Restriction map for pF1KB9482
##################################
# NO CUT ENZYMES (  106 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AgeI        Aor51HI     ApoI        AscI        
AseI        AsnI        Asp718I     BamHI       BbrPI       
BciVI       BfrI        BglII       BsaBI       BsaI        
BsaWI       BseAI       BsiEI       BsiWI       BsmAI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstXI       BstZ17I     Cfr10I      ClaI        
CpoI        DraI        DrdI        DsaI        EagI        
EclXI       Eco47III    Eco52I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FseI        HpaI        
KpnI        KspI        MamI        MfeI        MluI        
MroI        MslI        MunI        NaeI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacII       
SbfI        ScaI        SexAI       SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        XbaI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccI         1305  GTCGAC               GT^[AC][GT]AC
AccII         198  CGCG                 CG^CG
AhdI          814  GACTACATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        418  GTGCAC               G^TGCAC
AlwNI        1288  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          949  CCTGAGG              CC^T[ACGT]AGG
ApaI          889  GGGCCC               GGGCC^C
ApaLI         418  GTGCAC               G^TGCAC
AspEI         814  GACTACATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          103  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         614  TGCGCA               TGC^GCA
AvrII           9  CCTAGG               C^CTAGG
BalI          719  TGGCCA               TGG^CCA
BbvCI        1073  CCTCAGC              CCTCAGC(-5/-2)
BclI         1517  TGATCA               T^GATCA
BlnI            9  CCTAGG               C^CTAGG
BlpI         1449  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1449  GCTGAGC              GC^T[ACGT]AGC
BsaAI         120  TACGTG               [CT]AC^GT[GA]
BsgI         1354  GTGCAG               GTGCAG(16/14)
BspLU11I      818  ACATGT               A^CATGT
BstUI         198  CGCG                 CG^CG
BstYI        1484  AGATCC               [GA]^GATC[CT]
Bsu36I        949  CCTGAGG              CC^T[ACGT]AGG
CelII        1449  GCTGAGC              GC^T[ACGT]AGC
DraIII        381  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I      814  GACTACATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        949  CCTGAGG              CC^T[ACGT]AGG
FbaI         1517  TGATCA               T^GATCA
FspI          614  TGCGCA               TGC^GCA
HgaI         1101  GACGC                GACGC(5/10)
HindIII       220  AAGCTT               A^AGCTT
MflI         1484  AGATCC               [GA]^GATC[CT]
MluNI         719  TGGCCA               TGG^CCA
MscI          719  TGGCCA               TGG^CCA
MseI           92  TTAA                 T^TAA
MvnI          198  CGCG                 CG^CG
NspI          818  ACATGT               [GA]CATG^[CT]
PaeR7I       1222  CTCGAG               C^TCGAG
PflFI         103  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PstI         1253  CTGCAG               CTGCA^G
SacI         1137  GAGCTC               GAGCT^C
SalI         1305  GTCGAC               G^TCGAC
SapI          847  GAAGAGC              GCTCTTC(1/4)
SfcI         1253  CTGCAG               C^T[GA][CT]AG
SfiI          399  GGCCTTCGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tru9I          92  TTAA                 T^TAA
Tsp509I       433  AATT                 ^AATT
Tth111I       103  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         1287  CCAGAGCCTGTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1222  CTCGAG               C^TCGAG
XhoII        1484  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AfaI          249  GTAC                 GT^AC
              429  GTAC                
AlwI         1485  GATCC                GGATC(4/5)
              549  GGATC               
Asp700I       513  GAACCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1422  GAAAAAGTTC          
AspHI         418  GTGCAC               G[AT]GC[AT]^C
             1137  GAGCTC              
BanII         889  GGGCCC               G[GA]GC[CT]^C
             1137  GAGCTC              
BbeI          711  GGCGCC               GGCGC^C
              870  GGCGCC              
BbsI         1098  GAAGAC               GAAGAC(2/6)
             1440  GAAGAC              
BcgI          605  CGAGCGAGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1245  CGAGATGCCTGC        
BcnI          300  CCGGG                CC^[GC]GG
              449  CCCGG               
BfaI           10  CTAG                 C^TAG
               17  CTAG                
BpuAI        1098  GAAGAC               GAAGAC(2/6)
             1440  GAAGAC              
BsiHKAI       418  GTGCAC               G[AT]GC[AT]^C
             1137  GAGCTC              
BspMI        1415  ACCTGC               ACCTGC(4/8)
             1356  GCAGGT              
BsrBI         142  GAGCGG               CCGCTC(-3/-3)
             1117  GAGCGG              
EaeI          591  TGGCCG               [CT]^GGCC[GA]
              719  TGGCCA              
EarI         1081  CTCTTC               CTCTTC(1/4)
              847  GAAGAG              
HaeII         711  GGCGCC               [GA]GCGC^[CT]
              870  GGCGCC              
KasI          711  GGCGCC               G^GCGCC
              870  GGCGCC              
Ksp632I      1081  CTCTTC               CTCTTC(1/4)
              847  GAAGAG              
MaeI           10  CTAG                 C^TAG
               17  CTAG                
NarI          711  GGCGCC               GG^CGCC
              870  GGCGCC              
NciI          300  CCGGG                CC^[GC]GG
              449  CCCGG               
PflMI         621  CCACGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              804  CCAAGAGGTGG         
PpuMI        1281  GGGACCC              [GA]G^G[AT]CC[CT]
             1412  AGGACCT             
RsaI          249  GTAC                 GT^AC
              429  GTAC                
SfoI          711  GGCGCC               GGC^GCC
              870  GGCGCC              
TfiI          126  GAATC                G^A[AT]TC
              190  GAATC               
Tsp45I         31  GTCAC                ^GT[GC]AC
             1360  GTGAC               
Van91I        621  CCACGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              804  CCAAGAGGTGG         
XmnI          513  GAACCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1422  GAAAAAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   70 enzymes) #
#########################################

AciI        AcyI        AflIII      AluI        AvaI        
AvaII       BanI        BbvI        BglI        BmyI        
BpmI        BsaHI       BsaJI       BseRI       BsiYI       
BslI        BsmFI       BsoBI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        Hin1I       HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        MaeII       MaeIII      MboI        MboII       
MnlI        MspA1I      MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       PleI        Sau3AI      
Sau96I      ScrFI       SfaNI       SmlI        StuI        
StyI        TaqI        TseI        TspRI       TthHB8I     
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