCy'C

Cuts at:      0      52     835    1493    1748    1754    1956    2507    2768 
   Size:         52     783     658     255       6     202     551     261

Cuts at:   2768    3061    3663 
   Size:        293     602

  Fragments arranged by size: 

                 783     658     602     551     293     261     255     202
                  52       6
 
BbeI G_GCGC'C

Cuts at:      0     161    2321    3663 
   Size:        161    2160    1342

  Fragments arranged by size: 

                2160    1342     161
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1415    3663 
   Size:       1415    2248
 
BbvCI CC'TCA_GC

Cuts at:      0     545    1456    3596    3663 
   Size:        545     911    2140      67

  Fragments arranged by size: 

                2140     911     545      67
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3175    3209    3323    3357    3663 
   Size:       3175      34     114      34     306

  Fragments arranged by size: 

                3175     306     114      34      34
 
BclI T'GATC_A

Cuts at:      0    1067    1149    2175    3663 
   Size:       1067      82    1026    1488

  Fragments arranged by size: 

                1488    1067    1026      82
 
BfrBI ATG'CAT

Cuts at:      0     703    3663 
   Size:        703    2960
 
BglI GCCn_nnn'nGGC

Cuts at:      0      34    3663 
   Size:         34    3629
 
Bme1580I G_kGCm'C

Cuts at:      0      52    1319    1754    2507    3406    3663 
   Size:         52    1267     435     753     899     257

  Fragments arranged by size: 

                1267     899     753     435     257      52
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      82    3663 
   Size:         82    3581
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79    1076    2340    2430    2652    2864    3663 
   Size:         79     997    1264      90     222     212     799

  Fragments arranged by size: 

                1264     997     799     222     212      90      79
 
Bpu10I CC'TnA_GC

Cuts at:      0     135     545    1456    3110    3596    3663 
   Size:        135     410     911    1654     486      67

  Fragments arranged by size: 

                1654     911     486     410     135      67
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1429    1722    2702    3456    3663 
   Size:       1429     293     980     754     207

  Fragments arranged by size: 

                1429     980     754     293     207
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1654    3005    3316    3663 
   Size:       1654    1351     311     347

  Fragments arranged by size: 

                1654    1351     347     311
 
BsaAI yAC'GTr

Cuts at:      0     482     600    3663 
   Size:        482     118    3063

  Fragments arranged by size: 

                3063     482     118
 
BsaHI Gr'CG_yC

Cuts at:      0     158    2318    3663 
   Size:        158    2160    1345

  Fragments arranged by size: 

                2160    1345     158
 
BsaWI w'CCGG_w

Cuts at:      0    1396    1518    2295    3498    3663 
   Size:       1396     122     777    1203     165

  Fragments arranged by size: 

                1396    1203     777     165     122
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     445     475     559     589    2770    2800    3174    3204 
   Size:        445      30      84      30    2181      30     374      30

Cuts at:   3204    3217    3247    3663 
   Size:         13      30     416

  Fragments arranged by size: 

                2181     445     416     374      84      30      30      30
                  30      30      13
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     450     453     637    1835    3037    3663 
   Size:        450       3     184    1198    1202     626

  Fragments arranged by size: 

                1202    1198     626     450     184       3
 
BseYI C'CCAG_C

Cuts at:      0    2443    3345    3663 
   Size:       2443     902     318

  Fragments arranged by size: 

                2443     902     318
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     274     307     439    1985    2662    3255    3663 
   Size:        274      33     132    1546     677     593     408

  Fragments arranged by size: 

                1546     677     593     408     274     132      33
 
BsiEI CG_ry'CG

Cuts at:      0     125     265    3663 
   Size:        125     140    3398

  Fragments arranged by size: 

                3398     140     125
 
BsiHKAI G_wGCw'C

Cuts at:      0     835    1243    1748    2656    2935    3406    3663 
   Size:        835     408     505     908     279     471     257

  Fragments arranged by size: 

                 908     835     505     471     408     279     257
 
BsmI GAATG_Cn'

Cuts at:      0     279    2913    3663 
   Size:        279    2634     750

  Fragments arranged by size: 

                2634     750     279
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2509    2664    3663 
   Size:       2509     155     999

  Fragments arranged by size: 

                2509     999     155
 
Bsp1286I G_dGCh'C

Cuts at:      0      52     835    1243    1319    1493    1748    1754    1956 
   Size:         52     783     408      76     174     255       6     202

Cuts at:   1956    2507    2656    2768    2935    3061    3406    3663 
   Size:        551     149     112     167     126     345     257

  Fragments arranged by size: 

                 783     551     408     345     257     255     202     174
                 167     149     126     112      76      52       6
 
BspEI T'CCGG_A

Cuts at:      0    1518    3663 
   Size:       1518    2145
 
BspHI T'CATG_A

Cuts at:      0    1305    2178    2588    3663 
   Size:       1305     873     410    1075

  Fragments arranged by size: 

                1305    1075     873     410
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2337    2628    3334    3663 
   Size:       2337     291     706     329

  Fragments arranged by size: 

                2337     706     329     291
 
BsrDI GCAATG_nn'

Cuts at:      0     430    1935    2093    3385    3663 
   Size:        430    1505     158    1292     278

  Fragments arranged by size: 

                1505    1292     430     278     158
 
BsrFI r'CCGG_y

Cuts at:      0    3155    3461    3663 
   Size:       3155     306     202

  Fragments arranged by size: 

                3155     306     202
 
BsrGI T'GTAC_A

Cuts at:      0     605    3359    3663 
   Size:        605    2754     304

  Fragments arranged by size: 

                2754     605     304
 
BssSI C'ACGA_G

Cuts at:      0     892    3663 
   Size:        892    2771
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    3647    3663 
   Size:       3647      16
 
BstBI TT'CG_AA

Cuts at:      0    2373    3663 
   Size:       2373    1290
 
BstEII G'GTnAC_C

Cuts at:      0     586    3663 
   Size:        586    3077
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     379    2816    3663 
   Size:        379    2437     847

  Fragments arranged by size: 

                2437     847     379
 
BstYI r'GATC_y

Cuts at:      0     629    2864    3552    3663 
   Size:        629    2235     688     111

  Fragments arranged by size: 

                2235     688     629     111
 
Bsu36I CC'TnA_GG

Cuts at:      0    1466    3663 
   Size:       1466    2197
 
BtgI C'CryG_G

Cuts at:      0     128     642    1764    1989    3391    3663 
   Size:        128     514    1122     225    1402     272

  Fragments arranged by size: 

                1402    1122     514     272     225     128
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1284    3663 
   Size:       1284    2379
 
BtsI GCAGTG_nn'

Cuts at:      0    1842    2745    2912    3406    3663 
   Size:       1842     903     167     494     257

  Fragments arranged by size: 

                1842     903     494     257     167
 
ClaI AT'CG_AT

Cuts at:      0    2040    3663 
   Size:       2040    1623
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    3000    3035    3663 
   Size:       3000      35     628

  Fragments arranged by size: 

                3000     628      35
 
DraI TTT'AAA

Cuts at:      0     987    3663 
   Size:        987    2676
 
DraIII CAC_nnn'GTG

Cuts at:      0    1559    3663 
   Size:       1559    2104
 
EaeI y'GGCC_r

Cuts at:      0      83     660    1767    3663 
   Size:         83     577    1107    1896

  Fragments arranged by size: 

                1896    1107     577      83
 
EarI CTCTTCn'nnn_

Cuts at:      0    2024    3214    3314    3663 
   Size:       2024    1190     100     349

  Fragments arranged by size: 

                2024    1190     349     100
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1767    3663 
   Size:       1767    1896
 
EcoICRI GAG'CTC

Cuts at:      0     833    1746    3663 
   Size:        833     913    1917

  Fragments arranged by size: 

                1917     913     833
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     380    1076    1388    2340    2430    2652    2864 
   Size:         79     301     696     312     952      90     222     212

Cuts at:   2864    3663 
   Size:        799

  Fragments arranged by size: 

                 952     799     696     312     301     222     212      90
                  79
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     810    3052    3663 
   Size:        810    2242     611

  Fragments arranged by size: 

                2242     810     611
 
EcoO109I rG'GnC_Cy

Cuts at:      0      60     873    1751    2503    3663 
   Size:         60     813     878     752    1160

  Fragments arranged by size: 

                1160     878     813     752      60
 
EcoRI G'AATT_C

Cuts at:      0     523    3663 
   Size:        523    3140
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2904    3663 
   Size:       2904     759
 
FseI GG_CCGG'CC

Cuts at:      0    3465    3663 
   Size:       3465     198
 
HaeII r_GCGC'y

Cuts at:      0      94     161    2321    2469    3663 
   Size:         94      67    2160     148    1194

  Fragments arranged by size: 

                2160    1194     148      94      67
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     445     477     711     743    1052    1059    1084    1091 
   Size:        445      32     234      32     309       7      25       7

Cuts at:   1091    1134    1166    3537    3541    3569    3573    3663 
   Size:         43      32    2371       4      28       4      90

  Fragments arranged by size: 

                2371     445     309     234      90      43      32      32
                  32      28      25       7       7       4       4
 
KasI G'GCGC_C

Cuts at:      0     157    2317    3663 
   Size:        157    2160    1346

  Fragments arranged by size: 

                2160    1346     157
 
KpnI G_GTAC'C

Cuts at:      0     642    3663 
   Size:        642    3021
 
MscI TGG'CCA

Cuts at:      0      85    3663 
   Size:         85    3578
 
MslI CAynn'nnrTG

Cuts at:      0     758     961    1015    1151    1632    1655    3390    3663 
   Size:        758     203      54     136     481      23    1735     273

  Fragments arranged by size: 

                1735     758     481     273     203     136      54      23
            
 
MspA1I CmG'CkG

Cuts at:      0      99     130     155     164     636     856    1908    2314 
   Size:         99      31      25       9     472     220    1052     406

Cuts at:   2314    3663 
   Size:       1349

  Fragments arranged by size: 

                1349    1052     472     406     220      99      31      25
                   9
 
NaeI GCC'GGC

Cuts at:      0    3157    3463    3663 
   Size:       3157     306     200

  Fragments arranged by size: 

                3157     306     200
 
NarI GG'CG_CC

Cuts at:      0     158    2318    3663 
   Size:        158    2160    1345

  Fragments arranged by size: 

                2160    1345     158
 
NgoMIV G'CCGG_C

Cuts at:      0    3155    3461    3663 
   Size:       3155     306     202

  Fragments arranged by size: 

                3155     306     202
 
NsiI A_TGCA'T

Cuts at:      0     705    3663 
   Size:        705    2958
 
NspI r_CATG'y

Cuts at:      0    1814    2828    3663 
   Size:       1814    1014     835

  Fragments arranged by size: 

                1814    1014     835
 
PciI A'CATG_T

Cuts at:      0    1810    3663 
   Size:       1810    1853
 
PfoI T'CCnGG_A

Cuts at:      0    1804    1832    2336    3663 
   Size:       1804      28     504    1327

  Fragments arranged by size: 

                1804    1327     504      28
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3174    3206    3663 
   Size:       3174      32     457

  Fragments arranged by size: 

                3174     457      32
 
PshAI GACnn'nnGTC

Cuts at:      0    2521    2997    3663 
   Size:       2521     476     666

  Fragments arranged by size: 

                2521     666     476
 
PsiI TTA'TAA

Cuts at:      0    2252    3663 
   Size:       2252    1411
 
PspOMI G'GGCC_C

Cuts at:      0      48    1750    2503    3663 
   Size:         48    1702     753    1160

  Fragments arranged by size: 

                1702    1160     753      48
 
PstI C_TGCA'G

Cuts at:      0    1360    1432    2348    2740    3119    3663 
   Size:       1360      72     916     392     379     544

  Fragments arranged by size: 

                1360     916     544     392     379      72
 
PvuI CG_AT'CG

Cuts at:      0     265    3663 
   Size:        265    3398
 
PvuII CAG'CTG

Cuts at:      0      99     856    1908    2314    3663 
   Size:         99     757    1052     406    1349

  Fragments arranged by size: 

                1349    1052     757     406      99
 
SacI G_AGCT'C

Cuts at:      0     835    1748    3663 
   Size:        835     913    1915

  Fragments arranged by size: 

                1915     913     835
 
SacII CC_GC'GG

Cuts at:      0     131    3663 
   Size:        131    3532
 
SfcI C'TryA_G

Cuts at:      0     570    1356    1428    2344    2736    3115    3160    3663 
   Size:        570     786      72     916     392     379      45     503

  Fragments arranged by size: 

                 916     786     570     503     392     379      72      45
            
 
SfoI GGC'GCC

Cuts at:      0     159    2319    3663 
   Size:        159    2160    1344

  Fragments arranged by size: 

                2160    1344     159
 
SmlI C'TyrA_G

Cuts at:      0     334    1444    1701    2717    3471    3663 
   Size:        334    1110     257    1016     754     192

  Fragments arranged by size: 

                1110    1016     754     334     257     192
 
SpeI A'CTAG_T

Cuts at:      0    2163    3663 
   Size:       2163    1500
 
SspI AAT'ATT

Cuts at:      0    1917    2290    3294    3663 
   Size:       1917     373    1004     369

  Fragments arranged by size: 

                1917    1004     373     369
 
StuI AGG'CCT

Cuts at:      0    2258    2836    3663 
   Size:       2258     578     827

  Fragments arranged by size: 

                2258     827     578
 
StyI C'CwwG_G

Cuts at:      0      86    1599    2582    2988    3663 
   Size:         86    1513     983     406     675

  Fragments arranged by size: 

                1513     983     675     406      86
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     111    2952    3199    3663 
   Size:        111    2841     247     464

  Fragments arranged by size: 

                2841     464     247     111
 
TatI w'GTAC_w

Cuts at:      0     605    2051    2821    3359    3663 
   Size:        605    1446     770     538     304

  Fragments arranged by size: 

                1446     770     605     538     304
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1680    1712    1800    1832    2796    2828    3014    3046 
   Size:       1680      32      88      32     964      32     186      32

Cuts at:   3046    3217    3249    3663 
   Size:        171      32     414

  Fragments arranged by size: 

                1680     964     414     186     171      88      32      32
                  32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    3435    3663 
   Size:       3435     228
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     640    2561    3663 
   Size:        640    1921    1102

  Fragments arranged by size: 

                1921    1102     640
 
XmnI GAAnn'nnTTC

Cuts at:      0    2887    3247    3663 
   Size:       2887     360     416

  Fragments arranged by size: 

                2887     416     360
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AfeI    AflII   AflIII     AleI     AloI
    AlwNI     ApaI    ApaLI     ApoI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI Bme1580I
     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrDI    BsrFI    BsrGI    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     EaeI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FseI    HaeII
    Hin4I     KasI     KpnI     MscI     MslI   MspA1I     NaeI     NarI
   NgoMIV     NsiI     NspI     PciI     PfoI     PpiI    PshAI     PsiI
   PspOMI     PstI     PvuI    PvuII     SacI    SacII     SfcI     SfoI
     SmlI     SpeI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AgeI     AhdI     AlfI     AscI     AseI
    AsiSI    AvrII     BaeI    BciVI    BglII     BlpI    BmgBI     BmtI
     BplI    BsaBI    BsiWI    BsrBI   BssHII  BstZ17I     DrdI     EagI
    EcoRV     FspI    FspAI   HincII  HindIII     HpaI     MfeI     MluI
     MmeI     NcoI     NdeI     NheI     NotI     NruI     PacI     PasI
    PflMI     PmeI     PmlI    PpuMI    PspXI     PsrI    RsrII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI
     SmaI    SnaBI     SphI     SrfI     SwaI     XbaI     XhoI     XmaI
     ZraI
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